4.7 Article

A modified FOX-1 method for Micro-determination of hydrogen peroxide in honey samples

Journal

FOOD CHEMISTRY
Volume 237, Issue -, Pages 225-231

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.05.065

Keywords

Honey; Hydrogen peroxide; FOX-1; Micro-determination

Funding

  1. National Natural Science Foundation of China [31272510]
  2. Science Foundation of Xi'an [NC1405(1)]
  3. Foundation of Education Department of Shaanxi Province Goverment [16JF025]
  4. China Agriculture Research System [CARS-45-KXJ10]

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Hydrogen peroxide (H2O2) is a major antibacterial activity-associated biomarker in honey. Measurement of endogenous H2O2 in honey is of great value in prediction of the H2O2-depended antibacterial activity and characterization or selection of honey samples for their use as an antibacterial agent or natural food preservative. Considering current methods for H2O2 determination are either time-consuming or complicated with their high-cost, a study was conducted to modify and validate the spectrophotometry-based ferrous oxidation-xylenol orange (FOX-1) method for micro-determination of H2O2 in honey samples. The result suggested that the proposed FOX-1 method is fast, sensitive, precise and repeatable. The method was successfully applied for the analysis of a total of 35 honey samples from 5 floral origins and 33 geographical origins. The proposed method is low-cost and easy-to-run, and it can be considered by researchers and industry for routine analysis. (C) 2017 Elsevier Ltd. All rights reserved.

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