Article
Cell Biology
Ana Krotenberg Garcia, Arianna Fumagalli, Huy Quang Le, Rene Jackstadt, Tamsin Rosemary Margaret Lannagan, Owen J. Sansom, Jacco van Rheenen, Saskia J. E. Suijkerbuijk
Summary: Competitive cell interactions between cancer cells and wild-type cells lead to the active elimination of normal cells and promotion of cancer cell proliferation. This process is driven by cell-state change in wild-type cells and activation of JNK signaling, ultimately resulting in the out-competition of normal cells by cancer cells.
Article
Oncology
Chen Hang, Shanojie Zhao, Tiejun Wang, Yan Zhang
Summary: In this study, UBE3C was identified as an oncogenic factor in breast cancer growth and metastasis. High nuclear UBE3C expression in breast cancer tissues was associated with worse overall survival. Knockdown of UBE3C inhibited cell proliferation, migration and invasion in breast cancer cells, while overexpression of UBE3C had the opposite effects. UBE3C promoted beta-catenin nuclear accumulation and activated the Wnt/beta-catenin signaling pathway.
CANCER CELL INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han
Summary: Nuclear S6K1 functions in the regulation of Wnt target gene expression and has an impact on cell proliferation and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Fangming Liu, Xiaochen Gai, Yuting Wu, Baohui Zhang, Xiaoyu Wu, Rongrong Cheng, Bufu Tang, Kezhuo Shang, Na Zhao, Weiwei Deng, Jie Chen, Zhengyi Zhang, Song Gu, Liang Zheng, Hongbing Zhang
Summary: This study reveals the oncogenic role of CTNNB1 gain-of-function mutations in liver cancer development and identifies the beta-catenin/AKT2/CAD signaling cascade as a potential therapeutic target for this type of liver cancer.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Kibrom M. Alula, Yaritza Delgado-Deida, Dakota N. Jackson, K. Venuprasad, Arianne L. Theiss
Summary: PHB1 plays a role in inhibiting the Wnt/β-catenin pathway to influence the development of intestinal tumorigenesis by regulating AXIN1 expression and the beta-catenin destruction complex. Induction of nuclear PHB1 trafficking provides a novel therapeutic option to influence AXIN1 expression and the beta-catenin destruction complex in Wnt-driven intestinal tumorigenesis.
Article
Biochemistry & Molecular Biology
Ying-chao Qin, Jia-yi Zhou, Min Zhu, Geng-xiu Zan, Chun-qi Gao, Hui-chao Yan, Xiang-guang Li, Xiu-qi Wang
Summary: This study demonstrates that L-glutamate (Glu) supplementation stimulates gut growth and intestinal stem cell (ISC) expansion in weaned piglets. The canonical Wnt signalling pathway is identified as a central regulator of intestinal epithelial development and ISC activity. The study also reveals that the Wnt transmembrane receptor Frizzled7 (FZD7) plays a crucial role in the integration of Glu signals and regulates ISC proliferation and differentiation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell & Tissue Engineering
Jia-yi Zhou, Deng-gui Huang, Chun-qi Gao, Hui-chao Yan, Shi-geng Zou, Xiu-qi Wang
Summary: The study found that STp inhibits intestinal stem cell expansion and disrupts intestinal mucosa integrity by downregulating the Wnt/beta-catenin signaling pathway.
Article
Multidisciplinary Sciences
Helen Tanton, Tomasz Sewastianik, Hyuk-Soo Seo, David Remillard, Roodolph St Pierre, Pratyusha Bala, Daulet Aitymbayev, Peter Dennis, Keith Adler, Ezekiel Geffken, Zoe Yeoh, Nicholas Vangos, Filip Garbicz, David Scott, Nilay Sethi, James Bradner, Sirano Dhe-Paganon, Ruben D. Carrasco
Summary: The study identified a specific small-molecule inhibitor that can suppress Wnt/beta-catenin signaling and slow down the growth of colorectal cancer. This finding is of great significance for the development of novel therapies for colorectal cancer.
