Review
Pharmacology & Pharmacy
Shunling Yuan, Guangfeng Li, Jinbo Zhang, Xiao Chen, Jiacan Su, Fengjin Zhou
Summary: Osteoarthritis (OA) is a degenerative disease characterized by cartilage damage and severe pain. Current therapies fail to halt OA progression, creating a need for alternative approaches. Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) offer potential as a regenerative treatment for OA, possessing anti-inflammatory properties and promoting cartilage healing. MSC-EVs have advantages over cell-based therapies, and researchers are exploring strategies to enhance their delivery and efficacy. This article reviews the potential use of MSC-EVs in treating OA, discussing benefits, challenges, and the possibility of using allogeneic and autologous MSC-EVs.
Article
Medicine, Research & Experimental
Huan Liu, Yuehong Chen, Geng Yin, Qibing Xie
Summary: Autoimmune diseases are chronic conditions with impaired self-tolerance, lacking effective treatment options. Mesenchymal stem cells release EVs and miRNAs that can modulate immune responses, offering potential therapeutic benefits but also facing challenges in clinical application.
Review
Biochemistry & Molecular Biology
Ying Zhu, Zhao-Fu Liao, Miao-Hua Mo, Xing-Dong Xiong
Summary: Extracellular vesicles (EVs), including mesenchymal stromal cell-derived EVs (MSC-EVs), play crucial roles in cell-cell communication and have potential therapeutic applications in various vascular disorders. MSC-EVs have shown beneficial effects on angiogenesis and different vasculopathies such as atherosclerosis and vascular restenosis. Moreover, modified MSC-EVs and suitable biomaterials can further enhance their therapeutic effects. This review provides an overview of the functional roles of MSC-EVs in maintaining vascular homeostasis and discusses strategies to optimize their therapeutic applications.
Review
Cell Biology
Gyorgyi Muzes, Ferenc Sipos
Summary: This article discusses the application of adult stem cell secretome in immune-mediated inflammatory diseases (IMIDs), highlighting its anti-inflammatory, anti-apoptotic, and immunomodulatory properties. While promising results have been obtained from pre-clinical studies, further research is needed in human studies.
Review
Biotechnology & Applied Microbiology
Behnaz Banimohamad-Shotorbani, Hekmat Farajpour, Farshid Sefat, Shiva Ahdi Khosroshahi, Hajar Shafaei, Saeed Heidari Keshel
Summary: After the outbreak of COVID-19, the use of mesenchymal stromal cells, extracellular vesicles, and secretome for treating respiratory diseases has shown promising and optimistic outcomes.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Medicine, Research & Experimental
Cassia Lisboa Braga, Renata Trabach Santos, Carla Medeiros da Silva, Nazareth de Novaes Rocha, Nathane Santanna Felix, Mayck Medeiros, Monique Martins Melo, Johnatas Dutra Silva, Douglas Esteves Teixeira, Celso Caruso Neves, Patricia Rieken Macedo Rocco, Fernanda Ferreira Cruz, Pedro Leme Silva
Summary: This study evaluated the preconditioning effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) and their extracellular vesicles (EVs) in experimental pulmonary arterial hypertension (PAH). The results showed that BM-MSCs and EVs preconditioned under normoxia or hypoxia were effective in reducing right ventricular pressure, perivascular collagen content, and vascular wall thickness in PAH rats. Additionally, they also played a positive role in improving endothelial to mesenchymal transition.
Review
Cell & Tissue Engineering
Ali Rajool Dezfuly, Azadeh Safaee, Hossein Salehi
Summary: Extracellular vesicles (EVs), including microvesicles and exosomes, carry vital information such as lipids, proteins, mRNAs, and microRNAs to target cells. Studies have shown that the therapeutic effects of stem cells are attributed to the miRNAs in their EVs, which may help in developing new therapeutic techniques for retinal degenerative diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Pharmacology & Pharmacy
Xinwen Wang, Haodong Tian, Xinquan Yang, Hongmou Zhao, Xiaojun Liang, Yi Li
Summary: Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have been proposed as promising alternatives to traditional cell-based therapeutics in clinical settings. This study aims to systematically review the current knowledge of MSC-EVs and highlight their potential application in treating orthopedic diseases, such as bone defects, osteoarthritis, osteoporosis, intervertebral disc degeneration, osteosarcoma, and osteoradionecrosis.
