Article
Biochemical Research Methods
Chaochao Chen, Steven R. Laviolette, Shawn N. Whitehead, Justin B. Renaud, Ken K-C Yeung
Summary: In this study, zinc oxide nanoparticles were investigated as an alternative to titanium dioxide nanoparticles for laser desorption/ionization mass spectrometric imaging of low molecular weight molecules. Zinc oxide nanoparticles dissolved in acidic pH showed similar or better results compared to titanium dioxide nanoparticles in imaging LMWM, with regular acid washes effective in maintaining reproducibility. High-quality images of tissue sections were obtained, predominantly detecting ions as Na+ or K+ adducts in the positive ion mode, with ZnO NP detecting a similar number of LMWM as TiO2 NP.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Nhu Q. Vu, Amanda R. Buchberger, Jillian Johnson, Lingjun Li
Summary: Neuropeptides are low abundance signaling molecules that modulate physiological processes, and mass spectrometry imaging is increasingly useful in studying them. Proper tissue washes can increase detectable neuropeptides, and using a salt solution wash may affect neuropeptide detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Taiga Iwama, Kuniyuki Kano, Daisuke Saigusa, Kim Ekroos, Gerhild van Echten-Deckert, Johannes Vogt, Junken Aoki
Summary: The novel on-tissue derivatization method using Phos-tag in combination with MALDI-MSI enables visualization of low-abundance bioactive lipids like LPA and S1P in the brain, providing a simple and optimal way to reveal the spatial localization of these important molecules in tissues.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Weiwei Tang, Jun-Jie Shi, Wei Liu, Xu Lu, Bin Li
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) assisted genome mining strategy was applied to discover a glycosyltransferase (GT) named PgGT1 from the root of Platycodon grandiflorum. PgGT1 was found to catalyze the synthesis of platycoside E (PE) by sequentially attaching two beta-1,6-linked glucosyl residues to the glucosyl residue at the C3 position of platycodin D (PD). The study also revealed the crucial roles of residues S273, E274, and H350 in stabilizing the sugar donor and positioning the glucose for optimal glycosylation. These findings provide insights into the biosynthetic pathway of PE and have potential implications in industrial biotransformation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biochemical Research Methods
Jessica L. Moore, Georgia Charkoftaki
Summary: This review discusses recent advances in imaging mass spectrometry, specifically matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) applied on tissues. It explores sample preparation strategies, considerations for different analyte classes, and the use of multimodal imaging strategies. The review also examines experimental design approaches, standardization of processes, and data analysis platforms. It concludes with applications of imaging mass spectrometry in various fields, with a focus on medical research.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Hyun Jun Jang, Kyoung Ja Kwon, Chan Young Shin, Ga Seul Lee, Jeong Hee Moon, Tae Geol Lee, Sohee Yoon
Summary: Autism is associated with changes in polyunsaturated fatty acid (PUFA) levels, suggesting a link between lipid metabolism and autism spectrum disorder (ASD). In this study, phospholipid distribution in the brain tissue of autistic mice was investigated using MALDI-MSI, revealing differences in phospholipids containing important PUFAs for cell signaling and brain growth. The identification of these differences will contribute to a better understanding of changes in lipid metabolism in ASD, when combined with traditional biological fluid analysis.
Review
Endocrinology & Metabolism
Laura K. Schnackenberg, David A. Thorn, Dustyn Barnette, E. Ellen Jones
Summary: Neurological diseases pose a significant public health threat, necessitating research into improved early detection and more effective treatment approaches. MALDI IMS, as a label-free and innovative technique, has the potential to detect the spatial distribution of hundreds of analytes, showcasing its advantages in neurology and disease research.
METABOLIC BRAIN DISEASE
(2022)
Review
Chemistry, Multidisciplinary
Jin-jun Hou, Zi-jia Zhang, Wen-yong Wu, Qing-qing He, Teng-qian Zhang, Ya-wen Liu, Zhao-jun Wang, Lei Gao, Hua-li Long, Min Lei, Wan-ying Wu, De-an Guo
Summary: Natural products and their structural analogs play a major role in novel drug development, but few molecular imaging technologies can detect multiple compounds simultaneously. Mass spectrometry imaging has been instrumental in discovering natural products and evaluating their applications, providing a visualization tool for drug development.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Microbiology
Marceau Levasseur, Teo Hebra, Nicolas Elie, Vincent Guerineau, David Touboul, Veronique Eparvier
Summary: In this study, we proposed a workflow using a standardized protein and lipid extraction protocol and reliable spectral comparison algorithm in MetGem software for classification and identification of environmental microorganisms. Our method was validated and shown to be capable of classifying bacteria at the order and genus level, and fungi at the order level.
