Article
Biochemistry & Molecular Biology
Maria G. Otero, Shaughn Bell, Alexander H. Laperle, George Lawless, Zachary Myers, Marian A. Castro, Jaquelyn M. Villalba, Clive N. Svendsen
Summary: Conventional tissue culture platforms limit the study of cell biology due to lack of maturity, while organ-chip microfluidic systems provide a more physiological environment for cell growth. In this study, organ-chip cultures showed a higher proportion and homogeneity of mature dopamine neurons compared to multi-well cultures. These organ-chips are ideal for studying the biology of mature dopamine neurons in health and neurological disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Minoru Hirano, Yike Huang, Daniel Vela Jarquin, Rosakaren Ludivina De la Garza Hernandez, Yasamin A. Jodat, Eder Luna Ceron, Luis Enrique Garcia-Rivera, Su Ryon Shin
Summary: This study introduces a biofabrication approach using 3D bioprinting and human pluripotent stem cell (hPSC) technologies to engineer sensible tissue constructs with complex microarchitecture wiring, demonstrating responsiveness to external stimuli with the potential for regenerating and enhancing sensory functions.
Article
Immunology
Evangelos Konstantinidis, Benjamin Portal, Tobias Mothes, Chiara Beretta, Maria Lindskog, Anna Erlandsson
Summary: This study reveals that astrocytes in Alzheimer's disease (AD) are not only associated with the pathology but also play a crucial role in maintaining brain homeostasis and synaptic function. The accumulation of aggregated amyloid-beta (A beta) in astrocytes affects their interaction with neurons, leading to synaptic dysfunction and neuronal apoptosis. These findings are important for understanding the involvement of astrocytes in AD-related synaptic dysfunction.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Cell Biology
Lisa M. Henkel, Svenja Kankowski, Thiemo M. Moellenkamp, Nadine J. Smandzich, Sigrid Schwarz, Alessio Di Fonzo, Gudrun Goehring, Guenter Hoeglinger, Florian Wegner
Summary: In this study, induced pluripotent stem cells (iPSCs) were generated and differentiated into medium spiny neurons (MSNs) in the striatum from patients with probable MSA-P and healthy controls. The MSNs derived from MSA-P patients showed significantly elevated release and neuronal distribution of alpha-synuclein, as well as hypoexcitability compared to healthy controls. These findings suggest that hypoexcitable neurons in the striatum of MSA-P patients contribute to the pathological burden of alpha-synuclein and facilitate disease progression.
Article
Cell & Tissue Engineering
Martin Barak, Veronika Fedorova, Veronika Pospisilova, Jan Raska, Simona Vochyanova, Jiri Sedmik, Hana Hribkova, Hana Klimova, Tereza Vanova, Dasa Bohaciakova
Summary: Induced pluripotent stem cells (iPSCs) have been widely used in the past two decades to study human neural development, disease modeling, and in vitro drug discovery. In the field of Alzheimer's disease (AD), iPSC-based models have been used to investigate molecular mechanisms and test potential drugs. This review summarizes the differentiation strategies of iPSCs into neuronal and glial cell types and cerebral organoids, and their applications in AD modeling and drug discovery.
STEM CELL REVIEWS AND REPORTS
(2022)
Article
Cell Biology
Emilia Solomon, Katie Davis-Anderson, Blake Hovde, Sofiya Micheva-Viteva, Jennifer Foster Harris, Scott Twary, Rashi Iyer
Summary: The study identified and characterized gene networks triggered by chemical reprogramming iPSC into motor neurons, providing insights into the differentiation process. The findings have implications for novel therapeutic approaches in regenerative medicine.
BMC MOLECULAR AND CELL BIOLOGY
(2021)
Article
Physiology
Merouann Kassa, Jonathan Bradley, Abdelali Jalil, Isabel Llano
Summary: Using optical activation, this study reveals that neuronal activity in the cerebellar cortex can be increased but also paused after the stimulation. The post-stimulation silencing is likely due to the activation of specific potassium channels.