Article
Cell & Tissue Engineering
Junquan Tian, Yuying Li, Xuetai Bao, Fan Yang, Xiongzhuo Tang, Qian Jiang, Yulong Yin, Kang Yao
Summary: Early weaning inhibits the self-renewal and function of intestinal stem cells, leading to intestinal atrophy. This is potentially due to the suppression of Wnt signaling and activation of proinflammatory cytokines. These findings may offer a potential therapeutic approach for alleviating intestinal problems caused by early weaning.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Clinical Neurology
Javier Jarazo, Kyriaki Barmpa, Jennifer Modamio, Claudia Saraiva, Sonia Sabate-Soler, Isabel Rosety, Anne Griesbeck, Florian Skwirblies, Gaia Zaffaroni, Lisa M. Smits, Jihui Su, Jonathan Arias-Fuenzalida, Jonas Walter, Gemma Gomez-Giro, Anna S. Monzel, Xiaobing Qing, Armelle Vitali, Gerald Cruciani, Ibrahim Boussaad, Francesco Brunelli, Christian Jager, Aleksandar Rakovic, Wen Li, Lin Yuan, Emanuel Berger, Giuseppe Arena, Silvia Bolognin, Ronny Schmidt, Christoph Schroeder, Paul M. A. Antony, Christine Klein, Rejko Kruger, Philip Seibler, Jens C. Schwamborn
Summary: This study investigated the differences between neurons derived from Parkinson's disease patients and controls, identifying potential pathways for targeted treatment. By correcting mutations and using a specific compound, improvements in metabolic properties and neuronal differentiation in patient-derived cells were observed. Treatment with a repurposed compound was shown to restore impaired dopaminergic differentiation in Parkinson's disease patient-derived cells.
MOVEMENT DISORDERS
(2022)
Article
Food Science & Technology
Yang Li, Junfei Chen, Dong Sun, Jingwei Liu, Zhe Wang, Aiwu Li
Summary: LGG administration can alleviate intestinal lesions in NEC by restoring intestinal flora, activating Wnt/β-catenin signaling pathway, promoting ISC regeneration, increasing the number of TJs, and improving intestinal barrier function.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Fan Feng, Zongqing Zhao, Yunfei Zhou, Yanhao Cheng, Xiujie Wu, Xueyuan Heng
Summary: The study revealed that overexpression of CUX1 in glioma promotes tumor progression and regulates the activity of the Wnt/beta-catenin signaling pathway. CUX1 could serve as a novel therapeutic target for glioma, and gene therapy targeting CUX1 may have potential benefits in the treatment of glioma.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yalan Dong, Heng Fan, Zhe Zhang, Feng Jiang, Mingyue Li, Haifeng Zhou, Weina Guo, Zili Zhang, Zhenyu Kang, Yang Gui, Zhexing Shou, Junyi Li, Rui Zhu, Yu Fu, Alexey Sarapultsev, Huafang Wang, Shanshan Luo, Ge Zhang, Desheng Hu
Summary: In this study, the therapeutic effect and mechanism of berberine (BBR) in treating DSS-induced colitis were investigated. It was found that BBR can reduce colon inflammation, regulate the abundance and composition of bacteria, restore the broken chemical and epithelial barriers, and modulate the expression of various immune factors. The protective effect of BBR was also observed through the enhancement of the Wnt/beta-catenin pathway. These findings suggest that BBR serves as a potential gut microbiota modulator and mucosal barrier protector for UC prevention and therapy.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Jiaqi He, Mengling Wang, Licai Yang, Hong Xin, Fan Bian, Gengru Jiang, Xuemei Zhang
Summary: This study demonstrates the therapeutic value of Astragaloside IV (AS IV) in treating intestinal dysfunction caused by long-term peritoneal dialysis (PD). AS IV protects the intestinal epithelial barrier by improving its permeability and up-regulating the expression of tight junction proteins. Additionally, AS IV activates the AKT-GSK3β-β-catenin signaling pathway to mediate its protective effects.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lingling Zhang, Ran Ren, Xue Yang, Yiman Ge, Xiajun Zhang, Hongping Yuan
Summary: This study found that EGR1 promotes the malignant behaviors of LC cells through mediating the miRNA-675/SESN3/Wnt/β-catenin axis, while knockdown of EGR1 weakens proliferation and invasiveness of LC cells.