ADVANCED THERAPEUTICS
(2023)
Review
Cell Biology
Jancy Johnson, Mozhgan Shojaee, James Mitchell Crow, Ramin Khanabdali
Summary: MSCs, as a leading cell source for stem cell therapy, have regenerative and immunomodulatory properties, but face challenges in clinical applications. EVs and EEVs, as potential cell-free alternatives, may overcome these challenges in the clinical application of MSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Mahmoud Kandeel, Mohamed A. Morsy, Khalid M. Alkhodair, Sameer Alhojaily
Summary: This article examines the potential therapeutic applications of MSC-derived EVs in addressing neurodegenerative disorders such as Alzheimer's disease, multiple sclerosis, Parkinson's disease, amyotrophic lateral sclerosis, and Huntington's disease. It also discusses the present state-of-the-art for MSC-EV-based therapy in these diseases. EVs offer a viable cell-free therapy alternative for tissue regeneration and repair, and recent research has enhanced their content and applicability.
Review
Biotechnology & Applied Microbiology
Martina Sandona, Lorena Di Pietro, Federica Esposito, Alessia Ventura, Antonietta Rosa Silini, Ornella Parolini, Valentina Saccone
Summary: Mesenchymal stromal cells (MSCs) are multipotent cells found in various tissues that have anti-fibrotic and immunoregulatory properties. Their secretome, including extracellular vesicles (EVs), can mimic the therapeutic effects of MSCs and offer advantages such as easier storage and safer administration for muscle regeneration in different diseases.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Noelia Munoz-Dominguez, Santiago Roura, Cristina Prat-Vidal, Joaquim Vives
Summary: Significant progress has been made in developing therapeutic options for myocardial infarction patients, but current treatments only partially address the functional recovery of post-infarcted myocardium. Novel cell and tissue engineering therapeutic approaches, particularly using MSC and natural extracellular matrices, as well as MSC-derived EV, show promise in cardiac repair for post-MI management.
Article
Cell & Tissue Engineering
Kevin Las Heras, Felix Royo, Clara Garcia-Vallicrosa, Manoli Igartua, Edorta Santos-Vizcaino, Juan M. Falcon-Perez, Rosa Maria Hernandez
Summary: This study demonstrates that EVs derived from hair follicle-derived MSCs (HF-MSCs) have comparable potential to adipose tissue-derived MSCs (AT-MSCs) in the treatment of chronic wounds.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Zi-Yi Feng, Shu-De Yang, Ting Wang, Shu Guo
Summary: Melatonin, synthesized in the pineal gland, controls the body's circadian rhythm and has various effects including regulating metabolism, providing neuroprotection, and acting as an anti-inflammatory and free radical scavenger. Research has shown that melatonin can play a role in regulating MSCs, which have the potential for tissue regeneration through paracrine signaling. MSC-derived extracellular vesicles (EVs) could potentially offer advantages over MSCs in regenerative medicine due to their ability to respond to injury and promote tissue regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Dimitrios Tsiapalis, Achilleas Floudas, Tobias Tertel, Verena Boerger, Bernd Giebel, Douglas J. Veale, Ursula Fearon, Lorraine O'Driscoll
Summary: This study investigated the effect of human mesenchymal/stromal stem cells and their extracellular vesicles (EVs) on CD4+ T cells from rheumatoid arthritis (RA) patients. The researchers found that EVs from IFN-beta-primed MSCs had a stronger immune-regulatory effect on RA CD4+ T cells compared to MSCs or EVs from naive MSCs. In addition, EVs from IFN-beta-primed MSCs effectively inhibited RA FLS migration and downregulated RA FLS surface markers. These findings suggest that EVs from IFN-beta-primed MSCs could be a potential therapy for RA.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Luisa Helena Andrade da Silva, Juliana Borges Vieira, Marianna Ribeiro Cabral, Mariana Alves Antunes, Daiheon Lee, Fernanda Ferreira Cruz, Justin Hanes, Patricia Rieken Macedo Rocco, Marcelo Marcos Morales, Jung Soo Suk
Summary: Silicosis is a lung disease caused by inhalation of silica dust, and millions of industrial workers worldwide are affected. A drug called nintedanib has shown potential in treating silicosis, but its oral formulation often leads to significant side effects. Researchers have developed a nanocrystal-based suspension of nintedanib for localized treatment of silicosis via inhalation, which has shown robust anti-fibrotic effects without notable adverse effects.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Anesthesiology
Paulo Henrique Xavier, Ana Carolina Fernandes Fonseca, Leonardo Alves Goncalves, Giselle Cavalho de Sousa, Mariana Coelho da Silva, Raquel Ferreira de Magalhaes Sacramento, Cynthia dos Santos Samary, Mayck Medeiros, Fernanda Ferreira Cruz, Vera Luiza Capelozzi, Nathane Satanna Felix, Paolo Pelosi, John J. Marini, Patricia Rieken Macedo Rocco, Pedro Leme Silva
Summary: This study found that gradually increasing respiratory rate can mitigate ventilation-induced lung injury in rats with mild acute respiratory distress syndrome. In addition, using a recruitment maneuver can prevent the injurious biologic impact of abrupt increases in respiratory rate.
