Creating a completely “cell-free” system for protein synthesis

LyophilizedEscherichia coli-based cell-free systems for robust, high-density, long-term storage

(2016) Mark Thomas Smith et al.   BIOTECHNIQUES

Enhanced Enzyme Stability Through Site-Directed Covalent Immobilization

(2015) Jeffrey Chun Yu Wu et al.   JOURNAL OF BIOTECHNOLOGY

Semisynthetic tRNA Complement Mediates in Vitro Protein Synthesis

(2015) Zhenling Cui et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

(2015) Yong-Chan Kwon et al.   Scientific Reports

Beta-Lactam Antibiotics Induce a Lethal Malfunctioning of the Bacterial Cell Wall Synthesis Machinery

(2014) Hongbaek Cho et al.   CELL

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

The emerging age of cell-free synthetic biology

(2014) Mark Thomas Smith et al.   FEBS LETTERS

Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates

(2013) Prashanta Shrestha et al.   New Biotechnology

Enhanced protein stability through minimally invasive, direct, covalent, and site-specific immobilization

(2012) Mark T. Smith et al.   BIOTECHNOLOGY PROGRESS

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Preparative Scale Cell-free Production and Quality Optimization of MraY Homologues in Different Expression Modes

(2011) Yi Ma et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cell-free synthetic biology: Thinking outside the cell

(2011) C. Eric Hodgman et al.   METABOLIC ENGINEERING

An integrated cell-free metabolic platform for protein production and synthetic biology

(2008) Michael C Jewett et al.   Molecular Systems Biology