Article
Oncology
Philip Bischoff, Alexandra Trinks, Jennifer Wiederspahn, Benedikt Obermayer, Jan Patrick Pett, Philipp Jurmeister, Aron Elsner, Tomasz Dziodzio, Jens-Carsten Rueckert, Jens Neudecker, Christine Falk, Dieter Beule, Christine Sers, Markus Morkel, David Horst, Frederick Klauschen, Nils Bluethgen
Summary: This study used single-cell RNA sequencing to analyze lung carcinoid tumors and normal lung tissue, revealing cellular heterogeneity in the tumor cells. Noninflammatory myeloid cells were enriched in the immune microenvironment, while tumor-associated endothelial cells had distinct gene expression profiles. Additionally, the study found that single-cell gene signatures of pericytes and myofibroblasts had prognostic value.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Genetics & Heredity
Tincy Simon, Pamela Riemer, Armin Jarosch, Katharina Detjen, Annunziata Di Domenico, Felix Bormann, Andrea Menne, Slim Khouja, Nanna Monje, Liam H. Childs, Dido Lenze, Ulf Leser, Florian Rossner, Markus Morkel, Nils Bluethgen, Marianne Pavel, David Horst, David Capper, Ilaria Marinoni, Aurel Perren, Soulafa Mamlouk, Christine Sers
Summary: Pancreatic neuroendocrine neoplasms (PanNENs) can be classified into two subclasses: well-differentiated PanNETs and poorly-differentiated high-grade PanNECs. Recent studies have shown that high-grade PanNECs originate from exocrine cells, distinguishing them in cell lineage from other endocrine-origin PanNETs. This study provides a method to clearly distinguish PanNECs from G3 PanNETs, improving patient stratification.
Article
Chemistry, Analytical
Yang Zhang, Benjamin Dreyer, Natalia Govorukhina, Alexander M. Heberle, Sasa Koncarevic, Christoph Krisp, Christiane A. . Opitz, Pauline Pfander, Rainer Bischoff, Hartmut Schlueter, Marcel Kwiatkowski, Kathrin Thedieck, Peter L. Horvatovich
Summary: The study compares three different quantitation techniques for tumor tissue phosphoproteomics and highlights the strengths and limitations of each method. The choice of quantitative methodology is critical for study design and comparison of published cancer phosphoproteomes. The results provide a resource for the design and analysis of quantitative phosphoproteomic studies in cancer research and diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Cardiac & Cardiovascular Systems
Mahmoud Abdellatif, Viktoria Trummer-Herbst, Alexander Martin Heberle, Alina Humnig, Tobias Pendl, Sylvere Durand, Giulia Cerrato, Sebastian J. Hofer, Moydul Islam, Julia Voglhuber, Jose Miguel Ramos Pittol, Oliver Kepp, Gerald Hoefler, Albrecht Schmidt, Peter P. Rainer, Daniel Scherr, Dirk von Lewinski, Egbert Bisping, Julie R. McMullen, Abhinav Diwan, Tobias Eisenberg, Frank Madeo, Kathrin Thedieck, Guido Kroemer, Simon Sedej
Summary: This study suggests a biphasic relationship between insulin-like growth factor 1 receptor (IGF1R) signaling and cardiac health. Higher IGF1R signaling in young mice leads to declining cardiac function and shorter lifespan, while lower IGF1R signaling improves cardiac function and extends lifespan in aging mice.
