Article
Immunology
Stephanie N. Blandford, Neva J. Fudge, Chris P. Corkum, Craig S. Moore
Summary: Extracellular vesicles (EVs) secreted from cells have emerged as potential biomarkers for neurological conditions, including multiple sclerosis (MS). This study found that immune cell-derived EVs were significantly increased in the plasma of untreated RRMS patients, despite limited changes in circulating immune cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Mengfei Xue, Siyuan Ye, Xiaopeng Ma, Fangfu Ye, Chen Wang, Ling Zhu, Yanlian Yang, Jianing Chen
Summary: This study demonstrates the importance of protein heterogeneity in tumor-derived small extracellular vesicles (sEVs) in cancer diagnosis. Using near-field infrared (nano-FTIR) spectroscopy, researchers were able to analyze the characteristics of individual sEVs and evaluate tumor malignancy and metastasis. The findings suggest that sEV protein heterogeneity can serve as a noninvasive biomarker for cancer diagnosis and contribute to our understanding of cancer development.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Immunology
Nicolas Sailliet, Matti Ullah, Amandine Dupuy, Amanda K. A. Silva, Florence Gazeau, Hoa Le Mai, Sophie Brouard
Summary: Extracellular vesicles (EVs) have been extensively studied and are known to actively regulate immune system functions through mechanisms such as protein-protein interactions and miRNA transfer. As potential targets for immunotherapies and potential biomarkers of graft rejection, EVs derived from immune cells show functional diversity.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Oncology
Andres Rincon-Riveros, Liliana Lopez, E. Victoria Villegas, Josefa Antonia Rodriguez
Summary: Extracellular vesicles, such as exosomes, have the potential to serve as biomarkers for cancer development, impacting various aspects of tumor progression and immune regulation.
Review
Biochemistry & Molecular Biology
Zhihang Zhou, Zihan Yang, Li Zhou, Mengsu Yang, Song He
Summary: TSPANs are a family of four-transmembrane proteins that are expressed on the cell surface, organelles and vesicles. They form tetraspanin-enriched microdomains with other membrane proteins to influence various cellular processes. TSPANs have been shown to regulate cancer cell growth, metastasis, drug resistance, as well as extracellular vesicle biogenesis and immunomicroenvironment.
CELL AND BIOSCIENCE
(2023)
Article
Medicine, Research & Experimental
Karla Rubio, Addi J. Romero-Olmedo, Pouya Sarvari, Guruprasadh Swaminathan, Vikas P. Ranvir, Diana G. Rogel-Ayala, Julio Cordero, Stefan Guenther, Aditi Mehta, Birgit Bassaly, Peter Braubach, Malgorzata Wygrecka, Stefan Gattenloehner, Achim Tresch, Thomas Braun, Gergana Dobreva, Miguel N. Rivera, Indrabahadur Singh, Johannes Graumann, Guillermo Barreto
Summary: A study revealed that non-canonical ITGB2 signaling activates EGFR and RAS/MAPK/ERK signaling in small cell lung cancer (SCLC), providing a potential target for therapy independent of EGFR mutations.
Review
Endocrinology & Metabolism
Rebecca S. Aguirre, Abhishek Kulkarni, Matthew W. Becker, Xiaoyong Lei, Soumyadeep Sarkar, Sasanka Ramanadham, Edward A. Phelps, Ernesto S. Nakayasu, Emily K. Sims, Raghavendra G. Mirmira
Summary: Islet β cells play a crucial role in the pathogenesis of type 1 diabetes, with extracellular vesicles (EVs) potentially serving as an important mechanism for cellular communication. Understanding the formation and cargo of EVs may offer new insights into T1D pathogenesis and potentially lead to novel therapeutic approaches. Future research in EV biology could provide opportunities for intervention in T1D.
MOLECULAR METABOLISM
(2022)
Review
Endocrinology & Metabolism
Rebecca S. Aguirre, Abhishek Kulkarni, Matthew W. Becker, Xiaoyong Lei, Soumyadeep Sarkar, Sasanka Ramanadham, Edward A. Phelps, Ernesto S. Nakayasu, Emily K. Sims, Raghavendra G. Mirmira
Summary: This review examines the impact of extracellular vesicles (EVs) released by pancreatic islet beta cells on the development of type 1 diabetes (T1D). The formation and cargo of EVs play important roles in the pathogenesis of T1D and have the potential to serve as biomarkers or therapeutic candidates.