Article
Cell Biology
Francisca Alcayaga-Miranda, Johnatas Dutra Silva, Nicol Parada, Luisa Helena Andrade da Silva, Fernanda Ferreira Cruz, Yildy Utreras, Yessia Hidalgo, Maria Ignacia Cadiz, Rafael Tapia Limonchi, Francisco Espinoza, Alejandro Bruhn, Maroun Khoury, Patricia R. M. Rocco, Jimena Cuenca
Summary: This study aimed to investigate the impact of cryopreserved MenSCs on ARDS and determine the optimal therapeutic dose, safety, and efficacy. The results showed that clinical-grade cryo-MenSCs can improve lung function, reduce alveolar collapse and tissue cellularity, and modulate inflammatory mediators, promoting angiogenesis and anti-apoptotic effects. The optimal therapeutic dose of 4x10(6) cells/kg was safe and effective, favoring improved lung function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Natalia G. Blanco, Natalia M. Machado, Ligia L. L. Castro, Mariana A. A. Antunes, Christina M. M. Takiya, Monique R. O. Trugilho, Luana R. R. Silva, Adriana F. Paes F. Leme, Romenia R. Domingues, Bianca A. A. Pauletti, Beatriz T. T. Miranda, Johnatas D. D. Silva, Claudia C. C. dos Santos, Pedro L. L. Silva, Patricia R. M. Rocco, Fernanda F. F. Cruz
Summary: The effects of different sources of MSC-derived extracellular vesicles (EVs), including those obtained from bone marrow (BM), adipose (AD), or lung (L) tissues, on sepsis were investigated. The proteome analysis of EVs revealed that BM-EVs were associated with less organ damage compared with AD-EVs and L-EVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Chirag M. Vaswani, Amir K. Varkouhi, Sahil Gupta, Amin M. Ektesabi, James N. Tsoporis, Sadiya Yousef, Pamela J. Plant, Adriana L. da Silva, Yuchen Cen, Yi-Chieh Tseng, Sabrina S. Batah, Alexandre T. Fabro, Suzanne L. Advani, Andrew Advani, Howard Leong-Poi, John C. Marshall, Cristiana C. Garcia, Patricia R. M. Rocco, Guillermo M. Albaiceta, Steffen Sebastian-Bolz, Tania H. Watts, Theo J. Moraes, Vera L. Capelozzi, Claudia. C. dos Santos
Summary: Virus-induced lung injury is associated with loss of pulmonary epithelial-endothelial tight junction integrity. The influenza virus exploits miR-193b-5p to target occludin and evade the host antiviral defense system. Inhibiting miR-193b-5p enhances antiviral responses, reduces lung injury, and improves survival.
Article
Critical Care Medicine
Denise Battaglini, Elena Ciaravolo, Salvatore Caiffa, Lara Delpiano, Lorenzo Ball, Antonio Vena, Daniele R. Giacobbe, Matteo Bassetti, Basil Matta, Paolo Pelosi, Chiara Robba
Summary: This study evaluated the effects of physiotherapy on respiratory and cerebral function in mechanically ventilated patients. The results showed that physiotherapy improved gas exchange in COVID-19 patients and cerebral oxygenation in non-COVID-19 patients. This indicates that physiotherapy is beneficial for functional improvement and rehabilitation in mechanically ventilated patients.
Article
Medicine, General & Internal
Pedro L. Silva, Fernanda F. Cruz, Camila M. Martins, Jacob Herrmann, Sarah E. Gerard, Yi Xin, Maurizio Cereda, Lorenzo Ball, Paolo Pelosi, Patricia R. M. Rocco
Summary: Lung weight can be measured with quantitative chest CT to evaluate the severity of pulmonary edema and prognosis in patients with COVID-19. Certain laboratory markers, such as leukocytes, neutrophils, D-dimer, and LDH, are found to be higher in patients with overweight lungs.
