Article
Cell & Tissue Engineering
Mohamed Elashry, Mebrie Kinde, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: In this study, it was found that exposure to hypoxia enhanced the proliferation and motility of skeletal muscle-derived stem cells, promoted the differentiation of muscle and adipocytes, and improved the efficiency of myoblast differentiation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Environmental Sciences
Yifan Pan, Hui Qin, Lu Zheng, Yong Guo, Wei Liu
Summary: HFPO-TA and HFPO-DA have been shown to adversely affect human bone marrow mesenchymal stem cells, with effects on cell cycle processes and promotion of cell proliferation. They also inhibit osteogenic differentiation and increase the expression of core pluripotency regulator NANOG.
ENVIRONMENTAL POLLUTION
(2022)
Article
Cell & Tissue Engineering
Marwan M. Merkhan, Matthew T. Shephard, Nicholas R. Forsyth
Summary: Modulation of environmental oxygen alters both the concentration and composition of the human mesenchymal stem cell (hMSC) secretome, which is likely to have significant implications for improving understanding and efficacy of hMSC-based therapeutics.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Anatomy & Morphology
Caiyun Ma, Yang Liu, Yingchun Ma, Lijie Jiang, Qianyi Huang, Gaofeng Liu, Yu Guo, Chunjing Wang, Changqing Liu
Summary: This study successfully isolated PMSCs from Sprague-Dawley rat fetal lung tissue for the first time and demonstrated their stemness and multi-differentiation potentials, suggesting a potential regenerative cell resource for cell regenerative therapy of lung injury.
Review
Engineering, Biomedical
Jay R. K. Samal, Vignesh K. Rangasami, Sumanta Samanta, Oommen P. Varghese, Oommen P. Oommen
Summary: Studies have shown that culturing MSCs in a low oxygen environment can upregulate stem cell markers and promote cell proliferation in an undifferentiated state. However, there is ambiguity in the results due to differences in oxygen gradients and culture conditions among different studies. This progress report focuses on conflicting findings related to the application of hypoxic conditions for improving MSC proliferation or differentiation, aiming to decipher the factors influencing MSC characteristics under hypoxia.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Agnese Gugliandolo, Emanuela Mazzon
Summary: Dental-derived mesenchymal stem cells have a prominent neuroregenerative potential and their secretome, consisting of bioactive molecules, can effectively promote neurite outgrowth and neuroprotective effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Francesca Della Sala, Gennaro Longobardo, Gianluca Lista, Francesco Messina, Assunta Borzacchiello
Summary: Pharmacological therapies can reduce symptomatology of lung injury, but tissue damage restoration is still lacking. MSCs have the potential to secrete factors that regulate permeability, decrease inflammation, enhance repair, and inhibit bacterial growth. HA promotes MSCs differentiation into ATII cells. The combination of HA and secretome can enhance MSCs differentiation, improve cell viability and migration, and exhibit anti-inflammatory properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
Summary: This study demonstrates the successful generation of AGM-like hematopoietic system from human pluripotent stem cells through temporal manipulation of Wnt signaling. The generated hematopoietic progenitor cells resemble primary cord blood HSCs at the transcriptional level and exhibit lymphoid and myeloid potential in vitro. The cells also show the ability to home to a definitive hematopoietic site, rescue bloodless zebrafish, and engraft and repopulate in mouse recipients.
Article
Immunology
Yanran Zhao, Curtis Cai, Jerome Samir, Jean-Louis Palgen, Elizabeth Keoshkerian, Hui Li, Rowena A. Bull, Fabio Luciani, Hongyan An, Andrew R. Lloyd
Summary: The study identified CD8 T-SCM subsets in healthy individuals exposed to CMV or Influenza virus, with the T(SCM)CD122(hi) expressing subset showing greater proliferation, multipotency, and polyfunctionality. The findings suggest that the T(SCM)CD122(hi) phenotype may be preferred for immune induction and adoptive immunotherapy due to its activated memory-cell like transcriptional profile.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Michela Mocchi, Elia Bari, Giorgio Marrubini, Andrea Foglio Bonda, Sara Perteghella, Fulvio Tartara, Fabio Cofano, Giuseppe di Perna, Lorella Giovannelli, Delia Mandracchia, Marzio Sorlini, Diego Garbossa, Maria Luisa Torre, Lorena Segale
Summary: This study aimed to optimize the production process of mesenchymal stem cell-derived secretome, lyosecretome, by investigating sources of variability and modifying the manufacturing process. Different excipient ratios did not significantly affect the product quality, but increasing the total concentration of excipients improved the preservation of proteins and lipids. By concentrating the final product to achieve a specific protein concentration, the manufacturing process was standardized, resulting in a more reproducible composition and higher biological activity of the product.
