Article
Biochemical Research Methods
Corrie R. Belanger, Robert E. W. Hancock
Summary: This study presents a protocol for testing physiologically relevant infection conditions in minimal inhibitory concentration (MIC) assays, which can be used to assess the susceptibility of bacterial isolates and novel antimicrobial drugs under conditions more similar to clinical infections. The protocol can be applied to most physiologically relevant liquid medium and aerobic pathogens, taking 3-4 days to complete, and may provide a more accurate assessment of the bactericidal effects of new drugs.
Article
Microbiology
Andras Kallai, Marta Kelemen, Noemi Molnar, Adrienn Tropotei, Balazs Hauser, Zsolt Ivanyi, Janos Gal, Erzsebet Ligeti, Katalin Kristof, Akos M. Lorincz
Summary: The study developed a flow cytometry-based antibiotic susceptibility testing (AST) method, which can produce reliable results a workday earlier than the traditional microdilution method. Results showed that the method is able to detect bacterial growth accurately after 4 hours of incubation, with high consistency compared to the microdilution method.
MICROBIOLOGY SPECTRUM
(2021)
Article
Medicine, General & Internal
Jonatan A. N. Tillander, Karin Rilby, Karin Svensson Malchau, Susann Skovbjerg, Erika Lindberg, Ola Rolfson, Margarita Trobos
Summary: Prosthetic joint infections (PJIs) are a serious issue for patients and healthcare organizations, and current treatment methods have limited success rates. This study aims to evaluate the feasibility of an antibiotic regimen based on the minimum biofilm eradication concentration (MBEC) and explore the relationship between microbial properties, antimicrobial resistance, and infection outcomes.
Article
Environmental Sciences
Sirinthrar Wandee, Rathborey Chan, Wilai Chiemchaisri, Chart Chiemchaisri
Summary: The study found that in open pig farming systems, there is a high level of E. coli populations, antibiotic contamination in supplied water, and additional antibiotic usage, such as neomycin or colistin. Additionally, an increased occurrence of ARE was found in anaerobic digestion and waste stabilisation ponds, but they were not well correlated with their residual antibiotic concentrations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Infectious Diseases
Tejas Suresh Khire, Wei Gao, Brian Bales, Kuangwen Hsieh, Greg Grossmann, Dong Jin M. Park, Christine O'Keefe, Arnyah Brown-Countess, Sara Peterson, Fan-En Chen, Ralf Lenigk, Alex Trick, Tza-Huei Wang, Christopher Puleo, Gabriele Bianco
Summary: Antimicrobial resistance is a global threat caused by the incorrect and overuse of antibiotics, resulting in the evolution of multi- and extreme drug-resistant bacterial strains. This study aims to provide phenotypic antimicrobial susceptibility testing information by combining short growth periods with PCR assays to predict minimum inhibitory concentration values of antibiotic treatment. The use of a single-vessel reaction system and microfluidic manifold allows for rapid point-of-care diagnostics.
Article
Spectroscopy
Mohammad Javad Jafari, Mohsen Golabi, Thomas Ederth
Summary: The use of infrared attenuated total reflection (IR-ATR) provides a simple method for assessing bacterial susceptibility to antibiotics by monitoring bacterial metabolic activities. Principal component analysis can quickly discriminate antimicrobial resistance based on obtained spectra.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Microbiology
Troels Ronco, Line H. Kappel, Maria F. Aragao, Niccolo Biagi, Soren Svenningsen, Jorn B. Christensen, Anders Permin, Lasse Saaby, Kim Holmstrom, Janne K. Klitgaard, Artur J. Sabat, Viktoria Akkerboom, Monica Monaco, Marco Tinelli, Alexander W. Friedrich, Bimal Jana, Rikke H. Olsen
Summary: Multidrug-resistant pathogens are a serious global issue, and novel antimicrobials with new modes of action are urgently needed. In this study, a phenothiazine derivative (JBC 1847) was found to exhibit high antimicrobial activity against Staphylococcus aureus. The primary mechanism of action of JBC 1847 was the depolarization of the cell membrane, leading to the dissipation of the proton motive force and affecting essential bacterial processes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
V. Lucien Barnes, Douglas M. Heithoff, Scott P. Mahan, John K. House, Michael J. Mahan
Summary: This article presents a protocol for evaluating the minimum inhibitory concentration (MIC) values of clinically relevant antibiotics against bacterial isolates cultured in standard bacteriologic medium and in mammalian cell culture medium. The protocol can potentially optimize the use of existing antibiotics while enhancing efforts to discover new ones.
Review
Infectious Diseases
Ina Gajic, Jovana Kabic, Dusan Kekic, Milos Jovicevic, Marina Milenkovic, Dragana Mitic Culafic, Anika Trudic, Lazar Ranin, Natasa Opavski
Summary: Antimicrobial resistance poses a significant threat to global public health. Accurate and rapid detection of antibiotic resistance and appropriate treatment methods are crucial for controlling its emergence. However, traditional testing methods are time-consuming and costly, highlighting the need for innovative approaches.
Article
Health Care Sciences & Services
Clara Grazian
Summary: Antimicrobial resistance poses a significant threat to global public health. Researchers are using whole-genome sequencing to develop personalized treatments and improve prediction of resistance and treatment recommendations. Robust methods are needed to identify resistance-associated mutations.