MOLECULAR METABOLISM
(2022)
Article
Engineering, Biomedical
Runci Wang, Meng Hao, Xiaoxing Kou, Bingdong Sui, Maria Laura Sanmillan, Xiao Zhang, Dawei Liu, Jun Tian, Wenjing Yu, Chider Chen, Ruili Yang, Lingyun Sun, Yi Liu, Claudio Giraudo, Deepak A. Rao, Nan Shen, Songtao Shi
Summary: Mesenchymal stem cells influence T cells through extracellular vesicles, and in this study, we demonstrate the immunomodulatory potential of apoptotic vesicles generated by MSCs. Replenishing apoVs in Fas mutant mice rescued the deficiency and improved inflammation and lupus activity. ApoVs directly interacted with CD4+ T cells, inhibiting CD25 expression and IL-2 production.
BIOACTIVE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Jelle Penders, Anika Nagelkerke, Eoghan M. Cunnane, Simon Pedersen, Isaac J. Pence, R. Charles Coombes, Molly M. Stevens
Summary: The study focuses on extracellular vesicles (EVs) and introduces a high-throughput label-free EV analysis method for cancer diagnosis and monitoring, eliminating interpreter bias. By developing a dedicated device and using dimensional reduction analysis (DRA), a convenient and comprehensive approach for comparing multiple EV spectra is demonstrated. The study shows the high sensitivity and specificity of the dedicated SPARTA system in differentiating cancer and noncancer EVs, and its consistent predictive ability across multiple EV isolations.
Article
Engineering, Biomedical
Lilibeth Ortega-Pineda, Alec Sunyecz, Ana I. Salazar-Puerta, Maria Angelica Rincon-Benavides, Diego Alzate-Correa, Amrita Lakshmi Anaparthi, Elizabeth Guilfoyle, Louisa Mezache, Heather L. Struckman, Silvia Duarte-Sanmiguel, Binbin Deng, David W. McComb, Daniel J. Dodd, William R. Lawrence, Jordan Moore, Jingjing Zhang, Eduardo Reategui, Rengasayee Veeraraghavan, M. Tyler Nelson, Daniel Gallego-Perez, Natalia Higuita-Castro
Summary: Gene/oligonucleotide therapies have shown potential as a treatment strategy for neurological conditions, but current delivery methods have limitations. Extracellular vesicles (EVs) have emerged as a promising delivery vehicle, overcoming many of these limitations, including low permeability of the blood-brain barrier. This study provides a detailed understanding of the manufacturing process of neurogenic EVs and demonstrates their ability to enhance neuron-specific tropism and modulate electrophysiological activity. The study also suggests that surface decoration with glutamate receptors can facilitate enhanced delivery of EVs to the brain. Overall, these findings suggest that designer EVs loaded with specific factors can be a promising nanotechnology platform to drive pro-neuronal responses.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Immunology
Adrian E. Morelli, Yoel Sadovsky
Summary: This article explores the mechanisms underlying maternal tolerance of the semi- or fully-allogeneic fetus, with a focus on the role of extracellular vesicles (EVs) in immune modulation, providing a new perspective on immune suppression and activation during pregnancy.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Maria Eleonora Temperini, Flavio Di Giacinto, Sabrina Romano, Riccardo Di Santo, Alberto Augello, Raffaella Polito, Leonetta Baldassarre, Valeria Giliberti, Massimiliano Papi, Umberto Basile, Benedetta Niccolini, Ewa K. Krasnowska, Annalucia Serafino, Marco De Spirito, Alessandra Di Gaspare, Michele Ortolani, Gabriele Ciasca
Summary: This study demonstrates the use of mid-infrared plasmonic nanoantenna arrays to detect the specific vibrational absorption signal of EVs and the unspecific refractive index sensing signal. By immobilizing EVs on the gold nanoantenna surface through immunocapture, the researchers were able to selectively detect specific EV sub-populations and eliminate contaminants. The findings suggest that this lab-on-chip system has the potential to impact the development of novel laboratory medicine methods for the molecular characterization of EVs, due to its high protein sensitivity and ability to work with small sample volumes.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Cell Biology
Aida S. S. Hansen, Lea S. S. Jensen, Kristine R. R. Gammelgaard, Kristoffer G. G. Ryttersgaard, Christian Krapp, Jesper Just, Kasper L. L. Jonsson, Pia B. B. Jensen, Thomas Boesen, Mogens Johansen, Anders Etzerodt, Bent W. W. Deleuran, Martin R. R. Jakobsen
