Article
Multidisciplinary Sciences
Daniel P. Singh, Luke Pearce, Rocky H. Choi, Aldo Meizoso-Huesca, Stefan G. Wette, John W. Scott, Cedric R. Lamboley, Robyn M. Murphy, Bradley S. Launikonis
Summary: Resting skeletal muscle generates heat for endothermy in mammals but not amphibians, through the regulation of Ca2+ and the sympathetic nervous system. By studying muscle fibers in amphibians and mammals, it was found that mammals have a resistance to Ca2+-activated regenerative Ca2+ release and heat generation is regulated by cAMP-activated RyR Ca2+ leak. This indicates that the loss of a Ca2+-sensitive RyR isoform was an important evolutionary event for heat generation in mammals.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Edson Assuncao Mareco, Daniel Garcia de la Serrana, Tassiana Gutierrez de Paula, Bruna Tereza Thomazini Zanella, Bruno Oliveira da Silva Duran, Rondinelle Artur Simoes Salomao, Bruno Evaristo de Almeida Fantinatti, Victor Hugo Garcia de Oliveira, Vander Bruno dos Santos, Robson Francisco Carvalho, Maeli Dal-Pai-Silva
Summary: Using RNA-Seq analysis, we characterized the transcriptome of white muscle from Pacu, Tambaqui, and their interspecific hybrid (Tambacu). Comparative analysis revealed a higher number of expressed genes in the hybrid genotype. We also identified target genes related to cellular processes through differential expression analysis and validated their expression using RT-qPCR.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Neeraj Agarwal, Qiong Zhou, Deepak Arya, Sebastien Rinaldetti, Jason Duex, Daniel LaBarbera, Dan Theodorescu
Summary: This study reveals a signaling pathway that explains the upregulated expression of hTERT caused by the -124 hTERT promoter mutation in bladder cancer. Through high-content screening drug discovery using a GFP mutated cell line, a novel drug inhibitor AST-487 was identified, which can inhibit both wild-type and mutant-driven hTERT expression. The study also identifies the RET kinase pathway as a new regulator of mutant hTERT promoter-driven transcription.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Nathalie Olympios, Vianney Gilard, Florent Marguet, Florian Clatot, Frederic Di Fiore, Maxime Fontanilles
Summary: Glioblastoma is the most common malignant primary brain tumor in adults, with an incidence of 2 to 3 cases per 100,000 persons in North America and Europe. Alterations in the telomerase reverse transcriptase promoter, highly recurrent in all glioblastomas, including both IDH wild type and mutated, are potential mechanisms enabling cancer growth.
Article
Biochemistry & Molecular Biology
Francois Hafezi, Lisa Jaxel, Morgane Lemaire, Jonathan D. Turner, Danielle Perez-Bercoff
Summary: TERT promoter mutations, especially the -124C>T mutation, play a crucial role in the reactivation of telomerase reverse transcriptase. The mutations generally lead to increased TERT transcription levels, with the double mutation showing the highest impact. Additionally, the presence of these mutations may unleash antisense transcription and lncRNA TAPAS might help balance the effects of TERT promoter mutations.
Article
Agriculture, Dairy & Animal Science
Jessica Maria Abbate, Gabriele Grifo, Fabiano Capparucci, Francesca Arfuso, Serena Savoca, Luca Cicero, Giancarlo Consolo, Giovanni Lanteri
Summary: This study explores the use of an oscilloscope coupled with a signal generator to evaluate changes in the electrical conductivity of skeletal muscle and find a quantitative parameter for postmortem interval (PMI) estimation. The results show that the oscilloscope, especially the root mean square (RMS) measured parameter, is a promising technology for studying muscle properties during the early postmortem interval. It is a rapid, non-destructive, and inexpensive method. However, further studies are needed to validate this technology for real-time field investigations.