JOURNAL OF GENERAL PHYSIOLOGY
(2022)
Article
Neurosciences
J. Alberto Ortega, Carissa L. Sirois, Fani Memi, Nicole Glidden, Nada Zecevic
Article
Genetics & Heredity
Nora Urraca, Brian Potter, Rachel Hundley, Eniko K. Pivnick, Kathryn McVicar, Ronald L. Thibert, Christopher Ledbetter, Reed Chamberlain, Leticia Miravalle, Carissa L. Sirois, Stormy Chamberlain, Lawrence T. Reiter
FRONTIERS IN GENETICS
(2016)
Article
Multidisciplinary Sciences
James J. Fink, Tiwanna M. Robinson, Noelle D. Germain, Carissa L. Sirois, Kaitlyn A. Bolduc, Amanda J. Ward, Frank Rigo, Stormy J. Chamberlain, Eric S. Levine
NATURE COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Kristen Martins-Taylor, Jack S. Hsiao, Pin-Fang Chen, Heather Glatt-Deeley, Adam J. De Smith, Alexandra I. F. Blakemore, Marc Lalande, Stormy J. Chamberlain
HUMAN MOLECULAR GENETICS
(2014)
Article
Multidisciplinary Sciences
Ian F. King, Chandri N. Yandava, Angela M. Mabb, Jack S. Hsiao, Hsien-Sung Huang, Brandon L. Pearson, J. Mauro Calabrese, Joshua Starmer, Joel S. Parker, Terry Magnuson, Stormy J. Chamberlain, Benjamin D. Philpot, Mark J. Zylka
Article
Multidisciplinary Sciences
Anna R. Moore, Wen-Liang Zhou, Carissa L. Sirois, Glenn S. Belinsky, Nada Zecevic, Srdjan D. Antic
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Cell & Tissue Engineering
Glenn S. Belinsky, Matthew T. Rich, Carissa L. Sirois, Shaina M. Short, Erika Pedrosa, Herbert M. Lachman, Srdjan D. Antic
STEM CELL RESEARCH
(2014)
Article
Cell & Tissue Engineering
Glenn S. Belinsky, Carissa L. Sirois, Matthew T. Rich, Shaina M. Short, Anna R. Moore, Sarah E. Gilbert, Srdjan D. Antic
STEM CELLS AND DEVELOPMENT
(2013)
Article
Biochemistry & Molecular Biology
Carissa L. Sirois, Judy E. Bloom, James J. Fink, Dea Gorka, Steffen Keller, Noelle D. Germain, Eric S. Levine, Stormy J. Chamberlain
HUMAN MOLECULAR GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Minjie Shen, Yu Guo, Qiping Dong, Yu Gao, Michael E. Stockton, Meng Li, Sudharsan Kannan, Tomer Korabelnikov, Keegan A. Schoeller, Carissa L. Sirois, Chen Zhou, Jonathan Le, Daifeng Wang, Qiang Chang, Qian-Quan Sun, Xinyu Zhao
Summary: Parvalbumin interneurons (PVIs) are influenced by the regulation of the FXR1 gene, impacting behaviors and oscillations related to schizophrenia. This reveals a potential mechanism of action for FXR1 in schizophrenia.
MOLECULAR PSYCHIATRY
(2021)
Article
Multidisciplinary Sciences
Yu Guo, Minjie Shen, Qiping Dong, Natasha M. Mendez-Albelo, Sabrina X. Huang, Carissa L. Sirois, Jonathan Le, Meng Li, Ezra D. Jarzembowski, Keegan A. Schoeller, Michael E. Stockton, Vanessa L. Horner, Andre M. M. Sousa, Yu Gao, Jon E. Levine, Daifeng Wang, Qiang Chang, Xinyu Zhao, Ian A. Glass, Dan Doherty
Summary: The study shows that the absence of FMRP leads to an increase in MAP1B, which inhibits neuronal development and social behavior. It also reduces autophagosome formation. Lowering MAP1B levels and activating autophagy can rescue the neuronal deficits in ASD and FXS.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Chenfeng He, Noah Cohen Kalafut, Soraya O. Sandoval, Ryan Risgaard, Carissa L. Sirois, Chen Yang, Saniya Khullar, Marin Suzuki, Xiang Huang, Qiang Chang, Xinyu Zhao, Andre M. M. Sousa, Daifeng Wang
Summary: Our machine-learning framework BOMA aligns developmental gene expression data between brains and organoids, revealing conserved and specific developmental trajectories. BOMA identifies organoid-preserved expression programs in certain brain regions and highly conserved gene expression around birth. Through analysis of scRNA-seq data, BOMA shows conserved and specific cell trajectories. Validation experiments confirm important expressed genes. BOMA is an open-source web tool for community use.
CELL REPORTS METHODS
(2023)
Article
Biochemistry & Molecular Biology
Fabian Offensperger, Franziska Mueller, Jasmin Jansen, Daniel Hammler, Kathrin H. Goetz, Andreas Marx, Carissa L. Sirois, Stormy J. Chamberlain, Florian Stengel, Martin Scheffner
CELL CHEMICAL BIOLOGY
(2020)