FRONTIERS IN MEDICINE
(2023)
Article
Medicine, Research & Experimental
Cassia Lisboa Braga, Renata Trabach Santos, Carla Medeiros da Silva, Nazareth de Novaes Rocha, Nathane Santanna Felix, Mayck Medeiros, Monique Martins Melo, Johnatas Dutra Silva, Douglas Esteves Teixeira, Celso Caruso Neves, Patricia Rieken Macedo Rocco, Fernanda Ferreira Cruz, Pedro Leme Silva
Summary: This study evaluated the preconditioning effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) and their extracellular vesicles (EVs) in experimental pulmonary arterial hypertension (PAH). The results showed that BM-MSCs and EVs preconditioned under normoxia or hypoxia were effective in reducing right ventricular pressure, perivascular collagen content, and vascular wall thickness in PAH rats. Additionally, they also played a positive role in improving endothelial to mesenchymal transition.
Article
Medicine, General & Internal
Taina B. Oliveira, Cassia L. Braga, Denise Battaglini, Paolo Pelosi, Patricia R. M. Rocco, Pedro L. Silva, Fernanda F. Cruz
Summary: This study compared the effects of sevoflurane and propofol on immune responses and cytokine expression in rats with sepsis. The results showed that sevoflurane increased the expression of anti-inflammatory biomarkers in monocytes, enhanced their phagocytic capacity, and increased neutrophil migration. Sevoflurane also protected lung epithelial and endothelial cells. in contrast, propofol had less significant effects.
FRONTIERS IN MEDICINE
(2023)
Review
Critical Care Medicine
Eduardo Butturini de Carvalho, Denise Battaglini, Chiara Robba, Manu L. N. G. Malbrain, Paolo Pelosi, Patricia Rieken Macedo Rocco, Pedro Leme Silva
Summary: Ventilatory management can be influenced by restrictive and liberal fluid strategies, potentially leading to damage to distal organs in critically ill patients. Pre-clinical studies have assessed the effects of different fluid strategies on ventilator-induced lung injury, both during and after changes in positive end-expiratory pressure (PEEP). Patients on mechanical ventilation can receive intravenous fluids through either restrictive or liberal fluid management. However, there is a lack of clear differentiation between these two strategies in the literature. Restrictive fluid management may result in hypoperfusion and distal organ damage, while liberal fluid strategy can cause various complications such as endothelial shear stress, glycocalyx damage, cardiovascular issues, lung edema, and distal organ dysfunction. The combination of liberal fluids and pressure-support ventilation, but not pressure control ventilation, may lead to further lung damage. This narrative review aims to provide a better understanding of the interaction between different fluid and ventilation strategies and their impact on the lungs and distal organs. The weaning phase of mechanical ventilation and the deresuscitation phase are not covered in this review.
INTENSIVE CARE MEDICINE EXPERIMENTAL
(2023)
Meeting Abstract
Critical Care Medicine
A. L. Silva, C. M. Bessa, N. D. Rocha, R. F. Magalhaes, E. B. Carvalho, C. Robba, P. Pelosi, C. D. Samary, P. R. Rocco, P. L. Silva
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Meeting Abstract
Critical Care Medicine
P. L. Silva, C. L. Braga, R. T. Trabach, S. S. D. Serra, N. D. Rocha, S. C. Mandacaru, M. R. D. Trugilho, F. F. Cruz, P. R. Rocco
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Meeting Abstract
Critical Care Medicine
C. C. Araujo, R. S. Santos, N. N. Rocha, N. S. Felix, V. L. Capelozzi, G. Cipriano Junior, P. L. Silva, P. R. M. Rocco
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Critical Care Medicine
John J. J. Marini, Lauren T. T. Thornton, Patricia R. M. Rocco, Luciano Gattinoni, Philip S. S. Crooke
Summary: Assessing the risk of ventilator-induced lung injury (VILI) at the bedside requires parameters that can be easily measured. Driving pressure (DP) and end-inspiratory static 'plateau' pressure (P-s) are useful in this regard, but lack key information about associated volume changes and cumulative strain. 'Mechanical power', a comprehensive variable that accounts for ventilating frequency and energy cost per tidal cycle, has been suggested as a useful concept. However, the absolute levels of energy and power alone may not provide precise enough information for safe ventilatory practice.
Article
Critical Care Medicine
Christoph Boesing, Laura Schaefer, Peter T. Graf, Paolo Pelosi, Patricia R. M. Rocco, Thomas Luecke, Joerg Krebs
Summary: In ARDS patients treated with VV ECMO, adjusting PEEP to 10 cmH(2)O can reduce MP, improve hemodynamic parameters, and increase oxygen delivery.
JOURNAL OF CRITICAL CARE
(2024)