Review
Oncology
Sivaramakrishna Rachakonda, Jorg D. Hoheisel, Rajiv Kumar
Summary: Telomere shortening acts as a tumor suppressor by restricting replicative potential, while cancer cells evade this limitation through telomerase reactivation. Mutations in the TERT promoter increase TERT transcription, delaying senescence but not preventing telomere shortening. The scarcity of functional mutations outside coding sequences suggests evolutionary constraints on human genomes.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Review
Oncology
Liu Yang, Na Li, Meng Wang, Yan-Hua Zhang, Lu-Da Yan, Wen Zhou, Zhi-Qiong Yu, Xiao-Chun Peng, Jun Cai
Summary: Non-small cell lung cancer (NSCLC) accounts for a majority of lung cancer cases and is commonly diagnosed at an advanced stage with low survival rates. Activation of telomerase, particularly through telomerase reverse transcriptase (TERT), is associated with the malignant characteristics and prognosis of NSCLC, offering potential therapeutic applications for the treatment of this disease.
Article
Cell & Tissue Engineering
Diana L. Carlone, Rebecca D. Riba-Wolman, Luke T. Deary, Alessio Tovaglieri, Lijie Jiang, Dana M. Ambruzs, Benjamin E. Mead, Manasvi S. Shah, Christopher J. Lengner, Rudolf Jaenisch, David T. Breault
Summary: The study found that mTert-expressing cells are enriched during adolescent bone growth, with these cells having enriched colony-forming capacity and contributing to multiple mesenchymal lineages, making them a distinct population of SSCs. mTert(+) cells were identified as osteochondral progenitors, contributing to the bone-forming cell pool during endochondral bone growth, with some cells persisting into adulthood.
Article
Biochemistry & Molecular Biology
Petr Fajkus, Vratislav Peska, Jiri Fajkus, Eva Sykorova
Summary: This study examined the presence and functional status of TERT variants in allotetraploid Nicotiana species of diverse evolutionary ages, as well as in other diploid and polyploid plant species. Results showed various patterns of retention or loss of parental TERT variants and divergence in their functions, with all identified TERT variants confirmed to be expressed. Synteny analyses revealed the conservation and translocation of TERT genes in representative plant genomes, with translocation becoming fixed in target loci in various diploid and polyploid species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
D. P. Nagahawatta, N. M. Liyanage, Thilina U. Jayawardena, H. H. A. C. K. Jayawardhana, Jae-Young Oh, K. K. Asanka Sanjeewa, Sang In Kang, You-Jin Jeon
Summary: The study found that digested flounder meat has the potential to promote skeletal muscle growth and can be used as a functional food ingredient.
Article
Oncology
Jinglong Zhang, Fan Zhang, Kenneth I. Porter, Panshak P. Dakup, Shuwen Wang, Gavin P. Robertson, Shobhan Gaddameedhi, Jiyue Zhu
Summary: Telomerase activation is crucial for melanomagenesis, but is hindered by telomere dysfunction in mice. The study found that telomere dysfunction delays melanoma development and leads to DNA damage and genomic instability.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Genetics & Heredity
Bruna Petry, Gabriel Costa Monteiro Moreira, Aline Goncalves Lio Copola, Marcela Maria de Souza, Fernanda Cristina da Veiga, Erika Cristina Jorge, Jane de Oliveira Peixoto, Monica Correa Ledur, James E. Koltes, Luiz Lehmann Coutinho
Summary: This study investigated the role of SAP30 gene in muscle development by downregulating it in C2C12 muscle cell culture and analyzing its impact on morphometric traits and gene expression. The results showed that modulation of SAP30 expression increased myotube area, indicating its involvement in muscle hypertrophy. RNA-seq analysis further supported the role of SAP30 gene in regulating muscle development.
FRONTIERS IN GENETICS
(2021)
Review
Genetics & Heredity
Maha Sellami, Nicola Bragazzi, Mohammad Shoaib Prince, Joshua Denham, Mohamed Elrayess
Summary: Exercise training can delay the onset of age-related diseases, affect telomere length, and impact DNA epigenetic modifications, playing a significant role in biological aging.
