Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Review
Cell Biology
Su-Ran Li, Qi-Wen Man, Xin Gao, Hao Lin, Jing Wang, Fu-Chuan Su, Han-Qi Wang, Lin-Lin Bu, Bing Liu, Gang Chen
Summary: Extracellular vesicles (EVs) are lipid-bilayer membrane structures secreted by most cell types, acting as messengers through the transfer of lipids, proteins, and nucleic acids to influence cellular processes. Tissue-derived EVs (Ti-EVs) offer advantages such as tissue specificity and accurate reflection of the tissue microenvironment, playing important roles in intercellular communication and disease development insights. Current research on Ti-EVs is limited compared to body fluid or cell culture-derived EVs, highlighting the need for further exploration in basic and clinical applications.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Chemistry, Multidisciplinary
Jelle Penders, Anika Nagelkerke, Eoghan M. Cunnane, Simon Pedersen, Isaac J. Pence, R. Charles Coombes, Molly M. Stevens
Summary: The study focuses on extracellular vesicles (EVs) and introduces a high-throughput label-free EV analysis method for cancer diagnosis and monitoring, eliminating interpreter bias. By developing a dedicated device and using dimensional reduction analysis (DRA), a convenient and comprehensive approach for comparing multiple EV spectra is demonstrated. The study shows the high sensitivity and specificity of the dedicated SPARTA system in differentiating cancer and noncancer EVs, and its consistent predictive ability across multiple EV isolations.
Review
Biochemistry & Molecular Biology
Laura Benecke, Mali Coray, Sandra Umbricht, Dapi Chiang, Fabricio Figueiro, Laurent Muller
Summary: Glioblastomas, highly aggressive tumors with low survival rates, create an immunosuppressive microenvironment through the transportation of biomolecules by exosomes. Recent studies have shown the significant impact of tumor-derived exosomes on immune cells, highlighting their potential as diagnostic tools and therapeutic targets for aggressive tumor cells like glioblastomas. Exosomes serve as important instruments for molecular understanding of tumors given their structural and functional properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Sarah Beck, Bernhard Hochreiter, Johannes A. Schmid
Summary: Extracellular vesicles (EVs) play crucial roles in intercellular communication by transferring nucleotides and proteins, and are associated with diseases such as cancer, inflammation, and thrombosis. They have the potential to serve as biomarkers for diagnosis and as vehicles for therapeutics in the future.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Medicine, General & Internal
M. Samuel, S. Gabrielsson
Summary: Utilizing exosomes for cancer immunotherapy involves considering them as an adjuvant-like delivery system for antigens and the role of antigen-presenting cells in inducing potent immune responses. Studies have shown that allogeneic exosomes could greatly improve the feasibility of new therapeutic approaches.
JOURNAL OF INTERNAL MEDICINE
(2021)
Review
Engineering, Biomedical
Shaobo Ruan, Zachary Greenberg, Xiaoshu Pan, Pei Zhuang, Nina Erwin, Mei He
Summary: Cancer immunotherapy has shown benefits in recent years, but novel targeting approaches and modulators are needed for broader patient populations. Extracellular vesicles (EVs) offer targeted immunity modulation and improved efficacy, with unique properties like surface markers and immunomodulatory functions. Future potential lies in EVs as a cancer immunotherapeutic biomaterial through reprogramming strategies and addressing unique challenges.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Oncology
Ilaria Casari, Justin Andrew Howard, Eunice Eugenia Robless, Marco Falasca
Summary: Pancreatic cancer, as one of the most lethal types of cancer, is difficult to diagnose and treat in time. The appearance of metastasis is dreaded by every cancer patient.
Article
Oncology
Mona Alharbi, Andrew Lai, Shayna Sharma, Priyakshi Kalita-de Croft, Nihar Godbole, America Campos, Dominic Guanzon, Alexis Salas-Burgos, Flavio Carrion, Felipe A. Zuniga, Lewis Perrin, Yaowu He, Tanja Pejovic, Carmen Winters, Terry Morgan, John D. Hooper, Gregory E. Rice, Carlos Salomon
Summary: Ovarian cancer is one of the most lethal cancers worldwide affecting women, with non-specific early symptoms. sEVs produced under low oxygen tension can induce a metabolic switch in ovarian cancer cells, promoting resistance to carboplatin. The presence of glycolysis pathway proteins in sEVs is closely associated with ovarian cancer recurrence.
