4.7 Article

Identification and determination of selenohomolanthionine - The major selenium compound in Torula yeast

Journal

FOOD CHEMISTRY
Volume 237, Issue -, Pages 1196-1201

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.06.042

Keywords

HPLC-ICP MS; Selenohomolanthionine; Yeast; Food supplement; HPLC-ESI Orbitrap MS

Funding

  1. Grants-in-Aid for Scientific Research [17H04001, 16H05812] Funding Source: KAKEN

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Torula yeast (Candida utilis) was found to metabolize selenium in a totally different way to Brewer's yeast (S. cerevisiae) leading to the biosynthesis of selenohomolanthionine (SeHLan), a major selenium compound accounting for 60-80% of the total selenium. The identity of SeHLan was confirmed by retention time matching in hydrophilic ion interaction chromatography (HILIC) with inductively coupled plasma mass spectrometric detection (ICP MS) using a custom synthesized standard molecule and by HILIC - Orbitrap MS and MS-MS fragmentation. Selenohomolanthionine escapes the current assays for the organic character of Se-rich yeast based on the protein-bound selenomethionine determination. A HILIC - ICP MS method was developed for the quantitative determination of selenohomolanthionine in yeast supplements with a detection limit of 146 ng/g. (C) 2017 Elsevier Ltd. All rights reserved.

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