Biochemical and structural characterization of CYP109A2, a vitamin D3 25-hydroxylase from Bacillus megaterium

Vitamin D supplementation guidelines

(2018) Pawel Pludowski et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

Investigating the effect of available redox protein ratios for the conversion of a steroid by a myxobacterial CYP260A1

(2017) Yogan Khatri et al.   FEBS LETTERS

Characterization of cytochrome P450 CYP109E1 from Bacillus megaterium as a novel vitamin D3 hydroxylase

(2017) Ammar Abdulmughni et al.   JOURNAL OF BIOTECHNOLOGY

Biotransformation of prednisone and dexamethasone by cytochrome P450 based systems – Identification of new potential drug candidates

(2017) Natalia Putkaradze et al.   JOURNAL OF BIOTECHNOLOGY

Structural insights into the mechanism of the drastic changes in enzymatic activity of the cytochrome P450 vitamin D3hydroxylase (CYP107BR1) caused by a mutation distant from the active site

(2017) Yoshiaki Yasutake et al.   Acta Crystallographica Section F-Structural Biology Communications

Applications of microbial cytochrome P450 enzymes in biotechnology and synthetic biology

(2016) Hazel M Girvan et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Structural basis of steroid binding and oxidation by the cytochrome P450 CYP109E1 fromBacillus megaterium

(2016) Ilona K. Jóźwik et al.   FEBS Journal

Expression of human CYP27A1 in B. megaterium for the efficient hydroxylation of cholesterol, vitamin D3 and 7-dehydrocholesterol

(2016) Maximilian Ehrhardt et al.   JOURNAL OF BIOTECHNOLOGY

A Novel NADPH-dependent flavoprotein reductase from Bacillus megaterium acts as an efficient cytochrome P450 reductase

(2016) Mohammed Milhim et al.   JOURNAL OF BIOTECHNOLOGY

Comparison of CYP106A1 and CYP106A2 from Bacillus megaterium – identification of a novel 11-oxidase activity

(2015) Flora Marta Kiss et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cytochromes P450 as promising catalysts for biotechnological application: chances and limitations

(2014) Rita Bernhardt et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

New Reactions and Products Resulting from Alternative Interactions between the P450 Enzyme and Redox Partners

(2014) Wei Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Deciphering key features in protein structures with the new ENDscript server

(2014) Xavier Robert et al.   NUCLEIC ACIDS RESEARCH

A new cytochrome P450 system from Bacillus megaterium DSM319 for the hydroxylation of 11-keto-β-boswellic acid (KBA)

(2013) Elisa Brill et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A Single Mutation at the Ferredoxin Binding Site of P450 Vdh Enables Efficient Biocatalytic Production of 25-Hydroxyvitamin D3

(2013) Yoshiaki Yasutake et al.   CHEMBIOCHEM

Cytochrome P450-mediated metabolism of vitamin D

(2013) Glenville Jones et al.   JOURNAL OF LIPID RESEARCH

Vitamin D for Health: A Global Perspective

(2013) Arash Hossein-nezhad et al.   MAYO CLINIC PROCEEDINGS

Towards automated crystallographic structure refinement withphenix.refine

(2012) Pavel V. Afonine et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Overview of theCCP4 suite and current developments

(2011) Martyn D. Winn et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

A new Bacillus megaterium whole-cell catalyst for the hydroxylation of the pentacyclic triterpene 11-keto-β-boswellic acid (KBA) based on a recombinant cytochrome P450 system

(2011) Sabrina Bleif et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genome Sequences of the Biotechnologically Important Bacillus megaterium Strains QM B1551 and DSM319

(2011) M. Eppinger et al.   JOURNAL OF BACTERIOLOGY

Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega

(2011) F. Sievers et al.   Molecular Systems Biology

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Bioconversion of vitamin D to its active form by bacterial or mammalian cytochrome P450

(2010) Toshiyuki Sakaki et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Three-step hydroxylation of vitamin D3 by a genetically engineered CYP105A1

(2010) Keiko Hayashi et al.   FEBS Journal

Structural Evidence for Enhancement of Sequential Vitamin D3Hydroxylation Activities by Directed Evolution of Cytochrome P450 Vitamin D3Hydroxylase

(2010) Yoshiaki Yasutake et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Purification, characterization, and directed evolution study of a vitamin D 3 hydroxylase from Pseudonocardia autotrophica

(2009) Yoshikazu Fujii et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Structure-Based Design of a Highly Active Vitamin D Hydroxylase fromStreptomyces griseolusCYP105A1†‡

(2008) Keiko Hayashi et al.   BIOCHEMISTRY

Daily oral 25-hydroxycholecalciferol supplementation for vitamin D deficiency in haemodialysis patients: effects on mineral metabolism and bone markers

(2008) G. Jean et al.   NEPHROLOGY DIALYSIS TRANSPLANTATION

Oxygen activation by cytochrome P450 monooxygenase

(2008) Djemel Hamdane et al.   PHOTOSYNTHESIS RESEARCH