Article
Oncology
Akira Yokoi, Takahiro Ochiya
Summary: This review summarizes recent studies on EVs from the perspective of EV heterogeneity and highlights their role in various steps from oncogenic to metastatic processes. Recognizing EV subtypes is crucial for identifying pathways affected by EVs and targeting specific subtypes in therapeutic approaches or liquid biopsies.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Oncology
V. Vlaeminck-Guillem
Summary: Exosomes play a significant role in cancer research, including their involvement in pathophysiological mechanisms, as biomarkers, and as therapeutic tools. However, their use in routine clinical practice and management of prostate cancer still requires further confirmation and generalization.
SEMINARS IN CANCER BIOLOGY
(2022)
Review
Oncology
Vadim V. Tarasov, Andrey A. Svistunov, Vladimir N. Chubarev, Samira A. Dostdar, Alexander V. Sokolov, Anna Brzecka, Olga Sukocheva, Margarita E. Neganova, Sergey G. Klochkov, Siva G. Somasundaram, Cecil E. Kirkland, Gjumrakch Aliev
Summary: This review explores the application of extracellular vesicles (EVs) as natural nanoparticles for drug delivery in cancer therapy, discussing aspects such as biogenesis, isolation, targeting, and loading of EVs. This research sheds light on the potential of EVs for targeted chemotherapy, gene therapy, and vaccine development.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Jafar Rezaie, Tahereh Etemadi, Maryam Feghhi
Summary: This article presents the current understanding of how exosomes regulate innate immune responses and discusses their potential applications in cancer therapy and immunotherapy.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Review
Endocrinology & Metabolism
Megan Ludwig, Rhea Rajvansh, Justin M. Drake
Summary: Contents within extracellular vesicles (EVs) have emerged as promising biomarkers that can provide valuable information about disease state and are acquired through noninvasive liquid biopsies. In prostate cancer, EVs play a crucial role in facilitating effective communication between cancer cells and the microenvironment, impacting important cellular processes such as metastasis, immune regulation, and drug resistance.
Review
Oncology
Akbar Lulu Marzan, Sarah Elizabeth Stewart
Summary: Pancreatic cancer is a highly lethal cancer with a 5-year survival rate of less than 10%, largely due to factors such as inadequate diagnostics, rapid metastasis, and chemoresistance. Research on extracellular vesicles in pancreatic cancer progression and chemoresistance is crucial for identifying new treatment options. Accumulating evidence suggests that extracellular vesicles may serve as biomarkers for early detection and as potential therapeutic targets or drug delivery tools for the treatment of pancreatic cancer.
Review
Oncology
Nelson Rangel, Victoria E. Villegas, Milena Rondon-Lagos
Summary: Obesity is a growing health challenge and is recognized as a risk factor for breast cancer. While the exact mechanisms linking obesity to breast cancer are not fully understood, this association has been linked to impaired hormone secretion by dysfunctional adipose tissue. Altered secretion of androgens and adipokines are independently associated with breast cancer development, highlighting the potential for crosstalk between these factors and the androgen receptor signaling in cancer cells. Understanding these interactions is crucial for guiding the development of new therapeutic approaches aimed at preventing and treating obesity and breast cancer.
Review
Biology
Paola Cruz-Tapias, Wilson Rubiano, Milena Rondon-Lagos, Victoria-E Villegas, Nelson Rangel
Summary: Studies have shown that high levels of AR mRNA may serve as a promising non-invasive prognostic biomarker, aiding in the identification of less aggressive BC subtypes.
Review
Biochemistry & Molecular Biology
Andres Rincon-Riveros, Duvan Morales, Josefa Antonia Rodriguez, Victoria E. Villegas, Liliana Lopez-Kleine
Summary: Noncoding RNAs play key roles in gene expression regulation by interacting with other biological molecules, and computational biology can enhance our understanding of these interactions. Experimental findings drive advancements in computational methods, which in turn provide new insights for experimental validation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Andres Rincon-Riveros, Jairo De la Pena, Wilson Rubiano, Fabio Olivella, Maria Martinez-Aguero, Victoria E. Villegas
Summary: This article presents a case of primary breast angiosarcoma, providing insights into the clinical features, immunohistochemical properties, and genetic characteristics of the tumor. These studies contribute to the expanding knowledge of this rare disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Andres Rincon-Riveros, Josefa Antonia Rodriguez, Victoria E. Villegas, Liliana Lopez-Kleine
Summary: This study identified two commonly deregulated miRNAs in exosomes from healthy individuals and cancer patients, which had implications for functional processes through the examination of their target genes.
Article
Oncology
Natalia Vargas-Rondon, Erika Perez-Mora, Victoria E. Villegas, Milena Rondon-Lagos
CANCER BIOLOGY & MEDICINE
(2020)
Review
Oncology
Andrea Luque-Bolivar, Erika Perez-Mora, Victoria Eugenia Villegas, Milena Rondon-Lagos
BREAST CANCER-TARGETS AND THERAPY
(2020)