Review
Pharmacology & Pharmacy
Shunling Yuan, Guangfeng Li, Jinbo Zhang, Xiao Chen, Jiacan Su, Fengjin Zhou
Summary: Osteoarthritis (OA) is a degenerative disease characterized by cartilage damage and severe pain. Current therapies fail to halt OA progression, creating a need for alternative approaches. Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) offer potential as a regenerative treatment for OA, possessing anti-inflammatory properties and promoting cartilage healing. MSC-EVs have advantages over cell-based therapies, and researchers are exploring strategies to enhance their delivery and efficacy. This article reviews the potential use of MSC-EVs in treating OA, discussing benefits, challenges, and the possibility of using allogeneic and autologous MSC-EVs.
Article
Medicine, Research & Experimental
Huan Liu, Yuehong Chen, Geng Yin, Qibing Xie
Summary: Autoimmune diseases are chronic conditions with impaired self-tolerance, lacking effective treatment options. Mesenchymal stem cells release EVs and miRNAs that can modulate immune responses, offering potential therapeutic benefits but also facing challenges in clinical application.
Review
Biochemistry & Molecular Biology
Ying Zhu, Zhao-Fu Liao, Miao-Hua Mo, Xing-Dong Xiong
Summary: Extracellular vesicles (EVs), including mesenchymal stromal cell-derived EVs (MSC-EVs), play crucial roles in cell-cell communication and have potential therapeutic applications in various vascular disorders. MSC-EVs have shown beneficial effects on angiogenesis and different vasculopathies such as atherosclerosis and vascular restenosis. Moreover, modified MSC-EVs and suitable biomaterials can further enhance their therapeutic effects. This review provides an overview of the functional roles of MSC-EVs in maintaining vascular homeostasis and discusses strategies to optimize their therapeutic applications.
Review
Cell Biology
Gyorgyi Muzes, Ferenc Sipos
Summary: This article discusses the application of adult stem cell secretome in immune-mediated inflammatory diseases (IMIDs), highlighting its anti-inflammatory, anti-apoptotic, and immunomodulatory properties. While promising results have been obtained from pre-clinical studies, further research is needed in human studies.
Review
Biotechnology & Applied Microbiology
Behnaz Banimohamad-Shotorbani, Hekmat Farajpour, Farshid Sefat, Shiva Ahdi Khosroshahi, Hajar Shafaei, Saeed Heidari Keshel
Summary: After the outbreak of COVID-19, the use of mesenchymal stromal cells, extracellular vesicles, and secretome for treating respiratory diseases has shown promising and optimistic outcomes.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Medicine, Research & Experimental
Cassia Lisboa Braga, Renata Trabach Santos, Carla Medeiros da Silva, Nazareth de Novaes Rocha, Nathane Santanna Felix, Mayck Medeiros, Monique Martins Melo, Johnatas Dutra Silva, Douglas Esteves Teixeira, Celso Caruso Neves, Patricia Rieken Macedo Rocco, Fernanda Ferreira Cruz, Pedro Leme Silva
Summary: This study evaluated the preconditioning effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) and their extracellular vesicles (EVs) in experimental pulmonary arterial hypertension (PAH). The results showed that BM-MSCs and EVs preconditioned under normoxia or hypoxia were effective in reducing right ventricular pressure, perivascular collagen content, and vascular wall thickness in PAH rats. Additionally, they also played a positive role in improving endothelial to mesenchymal transition.
Review
Cell & Tissue Engineering
Ali Rajool Dezfuly, Azadeh Safaee, Hossein Salehi
Summary: Extracellular vesicles (EVs), including microvesicles and exosomes, carry vital information such as lipids, proteins, mRNAs, and microRNAs to target cells. Studies have shown that the therapeutic effects of stem cells are attributed to the miRNAs in their EVs, which may help in developing new therapeutic techniques for retinal degenerative diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Pharmacology & Pharmacy
Xinwen Wang, Haodong Tian, Xinquan Yang, Hongmou Zhao, Xiaojun Liang, Yi Li
Summary: Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have been proposed as promising alternatives to traditional cell-based therapeutics in clinical settings. This study aims to systematically review the current knowledge of MSC-EVs and highlight their potential application in treating orthopedic diseases, such as bone defects, osteoarthritis, osteoporosis, intervertebral disc degeneration, osteosarcoma, and osteoradionecrosis.
