Article
Chemistry, Applied
Jiawen Feng, Han Tian, Xu Chen, Xixi Cai, Xiaodan Shi, Shaoyun Wang
Summary: This article investigates the interactions between fish gelatin and Tremella polysaccharides using turbidity titration. The results show that the turbidity is influenced by pH, polymer ratio, total polymer concentration, and ionic strength. The complex coacervates formed at a mass ratio of 1:1 and pH 4.0 exhibit a porous network structure and have the strongest viscosity at pH 3.5.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Applied
Wenfei Xiong, Ya Li, Cong Ren, Jing Li, Bin Li, Fang Geng
Summary: Investigating the thermodynamic parameters in the formation process of protein-polysaccharide complex coacervates is crucial for understanding the binding mechanism. pH, salt ion strength, and temperature were found to influence the thermodynamic parameters of gelatin (G)-pectin (P) complex coacervation. The binding between G and P involves electrostatic bonding, hydrogen bonding, and hydrophobic interactions.
FOOD HYDROCOLLOIDS
(2021)
Article
Food Science & Technology
Chen Cheng, Zongcai Tu, Hui Wang
Summary: The aim of this study was to investigate the phase behavior and structural properties of fish gelatin complex coacervation with carboxylated chitosan. Zeta potential indicated that electrostatic interaction drove the complex coalescence of fish gelatin and carboxylated chitosan. The study found that the addition of carboxylated chitosan changed the tertiary conformation of fish gelatin and resulted in a shift in its structure.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Materials Science, Multidisciplinary
Juanfeng Sun, Jessica D. Schiffman, Sarah L. Perry
Summary: This paper investigates the effect of adding small amounts of methanol or ethanol to complex coacervates of chitosan and hyaluronic acid, and explores the impact of cosolvent addition on the phase behavior and rheological properties of the resulting coacervates. Moreover, the potential of using different superposition techniques to study the rheology of coacervates is proposed.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Applied
Yunshan Liu, Jianzhong Jiang
Summary: In this study, /9-ionone was encapsulated using complex coacervation of gelatin and pectin, followed by cross-linking with glutaraldehyde. The shape, size, and encapsulation efficiency of the microcapsule were significantly affected by the gelatin/pectin ratio and pH value. The microcapsules exhibited good thermal stability and slow release performance, making them a potential carrier for flavors like /9-ionone.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Physical
Weigang Zhong, Tiehua Zhang, Chao Dong, Jiatong Li, Jingwen Dai, Cuina Wang
Summary: The study investigated the effects of ionic strength on the formation and properties of WPI and HA complexes, showing that NaCl concentration of 5-40 mM had the most significant enhancement on the complexation. The presence of NaCl affected pH values, surface charge, conformation, and fluorescence intensity of the complexes. The critical NaCl concentration for optimal complexation was around 40 mM, indicating potential applications of WPI/HA complexes as delivery vehicles for bioactive substances.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Applied
Diewei Li, Heping Cui, Khizar Hayat, Xiaoming Zhang, Chi-Tang Ho
Summary: Chitosan was applied to improve the stability and resistance against harsh environment of gelatin/CMC coacervates. Microcapsules were prepared with eugenol as the core material, and chitosan coating helped maintain eugenol retention at 70%-80% in harsh environments.
FOOD HYDROCOLLOIDS
(2022)
Article
Biochemistry & Molecular Biology
Ying Chen, Weifei Wang, Weiqian Zhang, Dongming Lan, Yonghua Wang
Summary: This study constructed three probiotic microcapsule models using gelatin-gum arabic complex coacervate as wall material. The results showed that microcapsules containing diacylglycerol (DAG) oil and probiotics (GE-D-GA) improved cell membrane fluidity, maintained protein and nucleic acid stability, and decreased cell membrane damage. GE-D-GA exhibited the highest freeze-dried survival rate, best cell viability retention under thermotolerance or storage, and provided the best protection for probiotics under simulated gastrointestinal conditions. Co-encapsulation of DAG oil and probiotics is a promising strategy to resist adverse conditions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Jiaying Zhang, Guoliang Jia, Zhao Wanbin, Jiang Minghao, Yulong Wei, Jingyi Hao, Xiaolin Liu, Zhilin Gan, Aidong Sun
Summary: Nanocapsules for zeaxanthin encapsulation were developed through complex coacervation of gelatin and sodium carboxymethyl cellulose. The optimal G-CMC mass mixing ratio was found to be 9:1 with a pH of 4.50. FTIR analysis confirmed the electrostatic interaction in the formation of G-CMC complex coacervates, while thermal gravimetric analysis showed enhanced thermal stability of zeaxanthin through nanoencapsulation.