Summary: This article introduces the highly immunosuppressive tumor microenvironment, and explores a novel method to improve immunotherapy effectiveness by activating the STING signaling pathway. The researchers found that extracellular vesicles derived from activated CD4(+) T cells can sensitize macrophages and activate the STING pathway, effectively disrupting the immune suppressive environment in the tumor and priming for a robust immune response towards STING activation.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Immunology
Robert Ossendorff, Sibylle Grad, Tobias Tertel, Dieter C. Wirtz, Bernd Giebel, Verena Boerger, Frank A. Schildberg
Summary: Characterized mesenchymal stem/stromal cell-derived extracellular vesicles (MSC-EVs) have a positive effect on chondrocyte matrix production and show potential for treating osteoarthritis (OA). However, the functional heterogeneity of MSC-EV preparations needs to be addressed.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Felix Marsh-Wakefield, Pierre Juillard, Thomas M. Ashhurst, Annette Juillard, Diana Shinko, Givanna H. Putri, Mark N. Read, Helen M. McGuire, Scott N. Byrne, Simon Hawke, Georges E. Grau
Summary: B cells play a major role in multiple sclerosis (MS), and alemtuzumab therapy can restore B cell damage, which is of great significance in preventing the progression of the disease.
IMMUNOLOGY AND CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jian Tan, Duan Ni, Jemma Taitz, Gabriela Veronica Pinget, Mark Read, Alistair Senior, Jibran Abdul Wali, Ralph Nanan, Nicholas Jonathan Cole King, Georges Emile Grau, Stephen J. Simpson, Laurence Macia, Erin Shanahan, Huiling Wu, Steven J. Chadban, Reem Elnour
Summary: Secretory IgA is crucial for maintaining the balance between the host immune system and microbiota. This study reveals that dietary protein drives the production of secretory IgA through the release of extracellular vesicles by gut microbiota, independent of T-cell pathways or changes in microbiota composition.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, General & Internal
Rachel K. Ford, Pierre Juillard, Simon Hawke, Georges E. Grau, Felix Marsh-Wakefield
Summary: Cladribine treatment reduces trans-endothelial migration of T cell subsets in multiple sclerosis patients, contributing to disease control and maintenance of the integrity of the blood-brain barrier.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Aviva Levina, Anthony R. M. Chetcuti, Peter A. Lay
Summary: Ruthenium complexes are important in the development of metal-based anticancer drugs. This study investigates the interactions of the anticancer drugs KP1019 and [Ru-III(nta)(2)](3-) with transferrin in biological media and their cellular uptake in human hepatoma cells. The results show that the mode of transferrin binding and cellular uptake of ruthenium depend on the nature of the ruthenium complex and the availability of iron binding sites on transferrin, as well as the presence of competing proteins.
Article
Biochemistry & Molecular Biology
Amani M. Batarseh, Fatemeh Vafaee, Elham Hosseini-Beheshti, Azadeh Safarchi, Alex Chen, Amy Cohen, Annette Juillard, Nicholas Henry Hunt, Michael Mariani, Todd Mitchell, Georges Emile Raymond Grau
Summary: It is found that the pathogenesis of cerebral malaria can be understood by studying the specific lipid composition in the plasma. There are significant changes in lipid components in the plasma of cerebral malaria patients compared to non-cerebral malaria patients. This study provides important evidence for studying the pathophysiology of cerebral malaria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Linda Y. Lin, Pierre Juillard, Simon Hawke, Felix Marsh-Wakefield, Georges E. Grau
Summary: Multiple sclerosis (MS) is an autoimmune disease that damages the central nervous system (CNS) by destroying the myelin and forming demyelinating plaques. The migration of leukocytes across the blood-brain barrier (BBB) is a key factor in the development of MS. Oral cladribine has been shown to impact lymphocytes, but its effects on other leukocytes such as monocytes and dendritic cells (DCs) are not well understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Tamkin Ahmadzada, Abhishek Vijayan, Fatemeh Vafaee, Ali Azimi, Glen Reid, Stephen Clarke, Steven Kao, Georges E. Grau, Elham Hosseini-Beheshti
Summary: In this study, we characterized different populations of extracellular vesicles (EVs) derived from pleural mesothelioma cell lines and identified unique cancer-specific proteomes and oncogenic cargo in each subtype. We also established a comprehensive proteomic database of cancer-associated proteins in EVs, which could serve as new targets for future biomarker studies in pleural mesothelioma.
