Review
Food Science & Technology
Maria Micaela Ureta, Goncalo Nuno Martins, Onofre Figueira, Pedro Filipe Pires, Paula Cristina Castilho, Andrea Gomez-Zavaglia
Summary: This review explores principal immobilization strategies aimed at achieving optimal industrial processes, focusing specifically on beta-galactosidases and fructosyltransferases. The main methods for immobilizing these enzymes include adsorption, entrapment, covalent coupling, and cross-linking or aggregation. It is important for the support material to be cost-effective, ensure enzyme reusability and easy recovery, and enhance stability.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2021)
Article
Chemistry, Multidisciplinary
Hongmei Wang, Shengbao Duan, Yezhou Chen, Huan Liu, Jingjing Tian, Feiran Wu, Ziqian Du, Longhai Tang, Yong Li, Shaohua Ding
Summary: This study investigates the potential of using a natural silk cocoon membrane as a substrate for immunosensing assays. The membrane exhibits excellent bioactivity and stability, making it a versatile and thermally stable platform for immunoassays.
Review
Chemistry, Multidisciplinary
Zaixing Li, Chao Li, Qinqin Zhang, Chen Hong, Wenjing Zhang, Bingbing Xu, Xue Qin, Guixia Li, Zhifang Ning
Summary: The practical application of enzymes as biocatalysts faces challenges such as inactivation and loss. Immobilized enzyme technology, especially using magnetic nanocomposites as carriers, shows promise in improving enzyme performance.
Article
Agriculture, Dairy & Animal Science
D. A. Hutchins, J. Noh, J. D. Kenealey
Summary: This study investigated the effects of various immobilized enzyme support materials, highlighting the superior performance of NFM with its larger surface area and higher enzyme activity. NFM demonstrated better stability of enzymes at different storage temperatures compared to other materials.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Selen Kizildag, Ceyhun Isik, Mustafa Teke
Summary: In this study, beta-galactosidase was successfully immobilized on eggshell membrane (ESM) using adsorption and cross-linking method. The interaction between enzyme and ESM was analyzed using ATR-FTIR and SEM. The immobilized enzyme showed improved stability and could retain more than 50% of its activity after multiple reuses. The highest lactose removal efficiency of 55.8% was achieved by using beta-galactosidase immobilized ESM under optimized conditions.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Lilian Mayumi Kuribayashi, Victoria Pires do Rio Ribeiro, Ricardo Correa de Santana, Eloizio Julio Ribeiro, Milla Gabriela dos Santos, Larissa Nayhara Soares Santana Falleiros, Carla Zanella Guidini
Summary: The study investigated the immobilization of beta-galactosidase from Bacillus licheniformis in ion exchange resin, identifying optimal conditions for enzyme activity and stability. By immobilizing the enzyme under specific conditions, it showed potential for increasing reusability and reducing operational costs. Further experiments with buffer pH and crosslinking with glutaraldehyde demonstrated significant impact on the stability and activity of the immobilized enzyme.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eduardo Rosado, Paloma Delgado-Fernandez, Blanca de las Rivas, Rosario Munoz, Francisco Javier Moreno, Nieves Corzo, Cesar Mateo
Summary: This study presents the synthesis of beta-galactosyl xylitol derivatives using immobilized LacA beta-galactosidase from Lactobacillus plantarum WCFS1. The immobilized enzyme exhibited high activity and stability, allowing for the synthesis of a large amount of beta-galactosyl xylitol derivatives with improved efficiency. It also showed lower in vitro intestinal digestibility compared to lactose.
Article
Agricultural Engineering
Shohreh Ariaeenejad, Elaheh Motamedi, Ghasem Hosseini Salekdeh
Summary: A novel immobilized enzyme on modified magnetic graphene oxide was used for efficient removal of dyes from water, showing increased stability and reusability compared to free enzymes.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Physical
Lucas A. de Freitas, Marylane de Sousa, Laiza B. Ribeiro, Italo W. L. de Franca, Luciana R. B. Goncalves
Summary: This study proposes cross-linked magnetic cross-linked enzyme aggregates (mCLEAs) for the preparation of heterogeneous biocatalysts of beta-galactosidase, and investigates the concentration of cross-linking agents glutaraldehyde and dextran-aldehyde. The use of glutaraldehyde results in increased recovered activity, while the addition of a competitive inhibitor or polymer coating in the preparation process does not enhance the activity. Biocatalysts prepared with dextran-aldehyde exhibit higher recovered activity and thermal stability, making them suitable for industrial use.
Article
Biochemistry & Molecular Biology
Pieter De Wever, Charlotte De Schepper, Claude Poleunis, Arnaud Delcorte, Christophe M. Courtin, Pedro Fardim
Summary: This study investigates the impact of different spacer arms on enzymatic activity and oligosaccharide yield of xylanase immobilized on cellulose beads. By surface functionalization of the beads with endo-1,4-beta-xylanase from Thermotoga maritima, xylooligosaccharides with degrees of polymerization (DPs) in the range of 4-6 were successfully produced. The findings highlight the importance of topochemical engineering and provide insights into the enzymatic production of longer xylooligosaccharides.
