Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

The insecticidal spider toxin SFI1 is a knottin peptide that blocks the pore of insect voltage-gated sodium channels via a large β-hairpin loop

(2015) Niraj S. Bende et al.   FEBS Journal

A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide

(2015) Herlinda Clement et al.   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a

(2014) Niraj S. Bende et al.   Nature Communications

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

(2014) Natalie J. Saez et al.   Jove-Journal of Visualized Experiments

High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli

(2013) Hervé Nozach et al.   Microbial Cell Factories

Production of Recombinant Disulfide-Rich Venom Peptides for Structural and Functional Analysis via Expression in the Periplasm of E. coli

(2013) Julie K. Klint et al.   PLoS One

Discovery of a selective NaV1.7 inhibitor from centipede venom with analgesic efficacy exceeding morphine in rodent pain models

(2013) S. Yang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional Expression in Escherichia coli of the Disulfide-Rich Sea Anemone Peptide APETx2, a Potent Blocker of Acid-Sensing Ion Channel 3

(2012) Raveendra Anangi et al.   Marine Drugs

High-throughput protein expression screening and purification in Escherichia coli

(2011) Renaud Vincentelli et al.   METHODS

Venomics as a drug discovery platform

(2009) Pierre Escoubas et al.   Expert Review of Proteomics

You're one in a googol: optimizing genes for protein expression

(2009) M. Welch et al.   Journal of the Royal Society Interface

Gene synthesis demystified

(2008) Michael J. Czar et al.   TRENDS IN BIOTECHNOLOGY