ADVANCED THERAPEUTICS
(2023)
Review
Cell Biology
Jancy Johnson, Mozhgan Shojaee, James Mitchell Crow, Ramin Khanabdali
Summary: MSCs, as a leading cell source for stem cell therapy, have regenerative and immunomodulatory properties, but face challenges in clinical applications. EVs and EEVs, as potential cell-free alternatives, may overcome these challenges in the clinical application of MSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Mahmoud Kandeel, Mohamed A. Morsy, Khalid M. Alkhodair, Sameer Alhojaily
Summary: This article examines the potential therapeutic applications of MSC-derived EVs in addressing neurodegenerative disorders such as Alzheimer's disease, multiple sclerosis, Parkinson's disease, amyotrophic lateral sclerosis, and Huntington's disease. It also discusses the present state-of-the-art for MSC-EV-based therapy in these diseases. EVs offer a viable cell-free therapy alternative for tissue regeneration and repair, and recent research has enhanced their content and applicability.
Review
Biotechnology & Applied Microbiology
Martina Sandona, Lorena Di Pietro, Federica Esposito, Alessia Ventura, Antonietta Rosa Silini, Ornella Parolini, Valentina Saccone
Summary: Mesenchymal stromal cells (MSCs) are multipotent cells found in various tissues that have anti-fibrotic and immunoregulatory properties. Their secretome, including extracellular vesicles (EVs), can mimic the therapeutic effects of MSCs and offer advantages such as easier storage and safer administration for muscle regeneration in different diseases.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Noelia Munoz-Dominguez, Santiago Roura, Cristina Prat-Vidal, Joaquim Vives
Summary: Significant progress has been made in developing therapeutic options for myocardial infarction patients, but current treatments only partially address the functional recovery of post-infarcted myocardium. Novel cell and tissue engineering therapeutic approaches, particularly using MSC and natural extracellular matrices, as well as MSC-derived EV, show promise in cardiac repair for post-MI management.
Article
Cell & Tissue Engineering
Kevin Las Heras, Felix Royo, Clara Garcia-Vallicrosa, Manoli Igartua, Edorta Santos-Vizcaino, Juan M. Falcon-Perez, Rosa Maria Hernandez
Summary: This study demonstrates that EVs derived from hair follicle-derived MSCs (HF-MSCs) have comparable potential to adipose tissue-derived MSCs (AT-MSCs) in the treatment of chronic wounds.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Zi-Yi Feng, Shu-De Yang, Ting Wang, Shu Guo
Summary: Melatonin, synthesized in the pineal gland, controls the body's circadian rhythm and has various effects including regulating metabolism, providing neuroprotection, and acting as an anti-inflammatory and free radical scavenger. Research has shown that melatonin can play a role in regulating MSCs, which have the potential for tissue regeneration through paracrine signaling. MSC-derived extracellular vesicles (EVs) could potentially offer advantages over MSCs in regenerative medicine due to their ability to respond to injury and promote tissue regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Dimitrios Tsiapalis, Achilleas Floudas, Tobias Tertel, Verena Boerger, Bernd Giebel, Douglas J. Veale, Ursula Fearon, Lorraine O'Driscoll
Summary: This study investigated the effect of human mesenchymal/stromal stem cells and their extracellular vesicles (EVs) on CD4+ T cells from rheumatoid arthritis (RA) patients. The researchers found that EVs from IFN-beta-primed MSCs had a stronger immune-regulatory effect on RA CD4+ T cells compared to MSCs or EVs from naive MSCs. In addition, EVs from IFN-beta-primed MSCs effectively inhibited RA FLS migration and downregulated RA FLS surface markers. These findings suggest that EVs from IFN-beta-primed MSCs could be a potential therapy for RA.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Anesthesiology
Christoph Boesing, Laura Schaefer, Marvin Hammel, Mirko Otto, Susanne Blank, Paolo Pelosi, Patricia R. M. Rocco, Thomas Luecke, Joerg Krebs
Summary: This study compared the end-expiratory transpulmonary pressure between fixed and individualized positive end-expiratory pressure (PEEP) strategies in superobese patients and analyzed their effects on respiratory mechanics, end-expiratory lung volume, gas exchange, and hemodynamic parameters. The study found that individualized PEEP strategy resulted in lower end-expiratory transpulmonary pressure while improving respiratory mechanics, lung volume, and oxygenation. Therefore, individualized PEEP strategy may be a feasible approach for respiratory support in superobese patients undergoing laparoscopic surgery.
