Article
Genetics & Heredity
Anna Schmuecker, Bingkun Lei, Zdravko J. Lorkovic, Matias Capella, Sigurd Braun, Pierre Bourguet, Olivier Mathieu, Karl Mechtler, Frederic Berger
Summary: The selection of C-terminal motifs contributes to the evolution of distinct histone H2A variants, with cell cycle dependent kinases playing a crucial role. Interference between the functions carried by these motifs led to their mutual exclusion and the evolution of different classes of H2A variants.
Review
Cell Biology
Aidan J. Levinsky, Gregor McEdwards, Nasha Sethna, Mark A. Currie
Summary: H3K9 methyltransferases play crucial roles in genome stability, cell type-specific gene expression, and non-histone methylation. They are involved in histone modification and regulate the methylation of various non-histone targets, contributing to genome regulation and cellular functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Yukiko Kuroda, Aiko Iwata-Otsubo, Kerith-Rae Dias, Suzanna E. L. Temple, Koji Nagao, Lachlan De Hayr, Ying Zhu, Shin-Ya Isobe, Gohei Nishibuchi, Sarah K. Fiordaliso, Yuki Fujita, Alyssa L. Rippert, Samuel W. Baker, Marco L. Leung, Daniel C. Koboldt, Adele Harman, Beth A. Keena, Izumi Kazama, Gopinath Musuwadi Subramanian, Kandamurugu Manickam, Betsy Schmalz, Maeson Latsko, Elaine H. Zackai, Matt Edwards, Carey-Anne Evans, Matthew C. Dulik, Michael F. Buckley, Toshihide Yamashita, W. Timothy O'Brien, Robert J. Harvey, Chikashi Obuse, Tony Roscioli, Kosuke Izumi
Summary: This study identified heterozygous de novo variants in the CBX1 gene, encoding HP1β, as a cause of a novel syndromic neurodevelopmental disorder. In vitro cellular assays, neurobehavioral and cytological analyses of neuronal cells, and mouse models confirmed the pathogenicity of the identified variants. The disruption of HP1β chromatin binding during neurocognitive development contributes to developmental disabilities.
GENETICS IN MEDICINE
(2023)
Article
Multidisciplinary Sciences
Michael Habig, Cecile Lorrain, Alice Feurtey, Jovan Komluski, Eva H. Stukenbrock
Summary: In this study, the authors demonstrated a causal effect of epigenetic modifications on mutation rates in fungal pathogens, as well as the impact of temperature stress and cytosine methylation on the rate and location of spontaneous mutations in the genome. Additionally, they found that histone modifications and transposable elements can significantly affect mutation rates.
NATURE COMMUNICATIONS
(2021)
Review
Plant Sciences
Rocio Nunez-Vazquez, Benedicte Desvoyes, Crisanto Gutierrez
Summary: Plants have developed various mechanisms to adapt to abiotic stresses, which involve transcriptional regulation and chromatin modifications. Understanding and manipulating these regulatory networks are crucial for enhancing crop resilience and performance against stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Genetics & Heredity
Alejandra Agredo, Andrea L. Kasinski
Summary: Chromatin is regulated by histone proteins through various post-translational modifications, especially tri-methylation of histone H4 on lysine 20 (H4K20me3), which plays important roles in chromatin structure, cell cycle, DNA repair, and development. Recent studies have provided insights into the establishment and regulation of H4K20me3 and its involvement in various diseases through interactions with other proteins. This review aims to summarize the current knowledge of H4K20me3 function and regulation, as well as highlight remaining gaps in the field.
FRONTIERS IN GENETICS
(2023)
Article
Cell Biology
Pe'era Wasserzug Pash, Gilad Karavani, Eli Reich, Lital Zecharyahu, Zehava Kay, Dvora Bauman, Talya Mordechai-Daniel, Tal Imbar, Michael Klutstein
Summary: Pre-pubertal oocytes are dormant and do not undergo meiotic divisions naturally. Pubertal transition triggers molecular changes and epigenetic events in oocytes. Our study shows that pubertal transition leads to reorganization of oocyte chromatin and elevation of histone methylation levels. Understanding these changes is important for developing methods to mature pre-pubertal oocytes for fertility preservation in young cancer survivors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Food Science & Technology
Aurelie Etier, Fabien Dumetz, Sylvain Chereau, Nadia Ponts
Summary: Studies have shown that histone post-translational modifications play a crucial role in biological processes of fungi, particularly in regulating primary and secondary metabolisms of filamentous fungi.