FRONTIERS IN GENETICS
(2021)
Article
Multidisciplinary Sciences
Noriaki Minami, Donghyun Hong, Celine Taglang, Georgios Batsios, Anne Marie Gillespie, Pavithra Viswanath, Nicholas Stevers, Carter J. Barger, Joseph F. Costello, Sabrina M. Ronen
Summary: The expression of TERT and GABPB1 modulates flux via the pentose phosphate pathway. Silencing of TERT or GABPB1 leads to a reduction in PPP flux, as demonstrated by C-13 magnetic resonance spectroscopy. This study suggests the potential of targeting TERT or GABPB1 as a therapeutic strategy.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Biomedical
Jin Liu, Lei Dang, Defang Li, Chao Liang, Xiaojuan He, Heng Wu, Airong Qian, Zhijun Yang, Doris W. T. Au, Michael W. L. Chiang, Bao-Ting Zhang, Quanbin Han, Kevin K. M. Yue, Hongqi Zhang, Changwei Lv, Xiaohua Pan, Jiake Xu, Zhaoxiang Bian, Peng Shang, Weihong Tan, Zicai Liang, Baosheng Guo, Aiping Lu, Ge Zhang
Article
Biotechnology & Applied Microbiology
Keng Po Lai, Jing-Woei Li, Simon Yuan Wang, Jill Man-Ying Chiu, Anna Tse, Karen Lau, Si Lok, Doris Wai-Ting Au, William Ka-Fai Tse, Chris Kong-Chu Wong, Ting- Fung Chan, Richard Yuen-Chong Kong, Rudolf Shiu-Sun Wu
Article
Biochemistry & Molecular Biology
Frauke Seemann, Drew R. Peterson, P. Eckhard Witten, Bao-Sheng Guo, Adamane H. Shanthanagouda, Rui R. Ye, Ge Zhang, Doris W. T. Au
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2015)
Article
Engineering, Environmental
Lianguo Chen, Jin Sun, Huoming Zhang, Doris W. T. Au, Paul K. S. Lam, Weipeng Zhang, Vladimir B. Bajic, Jian-Wen Qiu, Pei-Yuan Qian
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2015)
Article
Genetics & Heredity
Bo-Mi Kim, Beom-Soon Choi, Hui-Soo Kim, Jae-Sung Rhee, Doris W. T. Au, Rudolf S. S. Wu, Ik-Young Choi, Jae-Seong Lee
Article
Biochemistry & Molecular Biology
Chao Liang, Baosheng Guo, Heng Wu, Ningsheng Shao, Defang Li, Jin Liu, Lei Dang, Cheng Wang, Hui Li, Shaohua Li, Wing Ki Lau, Yu Cao, Zhijun Yang, Cheng Lu, Xiaojuan He, D. W. T. Au, Xiaohua Pan, Bao-Ting Zhang, Changwei Lu, Hongqi Zhang, Kinman Yue, Airong Qian, Peng Shang, Jiake Xu, Lianbo Xiao, Zhaoxiang Bian, Weihong Tan, Zicai Liang, Fuchu He, Lingqiang Zhang, Aiping Lu, Ge Zhang
Review
Environmental Sciences
Bo-Mi Kim, Jaebum Kim, Ik-Young Choi, Sheikh Raisuddin, Doris W. T. Au, Kenneth M. Y. Leung, Rudolf S. S. Wu, Jae -Sung Rhee, Jae-Seong Lee
MARINE ENVIRONMENTAL RESEARCH
(2016)
Article
Multidisciplinary Sciences
Defang Li, Jin Liu, Baosheng Guo, Chao Liang, Lei Dang, Cheng Lu, Xiaojuan He, Hilda Yeuk-Siu Cheung, Liang Xu, Changwei Lu, Bing He, Biao Liu, Atik Badshah Shaikh, Fangfei Li, Luyao Wang, Zhijun Yang, Doris Wai-Ting Au, Songlin Peng, Zongkang Zhang, Bao-Ting Zhang, Xiaohua Pan, Airong Qian, Peng Shang, Lianbo Xiao, Baohong Jiang, Chris Kong-Chu Wong, Jiake Xu, Zhaoxiang Bian, Zicai Liang, De-an Guo, Hailong Zhu, Weihong Tan, Aiping Lu, Ge Zhang
NATURE COMMUNICATIONS
(2016)
Article
Engineering, Environmental
Lianguo Chen, Chenyan Hu, Mirabelle M. P. Tsui, Teng Wan, Drew R. Peterson, Qipeng Shi, Paul K. S. Lam, Doris W. T. Au, James C. W. Lam, Bingsheng Zhou
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2018)
Article
Marine & Freshwater Biology
Roy R. Ye, Drew R. Peterson, Shin-Ichi Kitamura, Helmut Segner, Frauke Seemann, Doris W. T. Au
AQUATIC TOXICOLOGY
(2018)
Article
Environmental Sciences
C. C. Fong, Y. F. Shi, W. K. Yu, F. Wei, J. P. van de Merwe, Alice K. Y. Chan, R. Ye, Doris W. T. Au, Rudolf S. S. Wu, M. S. Yang
MARINE POLLUTION BULLETIN
(2014)
Article
Multidisciplinary Sciences
Admane H. Shanthanagouda, Bao-Sheng Guo, Rui R. Ye, Liang Chao, Michael W. L. Chiang, Gopalakrishnan Singaram, Napo K. M. Cheung, Ge Zhang, Doris W. T. Au