Article
Chemistry, Medicinal
Andreas Traberg, Fernanda E. Pinto, Anders C. N. Hansen, Merete Haedersdal, Catharina M. Lerche, Christian Janfelt
Summary: The aim of Quantitative mass spectrometry imaging (Q-MSI) is to provide distribution analysis and quantitation in one experiment. Mimetic tissue models based on spiked tissue homogenates are accurate methods for Q-MSI. The authors extended this model for skin studies, overcoming the high viscosity issue by adding water and handling the homogenate at an elevated temperature. They developed a method for quantitative imaging of bleomycin in skin using this model and included an internal standard to compensate for signal drift and skin inhomogeneities. The results of the model were comparable to a quantitative LC-MS analysis and it can replace or provide additional quantitative information to skin studies currently conducted by MSI alone.
Review
Spectroscopy
Colin T. McDowell, Xiaowei Lu, Anand S. Mehta, Peggi M. Angel, Richard R. Drake
Summary: This article introduces the development of glycan imaging mass spectrometry and discusses its applications in different sample types. Glycan imaging mass spectrometry analyses broaden our understanding of the biological and clinical relevance of N-glycosylation to human disease.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Biochemical Research Methods
Jonathan T. Specker, Boone M. Prentice
Summary: The diverse array of chemical compounds in tissue samples can result in many isobaric compounds in imaging mass spectrometry experiments. High-resolution accurate mass measurements can help resolve these compounds, but may not always be feasible. Alternatively, tandem mass spectrometry can be used to improve molecular specificity. Gas-phase ion/ion reactions can selectively react with desired analytes and differentiate isobaric compounds. This method has been used to differentiate isobaric lipids in the imaging mass spectrometry analysis of rat brain tissue.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Multidisciplinary
Qiuqin Zhou, Stefano Rizzo, Janina Oetjen, Annabelle Fueloep, Miriam Rittner, Hartmut Gillandt, Carsten Hopf
Summary: Insufficient vacuum stability of matrix chemicals is a major limitation in MALDI mass spectrometry imaging of large tissue sample cohorts. A photo-cleavable caged molecule, DMNB-2,5-DHAP, was designed and synthesized for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP exhibited vacuum stability in a high vacuum MALDI ion source and its uncaging process resulted in the generation of 2,5-DHAP with the assistance of the built-in laser in the MALDI ion source.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Plant Sciences
Wenxu Lu, Junhao Wang, Yanqiao Xie, Sijia Wu, Linnan Li, Hong Xu
Summary: The transcriptome, proteome, and metabolome are the material basis for the dynamic phenotypes in plants. Mass spectrometry imaging can provide spatial evidence of molecular components in pathways, which serves as direct physiological evidence for plant phenotypic changes.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Biochemical Research Methods
Tanja Bien, Elizabeth A. Hambleton, Klaus Dreisewerd, Jens Soltwisch
Summary: The Waminoa sp. acoel flatworms hosting Symbiodiniaceae and Amphidinium dinoflagellate algae provide an interesting model for symbiosis in marine environments. Through advanced imaging techniques, it was discovered that different sterol species play specific roles in the metabolism of Waminoa beyond simply serving as a source of energy, highlighting the value of this research for future spatially resolved analysis of sterols.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Wojciech Michno, Patrick M. Wehrli, Srinivas Koutarapu, Christian Marsching, Karolina Minta, Junyue Ge, Sven W. Meyer, Henrik Zetterberg, Kaj Blennow, Corinna Henkel, Janina Oetjen, Carsten Hopf, Jorg Hanrieder
Summary: This study utilized advanced chemical imaging tools to investigate the role of neuronal lipids in the pathophysiology of Alzheimer's disease, revealing the association between lipidomic microenvironment and structural polymorphism of Aβ plaques. The research also identified lipid patterns enriched and depleted at plaques, demonstrating the potential of multimodal imaging approaches to overcome limitations associated with conventional advanced MS imaging applications.
