Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders

Digital detection of endonuclease mediated gene disruption in the HIV provirus

(2016) Ruth Hall Sedlak et al.   Scientific Reports

CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus

(2015) Dipankan Bhattacharya et al.   DEVELOPMENTAL BIOLOGY

Xenbase: Core features, data acquisition, and data processing

(2015) Christina James-Zorn et al.   GENESIS

High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes

(2015) A. Paix et al.   GENETICS

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9

(2015) Gaurav K. Varshney et al.   GENOME RESEARCH

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

(2015) Daesik Kim et al.   NATURE METHODS

The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos

(2015) Kei Miyamoto et al.   PLoS One

Genome Engineering with CRISPR-Cas9 in the Mosquito Aedes aegypti

(2015) Kathryn E. Kistler et al.   Cell Reports

Comparison of T7E1 and Surveyor Mismatch Cleavage Assays to Detect Mutations Triggered by Engineered Nucleases

(2015) Léna Vouillot et al.   G3-Genes Genomes Genetics

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish

(2015) Hirohito Kotani et al.   PLoS One

Engineering Xenopus embryos for phenotypic drug discovery screening

(2014) Stefan M. Schmitt et al.   ADVANCED DRUG DELIVERY REVIEWS

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing

(2014) Charles C Bell et al.   BMC GENOMICS

Making designer mutants in model organisms

(2014) Y. Peng et al.   DEVELOPMENT

Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis

(2014) X. Guo et al.   DEVELOPMENT

Multiple genome modifications by the CRISPR/Cas9 system in zebrafish

(2014) Satoshi Ota et al.   GENES TO CELLS

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

(2014) Cem Kuscu et al.   NATURE BIOTECHNOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Microhomology-based choice of Cas9 nuclease target sites

(2014) Sangsu Bae et al.   NATURE METHODS

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

An Efficient Genotyping Method for Genome-modified Animals and Human Cells Generated with CRISPR/Cas9 System

(2014) Xiaoxiao Zhu et al.   Scientific Reports

RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein

(2014) Jeong-Soo Lee et al.   G3-Genes Genomes Genetics

Targeted mutagenesis using CRISPR/Cas system in medaka

(2014) S. Ansai et al.   Biology Open

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays

(2013) Satoshi Ota et al.   GENES TO CELLS

Biallelic genome modification in F0Xenopus tropicalisembryos using the CRISPR/Cas system

(2013) Ira L. Blitz et al.   GENESIS

Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis inXenopus tropicalis

(2013) Takuya Nakayama et al.   GENESIS

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

(2013) Y. H. Sung et al.   GENOME RESEARCH

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing

(2013) Nicola Crosetto et al.   NATURE METHODS

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

(2013) K.-i. T. Suzuki et al.   Biology Open

Xenopuswhite papers and resources: Folding functional genomics and genetics into the frog

(2012) Mustafa K. Khokha   GENESIS

Slc45a2 and V-ATPase are regulators of melanosomal pH homeostasis in zebrafish, providing a mechanism for human pigment evolution and disease

(2012) Christopher M. Dooley et al.   Pigment Cell & Melanoma Research

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis

(2012) S. Ishibashi et al.   Biology Open

Xenopus research: metamorphosed by genetics and genomics

(2011) Richard M. Harland et al.   TRENDS IN GENETICS

The Genome of the Western Clawed Frog Xenopus tropicalis

(2010) U. Hellsten et al.   SCIENCE

The Sequence Alignment/Map format and SAMtools

(2009) H. Li et al.   BIOINFORMATICS

Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly

(2009) H. J. Kim et al.   GENOME RESEARCH

MMEJ repair of double-strand breaks (director’s cut): deleted sequences and alternative endings

(2008) Mitch McVey et al.   TRENDS IN GENETICS

Leukocyte Tyrosine Kinase Functions in Pigment Cell Development

(2008) Susana S. Lopes et al.   PLoS Genetics