TALENs and CRISPR/Cas9 fuel genetically engineered clinically relevantXenopus tropicalistumor models

Insight from Frogs: Sonic Hedgehog Gene Expression and a Re-evaluation of the Vertebrate Odontogenic Band

(2016) Theresa M. Grieco et al.   Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology

Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes

(2016) Alexa Burger et al.   DEVELOPMENT

Echoes of the embryo: using the developmental biology toolkit to study cancer

(2016) Nicole M. Aiello et al.   Disease Models & Mechanisms

Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders

(2016) Mitsuki Shigeta et al.   GENES TO CELLS

Cell-free Xenopus egg extracts for studying DNA damage response pathways

(2016) Steven Cupello et al.   INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY

CRISPR: gene editing is just the beginning

(2016) Heidi Ledford   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos

(2016) Zhongzhen Liu et al.   Cell and Bioscience

c21orf59/kurly Controls Both Cilia Motility and Polarization

(2016) Kimberly M. Jaffe et al.   Cell Reports

BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment

(2016) Annekatrien Boel et al.   Scientific Reports

CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis

(2016) Thomas Naert et al.   Scientific Reports

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

An oncologist׳s friend: How Xenopus contributes to cancer research

(2015) Laura J.A. Hardwick et al.   DEVELOPMENTAL BIOLOGY

Semi-solid tumor model in Xenopus laevis/gilli cloned tadpoles for intravital study of neovascularization, immune cells and melanophore infiltration

(2015) Nikesha Haynes-Gimore et al.   DEVELOPMENTAL BIOLOGY

CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus

(2015) Dipankan Bhattacharya et al.   DEVELOPMENTAL BIOLOGY

Heritable CRISPR/Cas9-mediated targeted integration in Xenopus tropicalis

(2015) Zhaoying Shi et al.   FASEB JOURNAL

Passenger Mutations Confound Interpretation of All Genetically Modified Congenic Mice

(2015) Tom Vanden Berghe et al.   IMMUNITY

Co-deleting Pten with Rb in retinal progenitor cells in mice results in fully penetrant bilateral retinoblastomas

(2015) Chencheng Xie et al.   Molecular Cancer

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

(2015) Lauren R Polstein et al.   Nature Chemical Biology

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling

(2015) Marc Zuckermann et al.   Nature Communications

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

(2015) Manuel Stemmer et al.   PLoS One

Engineering Xenopus embryos for phenotypic drug discovery screening

(2014) Stefan M. Schmitt et al.   ADVANCED DRUG DELIVERY REVIEWS

Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis

(2014) X. Guo et al.   DEVELOPMENT

In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system

(2014) Danilo Maddalo et al.   NATURE

E-CRISP: fast CRISPR target site identification

(2014) Florian Heigwer et al.   NATURE METHODS

Optimized cell transplantation using adult rag2 mutant zebrafish

(2014) Qin Tang et al.   NATURE METHODS

An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage

(2014) Eli J. Fine et al.   NUCLEIC ACIDS RESEARCH

SAPTA: a new design tool for improving TALE nuclease activity

(2014) Yanni Lin et al.   NUCLEIC ACIDS RESEARCH

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

(2014) Tessa G. Montague et al.   NUCLEIC ACIDS RESEARCH

Mojo Hand, a TALEN design tool for genome editing applications

(2013) Kevin L Neff et al.   BMC BIOINFORMATICS

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays

(2013) Satoshi Ota et al.   GENES TO CELLS

Biallelic genome modification in F0Xenopus tropicalisembryos using the CRISPR/Cas system

(2013) Ira L. Blitz et al.   GENESIS

Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis inXenopus tropicalis

(2013) Takuya Nakayama et al.   GENESIS

The zebrafish reference genome sequence and its relationship to the human genome

(2013) Kerstin Howe et al.   NATURE

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

E-TALEN: a web tool to design TALENs for genome engineering

(2013) Florian Heigwer et al.   NUCLEIC ACIDS RESEARCH

An implantable MEMS micropump system for drug delivery in small animals

(2012) Heidi Gensler et al.   BIOMEDICAL MICRODEVICES

Neural crest delamination and migration: From epithelium-to-mesenchyme transition to collective cell migration

(2012) Eric Theveneau et al.   DEVELOPMENTAL BIOLOGY

Efficient Detection, Quantification and Enrichment of Subtle Allelic Alterations

(2012) J. Chen et al.   DNA RESEARCH

Xenopus: An ideal system for chemical genetics

(2012) Grant N. Wheeler et al.   GENESIS

The Hitchhiker's guide toXenopusgenetics

(2012) Anita Abu-Daya et al.   GENESIS

The complex world of WNT receptor signalling

(2012) Christof Niehrs   NATURE REVIEWS MOLECULAR CELL BIOLOGY

TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction

(2012) Erin L. Doyle et al.   NUCLEIC ACIDS RESEARCH

Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs)

(2012) Y. Lei et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Coexpression of Normally Incompatible Developmental Pathways in Retinoblastoma Genesis

(2011) Justina McEvoy et al.   CANCER CELL

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Directed Evolution of an Enhanced and Highly Efficient FokI Cleavage Domain for Zinc Finger Nucleases

(2010) Jing Guo et al.   JOURNAL OF MOLECULAR BIOLOGY

Creating Designed Zinc-Finger Nucleases with Minimal Cytotoxicity

(2010) Sivaprakash Ramalingam et al.   JOURNAL OF MOLECULAR BIOLOGY

Rb regulates fate choice and lineage commitment in vivo

(2010) Eliezer Calo et al.   NATURE

Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures

(2010) Yannick Doyon et al.   NATURE METHODS

The Genome of the Western Clawed Frog Xenopus tropicalis

(2010) U. Hellsten et al.   SCIENCE

An in vivo chemical library screen in Xenopus tadpoles reveals novel pathways involved in angiogenesis and lymphangiogenesis

(2009) R. E. Kalin et al.   BLOOD

Molecular basis of morphogenesis during vertebrate gastrulation

(2009) Yingqun Wang et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Tumorigenesis and anti-tumor immune responses in Xenopus

(2009) Ana Goyos   Frontiers in Bioscience-Landmark

Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease

(2009) Hervé Acloque et al.   JOURNAL OF CLINICAL INVESTIGATION

Sonic Hedgehog and Bone Morphogenetic Protein-4 Signaling Pathway Involved in Epithelial Cell Renewal along the Radial Axis of the Intestine

(2008) Atsuko Ishizuya-Oka et al.   DIGESTION

Mouse xenograft models vs GEM models for human cancer therapeutics

(2008) A. Richmond et al.   Disease Models & Mechanisms

The Xenopus Cell Cycle: An Overview

(2008) Anna Philpott et al.   MOLECULAR BIOTECHNOLOGY