Article
Chemistry, Medicinal
Yong-Feng Wei, Xuan Wang, Wen-Jing Shi, Ru Chen, Liyao Zheng, Zi-Zhou Wang, Kun Chen, Liqian Gao
Summary: Hydrogen peroxide plays vital roles in living organisms, and there is a need to develop better probes for its detection. A novel fluorescent probe has been designed with high selectivity and sensitivity, suitable for detecting the presence of H2O2 in aqueous solutions and living cells.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Mingming Zhu, Ting Wang, Yutao Zhang, Jiahuan Nong, Zhiqian Guo
Summary: In this study, a novel fluorescent probe DFB-1 was synthesized which showed dual-channel fluorescence response, mitochondria-targeting ability, and high signal-to-background ratio. DFB-1 could track and differentiate the fluctuation of endogenous H2O2 in hepatocellular carcinoma and colorectal cancer cells. This probe provides new insights into the function of mitochondrial endogenous H2O2.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Jyun-Jia Ciou, Tzu-Chien Wu, Zhi-Jia Chen, Bill Cheng, Chian-Hui Lai
Summary: A fluorescent probe, HCyB, based on hemicyanine and arylboronate structures, was designed and successfully used to detect hydrogen peroxide (H2O2) with good linear relationship and selectivity. HCyB exhibited low toxicity, mitochondrial-targeting abilities, and could be used for monitoring H2O2 in different cell lines.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jiaxin Hong, Xiaogang Guan, Yao Chen, Xiaodong Tan, Shiya Zhang, Guoqiang Feng
Summary: Mitophagy is a crucial cellular process that plays vital roles in regulating cellular metabolism and mitochondrial quality control. Mitochondrial viscosity, closely associated with mitochondrial status, is a key microenvironmental index. To monitor mitophagy and mitochondrial viscosity changes, a probe called Mito-3 was developed. Mito-3, along with other probes, showed sensitivity to viscosity changes and fluorescence enhancement. These probes were successfully used to locate and visualize mitochondria, as well as monitor mitochondrial viscosity changes in cells. Additionally, Mito-3 was applied to visualize the mitophagy process induced by starvation and revealed an increase in mitochondrial viscosity during mitophagy.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Shuoshuo Wang, Jipeng Yao, Bei Wang, Xiang Liu
Summary: Developing an effective fluorescent probe for detecting hydrogen peroxide is urgently needed. Probe Z, constructed with naphthimide as the fluorophore and phenylboronic acid pinacol esters as the recognition group, shows higher sensitivity, lower detection limit, higher selectivity, and broad pH applicability. It has low cytotoxicity and can be used to detect exogenous hydrogen peroxide in HeLa cells, making it a potential tool for studying hydrogen peroxide in physiological activities.
Article
Optics
Xipeng Li, Yu Zhao, Judun Zheng, Tao Zhang
Summary: Hydrogen peroxide, as a typical reactive oxygen species, has attracted increasing interest in biochemical field. Researchers have developed a fluorescent probe BOD-H2O2 for highly sensitive and selective detection of H2O2, and it can visualize endogenous H2O2 in cells.
JOURNAL OF LUMINESCENCE
(2021)
Article
Chemistry, Applied
Jun Liu, Huizi Man, Chen Chang, Qingyue Liu, Quanda Yu, Shiyu Wang, Peng Yang, Yi Xiao, Mingzhe Liu
Summary: A new nanosensor called fluorescent probe-encapsulated reduction-sensitive smart nanohydrogel was developed in this study. The nanohydrogel was prepared using distillation-precipitation polymerization with N, N'-bis (acryloyl) cystinamide as the monomer and crosslinker. The hydrophobic peroxyresorufin-1 (PR1) probe for H2O2 was encapsulated into the nanohydrogel. The nanohydrogel showed high loading capacity for hydrophobic fluorescent probes and prevented their aggregation. The study demonstrated that the PR1-encapsulated nanohydrogel efficiently delivered the probes into living HeLa cells and enhanced the imaging of endogenous H2O2.