STATISTICAL METHODS IN MEDICAL RESEARCH
(2023)
Article
Microbiology
Amanda Holstad Singleton, Olaug Elisabeth Torheim Bergum, Caroline Krogh Sogaard, Lisa Marie Rost, Cecilie Elisabeth Olsen, Fredrik Heen Blindheim, Synnove Brandt Raeder, Frithjof A. Bjornstad, Eirik Sundby, Bard Helge Hoff, Per Bruheim, Marit Otterlei
Summary: The increasing number of infections caused by antibiotic resistant bacteria in recent decades has led to a demand for new antibiotics and drug combinations to reduce resistance development. The combination of the antimicrobial peptide BTP-001 and the pyrrolopyrimidine JK-274 has shown a strong additive effect, likely due to the activation of multiple stress responses. These findings demonstrate the potential of JK-274 and BTP-001 as antibiotic candidates and call for further studies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Anno de Jong, Myriam Youala, Ulrich Klein, Farid El Garch, Shabbir Simjee, Hilde Moyaert, Markus Rose, Anne V. Gautier-Bouchardon, Salvatore Catania, Kannan Ganapathy, Miklos Gyuranecz, Franca Moller Palau-Ribes, Andrew Pridmore, Roger D. Ayling
Summary: This study presents the results of the first international antimicrobial susceptibility programme for mycoplasma pathogens isolated from poultry, providing a baseline of novel data for future monitoring of antimicrobial resistance in poultry Mycoplasma species. The study indicates variations in susceptibilities based on host species, Mycoplasma species, and country of origin, and highlights the importance of selecting effective antimicrobial agents for targeted therapeutic treatments.
Review
Agriculture, Dairy & Animal Science
H. Taiyari, N. M. Faiz, J. Abu, Z. Zakaria
Summary: Although antibiotic treatment cannot eradicate avian mycoplasmosis, it can reduce the number of deaths. One major challenge of antibiotic treatments is the development of antimicrobial resistance among field isolates. Regular determination of minimum inhibitory concentrations of antibiotics is necessary to optimize treatment dosages.
JOURNAL OF APPLIED POULTRY RESEARCH
(2021)
Article
Chemistry, Medicinal
Marita Meurer, Deborah A. O'Neil, Emma Lovie, Laura Simpson, Marcelo D. T. Torres, Cesar De la Fuente-Nunez, Alfredo M. Angeles-Boza, Christin Kleinsorgen, Derry K. Mercer, Maren Von Koeckritz-Blickwede
Summary: Experts at IMAP and GRC conferences discussed the need for optimized and standardized test systems for novel antimicrobial peptides, and a survey was conducted to gather opinions on this topic. Based on the survey results, suggestions for future improvements in the research field were made.
ACS INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Xiao Wu, Yuanyuan Shang, Weicong Ren, Wei Wang, Yufeng Wang, Zhongtan Xue, Shanshan Li, Yu Pang
Summary: The study determined the breakpoint of cycloserine susceptibility in MGIT and described the molecular characteristics of CS-resistant Mycobacterium tuberculosis isolates. Drug susceptibility testing and whole-genome sequencing revealed that mutations in alr and ald genes were associated with CS resistance. The proportion of CS resistance was higher in extensively drug-resistant TB compared to multidrug-resistant TB.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Dentistry, Oral Surgery & Medicine
K. P. Smith, R. D. Voogt, T. Ruiz, K. P. Mintz
MOLECULAR ORAL MICROBIOLOGY
(2016)
Article
Biochemical Research Methods
Kenneth P. Smith, Julia G. Fields, Richard D. Voogt, Bin Deng, Ying-Wai Lam, Keith P. Mintz
Article
Biochemical Research Methods
Kenneth P. Smith, James E. Kirby
ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES
(2016)
Article
Infectious Diseases
Kenneth P. Smith, James E. Kirby
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2016)
Editorial Material
Microbiology
Kenneth P. Smith, James E. Kirby
FUTURE MICROBIOLOGY
(2016)
Article
Microbiology
Kenneth P. Smith, Thea Brennan-Krohn, Susan Weir, James E. Kirby
JOURNAL OF CLINICAL MICROBIOLOGY
(2017)
Article
Microbiology
Kenneth P. Smith, James E. Kirby
JOURNAL OF CLINICAL MICROBIOLOGY
(2016)
Article
Microbiology
Kenneth P. Smith, Teresa Ruiz, Keith P. Mintz
Article
Infectious Diseases
Thea Brennan-Krohn, Katherine A. Truelson, Kenneth P. Smith, James E. Kirby
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2017)
Article
Microbiology
Kenneth P. Smith, Anthony D. Kang, James E. Kirby
JOURNAL OF CLINICAL MICROBIOLOGY
(2018)
Article
Biochemical Research Methods
Kenneth P. Smith, David L. Richmond, Thea Brennan-Krohn, Hunter L. Elliott, James E. Kirby
Article
Dentistry, Oral Surgery & Medicine
K. P. Smith, J. G. Fields, R. D. Voogt, B. Deng, Y-W. Lam, K. P. Mintz
MOLECULAR ORAL MICROBIOLOGY
(2015)
Article
Infectious Diseases
Alice Chen, Kenneth P. Smith, Betsy A. Whitfield, Paola C. Zucchi, Todd M. Lasco, Tiffany E. Bias, James E. Kirby, Elizabeth B. Hirsch
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2017)
Article
Infectious Diseases
Anthony D. Kang, Kenneth P. Smith, George M. Eliopoulos, Anders H. Berg, Christopher Mccoy, James E. Kirby
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2017)