Article
Oncology
Sofya Marchenko, Iris Piwonski, Inga Hoffmann, Bruno Valentin Sinn, Catarina Alisa Kunze, Nanna Monje, Jonathan Pohl, Hagen Kulbe, Wolfgang Daniel Schmitt, Sylvia Darb-Esfahani, Elena Ioana Braicu, Ann-Christin von Bruenneck, Jalid Sehouli, Carsten Denkert, David Horst, Korinna Joehrens, Eliane Tabea Taube
Summary: The study found that higher proportions of regulatory T cells (Tregs) and lower ratios of T helper 17 cells (Th17 cells) were associated with better overall survival in high-grade serous tubo-ovarian carcinoma. This research is important for understanding the impact of the tumor microenvironment on tumor development.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Review
Oncology
Miguel Enrique Alberto Vilchez, Eva Pachmayr, Alexander Arnold, Safak Guel-Klein, Andreas Brandl, Beate Rau
Summary: Malignant mesotheliomas are rare diseases, and cases with synchronous involvement of both the pleura and peritoneum are even scarcer. There is no standardized treatment recommendation for children with mesothelioma. Studies have found that mesothelioma can spread between the pleura and peritoneum. A radical two-stage surgical approach in combination with locoregional chemotherapy has shown effectiveness in treating patients.
INDIAN JOURNAL OF SURGICAL ONCOLOGY
(2023)
Article
Oncology
Craig M. Goodwin, Andrew M. Waters, Jennifer E. Klomp, Sehrish Javaid, Kirsten L. Bryant, Clint A. Stalnecker, Kristina Drizyte-Miller, Bjoern Papke, Runying Yang, Amber M. Amparo, Irem Ozkan-Dagliyan, Elisa Baldelli, Valerie Calvert, Mariaelena Pierobon, Jessica A. Sorrentino, Andrew P. Beelen, Natalie Bublitz, Mareen Luethen, Kris C. Wood, Emanuel F. Petricoin III, Christine Sers, Autumn J. McRee, Adrienne D. Cox, Channing J. Der
Summary: Mutation of CDKN2A and activation of KRAS are crucial for the development and malignant growth of PDAC. Combination treatment with CDK4/6 and ERK-MAPK inhibitors synergistically suppresses the growth of PDAC cells and organoids by blocking compensatory upregulation of signaling pathways. CRISPR-Cas9 screening and protein activity mapping uncover novel combinations that enhance the potency of CDK4/6 inhibitors and overcome drug-induced compensations in PDAC.
Article
Oncology
Raik Otto, Katharina M. Detjen, Pamela Riemer, Melanie Fattohi, Carsten Groetzinger, Guido Rindi, Bertram Wiedenmann, Christine Sers, Ulf Leser
Summary: Machine learning approaches can improve the classification accuracy of neuroendocrine neoplasms (NEN), but usually require large amounts of training data. This study used machine learning-aided classification based on the similarity between NEN and non-transformed pancreatic cell types to predict patient survival time, neoplastic grading, and carcinoma versus tumor subclassification, providing additional criteria for confident classification of ambiguous cases.