Article
Oncology
Carla Guenther, Imrul Faisal, Manlio Fusciello, Maria Sokolova, Heidi Harjunpaa, Mette Ilander, Robert Tallberg, Maria Kristina Vartiainen, Ronen Alon, Jose-Maria Gonzalez-Granado, Vincenzo Cerullo, Susanna Carola Fagerholm
Summary: The study found that bone marrow-derived DCs expressing dysfunctional beta 2-integrin adhesion receptors displayed enhanced tumor rejection capabilities in melanoma models, showing spontaneous maturation. This maturation was associated with alterations in the cellular epigenetic/transcriptional profile and a potential role of integrins in regulating cell function.
CANCER IMMUNOLOGY RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Qiong Wu, Anders E. Berglund, Arnold B. Etame
Summary: Glioblastoma is highly resistant to standard therapies and the durability of response to the best chemotherapy agent, Temozolomide, is often short-lived due to tumor resistance. The need for therapies that can provide synergy to chemoradiation is urgent, as adaptive resistance evolution in GBM is facilitated through treatment-induced epigenetic modifications. Understanding and targeting these epigenetic modifications associated with GBM resistance is a top priority.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Jun-Li Wang, Dong-Wei Di, Pan Luo, Li Zhang, Xiao-Feng Li, Guang-Qin Guo, Lei Wu
Summary: This review focuses on the molecular mechanisms through which epigenetic modifications regulate auxin biosynthesis, demonstrating that complex signaling pathways affect gene expression and subsequently protein production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Julian Broche, Goran Kungulovski, Pavel Bashtrykov, Philipp Rathert, Albert Jeltsch
Summary: This study revealed that DNA methylation introduced in CGIs can significantly decrease gene expression, and is associated with a global decrease in H3K4me3 and H3K27ac, while being stable at some CGIs. The results highlight the importance of genome-wide molecular processes that protect CGIs against DNA methylation.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Oncology
Francisco Gimeno-Valiente, Gerardo Lopez-Rodas, Josefa Castillo, Luis Franco
Summary: This review focuses on the interconnections between epigenetics and alternative splicing in the development of cancer. It discusses the mechanisms involved in these interconnections and the potential diagnostic and therapeutic tools that can be derived from them. The reversible nature of epigenetic alterations and the possibility of correcting aberrant alternative splicing offer promising therapeutic possibilities for cancer treatment.
Review
Cell Biology
Faith M. Joseph, Nicolas L. Young
Summary: Post-translational modifications (PTMs) of histones play a key role in DNA-based processes and contribute to cell differentiation and gene function by adding an extra layer of regulation. Variations in histone sequences within each family of histones expand the chromatin repertoire and provide further mechanisms for regulation and signaling. However, much remains unknown about variant-specific PTMs and their role in regulating chromatin due to limited technologies and appropriate reagents.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Plant Sciences
Andrew R. Leitch, Lu Ma, Steven Dodsworth, Joerg Fuchs, Andreas Houben, Ilia J. Leitch
Summary: Angiosperm genomes consist of genes and their regulatory regions, repeats, semi-degraded repeats, and "dark matter". This study compares the histone modifications associated with chromatin packaging of these different genomic components in two species with significantly different genome sizes. The results reveal distinct associations between certain histone marks and different genomic features, providing insights into epigenetic profiles and chromatin organization.
Article
Oncology
Boris Redko, Helena Tuchinsky, Tamar Segal, Dror Tobi, Galia Luboshits, Osnat Ashur-Fabian, Albert Pinhasov, Gabi Gerlitz, Gary Gellerman
Article
Microbiology
Kira Kondratyeva, Ayala Wollman, Gabi Gerlitz, Shiri Navon-Venezia
JOURNAL OF MEDICAL MICROBIOLOGY
(2017)
Article
Cell Biology
Tamar Segal, Mali Salmon-Divon, Gabi Gerlitz
Article
Neurosciences
Liraz Keidar, Gabi Gerlitz, Aditya Kshirsagar, Michael Tsoory, Tsviya Olender, Xing Wang, Ying Yang, Yu-Sheng Chen, Yun-Gui Yang, Irina Voineagu, Orly Reiner
FRONTIERS IN CELLULAR NEUROSCIENCE
(2019)
Review
Cell Biology
Gabi Gerlitz
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell Biology
Andrea Fracchia, Tal Asraf, Mali Salmon-Divon, Gabi Gerlitz
Article
Oncology
Francesco Roncato, Ofer Regev, Sara W. Feigelson, Sandeep Kumar Yadav, Lukasz Kaczmarczyk, Nehora Levi, Diana Drago-Garcia, Samuel Ovadia, Marina Kizner, Yoseph Addadi, Joao C. Sabino, Yossi Ovadya, Sergio F. de Almeida, Ester Feldmesser, Gabi Gerlitz, Ronen Alon
Summary: Research indicates that reduced levels of A-type lamins in highly metastatic cancer cells do not increase their ability to pass through endothelial barriers and lung vessels, but may instead compromise lung metastasis at a later stage.