Article
Biochemistry & Molecular Biology
Gabriela Maressa Machado Baesso, Amanda Vitoria Venancio, Livia Costa Varela Barca, Pepita Fernanda Peppi, Caique Aparecido Faria, Joao Pedro Cosmo Machado, Luiz David Solis Murgas, Alvaro Jose de Almeida Bicudo, Eduardo Mauricio Mendes de Lima, Andre Rodrigues da Cunha Barreto-Vianna
Summary: This study evaluated the effects of eugenol, menthol, and lidocaine as anesthetics on glucose levels and recovery times in zebrafish. The results showed that eugenol and menthol did not affect glucose levels, while lidocaine caused non-concentration-dependent hyperglycemia. Recovery times were similar for eugenol and menthol, but concentration-dependent for lidocaine. Eugenol and menthol were deemed safe and satisfactory for glucose analysis in zebrafish research, while lidocaine could introduce biases and safety concerns. Researchers should carefully consider the selection of anesthetics for reliable results in zebrafish assessments.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Xiaoxue Li, Tangsong Feng, Weiqun Lu
Summary: This study reveals that exposure to VPA induces ASD-like phenotypes and behaviors, including gene transcription changes, social interaction deficit, decreased aggression, increased anxiety behavior, and elevated cortisol levels. The aggression behavior assay is a better predictor of neurotoxicology behavior of VPA.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Garam An, Junho Park, Whasun Lim, Gwonhwa Song
Summary: In this study, we investigated the hepatotoxicity and mechanism of action of pyridaben using zebrafish and ZFL cells. Pyridaben caused liver size reduction and apoptosis, and these effects were associated with uncontrolled intracellular calcium flow and downregulation of the PI3K/Akt signaling cascade.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Md Alamgir Kabir, Md. Golam Rabbane, Marco R. Hernandez, Md Aftab Ali Shaikh, Mohammad Moniruzzaman, Xuexiu Chang
Summary: This study investigated the toxicity of high concentrations of cadmium (Cd) on intestinal immunity and microbial diversity in common carp. The results showed that high Cd concentration caused intestinal inflammation, immunosuppression, and changes in the gut microbiome, leading to detrimental effects on the intestinal homeostasis and health status of the fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Anuradha Batabyal, Veronica Rivi, Cristina Benatti, Johanna M. C. Blom, Fabio Tascedda, Ken Lukowiak
Summary: This study found that exposure to heat shock stressors and bacterial lipopolysaccharide can induce the Garcia effect in pond snails. Additionally, aspirin can prevent the formation of the Garcia effect. However, unexpectedly, exposure to crushed aspirin and heat shock stressors together leads to long-lasting feeding suppression and molecular changes in the snails' serotoninergic system and neuroplasticity genes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Li-Yih Lin, Chieh-An Cheng, Sian-Tai Liu, Jiun-Lin Horng
Summary: This study reveals that ammonia exposure impairs ion regulation in zebrafish embryos by inducing oxidative stress, mitochondrial dysfunction, and cell death. The findings suggest that ammonia is toxic to aquatic animals and can lead to the death of embryos.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Antonio Durate Pagano, Natieli Machado Goncalves, William Borges Domingues, Tony Leandro Rezende da Silveira, Mateus Tavares Kutter, Antonio Sergio Varela Jr, Carine Dahl Corcini, Mariana Cavalcanti Nascimento, Luana Ferreira Viana dos Reis, Patricia Gomes Costa, Adalto Bianchini, Matheus Vieira Volcan, Mariana Harter Remia, Vinicius Farias Campos