Article
Chemistry, Analytical
Ge Chunpo, Yang Yan, Tan Pengfei, Shi Hu, Jin Yibo, Shang Yuyang, Yang Yun, Ren Feng
Summary: This study developed a turn-on fluorescent probe, TMN-H2O2, with near-infrared emission for H2O2 detection in living systems, showing high selectivity, sensitivity, and fast response time. The successful application of TMN-H2O2 in vitro and in vivo suggests it is a reliable chemical tool for monitoring both endogenous and exogenous H2O2.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Xin Wang, Qi Ding, Ying Tian, Wei Wu, Feida Che, Ping Li, Wen Zhang, Wei Zhang, Bo Tang
Summary: This study developed two fluorescent probes to monitor H2O2 in mitochondria and lysosomes. The findings from fluorescence imaging demonstrated that mitochondrial H2O2 is involved in reduced GCase activity in lysosomes in depressed mice, providing a valuable tool for understanding the pathogenesis of depression.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Applied
Changquan Tang, Mingxue Wang, Changfeng Wu, Qingdong Zheng
Summary: A new fluorescence turn-on probe (MTNO) is designed and synthesized for monitoring mitochondrial nitric oxide (NO), with high sensitivity (detection limit of 14 nM), good mitochondrial localization, pH insensitivity, and high selectivity to NO. Two-photon microscopy imaging of exogenous and endogenous NO in living cells and tissue slices is achieved using MTNO.
Article
Chemistry, Applied
Yu Min Wang, Jiali Li, Jian Hua Chen, Bo Liu, Yanyan Zeng, Ja-Yun Lim, Ya Ting Liu, Jun Feng Zhang, Xiang Hua Wu, Wen Xiu Ren
Summary: A dual-responsive fluorescent probe FDOCl-N-Na was synthesized for detecting two reactive oxygen species (ROSs) H2O2 and HClO. FDOCl-N-Na exhibited distinct responses towards H2O2 and HClO, showing green and red fluorescence respectively. It could simultaneously monitor the cellular changes of H2O2 and HClO with high selectivity and sensitivity over a wide pH range. Cell imaging experiments demonstrated its ability to differentiate cancer cells from normal cells based on the abnormal expression of H2O2 and HClO. Moreover, FDOCl-N-Na was also employed for tracking the fluctuation of H2O2 and HClO in Caenorhabditis elegans.
Article
Chemistry, Analytical
Di-Hua Tian, Jun-Ru Liu, Si-Yuan Wang, Shuai Yan, Zuo-Hu Chai, Fang Dai, Shengxiang Zhang, Bo Zhou
Summary: This study developed a new boronate-based fluorescent probe for selective imaging of H2O2 over ONOO-. The probe utilizes a specific reaction to release a fluorescent substance, resulting in a ratiometric fluorescence response. By using this probe, the increased levels of H2O2 during cellular processes and in live animals were successfully visualized.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Kangkang Wang, Tingting Yao, Jiayu Xue, Yanqiu Guo, Xiaowei Xu
Summary: Hydrogen peroxide (H2O2), an important reactive oxygen species (ROS), is closely related to various pathological and physiological processes in living organisms. Excessive H2O2 can lead to diseases such as cancer, diabetes, and cardiovascular diseases, necessitating the detection of H2O2 in living cells. This study designed a new fluorescent probe that effectively detects H2O2 with high selectivity and measures cellular ROS levels, providing a potential monitoring tool for various diseases caused by H2O2 excess.
Article
Chemistry, Analytical
Zhongjiao Feng, Jiasheng Wu, Weimin Liu, Haohui Ren, Xiuli Zheng, Wenjun Zhang, Chun-Sing Lee, Pengfei Wang
Summary: A Golgi-targeting two-photon fluorescent probe was designed to detect hydrogen peroxide with high sensitivity and selectivity. It can be used for visualization of endogenous/exogenous H2O2 in vitro and in vivo.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Rongrong Zhou, Qiyao Peng, Dan Wan, Chao Yu, Yuan Zhang, Yi Hou, Quan Luo, Xiong Li, Shuihan Zhang, Lin Xie, Pinghua Ou, Yongbo Peng
Summary: A new lysosome-targeting fluorescent probe NPT-H2O2 was developed for ratiometric detection and imaging of H2O2, especially in lysosomes and inflammation models. The probe exhibited rapid response, high selectivity and sensitivity towards H2O2, along with low cytotoxicity. The results suggest that this probe can serve as a powerful tool to investigate the physiological and pathological relationship between inflammation and lysosomal H2O2.
Article
Chemistry, Analytical
Xueli Wang, Chengke Wang, Hei-Nga Chan, Iyaswamy Ashok, Senthil Kumar Krishnamoorthi, Min Li, Hung-Wing Li, Man Shing Wong
Summary: Novel near infrared emissive fluorophores exhibit strong selectivity towards Aβ oligomers and monomers, good biocompatibility and stability, and significant inhibitory effects on Aβ aggregation.