Review
Chemistry, Multidisciplinary
Rahul Vikram Singh, Krishika Sambyal
Summary: This review provides systematic information about the recent advancements in the production of beta-galactosidase and its industrial applications, primarily focusing on the application of recombinant strains and immobilization strategies. These developments have made beta-galactosidase an important enzyme in the industry.
Review
Polymer Science
Mohammad Iman Tayouri, Sara Estaji, Seyed Rasoul Mousavi, Samaneh Salkhi Khasraghi, Reza Jahanmardi, Sasan Nouranian, Mohammad Arjmand, Hossein Ali Khonakdar
Summary: This review paper provides an overview of the current research on the stabilization and degradation of GO and rGO-filled polymer nanocomposites. The structure, synthesis, properties, and applications of GO and rGO nanoparticles are discussed. The surface modification of graphene-based nanomaterials to improve their dispersion in polymers is also reviewed. The mechanisms associated with the thermal, photolytic, hydrolytic, and enzymatic degradation of the nanocomposites are summarized.
POLYMER DEGRADATION AND STABILITY
(2022)
Article
Microbiology
Maria Florencia Eberhardt, Jose Matias Irazoqui, Ariel Fernando Amadio
Summary: Stabilization ponds are commonly used for treating wastewater from dairy industries, where large amounts of cheese whey are disposed causing environmental issues. Through whole-genome shotgun sequencing of the metagenome from these ponds, novel genes encoding beta-galactosidases were identified. These enzymes offer alternative options for producing value-added products from dairy industry by-products.
Article
Biochemistry & Molecular Biology
Marcela Serey, Carlos Vera, Cecilia Guerrero, Andres Illanes
Summary: Aspergillus oryzae beta-galactosidase was immobilized in in-house quaternary ammonium agarose (QAA) for the first time to synthesis lactulose, resulting in a biocatalyst with high specific activity. The immobilized enzyme showed higher yield and specific productivity in batch reactions, and the support could be reused without compromising yield, productivity, and selectivity, reducing economic impact.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Anamaria Todea, Ioana Cristina Benea, Ioan Bitcan, Francisc Peter, Szilvia Klebert, Tivadar Feczko, Zoltan Karoly, Emese Biro
Summary: In this study, tailor-made immobilization of two beta-galactosidases and a glucose oxidase on specific supports enabled a one-pot cascade reaction converting lactose to gluconic acid and galacto-oligosaccharides. The immobilized biocatalysts showed enhanced storage, pH, and thermal stability, as well as better operational stability compared to the soluble enzymes.
Article
Biophysics
Irfanah Siddiqui, Qayyum Husain
COLLOIDS AND SURFACES B-BIOINTERFACES
(2019)
Article
Environmental Sciences
Nida Khan, Qayyum Husain
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2019)
Article
Materials Science, Biomaterials
Shamoon Asmat, Qayyum Husain
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2019)
Article
Chemistry, Applied
Maryam Khan, Qayyum Husain
Article
Biotechnology & Applied Microbiology
Maryam Khan, Qayyum Husain
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Shamoon Asmat, Abdul Hakeem Anwer, Qayyum Husain
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Nanoscience & Nanotechnology
Faizan Ahmed, Qayyum Husain, Mohd Owais Ansari, G. G. H. A. Shadab
APPLIED NANOSCIENCE
(2020)
Article
Biochemical Research Methods
Mohd Faisal Asar, Nafees Ahmad, Qayyum Husain
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Misha Ali, Hassan Mubarak Ishqi, Qayyum Husain
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biochemistry & Molecular Biology
Faizan Ahmed, Syed Mohammad Faisal, Anees Ahmed, Qayyum Husain
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2020)
Article
Materials Science, Biomaterials
Shamoon Asmat, Qayyum Husain, Mohd Shoeb, Mohammad Mobin
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Nida Khan, Qayyum Husain, Navira Qayyum
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biochemistry & Molecular Biology
Irfanah Siddiqui, Qayyum Husain, Ameer Azam
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biochemistry & Molecular Biology
Azra Shafi, Faizan Ahmed, Qayyum Husain
Summary: β-Galactosidase was immobilized on modified nanosilver reduced graphene oxide (Ag@rGO) nanocomposite prepared by in vitro synthesis using same enzyme. The immobilized enzyme showed enhanced activity with an effectiveness factor (eta value) of 0.968, and retained 85% activity after repeated use. The enzyme displayed high activity even in the presence of inhibitory sugars, and effectively hydrolyzed lactose from whey. Additionally, the cytotoxicity and genotoxicity of Ag@rGO nanocomposite was assessed on human blood lymphocytes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Endocrinology & Metabolism
Hira Ateeq, Afaf Zia, Qayyum Husain, Mohd Sajid Khan, Mohd Ahmad
Summary: Diabetes mellitus and periodontitis are inflammatory diseases that affect bone homeostasis, with diabetic complications aggravating in subjects with periodontitis. Inflammatory cytokines and immune responses, particularly the RANKL/OPG pathway, play a crucial role in the interaction between diabetic inflammation and periodontal disease, influencing bone resorption. Research focusing on regulating cytokine expression may help in controlling diabetes, periodontitis, and bone health.
JOURNAL OF DIABETES AND METABOLIC DISORDERS
(2022)