Review
Biochemistry & Molecular Biology
Batoul Chouaib, Mandana Haack-Sorensen, Franck Chaubron, Frederic Cuisinier, Pierre-Yves Collart-Dutilleul
Summary: Mesenchymal stem cell secretome or conditioned medium (MSC-CM) is a crucial starting point for various derived products and can be used for regenerative medicine. However, issues such as manufacturing protocols and quality control must be addressed before their clinical application. This review highlights the importance of standardized procedures in conditioned medium production and the influence of various factors on the quality of the secretome and its derivatives.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Roberta Squecco, Alessia Tani, Flaminia Chellini, Rachele Garella, Eglantina Idrizaj, Irene Rosa, Sandra Zecchi-Orlandini, Mirko Manetti, Chiara Sassoli
Summary: This study demonstrates that bone marrow-mesenchymal stem/stromal cells (MSCs) secretome has positive effects on skeletal muscle repair, attenuating tissue damage and protecting muscle fibers from apoptosis. Additionally, MSC secretome affects key cells involved in muscle regeneration, contributing to the replenishment of muscle satellite cells and functional modifications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Rebecca M. Harman, Charlotte Marx, Gerlinde R. van de Walle
Summary: The therapeutic potential of MSC secretome is being intensely studied, but its complexity and variability pose challenges for its application in human medicine. A deeper understanding of the factors that make up the secretome and the ability to manipulate MSCs to consistently secrete factors of biologic importance will improve MSC therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Aliosha I. Figueroa-Valdes, Catalina de la Fuente, Yessia Hidalgo, Ana Maria Vega-Letter, Rafael Tapia-Limonchi, Maroun Khoury, Francisca Alcayaga-Miranda
Summary: Cell therapy is shifting towards cell-free treatments using small extracellular vesicles (sEV) that mimic the effects of parental cells, but faces challenges in large-scale production and regulation compliance. Using a regulatory-compliant medium like Oxium(TM)EXO can significantly increase sEV secretion rates and improve production efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Richard Biener, Thomas Horn, Alexander Komitakis, Ines Schendel, Leon Koenig, Anna Hauenstein, Alina Ludl, Andrea Speidel, Svenja Schmid, Julian Weisser, Max Brossmann, Sofia Kern, Max Kronmueller, Sonja Vierkorn, Lennart Suckow, Arthur Braun
Summary: V. natriegens is a halophilic bacterium with the fastest generation time reported so far. A low-chloride chemically defined medium has been developed to achieve high-cell-density cultivation and prevent corrosion on metal surfaces.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Oncology
Kyungsoo Jung, Joon-Seok Choi, Beom-Mo Koo, Yu Jin Kim, Ji-Young Song, Minjung Sung, Eun Sol Chang, Ka-Won Noh, Sungbin An, Mi-Sook Lee, Kyoung Song, Hannah Lee, Ryong Nam Kim, Young Kee Shin, Doo-Yi Oh, Yoon-La Choi
Summary: This study explored new biomarkers using outlier analysis and identified two genes (TM4SF4 and LRRK2) as potential targets for lung and breast cancer. The knockdown of these genes suppressed cancer cell growth and LRRK2-overexpressing cell lines showed increased sensitivity to LRRK2-IN-1, indicating their therapeutic potential.
