Article
Chemistry, Analytical
Yan Yang, Yingai Yin, Sai Wang, Yiyang Dong
Summary: A bifunctional aptamer and MST-aptasensor were developed for simultaneous determination of ZEN and OTA, showing high affinity and specificity with a LOD of 0.12 nM and satisfactory recoveries. The bifunctional aptamer and MST-aptasensor have great potential in practical applications.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Zefeng Mao, Xiaojuan Wang, Ruipeng Chen, Zixuan Zhou, Shuyue Ren, Jun Liang, Zhixian Gao
Summary: The CRISPR-Cas system has made significant breakthroughs in the field of molecular diagnostics. In this study, a biosensing method powered by CRISPR/Cas12a was designed to detect ochratoxin A. Compared to traditional fluorescent probes, this method is faster and has comparable detection capabilities.
Article
Chemistry, Analytical
Yun Chen, Dehui Qiu, Xiaobo Zhang, Yuan Liu, Mingpan Cheng, Jianping Lei, Jean-Louis Mergny, Huangxian Ju, Jun Zhou
Summary: In this study, F3T-azaC-hemin, a G4-based construct, was designed to address the low activity issue of G-quadruplex/hemin (G4/hemin) DNAzymes. The performances of the G4-DNAzyme were significantly improved, resulting in a new and highly efficient molecular tool for ultrasensitive chemiluminescent detection of miRNA-221.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Tian Luan, Huan-Huan Yang, Meng-Yu Niu, Yin-He Zhao, Shou-Shan Chen, Lei Lv, Xuan-Jun Li, Zhi-Jun Guo
Summary: A label-free aptamer fluorescent sensor was developed for the rapid determination of Ochratoxin A (OTA). The method showed good selectivity and sensitivity, with lower cost compared to other aptamer-based OTA detection methods. It has promising application prospects in OTA determination.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Dandan Zhang, Xiangyue Cai, Qian Zhang, Chunyang Zhang
Summary: We developed a fluorescent aptasensor for label-free detection of ochratoxin A (OTA) based on TdT- and DNA polymerase-assisted multisite strand displacement amplification. This aptasensor exhibits good specificity and high sensitivity with a limit of detection (LOD) of 0.18 ng mL(-1), and it can be further applied for the accurate quantification of OTA in complex real samples, holding promising applications in the field of food safety.
CHEMICAL COMMUNICATIONS
(2023)
Review
Food Science & Technology
Elif Atay, Aylin Altan
Summary: Foodborne diseases caused by pathogen bacteria pose a serious threat to human life worldwide. Conventional methods for detecting these bacteria are inefficient and time-consuming. Biosensors offer a promising alternative for rapid and precise detection of foodborne pathogens. This review focuses on aptamer, nanofiber, and metal organic framework-based biosensors, discussing their advantages, limitations, and potential future developments.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Chemistry, Analytical
Futing Wang, Peng Wang, Hongfen Yang, Ren Cai, Weihong Tan
Summary: A self-powered biosensing system is designed for real-time detection of PDGF-BB concentration. The system utilizes HCR reaction, with a decrease in open-circuit voltage when PDGF-BB is present. The detection limit of the system is 0.031 pg/mL.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Futing Wang, Peng Wang, Hongfen Yang, Ren Cai, Weihong Tan
Summary: A self-powered biosensing system with multivariate signal amplification was developed for ultrasensitive and real-time detection of PDGF-BB. By utilizing the hybridization chain reaction, DNA amplification was achieved, leading to a change in the open-circuit voltage of the biosensing system, enabling the real-time detection of PDGF-BB. The detection limit of the system was 0.031 pg/mL.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Mengzhen Lv, Wan Zhou, Hamed Tavakoli, Cynthia Bautista, Jianfei Xia, Zonghua Wang, XiuJun Li
Summary: MOFs, as a class of crystalline porous materials, have been widely applied in various fields due to their nanoscale framework structure, adjustable pore size, large specific surface area, and good chemical stability. With the functionalization of aptamers, MOFs-based biosensors have shown great potential in medical diagnostics, food safety inspection, and environmental surveillance. Researchers are also exploring stimuli-responsive MOFs and the integration of MOFs on microfluidic devices for sensing applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Jiafeng Pan, Fang Deng, Zhi Liu, Gu Shi, Junhua Chen
Summary: In this work, an enzyme-free biosensor based on toehold-mediated catalytic hairpin assembly and DNAzyme-cascaded hydrolysis reaction is reported for mycotoxin detection. The sensor showed high sensitivity and selectivity, and was successfully applied for the quantification of mycotoxins in wine samples. Additionally, the platform was used to fabricate concatenated logic gates, providing a universal sensing strategy for the intelligent detection of multiplex mycotoxins.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Xiaojuan Zhang, Yun Gao, Bowen Deng, Bo Hu, Luming Zhao, Han Guo, Chengfang Yang, Zhenxia Ma, Mingjuan Sun, Binghua Jiao, Lianghua Wang
Summary: This study successfully obtained an aptamer with high affinity and specificity for the challenging marine biotoxin GYM-A through Capture-SELEX, and established a novel aptasensor based on BLI technology for the detection of GYM-A, showing excellent performance.
Article
Chemistry, Multidisciplinary
Qingbao Yang, Yanhua Qi, Jingming Zhou, Yumei Chen, Chao Liang, Zhanxiang Liu, Xiaoli Zhang, Aiping Wang
Summary: A novel fluorescent immunochromatographic assay based on quantum dots was developed for the detection of fleroxacin residues in pork meat, showing good accuracy and specificity.