Article
Chemistry, Multidisciplinary
Lukasz S. Kaczmarczyk, Katherine S. Marsay, Sergey Shevchenko, Moritz Pilossof, Nehora Levi, Moshe Einat, Matan Oren, Gabi Gerlitz
Summary: 95 GHz waves have been shown to efficiently kill coronavirus and poliovirus, significantly reducing the virus titer and forming holes in the virus envelope. These waves can reduce the coronavirus titer to a low infective dose range for humans and animals, making them an effective method for decontaminating objects.
ENVIRONMENTAL CHEMISTRY LETTERS
(2021)
Article
Oncology
Sarah Hofmann, Raichel Cohen-Harazi, Yael Maizels, Igor Koman
Summary: The study successfully grew in vitro spheroids from breast cancer tissue, showing significant implications for exploring cancer therapeutics and personalized treatment efficacy, providing a new tool for clinical personalized medicine applications.
TRANSLATIONAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Lukasz Stanislaw Kaczmarczyk, Nehora Levi, Tamar Segal, Mali Salmon-Divon, Gabi Gerlitz
Summary: More than one third of the mammalian genome is associated with the nuclear lamina, called lamina-associated domains (LADs), which play a fundamental role in chromatin biology. LADs association with the nuclear envelope relies on the interaction between nuclear membrane proteins and heterochromatin modifications, as well as proteins that bind the borders of LADs. CTCF is identified as a key factor in assisting the association of facultative, short LADs with the nuclear lamina upon chromatin stress.
CHROMOSOME RESEARCH
(2022)
Article
Cell Biology
Rosari Hernandez-Vicens, Jagreeti Singh, Nomi Pernicone, Tamar Listovsky, Gabi Gerlitz
Summary: Our study suggests that SETDB1 affects microtubule dynamics by interacting with both microtubules and HDAC6 to enhance tubulin deacetylation. These findings provide a novel insight into the cytoplasmic role of SETDB1 in the regulation of microtubule dynamics.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Santosh Kumar, Valid Gahramanov, Shivani Patel, Julia Yaglom, Lukasz Kaczmarczyk, Ivan A. Alexandrov, Gabi Gerlitz, Mali Salmon-Divon, Michael Y. Sherman
Summary: Resistance to chemotherapy is commonly caused by mutations or changes in protein expression. The selection of drug-resistant mutants can occur randomly prior to treatment or through the generation of mutations during treatment. Here, we studied the origin of resistance mutations to the Top1 inhibitor irinotecan and found that the mutations accumulated in non-coding regions of DNA at Top1-cleavage sites. Cancer cells had a higher number of these sites compared to the reference genome, contributing to their increased sensitivity to irinotecan. These mutations gradually reduced the generation of DNA breaks and increased drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Katherine Chiu, Yasmin Berrada, Nebiyat Eskndir, Dasol Song, Claire Fong, Sarah Naughton, Tina Chen, Savanna Moy, Sarah Gyurmey, Liam James, Chimere Ezeiruaku, Caroline Capistran, Daniel Lowey, Vedang Diwanji, Samantha Peterson, Harshini Parakh, Ayanna R. Burgess, Cassandra Probert, Annie Zhu, Bryn Anderson, Nehora Levi, Gabi Gerlitz, Mary C. Packard, Katherine A. Dorfman, Michael Seifu Bahiru, Andrew D. Stephens
Summary: Mitosis is a crucial process that ensures equal segregation of duplicated genome into two daughter cells. Recent studies have shown that CTCF plays an important role in mitosis by localizing CENP-E. In this study, using CRISPR-based genetic knockdowns, the researchers examined the impact of CTCF on mitotic fidelity. They found that CTCF knockdown resulted in prolonged mitoses and failed anaphase segregation. The knockdown also led to abnormal mitotic spindle organization and increased nuclear size. These findings highlight the significance of CTCF in both metaphase organization and anaphase segregation during mitosis.
