Article
Agricultural Engineering
Niling He, Chun Fang, Zhongyang Qiu, Jie Bao
Summary: In this study, by overexpressing Na+/H+ antiporters in Pediococcus acidilactici, the transmembrane transportation of Na+ was enhanced, leading to significantly improved sodium lactate production. Additionally, transcription analysis revealed that the overexpression of Na+/H+ antiporter upregulated the transcription of sugar phosphorylation genes under high Na+ stress in P. acidilactici.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anshula Sharma, Masafumi Noda, Masanori Sugiyama, Ajaz Ahmad, Baljinder Kaur
Summary: This study developed functional drinks through LAB fermentation of buttermilk and soymilk, leading to increased levels of antioxidants, phenolics, flavonoids, and L-alanine, as well as decreased levels of undesirable metabolites causing bitterness, rancidity, and unpleasant odor. The use of P. acidilactici BD16 (alaD(+)) as a starter culture candidate for functional buttermilk and soymilk production was highlighted for the first time.
Article
Agriculture, Multidisciplinary
Ali Muhammed Moula Ali, Maria Gullo, Amit Kumar Rai, Sri Charan Bindu Bavisetty
Summary: Lactic acid fermentation significantly increases the antioxidant activity of chicken gizzards while retaining important bio-iron and nutrients. Fermented chicken gizzards have the potential to be used as functional food or feed additives.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Food Science & Technology
Wenhui Fu, Chenglong Liu, Xiao Meng, Sha Tao, Wentong Xue
Summary: This study found that co-culturing yeast can accelerate protein degradation and significantly reduce allergen content in dough. RNA sequencing analysis showed that the presence of yeast can promote protein metabolism in Pediococcus acidilactici XZ31 for a certain period of time. These results suggest the potential use of multi-strain leavening agents for producing hypoallergenic wheat products.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Wenhui Fu, Chen Chen, Chenglong Liu, Sha Tao, Wentong Xue
Summary: This study investigated the effects of fermentation on the digestibility and allergenicity of wheat proteins. Co-culture fermentation with Pediococcus acidilactici XZ31 and yeast improved the digestibility of wheat proteins. Fermentation led to a decrease in albumin/globulin antigenicity and an increase in gluten R5 reactivity, with the most significant changes in the co-culture group. Pretreatment with P. acidilactici XZ31 reduced allergens and attenuated gluten-induced permeability increase in intestinal cells.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Biotechnology & Applied Microbiology
Anshula Sharma, Masafumi Noda, Masanori Sugiyama, Baljinder Kaur, Ajaz Ahmad
Summary: This study increased the production of L-alanine in recombinant strains through optimized fermentation processes, resulting in high-purity L-alanine production. The findings have great commercial potential and could contribute to the global expansion of the microbial L-alanine market.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Joana Campos, Jie Bao, Gunnar Liden
Summary: This study demonstrates that two modified strains efficiently utilize high mannose content in softwood hydrolysate to produce high-purity L and D-lactic acid. Despite lower mannose utilization rates compared to glucose, this research provides important insights for future production of chiral lactic acid from lignocellulosic feedstocks.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Su Xu, Xinyu Song, Jirong Liu, Wenjuan Zhang, Xiaochen Yu, Dianyu Yu, Jianjun Cheng
Summary: A strain of lactic acid bacteria, Pediococcus acidilactici, was isolated and combined with Bacillus subtilis and Saccharomyces cerevisiae to ferment raw corn-soybean meal. Through optimization, the fermented feed showed increased protein content and total amino acids, which is beneficial for pig growth.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Yixin Zhang, Musen Wang, Samaila Usman, Fuhou Li, Jie Bai, Jiayao Zhang, Xusheng Guo
Summary: Pediococcus acidilactici and an exogenous fibrolytic enzyme were used to improve the fermentation quality and lignocellulose conversion of Caragana korshinskii silage. The addition of P. acidilactici and Acremonium cellulase (PA + AC) resulted in the highest lactic and acetic acids, lowest non-protein nitrogen and ammonia nitrogen levels. The bacterial community in all silages was dominated by P. acidilactici, and the addition of P. acidilactici led to a simpler network of bacterial interaction and lower bacterial diversity. PA treatment exhibited lower structural carbohydrates degradation but performed better in lignocellulose conversion during enzymatic saccharification. These findings suggest that pretreating C. korshinskii with PA can improve its silage quality and make it a potential feedstock for bio-product and biofuel production.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Zhongyang Qiu, Xushen Han, Jianlong He, Yanan Jiang, Guangli Wang, Zejia Wang, Xiaoyan Liu, Jun Xia, Ning Xu, Aiyong He, Hanqi Gu, Jiaxing Xu
Summary: Inhibitor tolerance remains a challenge for lactic acid bacteria in lignocellulose biorefinery. This study obtained an engineered strain with high tolerance through long-term adaptive evolution and successfully achieved simultaneous saccharification and fermentation in one step.