CANCER RESEARCH AND TREATMENT
(2021)
Article
Medicine, Research & Experimental
Jaebeom Cho, Hye-Young Min, Ho Jin Lee, Seung Yeob Hyun, Jeong Yeon Sim, Myungkyung Noh, Su Jung Hwang, Shin-Hyung Park, Hye-Jin Boo, Hyo-Jong Lee, Sungyoul Hong, Rang-Woon Park, Young Kee Shin, Mien-Chie Hung, Ho-Young Lee
Summary: Slow-cycling/dormant cancer cells play crucial roles in cancer relapse and drug resistance. A specific subpopulation of SCCs in NSCLC has been identified, showing dormancy-like phenotypes and high survival capacity through upregulation of RGS2. RGS2 induces translational arrest in SCCs via eIF2 alpha phosphorylation, which can be reversed by RGS2 antagonism or phosphodiesterase S inhibitors, leading to apoptosis. Combining low-dose chemotherapy with translation-promoting pharmacological intervention is an effective strategy to prevent tumor progression in NSCLC patients after rigorous chemotherapy.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Multidisciplinary Sciences
Hannah Lee, Mi Jeong Kwon, Beom-Mo Koo, Hee Geon Park, Jinil Han, Young Kee Shin
Summary: Using public gene expression microarray datasets, researchers classified breast cancer patients into different risk groups based on clinical and proliferation risk, and developed an immune prognostic index for predicting recurrence risk. This index was found to be an independent prognostic factor for disease-free survival or distant metastasis-free survival and outperformed clinicopathological variables in predicting prognosis. The immune prognostic index can identify low-risk patients among those classified as clinically and proliferation high-risk, regardless of the molecular subtype of breast cancer, potentially overcoming limitations of current multigene assays.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Jin-Ock Kim, Kwang-Hyeok Kim, Eun Ji Baek, Bomi Park, Min Kyung So, Byoung Joon Ko, Han-Jik Ko, Sang Gyu Park
Summary: The study developed an anti-c-Kit antibody-drug conjugate, NN2101-DM1, which showed potent growth-inhibitory activities against c-Kit-positive cancer cell lines, especially in cases of TKI resistance. In mouse xenograft models, NN2101-DM1 exhibited strong growth-inhibitory activities against imatinib-resistant GIST and SM cells, and a significantly higher anti-cancer effect than carboplatin/etoposide against SCLC cells. Combination of NN2101-DM1 with imatinib in imatinib-sensitive GIST cells induced complete remission in mouse xenograft models, suggesting its potential as a therapeutic agent for wild-type and mutant c-Kit-positive cancers.
MOLECULAR ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Songyi Seo, Koung Li Kim, Yeongju Yeo, Ryul- Kim, Hayoung Jeong, Jin-Ock Kim, Sun-Hwa Song, Mi-Jin An, Jung-Woong Kim, Hye Kyoung Hong, Min Hee Ham, Se Joon Woo, Jong-Hyuk Sung, Sang Gyu Park, Wonhee Suh
Summary: The study demonstrated that the fully human monoclonal antibody NN2101 can effectively treat neovascular AMD by inhibiting the SCF/cKIT signaling pathway, with efficacy comparable to the VEGF inhibitor aflibercept. Combined use of NN2101 and aflibercept showed additive therapeutic effects on CNV, without causing ocular toxicity or interfering with early retinal vascular development in mice. Ocular pharmacokinetic analysis in rabbits confirmed NN2101's suitability for intravitreal injection.
Article
Biotechnology & Applied Microbiology
Na Young Kim, Won Rak Son, Min Hoon Lee, Hong Seok Choi, Jun Young Choi, Young Jo Song, Chi Ho Yu, Dong Hyun Song, Gyeung Haeng Hur, Seong Tae Jeong, Sung Youl Hong, Young Kee Shin, Sungho Shin
Summary: This study developed a synthetic dual-pathogen DNA vaccine for anthrax and botulism, which elicited high levels of antibody response and provided over 50% protection against both pathogens. The study suggests a prospective approach for developing emergency vaccines that can be distributed on a massive scale in response to biothreat emergencies or infectious disease outbreaks.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Dermatology
Advaitaa Ravipati, Sabrina Nolan, Martin Alphonse, Dustin Dikeman, Christine Youn, Yu Wang, Nicholas Orlando, Garrett Patrick, Steven Lee, Roger Ortines, Haiyun Liu, Robert J. Miller, Carly A. Dillen, Mark Marchitto, S. Sarah Cai, Lloyd S. Miller, Nathan K. Archer
Summary: Signal transducer and activator of transcription 3 (STAT3) plays an important role in the pathogenesis of psoriasis. This study found that STAT3 signaling in keratinocytes is more important than in T cells for the development of psoriasis-like dermatitis. The mechanism involves upstream IL-6R signaling in keratinocytes and downstream inhibition of type 1 immunity-associated CXCL10 responses.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Medicine, Research & Experimental
Shin Ung Kang, Soo Youn Cho, Hyojin Jeong, Jinil Han, Ha Yeong Chae, Hobin Yang, Chang Ohk Sung, Yoon-La Choi, Young Kee Shin, Mi Jeong Kwon
Summary: The study demonstrated that MMP11 expression in mononuclear inflammatory cells, predominantly macrophages, is an independent negative prognostic factor in breast cancer. Furthermore, MMP11-overexpressing macrophages promote migration of HER2-positive breast cancer cells and monocyte recruitment through CCL2-CCR2 signaling, suggesting a pro-tumoral role of MMP11 in macrophages in HER2-positive breast cancer through interaction with cancer cells and other cells in the tumor microenvironment.