Article
Chemistry, Analytical
Hongyuan Cui, Xueqi Fu, Lin Yang, Shu Xing, Xiao-Feng Wang
Summary: The fluorescence resonance energy transfer (FRET) aptasensor constructed with two-dimensional titanium carbides (MXenes) shows high sensitivity and specificity for quantitative detection of thrombin, with a detection limit of 5.27 pM. Among three different aptamers, HD22 exhibits the most sensitivity in detecting thrombin in human plasma, and the Ti3C2 MXene-based sensor can accurately detect thrombin in human serum. These results indicate a great potential of Ti3C2 MXene-based FRET aptasensor in clinical diagnosis.
Article
Chemistry, Analytical
Poonam Singh, Sandeep Kumar, Sanjay Kumar Verma
Summary: A fluorescent graphene oxide based aptasensing platform was developed for sensitive and accurate detection of acephate. The screened aptamer AAPT3 demonstrated high affinity and selectivity, and the aptasensor showed low detection limit and wide linear range.
Review
Environmental Sciences
Kamal Rahimizadeh, Qurat ul ain Zahra, Suxiang Chen, Bao T. Le, Ismat Ullah, Rakesh N. Veedu
Summary: Given the importance of public health, the development of quick, targeted, highly sensitive, and accurate technologies to monitor pathogenic microbes is crucial. Nanomaterial-conjugated aptasensors have advantages in improving sensitivity and specificity, and have potential applications in bioanalysis of food and environmental samples.
ENVIRONMENTAL RESEARCH
(2023)
Article
Food Science & Technology
Valentina Innamorato, Francesco Longobardi, Vincenzo Lippolis, Marina Cortese, Antonio F. Logrieco, Lucia Catucci, Angela Agostiano, Annalisa De Girolamo
FOOD ANALYTICAL METHODS
(2019)
Article
Agriculture, Multidisciplinary
Annalisa De Girolamo, Salvatore Cervellieri, Marina Cortese, Anna Chiara Raffaella Porricelli, Michelangelo Pascale, Francesco Longobardi, Christoph von Holst, Leonardo Ciaccheri, Vincenzo Lippolis
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2019)
Article
Food Science & Technology
Veronica M. T. Lattanzio, Christoph von Holst, Vincenzo Lippolis, Annalisa De Girolamo, Antonio F. Logrieco, Hans G. J. Mol, Michelangelo Pascale
Article
Chemistry, Applied
Annalisa De Girolamo, Christoph von Holst, Marina Cortese, Salvatore Cervellieri, Michelangelo Pascale, Francesco Longobardi, Lucia Catucci, Anna Chiara Raffaella Porricelli, Vincenzo Lippolis
Article
Chemistry, Applied
Hasim Munawar, Abeer H. M. Safaryan, Annalisa De Girolamo, Alvaro Garcia-Cruz, Pedro Marote, Kal Karim, Vincenzo Lippolis, Michelangelo Pascale, Sergey A. Piletsky
Article
Chemistry, Analytical
Michelangelo Pascale, Annalisa De Girolamo, Vincenzo Lippolis, Joerg Stroka, Hans G. J. Mol, Veronica M. T. Lattanzio
JOURNAL OF AOAC INTERNATIONAL
(2019)
Article
Food Science & Technology
Annalisa De Girolamo, Marina Cortese, Salvatore Cervellieri, Vincenzo Lippolis, Michelangelo Pascale, Antonio Francesco Logrieco, Michele Suman
Article
Food Science & Technology
Michelangelo Pascale, Antonio F. Logrieco, Matthias Graeber, Marina Hirschberger, Mareike Reichel, Vincenzo Lippolis, Annalisa De Girolamo, Veronica M. T. Lattanzio, Katarina Slettengren
Article
Food Science & Technology
Annalisa De Girolamo, Marcia Carolina Arroyo, Salvatore Cervellieri, Marina Cortese, Michelangelo Pascale, Antonio Francesco Logrieco, Vincenzo Lippolis
LWT-FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Chemistry, Applied
Annalisa De Girolamo, Marcia Carolina Arroyo, Vincenzo Lippolis, Salvatore Cervellieri, Marina Cortese, Michelangelo Pascale, Antonio Francesco Logrieco, Christoph von Holst
Article
Polymer Science
Fulvio Ciriaco, Vincenzo De Leo, Lucia Catucci, Michelangelo Pascale, Antonio F. Logrieco, Maria C. DeRosa, Annalisa De Girolamo
Article
Food Science & Technology
Annalisa De Girolamo, Biancamaria Ciasca, Michelangelo Pascale, Veronica M. T. Lattanzio
Review
Food Science & Technology
Annalisa De Girolamo, Vincenzo Lippolis, Michelangelo Pascale
Summary: This article reviews the relevant literature on liquid chromatography mass spectrometry methods for the analysis of various natural toxins in food. A total of 96 papers from 2011 to 2021 were selected for review. Different approaches in sample preparation, chromatographic separation, and detection mode are discussed. The article particularly focuses on the analytical performance characteristics obtained during the validation process and the application to real samples.
Article
Food Science & Technology
Michelangelo Pascale, Antonio F. Logrieco, Vincenzo Lippolis, Annalisa De Girolamo, Salvatore Cervellieri, Veronica M. T. Lattanzio, Biancamaria Ciasca, Anna Vega, Mareike Reichel, Matthias Graeber, Katarina Slettengren
Summary: This study evaluated the effectiveness of grain cleaning in reducing levels of co-occurring Fusarium toxins in maize at an industrial scale using mechanical and/or optical sorting technologies. Results showed that grain cleaning can significantly reduce contamination levels of DON, ZEA, and FBs in maize, with reduction rates of up to 2670%.
Article
Food Science & Technology
Annalisa De Girolamo, Salvatore Cervellieri, Erminia Mancini, Michelangelo Pascale, Antonio Francesco Logrieco, Vincenzo Lippolis