Article
Cell Biology
Thomas Sell, Christian Klotz, Matthias M. Fischer, Rosario Astaburuaga-Garcia, Susanne Krug, Jarno Drost, Hans Clevers, Christine Sers, Markus Morkel, Nils Bluethgen
Summary: Colorectal cancer progression is closely related to deregulation of intestinal differentiation trajectory. The sequential mutations of APC, KRAS, TP53, and SMAD4 enable oncogenic signaling and establish cancer hallmarks. Mass cytometry of isogenic human colon and patient-derived cancer organoids reveals a differentiation axis from normal to cancer states, shaped by the driver mutations. Cells along this axis can be influenced by subsequent mutations to promote or restrict stem cell properties. Nodes of the cancer cell signaling network remain coupled to the differentiation state. Single-cell RNA sequencing links protein signaling network to transcriptomic states with biological and clinical importance.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemical Research Methods
Alienke van Pijkeren, Anna-Sophia Egger, Madlen Hotze, Elisabeth Zimmermann, Tobias Kipura, Julia Grander, Andre Gollowitzer, Andreas Koeberle, Rainer Bischoff, Kathrin Thedieck, Marcel Kwiatkowski
Summary: Proteomics and metabolomics play vital roles in systems biology, and the simultaneous extraction of proteome and metabolome from the same sample is important. This study compared the performance of different surfactants and urea for proteome extraction and found that urea showed the best efficiency for simultaneous proteo-metabolome analysis.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Anna L. Fricke, Wignand W. D. Muhlhauser, Lena Reimann, Johannes P. Zimmermann, Christa Reichenbach, Bettina Knapp, Christian D. Peikert, Alexander M. Heberle, Erik Faessler, Sascha Schauble, Udo Hahn, Kathrin Thedieck, Gerald Radziwill, Bettina Warscheid
Summary: Phosphorylation-dependent signal transduction involving protein kinases AKT, S6K, and RSK plays a crucial role in regulating skeletal muscle cells. However, the specific targets of these kinases are still not completely understood. In this study, we used quantitative phosphoproteomics to investigate the effects of kinase inhibition in skeletal myotubes, revealing AKT-RAF cross talk, a negative feedback loop of RSK on ERK, and a potential connection between RSK and PI3K signaling. Our findings provide valuable insights into the kinase target landscape and the intricate signaling network involved in skeletal muscle regulation, including muscle development, integrity, and translation processes.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Oncology
Marie Solvay, Pauline Holfelder, Simon Klaessens, Luc Pilotte, Vincent Stroobant, Juliette Lamy, Stefan Naulaerts, Quentin Spillier, Raphael Frederick, Etienne De Plaen, Christine Sers, Christiane A. Opitz, Benoit J. van den Eynde, Jingjing Zhu
Summary: This study aimed to clarify the link between IDO1/TDO expression, AHR pathway activation, and immune suppression. The results showed that tryptophan deprivation sensitizes the AHR pathway, leading to increased Treg differentiation and immune suppression.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Medicine, General & Internal
Mihnea P. Dragomir, Teodor G. Calina, Eilis Perez, Simon Schallenberg, Meng Chen, Thomas Albrecht, Ines Koch, Peggy Wolkenstein, Benjamin Goeppert, Stephanie Roessler, George A. Calin, Christine Sers, David Horst, Florian Rossner, David Capper
Summary: This study developed a classifier that can differentiate between iCCAs, intrahepatic metastases of a PAAD, and normal bile duct tissue with high accuracy, which can improve the diagnosis of pancreato-biliary cancers of the liver.
Article
Medicine, General & Internal
Catriona Ling, Christian J. Versloot, Matilda E. Arvidsson Kvissberg, Guanlan Hu, Nathan Swain, Jose M. Horcas-Nieto, Emily Miraglia, Mehakpreet K. Thind, Amber Farooqui, Albert Gerding, Karen van Eunen, Mirjam H. Koster, Niels J. Kloosterhuis, Lijun Chi, YueYing ChenMi, Miriam Langelaar-Makkinje, Celine Bourdon, Jonathan Swann, Marieke Smit, Alain de Bruin, Sameh A. Youssef, Marjon Feenstra, Theo H. van Dijk, Kathrin Thedieck, Johan W. Jonker, Peter K. Kim, Barbara M. Bakker, Robert H. J. Bandsma
Summary: Severe malnutrition leads to structural and functional changes in the intestine, increasing the risk of infection and mortality. This study reveals the mechanisms underlying malnutrition enteropathy, including dysregulation of autophagy and mitochondrial homeostasis, as well as abnormalities in reactive oxygen species. The findings suggest that nicotinamide supplementation and modulation of SIRT1 and mTORC1 pathways may ameliorate malnutrition enteropathy in children with severe malnutrition.