JOURNAL OF NEUROCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Johanna von Gerichten, Marwan H. Elnesr, Joe E. Prollins, Ishanki A. De Mel, Alan Flanagan, Jonathan D. Johnston, Barbara A. Fielding, Michael Short
Summary: This article discusses the use of the [C-13]octanoic acid breath test to label the solid phase of a meal and the factors that influence gastric emptying. It also highlights the potential benefits of a physiological based pharmacokinetic model approach in nutritional studies.
Article
Biochemistry & Molecular Biology
Matt Spick, Amy Campbell, Ivona Baricevic-Jones, Johanna von Gerichten, Holly-May Lewis, Cecile F. Frampas, Katie Longman, Alexander Stewart, Deborah Dunn-Walters, Debra J. Skene, Nophar Geifman, Anthony D. Whetton, Melanie J. Bailey
Summary: Treatments for COVID-19 infections have improved, with glucocorticoids playing a key role in reducing mortality rates. However, a study has found that glucocorticoids are not fully effective in addressing the diverse range of 'omics dysregulation caused by COVID-19 infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Holly-May Lewis, Priyanka Gupta, Kyle D. G. Saunders, Shazneil Briones, Johanna von Gerichten, Paul A. Townsend, Eirini Velliou, Dany J. V. Beste, Olivier Cexus, Roger Webb, Melanie J. Bailey
Summary: This work presents a new approach for measuring drug levels and lipid fingerprints in individual living mammalian cells. The method combines nanocapillary sampling with liquid chromatography to improve precision in drug analysis. By optimizing the transfer of analytes from the sampling capillary, the researchers achieved efficient measurement of drug analytes in 30 living cells. They also discovered cell-to-cell heterogeneity in drug molecule uptake and identified lipid features associated with bedaquiline uptake.
Article
Chemistry, Multidisciplinary
Janne J. Wiedmann, Yelda N. Demirdoegen, Stefan Schmidt, Mariia A. Kuzina, Yanchen Wu, Fei Wang, Britta Nestler, Carsten Hopf, Pavel A. Levkin
Summary: In the current drug discovery process, the synthesis of compound libraries is separated from biological screenings due to the challenge of high-throughput purification. This study demonstrates a miniaturized high-throughput liquid-liquid extraction method on a chip, with efficiency comparable to large-scale extraction. The new purification method adds an important tool to accelerate drug discovery by combining chemical combinatorial synthesis with biological screenings on a miniaturized microarray platform.
Article
Biochemistry & Molecular Biology
Hagen M. Gegner, Thomas Naake, Aurelien Dugourd, Torsten Mueller, Felix Czernilofsky, Georg Kliewer, Evelyn Jaeger, Barbara Helm, Nina Kunze-Rohrbach, Ursula Klingmueller, Carsten Hopf, Carsten Mueller-Tidow, Sascha Dietrich, Julio Saez-Rodriguez, Wolfgang Huber, Rudiger Hell, Gernot Poschet, Jeroen Krijgsveld
Summary: Metabolomic and proteomic analyses of human plasma and serum samples have the potential to advance our understanding of disease biology. However, pre-analytical factors such as temperature and time can impact the integrity of the samples and there is a need for standardized operating procedures to ensure reliable data.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Qiuqin Zhou, Stefano Rizzo, Janina Oetjen, Annabelle Fueloep, Miriam Rittner, Hartmut Gillandt, Carsten Hopf
Summary: Insufficient vacuum stability of matrix chemicals is a major limitation in MALDI mass spectrometry imaging of large tissue sample cohorts. A photo-cleavable caged molecule, DMNB-2,5-DHAP, was designed and synthesized for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP exhibited vacuum stability in a high vacuum MALDI ion source and its uncaging process resulted in the generation of 2,5-DHAP with the assistance of the built-in laser in the MALDI ion source.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Nicola A. Irvine, Annette L. West, Johanna Von Gerichten, Elizabeth A. Miles, Karen A. Lillycrop, Philip C. Calder, Barbara A. Fielding, Graham C. Burdge
Summary: This study compared the effect of incubation with 24:6 omega-3 on the fatty acid composition of two related cell types and found that cells can assimilate and metabolize exogenous 24:6 omega-3. The findings also suggest that consuming 24:6 omega-3 may provide similar health benefits as 20:5 omega-3 and 22:6 omega-3.