FOOD HYDROCOLLOIDS
(2021)
Article
Biochemistry & Molecular Biology
A. Basak Kayitmazer, Fatih Comert, Henning H. Winter, Phillip B. Messersmith
Summary: Rheological experiments showed that the linear viscoelasticity of HA/CHI coacervates depends on the molecular weight of the polymers. Catechol-conjugated HA and CHI exhibited a significant increase in moduli, suggesting their potential as injectable tissue scaffolds or medical adhesives.
Article
Chemistry, Applied
Lili Tian, Yrjo H. Roos, Song Miao
Summary: Different methods were used to monitor transformations and protein-polysaccharide complexes formation resulting from the interaction of whey protein isolate (WPI) and Tremella fuciformis polysaccharide (TPS) over the pH range of 2.0-7.0. The WPI:TPS ratio and biopolymer concentration altered phase boundaries and critical pH values.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Applied
Martin Daniel Aya Rodriguez, Danilo C. Vidotto, Ana Augusta O. Xavier, Raphaela A. Mantovani, Guilherme M. Tavares
Summary: Heteroprotein complex coacervation is a phenomenon triggered by the interaction between oppositely charged proteins, resulting in two liquid phases in equilibrium. In this study, the complex coacervation between type A gelatin (GA) and native and thermal treated 8-lactoglobulin (8-lg and 8-lg (TT)) was investigated. The thermal treatment led to changes in the surface properties of the proteins, but had minor effects on their aggregation state and lutein binding ability. The optimal condition for the complex coacervation was determined, and the obtained coacervates showed high lutein entrapment efficiency.
FOOD HYDROCOLLOIDS
(2023)
Article
Agriculture, Multidisciplinary
Faezeh Ardestani, Ali Haghighi Asl, Ali Rafe
Summary: The research found that the optimal pH for coacervation between NaCas and HMP is at pH 3.30, where the complex shows the highest turbidity, particle size, and viscosity. The best coacervation condition was achieved at a ratio of 4:1 and a total biopolymer concentration of 0.4% w/v. Increasing the total concentration of biopolymers shifted the NaCas/pectin ratio to higher pH values. The maximum coacervate yield was achieved at 39.8% w/w with a ratio of 4:1 of NaCas/HMP and a total biopolymer concentration of 0.4% w/v.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2022)
Article
Food Science & Technology
Qi Guo, Shuai Li, Gengan Du, Hong Chen, Xiaohai Yan, Shuaidan Chang, Tianli Yue, Yahong Yuan
Summary: Carvacrol-loaded microcapsules were successfully developed through coacervation complexes of whey protein isolate (WPI) and high methoxyl pectin (HMP), followed by cross-linking with tannic acid (TA), showing improved stability and antioxidant properties.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yongchao Jiang, Yingying Guo, Haonan Wang, Xiaofeng Wang, Qian Li
Summary: A double-layered hydrogel coating with spatiotemporal drug release capacity was applied on a poly-caprolactone (PCL) fibrous scaffold to promote quick endothelialization on vascular grafts. The composite coating consisted of dopamine-modified hyaluronic acid (HA) hydrogel and gelatin hydrogel, with heparin and chondroitin sulfate introduced for drug release behavior. The coating improved the surface properties of the PCL scaffold and enhanced endothelial cell proliferation and angiogenesis behaviors. In vitro tests confirmed the long-term anticoagulation property of the modified scaffold. This research provides a universal strategy for promoting endothelialization and anticoagulation in vascular grafts.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Md Abdur Razzak, Moojoong Kim, Donghwa Chung
CARBOHYDRATE POLYMERS
(2016)
Article
Chemistry, Applied
Md Abdur Razzak, Ji Eun Lee, Shin Sik Choi
FOOD HYDROCOLLOIDS
(2019)
Article
Biochemical Research Methods
Arvie Camille de Guzman, Md Abdur Razzak, Batmagnai Purevdulam, Shin Sik Choi
BIOTECHNOLOGY JOURNAL
(2020)
Article
Chemistry, Applied
Md. Abdur Razzak, Shin Sik Choi
Summary: The interaction between ovalbumin (OVA) and isoflavonoid glabridin (GB) was studied through spectroscopic and molecular docking techniques. GB was found to bind to OVA mainly through hydrogen bonding and hydrophobic forces, leading to changes in OVA's secondary structure. Molecular docking simulations supported the experimental findings. Additionally, the GB-OVA complex increased the aqueous solubility of GB.