Article
Chemistry, Multidisciplinary
Kateryna Kostenkova, Aviva Levina, Drew A. Walters, Heide A. Murakami, Peter A. Lay, Debbie C. Crans
Summary: Two new series of complexes with pyridine-containing Schiff bases were synthesized and their chemical properties and stability were studied. One of the complexes exhibited good anti-proliferative activity in cell culture media, showing high selectivity towards glioblastoma cells and potential for drug treatment.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Serene Kuramarohit, Andria M. Yaourtis, Annie Nguyen, Michelle L. Wood, Aviva Levina, Peter A. Lay
Summary: Using IncuCyte Zoom imaging proliferation assays, it was observed that treatment with sub-toxic doses of [GaQ(3)] caused morphological changes and inhibition of cell migration in triple-negative human breast cancer cells. The addition of a trace amount of Cu(II) dramatically increased the cytotoxicity of [GaQ(3)]. The findings suggest the potential use of metal complexes in cancer therapy and the importance of ligand binding of essential metal ions for their cytotoxicity.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Inorganic & Nuclear
Aviva Levina, Canan Uslan, Heide Murakami, Debbie C. Crans, Peter A. Lay
Summary: Limited stability of transition-metal complexes in biological media has hindered their medicinal applications but also created potential for novel cancer treatments. This study found that two V(V) complexes decomposed in cell culture medium, but still showed high antiproliferative activities in breast cancer cells, with different mechanisms of action. Complex 1 is a suitable candidate for further development as an anticancer drug delivered via intratumoral injections.
INORGANIC CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Jian Tan, Jemma Taitz, Ralph Nanan, Georges Grau, Laurence Macia
Summary: This review discusses the relationship between dysbiosis and various diseases, and summarizes the dysbiotic gut-derived products observed in different diseases. Dysbiotic metabolites produced by the gut microbiota may contribute to disease development, while the reduced production of beneficial metabolites may be related to the high prevalence of inflammatory diseases in western countries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Parasitology
Adriana A. Marin, Annette Juillard, Alejandro M. Katzin, Leonardo J. M. Carvalho, Georges E. R. Grau
Summary: This study aims to analyze the effects of POH on human brain endothelial cells. The results showed that POH can prevent the sequestration of infected red blood cells in brain microvessels and restore the structure and function of brain endothelial cells, which has potential value in the treatment of cerebral malaria.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2023)
Article
Chemistry, Multidisciplinary
Kartika Wardhani, Aviva Levina, Biyun Sun, Georges E. R. Grau, F. Richard Keene, J. Grant Collins, Peter A. Lay
Summary: Using a new photoluminescent polypyridylruthenium(II) stain, researchers have uncovered previously unknown interactions between extracellular vesicles (EVs) released from lipopolysaccharide-stimulated THP-1 monocytes and the blood-brain barrier (BBB). These findings shed light on the impact of bacteria-induced immune response on BBB and provide insights into human brain diseases.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Liam J. J. Stephens, Elena Dallerba, Jenisi T. A. Kelderman, Aviva Levina, Melissa V. V. Werrett, Peter A. A. Lay, Massimiliano Massi, Philip C. C. Andrews
Summary: This study synthesized and characterized twelve Re(i) tricarbonyl diimine complexes with thiotetrazolato ligands. The tetrazolato ligand can bind to the Re(i) center through either the S atom or the N atom, with most complexes being bound to the N atom. In solution, the complexes exist as an equilibrating mixture of linkage isomers. The complexes exhibit photoluminescent properties, with emission wavelengths dependent on the nature of the diimine ligand.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Multidisciplinary
Serene Kuramarohit, Andria M. Yaourtis, Annie Nguyen, Michelle L. Wood, Aviva Levina, Peter A. Lay
Summary: This study demonstrates the potential use of a metal complex, GaQ(3), in differentiation anti-cancer therapy. It also shows that the cytotoxicity of GaQ(3) is strongly linked to the binding of essential metal ions, such as Cu(II), in the medium. These findings suggest that appropriate delivery mechanisms of such complexes and their ligands could lead to a powerful new approach for cancer chemotherapy.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)