LABORATORY INVESTIGATION
(2022)
Article
Anesthesiology
Hyesu Kim, Hyungsup Kim, Hawon Cho, Byeongjun Lee, Huan-Jun Lu, Kyungmin Kim, Sooyoung Chung, Won-Sik Shim, Young Kee Shin, Xinzhong Dong, John N. Wood, Uhtaek Oh
Summary: Research has found that ANO1 is a transduction channel mediating Mrgpr-dependent itch signals. Genetic ablation of ANO1 in dorsal root ganglion neurons significantly reduces scratching behaviors in response to acute and chronic Mrgpr-dependent itch models and epidermal hyperplasia induced by dry skin. Overexpression of ANO1 in ANO1-deficient mice rescues the impaired itching observed in these mice.
Article
Chemistry, Medicinal
Chaithanya Chelakkot, Hobin Yang, Young Kee Shin
Summary: Shedding cancer cells from the primary site or bone marrow into the circulatory system and their subsequent formation of secondary metastatic lesions is a key feature of invasive cancers. Circulating tumor cells (CTCs), found either as single cells or clusters, carry valuable information and have the potential to predict cancer prognosis, progression, and guide tailored therapies.
Article
Biochemistry & Molecular Biology
Kwang-Hyeok Kim, Jin-Ock Kim, Jeong-Yang Park, Min-Duk Seo, Sang Gyu Park
Summary: Lung cancer is a major cause of cancer-related deaths, with small cell lung cancer (SCLC) accounting for a significant portion. Overexpression of c-Kit is a characteristic of SCLC. This study found that the antibody-drug conjugate 4C9-DM1 shows potential as a therapeutic agent for SCLC, as it efficiently induces apoptosis and inhibits tumor growth. Combination treatment with other drugs further enhances its anti-tumor effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Xintong Dong, Nathachit Limjunyawong, Elizabeth Sypek, Gaofeng Wang, Roger Ortines, Christine Youn, Martin P. Alphonse, Dustin Dikeman, Yu Wang, Mark Lay, Ruchita Kothari, Chirag Vasavda, Priyanka Pundir, Loyal Goff, Lloyd S. Miller, Wuyuan Lu, Luis A. Garza, Brian S. Kim, Nathan K. Archer, Xinzhong Dong
Summary: The signaling pathway between epithelial cells and neutrophils is crucial for maintaining healthy skin ecology and promoting antibacterial host defense. Defensins and Mrgpra2 play critical roles in combating infections and maintaining microbial homeostasis.
Article
Dermatology
Christine Youn, Dustin A. Dikeman, Evelyn Chang, Haiyun Liu, Sabrina J. Nolan, Martin P. Alphonse, Daniel P. Joyce, Qi Liu, James Meixiong, Xinzhong Dong, Lloyd S. Miller, Nathan K. Archer
Summary: Phosphodiesterase 4 (PDE4) is highly expressed in keratinocytes and immune cells, promoting pro-inflammatory responses. PDE4 inhibitors such as crisaborole have been approved for the treatment of inflammatory skin diseases, but their immunopathogenetic effects are not fully understood.
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Chaithanya Chelakkot, Vipin Shankar Chelakkot, Youngkee Shin, Kyoung Song
Summary: Cancer cells undergo metabolic reprogramming to adopt a glycolysis-dominant metabolic profile, providing them with a survival advantage and contributing to a pro-cancerous tumor environment. This metabolic switch also leads to increased resistance to chemotherapy and radiation. Immune cells in the tumor microenvironment also exhibit a similar metabolic shift, leading to competition with cancer cells for nutrients. Targeting enhanced glycolysis in cancer cells has shown promise in improving treatment efficacy, but effective strategies are still lacking. This review highlights the importance of metabolic reprogramming in cancer cells and the potential of targeting it to enhance conventional cancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kyoung Song, Hun Seok Lee, Lina Jia, Chaithanya Chelakkot, Nirmal Rajasekaran, Young Kee Shin
Summary: SMAD4 is a critical signaling mediator that regulates various cellular functions. This study confirms the interaction between SMAD4 and MMAB and reveals that SMAD4 controls cancer cell metabolism by regulating MMAB expression. Knockdown of MMAB affects cancer cell metabolism and induces apoptosis.
MOLECULES AND CELLS
(2022)