Article
Food Science & Technology
Md Abdur Razzak, Jiaoyang Li, Shin Sik Choi
Summary: This study investigated the interaction between curcumin and ovalbumin, revealing a stable complex formation with maintained physicochemical properties of curcumin. The complexation with ovalbumin significantly enhanced the aqueous solubility of curcumin and reduced its degradation in aqueous solutions over a wide range of pH. The study provides insights into the binding mechanisms of small chemicals and large biomolecules for medicinal and functional food applications.
Article
Biotechnology & Applied Microbiology
Jiaoyang Li, Hui Jin, Md Abdur Razzak, Eun Ji Kim, Shin Sik Choi
Summary: A pH-responsive nano-sized complex consisting of chitosan and alginate was developed as an oral vehicle for protein-based drugs. The complex showed inhibition of protein release in an acidic environment and bursting release in a neutral environment, indicating its potential for oral delivery of protein-based drugs or vaccines.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Vikash Chandra Roy, Md. Abdur Razzak, Truc Cong Ho, Duraiarasan Surendhiran, Jin-Seok Park, Byung-Soo Chun
Summary: The present work focuses on the preparation and stabilization of quercetin-loaded zein colloidal nanoparticles using κ-carrageenan as a stabilizer. The newly prepared zein-KC complex colloidal particles loaded with quercetin showed spherical shapes, small particle sizes, high negative zeta potential, and good encapsulation efficiency. The interactions among zein, quercetin, and κ-carrageenan were confirmed, and the KC-coated zein colloidal particles exhibited improved physicochemical properties and antioxidant activity of the encapsulated quercetin.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Arvie Camille V. de Guzman, Md. Abdur Razzak, Joong Hee Cho, Ji Yi Kim, Shin Sik Choi
Summary: This study fabricated curcumin nanoparticles using human serum albumin as a carrier and found that these nanoparticles could ameliorate Parkinson's disease features in a C. elegans model.
Article
Chemistry, Applied
Md. Abdur Razzak, Min-Soo Jeong, Min Jeong Kim, Seong-Jun Cho
Summary: In this study, the associative phase behavior of cricket protein isolate (CPI) and sodium alginate (AL) in aqueous solutions was investigated. The formation of soluble or insoluble complexes between the two biopolymers was found to be influenced by factors such as pH, biopolymer ratio, total biopolymer concentration, and ionic strength. Ionic interactions were identified as the main driving force for the formation of insoluble complexes, while hydrogen bonding and hydrophobic interactions also played significant roles.
Article
Chemistry, Applied
Md Abdur Razzak, Seong-Jun Cho
Summary: This study investigated the interaction between capsaicin and ovalbumin (OVA) and casein (CAS) at pH 7.4, and explored the potential of using these food proteins as carriers for capsaicin. The findings showed that capsaicin could bind with OVA/CAS, leading to increased fluorescence intensity and specific protein conformational changes. The results also demonstrated a high encapsulation efficiency of capsaicin with OVA/CAS, enhancing their antioxidant activity synergistically.
FOOD HYDROCOLLOIDS
(2022)
Article
Nanoscience & Nanotechnology
Hae-Ji Kang, Jiaoyang Li, Md Abdur Razzak, Gi-Deok Eom, Keon-Woong Yoon, Jie Mao, Ki-Back Chu, Hui Jin, Shin Sik Choi, Fu-Shi Quan
Summary: Lessons from the recent COVID-19 pandemic highlight the importance of rapidly developing and administering an effective vaccine. In this study, a chitosan-alginate complex carrier system was designed to encapsulate an inactivated influenza virus vaccine and evaluated for oral administration in mice. The nanocomposite vaccines demonstrated successful delivery of the vaccine, induction of virus-specific antibodies, and enhanced T-cell responses. These results suggest that chitosan-alginate polymeric nanocomposites hold promise as delivery systems for oral influenza vaccines.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Physical
Md. Abdur Razzak, Seong-Jun Cho
Summary: As people's awareness of the health benefits of foods and their nutritional benefits for preventing diseases and promoting health increases, the demand for functional foods rich in proteins, fiber, and bioactives like capsaicin (CAP) is constantly rising. This study demonstrated that the electrostatic complexes developed by cricket protein isolate (CPI) and alginate (AL) can effectively encapsulate CAP, improving its solubility and protecting it at acidic pHs. The findings contribute to the growing body of knowledge that validates protein-polysaccharide complexation as a promising strategy for developing edible delivery systems.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Md. Abdur Razzak, Ji Eun Lee, Hee Ho Park, Tai Hyun Park, Shin Sik Choi
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2018)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