BIORESOURCE TECHNOLOGY
(2022)
Article
Food Science & Technology
Anshula Sharma, Masafumi Noda, Masanori Sugiyama, Baljinder Kaur, Ajaz Ahmad
Summary: Metabolic engineering was utilized to improve the efficiency of microbial strains for high-level fermentative production of L-alanine. A synthetic alaD gene was successfully cloned and expressed in a recombinant P. acidilactici BD16 strain, resulting in a significant increase in L-alanine production. The developed bioprocess showed promise as a potential alternative to chemical-based commercial synthesis of L-alanine for industrial applications.
Article
Microbiology
Young-Ran Song, Chan-Mi Lee, Seon-Hye Lee, Sang-Ho Baik
Summary: PA-M76 demonstrated strong probiotic properties with high tolerance in GIT environments, broad antimicrobial activity, and effective immune response elicitation. Lactic acid fermentation of BRE by PA-M76 resulted in increased antioxidant activity, while not significantly affecting total anthocyanin and flavonoid content.
Article
Microbiology
Qiang Zhang, Cuimei Zhao, Xiaobin Wang, Xiaowei Li, Yu Zheng, Jia Song, Menglei Xia, Rongzhan Zhang, Min Wang
Summary: Bioaugmentation technology can improve the solid-state fermentation rate and utilization of raw materials for traditional vinegar production. By introducing Pediococcus acidilactici AAF1-5, the fermentation process was accelerated and the quality of vinegar was enhanced through increased levels of key microorganisms.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zhao Yan, Mingxing Chen, Jia Jia, Jie Bao
Summary: This study aimed to enhance the acid tolerance of an engineered Pediococcus acidilactici strain using metabolic modification and adaptive evolution approaches. While overexpression of certain genes did not show significant improvement, low pH adaptive evolution resulted in clear enhancement. However, further decrease in pH led to a dramatic reduction in L-lactic acid generation.
FERMENTATION-BASEL
(2022)
Article
Microbiology
Maria Carolina Wanderley Porto, Pamela Oliveira de Souza de Azevedo, Felipe Rebello Lourenco, Attilio Converti, Michele Vitolo, Ricardo Pinheiro de Souza Oliveira
Summary: This study investigated the effect of polydextrose on cell growth, lactic acid and bacteriocin-like inhibitory substance (BLIS) production in Pediococcus pentosaceus. The results showed that optimal conditions for maximum cell concentration, productivity, specific growth rate, yield of biomass, substrate consumption rate, volumetric lactic acid productivity, and antibacterial activity varied depending on pH, temperature, polydextrose concentration, and rotational speed.
Review
Food Science & Technology
Yee-Ying Lee, Teck-Kim Tang, Eng-Seng Chan, Eng-Tong Phuah, Oi-Ming Lai, Chin-Ping Tan, Yong Wang, Nur Azwani Ab Karim, Noorlida Habi Mat Dian, Joo Shun Tan
Summary: Structured lipids are a modified form of lipid aimed at improving the nutritional and functional properties of conventional fats and oils. Examples of this new generation of healthful lipids, such as MCT and MLCT, are mainly composed of MCFA and have shown multiple physiological and functional properties.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Engineering, Chemical
Roslina Jawan, Sahar Abbasiliasi, Joo Shun Tan, Mohd Rizal Kapri, Shuhaimi Mustafa, Murni Halim, Arbakariya B. Ariff
Summary: The study evaluated the influence of different types and concentrations of lyoprotectants, storage temperature, and duration on the cell viability and antibacterial activity of Lactococcus lactis Gh1. It was found that the type and concentration of lyoprotectants had minimal impact on cell viability and antibacterial activity, but significant differences were observed between concentrations during storage.