Article
Multidisciplinary Sciences
Denis Abu Sammour, James L. Cairns, Tobias Boskamp, Christian Marsching, Tobias Kessler, Carina Ramallo Guevara, Verena Panitz, Ahmed Sadik, Jonas Cordes, Stefan Schmidt, Shad A. Mohammed, Miriam F. Rittel, Mirco Friedrich, Michael Platten, Ivo Wolf, Andreas von Deimling, Christiane A. Opitz, Wolfgang Wick, Carsten Hopf
Summary: Mass spectrometry imaging utilizes traditional ion images for metabolite visualization and analysis, but lacks consideration for nonlinearities and statistical significance. The computational framework moleculaR aims to improve signal reliability by Gaussian-weighting ion intensities and introduces probabilistic molecular mapping for statistically significant spatial abundance analysis. It allows cross-tissue comparisons and spatial statistical significance evaluation, facilitating the investigation of ion milieus, lipid remodeling pathways, and complex scores within the same image.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Johanna von Gerichten, Annette L. West, Nicola A. Irvine, Elizabeth A. Miles, Philip C. Calder, Karen A. Lillycrop, Graham C. Burdge, Barbara A. Fielding
Summary: Immune function changes with age, and oxylipin synthesis might mediate these changes. This study investigated the impact of essential fatty acid (EFA) ratio on oxylipin secretion by T cells and whether this changes across life stages. The results showed that the EFA ratio influenced the extracellular oxylipin profile more than mitogen stimulation, and fetal T cells had higher oxylipin concentrations compared to adult and senior T cells. The capacity of T cells to synthesize oxylipins might contribute to their immunological phenotype.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Miriam F. Rittel, Stefan Schmidt, Cleo-Aron Weis, Emrullah Birgin, Bjoern van Marwick, Matthias Raedle, Steffen J. Diehl, Nuh N. Rahbari, Alexander Marx, Carsten Hopf
Summary: The current focus of cancer pathology diagnosis is on histopathological analysis using stained tissue and immunohistochemistry of marker proteins. However, spatial omics imaging is an emerging diagnostic technology for identifying and classifying different cancer types. To address the need for preserving tissue-specific metabolomic states, we developed a device and corresponding workflows for multimodal spatial omics analysis of fresh-frozen needle biopsies, allowing for a spatial comparison between spectral profiles and tissue histology without the need for an extra biopsy.
Article
Biology
Thomas Kupke, Rabea M. Goetz, Florian M. Richter, Rainer Beck, Fabio Lolicato, Walter Nickel, Carsten Hopf, Britta Bruegger
Summary: The article introduces a simple assay, iCLiPSpy, for studying intramembrane-cleaving proteases (I-CLiPs) in vivo. This method allows for easy detection of enzyme activity and investigation of residues important for substrate binding and activity. It has the potential to be used as a screening assay for I-CLiP inhibitors or activators.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Analytical
Kyle D. G. Saunders, Johanna von Gerichten, Holly-May Lewis, Priyanka Gupta, Matt Spick, Catia Costa, Eirini Velliou, Melanie J. Bailey
Summary: In this study, nanocapillary sampling combined with liquid chromatography-mass spectrometry was used for single-cell lipidomics. The method showed high sensitivity, allowing the tentative identification of around 260 lipids in a single cell. By analyzing the lipid profiles, chemotherapeutic drug-treated cancer cells were distinguished from control cells, and the relative abundance of LPC(0:0/16:0) was found to be significantly affected.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Anthony Onoja, Johanna von Gerichten, Holly-May Lewis, Melanie J. Bailey, Debra J. Skene, Nophar Geifman, Matt Spick
Summary: The global COVID-19 pandemic has led to rapid advancements in vaccine development, diagnostic testing, and treatment. This study evaluates the robustness of proposed biomarker panels in distinguishing COVID-19-positive and negative patients in a hospital setting. The best-performing panel consists of nine biomarkers and shows promising results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