Article
Biotechnology & Applied Microbiology
Chirag M. Vaswani, Amir K. Varkouhi, Sahil Gupta, Amin M. Ektesabi, James N. Tsoporis, Sadiya Yousef, Pamela J. Plant, Adriana L. da Silva, Yuchen Cen, Yi-Chieh Tseng, Sabrina S. Batah, Alexandre T. Fabro, Suzanne L. Advani, Andrew Advani, Howard Leong-Poi, John C. Marshall, Cristiana C. Garcia, Patricia R. M. Rocco, Guillermo M. Albaiceta, Steffen Sebastian-Bolz, Tania H. Watts, Theo J. Moraes, Vera L. Capelozzi, Claudia. C. dos Santos
Summary: Virus-induced lung injury is associated with loss of pulmonary epithelial-endothelial tight junction integrity. The influenza virus exploits miR-193b-5p to target occludin and evade the host antiviral defense system. Inhibiting miR-193b-5p enhances antiviral responses, reduces lung injury, and improves survival.
Article
Critical Care Medicine
Denise Battaglini, Elena Ciaravolo, Salvatore Caiffa, Lara Delpiano, Lorenzo Ball, Antonio Vena, Daniele R. Giacobbe, Matteo Bassetti, Basil Matta, Paolo Pelosi, Chiara Robba
Summary: This study evaluated the effects of physiotherapy on respiratory and cerebral function in mechanically ventilated patients. The results showed that physiotherapy improved gas exchange in COVID-19 patients and cerebral oxygenation in non-COVID-19 patients. This indicates that physiotherapy is beneficial for functional improvement and rehabilitation in mechanically ventilated patients.
Article
Physiology
Gustavo R. Zubieta-Calleja, Natalia Zubieta-DeUrioste, Felipe de Jesus Montelongo, Manuel Gabriel Romo Sanchez, Aurio Fajardo Campoverdi, Patricia Rieken Macedo Rocco, Denise Battaglini, Lorenzo Ball, Paolo Pelosi
Summary: COVID-19 can severely affect respiratory function and result in life-threatening hypoxia. Understanding the histopathological and functional patterns is crucial for understanding the pathophysiology of COVID-19 and developing new therapeutic strategies. Patients with COVID-19 may have different respiratory stages, which are associated with the extent of lung involvement and can affect ventilation and perfusion relationships.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2023)
Article
Physiology
Carla M. Silva, Debora S. Ornellas, Felipe M. Ornellas, Raquel S. Santos, Sabrina V. Martini, Debora Ferreira, Caroline Muiler, Fernanda F. Cruz, Christina M. Takiya, Patricia R. M. Rocco, Marcelo M. Morales, Pedro L. Silva
Summary: This study evaluated the early effects of bone marrow-derived mononuclear cell (BMDMC) therapy on lung and kidney in experimental polymicrobial sepsis. The results showed that BMDMC therapy can reduce lung and kidney damage in a time-dependent manner. Therefore, BMDMCs may represent a potential therapy for sepsis induction.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2023)
Biographical-Item
Critical Care Medicine
Patricia R. M. Rocco
ANNALS OF INTENSIVE CARE
(2023)
Review
Pharmacology & Pharmacy
Miqueias Lopes-Pacheco, Patricia R. M. Rocco
Summary: Respiratory diseases pose significant health concerns worldwide, with high morbidity and mortality rates and considerable burdens on patients. Despite advances in understanding the pathologic mechanisms, current therapies for severe respiratory diseases are predominantly supportive and cannot improve lung function or reverse tissue remodeling. Mesenchymal stromal cells (MSCs) have shown promise in promoting immunomodulation, tissue repair, and other therapeutic properties in experimental models, but have had limited efficacy in early-stage clinical trials. Functional enhancement strategies, such as genetic engineering and preconditioning methods, have emerged to enhance the therapeutic actions of MSCs and improve clinical outcomes. This review discusses various strategies investigated in experimental settings to potentiate the therapeutic properties of MSCs for respiratory diseases, including changes in culture conditions, exposure to inflammatory environments, pharmacological agents, and genetic manipulation.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Cell Biology
Debora P. Ferreira, Sabrina V. Martini, Helena A. Oliveira, Adriana L. Silva, Siddharth Shenoy, Daiqin Chen, Valentina Simon, Eric Han, Natalie E. West, Jung Soo Suk, Patricia R. M. Rocco, Hilda Petrs-Silva, Marcelo M. Morales, Fernanda F. Cruz
Summary: This study successfully demonstrated the effective delivery of gene therapy to the lung using a mutant AAV8 vector, which showed high efficiency in penetrating mucus. The transfer of the PEDF gene did not have any adverse effects on lung parameters, providing a basis for further research on PEDF gene therapy.