Summary: This study analyzed the toxic effects of Roundup Transorb (R) on the endangered Neotropical annual killifish Austrolebias charrua by evaluating molecular and biochemical biomarkers. The findings showed that exposure to Roundup significantly increased the production of reactive oxygen species, lipid peroxidation, and DNA damage in the fish's blood cells. It also decreased membrane fluidity. Gene expression analysis revealed alterations in genes associated with oxidative stress and antioxidant defense. This study provides new insights into adaptive mechanisms of A. charrua in response to Roundup, and it has important implications for environmental monitoring and aquatic toxicology assessment.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Xiaochan Gao, Hongtao Ren, Yong Huang, Yimin Li, Jiaqi Shen
Summary: The influence of multi-walled carbon nanotubes (MWCNTs) on the toxicity of zinc oxide nanoparticles (ZnONPs) in the intestine of common carp was investigated. The study found that the concentration and concentration ratio of the mixture played a role in the toxicity of ZnONPs. The presence of MWCNTs significantly decreased intercellular zinc accumulation and altered the microbial community diversity and composition of the gut microbiota.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Guangchao Yang, Qian Yang, Trust Beta, Qin Liu, Zhenzhu Zhu, Fei Shen
Summary: The study found that black garlic MLDs have alleviative effects on ZEN-induced toxicity, and exert their effects through antioxidative, anti-apoptotic, and inhibitory mechanisms on ZEN absorption.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Tuul Sepp, Ciara Baines, Randel Kreitsberg, Joern Peter Scharsack, Pedro Nogueira, Thomas Lang, Jerome Fort, Elin Sild, John T. Clarke, Arvo Tuvikene, Richard Meitern
Summary: Environmental factors can cause cancer in both wild animals and humans. The use of polluted habitats provides opportunities to study cancer defense mechanisms, which can be influenced by genetic variation and natural selection. Flatfish species, specifically flounders, show higher resistance to pollution-induced cancer compared to dabs, with elevated pollutant metabolism and stronger tumor suppression mechanisms in their liver tissue. This suggests a potential hormetic upregulation of tumor suppression or stronger natural selection pressure for cancer resistance in flounders. Wild species offer valuable insights into understanding the nature and evolution of natural cancer defense mechanisms.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Regan A. Moss, Kaitlyn M. Murphy, Steven T. Gardner, Madison M. Watkins, John W. Finger Finger Jr, Meghan D. Kelley, Ruth M. Elsey, Daniel A. Warner, Mary T. Mendonca
Summary: The study explored the impact of environmentally relevant concentrations of E2 on the immune system and hormone levels of hatchling American alligators. Results showed that exposure to environmental estrogen can alter plasma hormone concentrations but have little to no impact on morphology and immune responses.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ye Liang, Zihao Li, Jing Yuan, Yiwen Zhou, Meng Li, Haifeng Gu
Summary: This study investigated the toxicity mechanism of homo-YTX and NH3-N on the survival of abalone. The results showed that homo-YTX and NH3-N enhanced oxidative stress and lipid peroxidation reactions, inhibited energy supply, disrupted metabolic and immune physiological functions, and activated apoptosis in the gills of abalone.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)