Article
Chemistry, Analytical
Mingtai Sun, Tian Wang, Xin Yang, Huan Yu, Suhua Wang, Dejian Huang
Summary: A new fluorescent probe BV-1 based on BODIPY group was synthesized and demonstrated to detect viscosity in living cells with high selectivity and sensitivity. The probe showed significant enhancement in fluorescence with redshift in emission wavelength under high viscosity conditions, providing efficient ways for the development of viscosity probes and monitoring of intracellular viscosity properties and functions.
Article
Chemistry, Analytical
Huanxin Xue, Jiaye Lu, Hongwei Yan, Ju Huang, Hai-Bin Luo, Man Shing Wong, Yuqi Gao, Xiaolei Zhang, Lei Guo
Summary: In this study, a novel organic fluorescent probe was developed for rapid detection of lung cancer cells and specific targeting of the nucleolus in the cell. By interfering with rRNA synthesis in the nucleolus, the probe induced cell apoptosis, showing promising anti-cancer effects.
Article
Chemistry, Multidisciplinary
Song-Lin Zhang, Long Yu, Peng-Chen Su, Hong-Wei Ge, Ming-Tai Sun, Su-Hua Wang
Summary: The excessive use of antibiotics in agriculture leads to contamination in food and water. This paper synthesized a zinc(II)-trimesic acid metal-organic framework and developed a test paper for the visual detection of chlortetracycline. The test paper showed potential application in water quality monitoring.
Article
Biochemistry & Molecular Biology
Ashok Iyaswamy, Xueli Wang, Senthilkumar Krishnamoorthi, Venkatapathy Kaliamoorthy, Sravan G. Sreenivasmurthy, Siva Sundara Kumar Durairajan, Ju-Xian Song, Benjamin Chun-kit Tong, Zhou Zhu, Cheng-Fu Su, Jia Liu, King-Ho Cheung, Jia-Hong Lu, Jie-Qiong Tan, Hung Wing Li, Man Shing Wong, Min Li
Summary: The accumulation of phosphorylated Tau aggregates and A beta oligomers are crucial factors in the development of Alzheimer's disease. A study found that the fluorescent probe F-SLOH effectively inhibited A beta aggregation and reduced various neuropathological changes in AD mouse models by promoting autophagy lysosomal degradation pathway.
Article
Chemistry, Analytical
Xin Yang, Justin Lee Kee Leong, Mingtai Sun, Linzhi Jing, Yuannian Zhang, Tian Wang, Suhua Wang, Dejian Huang
Summary: We designed a smartphone-based optical fiber sensor for rapid and portable detection of ethylene in commercial fruit ripening storage rooms. The sensor showed a wide linearity range with a low detection limit, and was not significantly affected by common gases in the warehouse. The device is compact and portable, with an inbuilt power supply and replaceable sensor tip.
Article
Nanoscience & Nanotechnology
Xueli Wang, Ashok Iyaswamy, Di Xu, Senthilkumar Krishnamoorthi, Sravan Gopalkrishnashetty Sreenivasmurthy, Yuncong Yang, Yinhui Li, Chen Chen, Min Li, Hung-Wing Li, Man Shing Wong
Summary: This study reports the development of the first A beta-targeted ratiometric H2O2-responsive fluorescent probe for real-time detection and monitoring of A beta-induced H2O2 levels. This probe can be used as a powerful tool for diagnosing high-risk subjects and diseased brains of AD, as well as studying the role of ROS in AD pathology.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Xuewei Liu, Anrui Zhang, Ran Ma, Bo Wu, Tao Wen, Yuejie Ai, Mingtai Sun, Jie Jin, Suhua Wang, Xiangke Wang
Summary: In this study, a microwave irradiation method was developed to synthesize copper phthalocyanine-based covalent organic frameworks (CuxPc-COFs) with excellent iodine enrichment performance. The charge transfer arising from nitrogen-rich phthalocyanine structures and electron-rich pi-conjugated systems with iodine molecules contributes to the impressive adsorption performance. The electrostatic interaction between Cu(II) on chelate centers and polyiodide anions also plays a crucial role in trapping radioactive iodine. This research provides a facile and intelligent approach for the remediation of toxic radioactive iodine using metal-based COFs.