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Anesthesiology
Denise Battaglini, Lara Delpiano, Denise Masuello, Pedro Leme Silva, Patricia R. M. Rocco, Basil Matta, Paolo Pelosi, Chiara Robba
Summary: The aim of this study was to evaluate the effects of PEEP augmentation test on brain oxygenation, systemic oxygen cascade, and metabolism in patients with acute brain injury. The results showed that PEEP augmentation test did not affect cerebral oxygenation, systemic oxygen cascade parameters, and metabolism.
JOURNAL OF CLINICAL MONITORING AND COMPUTING
(2023)
Meeting Abstract
Critical Care Medicine
C. C. Araujo, R. S. Santos, N. N. Rocha, N. S. Felix, V. L. Capelozzi, G. Cipriano Junior, P. L. Silva, P. R. M. Rocco
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Critical Care Medicine
John J. J. Marini, Lauren T. T. Thornton, Patricia R. M. Rocco, Luciano Gattinoni, Philip S. S. Crooke
Summary: Assessing the risk of ventilator-induced lung injury (VILI) at the bedside requires parameters that can be easily measured. Driving pressure (DP) and end-inspiratory static 'plateau' pressure (P-s) are useful in this regard, but lack key information about associated volume changes and cumulative strain. 'Mechanical power', a comprehensive variable that accounts for ventilating frequency and energy cost per tidal cycle, has been suggested as a useful concept. However, the absolute levels of energy and power alone may not provide precise enough information for safe ventilatory practice.
Article
Critical Care Medicine
Lavienraj Premraj, Christopher Camarda, Nicole White, Daniel Agustin Godoy, Brian H. Cuthbertson, Patricia R. M. Rocco, Paolo Pelosi, Chiara Robba, Jose I. Suarez, Sung-Min Cho, Denise Battaglini
Summary: This meta-analysis investigated the association between the timing of tracheostomy and mortality, neurological outcomes, and ICU/hospital length of stay in stroke patients. The results showed that the timing of tracheostomy was not associated with these outcomes.
Review
Critical Care Medicine
Pedro Leme Silva, Martin Scharffenberg, Patricia Rieken Macedo Rocco
Summary: Mechanical ventilation is a life-saving therapy in clinical situations, but it can also cause lung injury. This review discusses the mechanisms of ventilator-induced lung injury (VILI) and strategies for mitigating it.
INTENSIVE CARE MEDICINE EXPERIMENTAL
(2023)
Review
Pharmacology & Pharmacy
Denise Battaglini, Ida Giorgia Iavarone, Lou'i Al-Husinat, Lorenzo Ball, Chiara Robba, Pedro Leme Silva, Fernanda F. Cruz, Patricia R. M. Rocco
Summary: Despite progress in pharmacotherapy, anti-inflammatory strategies for ARDS treatment remain controversial. However, personalized medicine may be a promising approach for identifying specific subgroups that may benefit from anti-inflammatory treatment.
EXPERT OPINION ON INVESTIGATIONAL DRUGS
(2023)
Article
Critical Care Medicine
Christoph Boesing, Laura Schaefer, Peter T. Graf, Paolo Pelosi, Patricia R. M. Rocco, Thomas Luecke, Joerg Krebs
Summary: In ARDS patients treated with VV ECMO, adjusting PEEP to 10 cmH(2)O can reduce MP, improve hemodynamic parameters, and increase oxygen delivery.
JOURNAL OF CRITICAL CARE
(2024)