CHINESE CHEMICAL LETTERS
(2022)
Article
Agriculture, Multidisciplinary
Xin Yang, Tian Wang, Petar Zuvela, Mingtai Sun, Chunyuhang Xu, Hongling Zheng, Xiang Wang, Linzhi Jing, Ke Du, Suhua Wang, Ming Wah Wong, Dejian Huang
Summary: Flavonoids, as effective hypochlorite scavengers, exhibit a clear relationship between their structure and activity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Surgery
Hongyu Lyu, Yongqin Ye, Vincent Chi Hang Lui, Weifang Wu, Patrick Ho Yu Chung, Kenneth Kak Yuen Wong, Hung-Wing Li, Man Shing Wong, Paul Kwong Hang Tam, Bin Wang
Summary: Elevated plasma levels of A beta 42/A beta 40 were found in patients with biliary atresia (BA), showing correlations with liver function parameters and good predictive power for cholestasis. Combining A beta 42/A beta 40 with other hepatic function parameters significantly improved the diagnostic ability for BA, making it a potentially useful screening tool for jaundiced neonates.
FRONTIERS IN SURGERY
(2022)
Review
Biochemistry & Molecular Biology
Wenzhi Xie, Jinyu Jiang, Dunji Shu, Yanjun Zhang, Sheng Yang, Kai Zhang
Summary: This article summarizes recent advances in biothiol-responsive fluorescent probes, with a focus on rational probe design, including reaction mechanism, discriminating detection, reversible detection, and specific detection. Furthermore, the challenges and prospects of fluorescence probes for biothiols are also outlined.
Article
Chemistry, Analytical
Qiang Zhang, Lingxiao Wang, Pengchen Su, Long Yu, Ranhao Yin, Yiming Bu, Xiangyang Hao, Mingtai Sun, Suhua Wang
Summary: A method based on ratiometric fluorescence and colorimetric method using nitrogen-rich carbon dots as fluorophore was developed for selective and sensitive determination of ceftriaxone sodium (CTR) trace residues. The functional surfaces of indole-derived carbon dots (I-CDs) can selectively bind to CTR via electrostatic forces, resulting in a distinct variety in fluorescence and absorption wavelength and intensity. The detection limits (LODs) were estimated to be 19.7 nM and 78.0 nM for the ratiometric fluorescence method and colorimetric method, respectively. The nanosensor combined with a smartphone's color recognizer allowed for in situ visual quantitative determination of CTR.
Review
Chemistry, Analytical
Bo Liu, Honghui Yin, Yaxiong Li, Guojiang Mao, Sheng Yang, Kai Zhang
Summary: Fatty liver diseases, including benign fatty liver, can progress to cirrhosis and hepatocellular cancer without timely treatment. Liver biopsy, although a gold standard, is not recommended due to its invasiveness and complexity. Therefore, there is an urgent need for non-invasive and accurate methods for diagnosing fatty liver diseases. Fluorescence imaging technology has emerged as a robust method for fatty liver visualization, with the development of various fluorescent probes. This review summarizes the small molecular fluorescent probes used in fatty liver, focusing on the last four years, and highlights the current opportunities and challenges in the field.
Article
Chemistry, Inorganic & Nuclear
Hongxia Chen, Chao Yuan, Junxiang Peng, Mingtai Sun, Shaoquan Liu, Dejian Huang, Suhua Wang
Summary: This study reports a novel europium-doped zeolitic imidazolate framework (ZIF-Eu) nanocomposite as a ratiometric fluorescent probe for the detection of doxycycline. The probe exhibits a good linear range and low detection limit, and can differentiate from other tetracycline antibiotics. Furthermore, the direct detection of doxycycline in soil samples and the reusability of the ZIF-Eu nanocomposite in environmental remediation application were also demonstrated.
DALTON TRANSACTIONS
(2022)
Article
Chemistry, Multidisciplinary
Jiaping Zhu, Chaojian Yao, Ayan Maity, Jielai Xu, Tong Zhan, Weibing Liu, Mingtai Sun, Suhua Wang, Vivek Polshettiwar, Hua Tan
Summary: Nitrogen-doped carbon spheres with wrinkled cages (NCSWCs) were used for the first time as metal-free catalysts, exhibiting high catalytic activity and selectivity in the oxidation of 5-hydroxy-methylfurfural (HMF) to 5-formyl-2-furancarboxylic acid (FFCA) under base-free conditions using tert-butyl hydroperoxide (TBHP) as the oxidant. Mechanistic studies revealed that the reaction was catalyzed by the synergy between NCSWCs and TBHP, with hydroperoxyl radicals from TBHP adsorbed on the carbon atoms adjacent to the graphitic N atoms identified as the active sites.
CHEMICAL COMMUNICATIONS
(2021)