Article
Biochemical Research Methods
Hock Wei Tang, Sahar Abbasiliasi, Zhang Jin Ng, Yee-Ying Lee, Teck-Kim Tang, Joo Shun Tan
Summary: The study successfully produced Enterococcus faecium using optimization of fermentation medium and fed-batch cultivation strategy with sugarcane molasses as a carbon source. The results showed high cell viability and dry cell weight, demonstrating the industrial potential of molasses-based medium for probiotics production.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Marjan Sadat Hosseini, Morteza Ebrahimi, Javier Abadia, Saeid Kadkhodaei, Rasoul Amirian
Summary: Salinity affects the growth and biochemical characteristics of Glycyrrhiza, with decreases in biomass and leaf water content, and increases in antioxidant enzyme activities and osmolyte levels. Different genotypes show varying responses, with some experiencing decreases in glycyrrhizin concentration and content, while one genotype ('Kerman') shows moderate effects on glycyrrhizin content and gene expression.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agronomy
Daryush Talei, Mojtaba Khayam Nekouei, Saeid Kadkhodaei
Summary: This study investigated the effects of different concentrations of AgNPs on Trigonella foenum-graecum. The results showed that applying 10 mg/L of AgNPs resulted in the highest biomass and chlorophyll content, while applying 20 mg/L of AgNPs resulted in the highest protein content. High concentrations of AgNPs led to reduced growth and yield. Therefore, using a low concentration of AgNPs can improve the growth and yield of Trigonella foenum-graecum.
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS
(2023)
Review
Food Science & Technology
X. Y. Lee, J. S. Tan, L. H. Cheng
Summary: This mini review discusses the biosynthetic pathways of GABA in plants and its presence in foods. Compared to animal-based foods, plant-based foods generally have lower levels of GABA, although germination and fermentation can increase GABA content. However, there is limited research on the stability of GABA during processing and storage, its bioavailability, and clinical implications.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Microbiology
Abid Sarwar, Sam Al-Dalali, Tariq Aziz, Zhennai Yang, Jalal Ud Din, Ayaz Ali Khan, Zubaida Daudzai, Quratulain Syed, Rubina Nelofer, Nazif Ullah Qazi, Zhang Jian, Anas S. Dablool
Summary: The addition of S. boulardii and inulin in fermented yogurt resulted in higher antioxidant and volatile compounds, making it a favorable choice for consumers.
Article
Biochemical Research Methods
Zhang Jin Ng, Sahar Abbasiliasi, Tam Yew Joon, Hui Suan Ng, Pongsathon Phapugrangkul, Joo Shun Tan
Summary: Porous glass beads grafted with polyethylene glycol (PEG) were used as an adsorbent to purify lipase from Burkholderia metallica in column chromatography. The effects of various purification parameters such as salt stability, types and concentrations of PEG and salt, pH of the binding solution, and flow rate were investigated. It was found that using 20% (w/w) PEG 6000 g/mol impregnated glass beads with a binding solution of 5% sodium citrate at pH 7.7, a flow rate of 1.0 mL/min and extraction time of 10 min resulted in the highest purification factor and recovery yield at 3.67 and 88%, respectively.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Seyed Morteza Zahedi, Marjan Sadat Hosseini, Narjes Fahadi Hoveizeh, Saeid Kadkhodaei, Marek Vaculik
Summary: Improving adaptation and choosing tolerant cultivars are crucial to mitigate water limitations in plants, especially strawberries. In this study, two strawberry cultivars, Camarosa and Gaviota, were compared under different drought conditions. Drought stress resulted in reduced chlorophyll content, increased oxidative markers, and other physiological changes. Gaviota exhibited higher proline production and electrolyte leakage, while Camarosa had more soluble carbohydrates and antioxidants, making it a potential drought-tolerant cultivar. Genotypic variation between these cultivars has potential for breeding drought-tolerant strawberries in the future.
Article
Chemistry, Physical
Hsean Ren Loi, Sahar Abbasiliasi, Pandian Bothi Raja, Mohd Shamzi Mohamed, Wen-Nee Tan, Hui Suan Ng, John Chi-Wei Lan, Joo Shun Tan
Summary: The present study aimed to optimize the nitrate reductase production in lactic acid bacteria (LAB) for enhanced production of silver nanoparticles (AgNPs) and characterize the biosynthesized AgNPs. Lactobacillus plantarum CAM 4 produced higher nitrate reductase activity under optimized medium and successfully enhanced the biosynthesis of AgNPs. The biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Biochemistry & Molecular Biology
Sangkaran Pannerchelvan, Leonardo Rios-Solis, Fadzlie Wong Faizal Wong, Uswatun Hasanah Zaidan, Helmi Wasoh, Mohd Shamzi Mohamed, Joo Shun Tan, Rosfarizan Mohamad, Murni Halim
Summary: Gamma-aminobutyric acid (GABA), a non-protein amino acid, has been extensively studied for its physiological functions and effects on metabolic disorders. Lactic acid bacteria (LAB) are important GABA producers due to their high GABA content and generally regarded as safe (GRAS) status. Biosynthesis of GABA through fermentation is preferred over chemical synthesis for its applications in food products. Optimization of fermentation conditions, such as enzyme activity, is crucial for maximizing GABA production.
Article
Biotechnology & Applied Microbiology
Najeeb Ullah, Mujaddad Ur Rehman, Abid Sarwar, Muhammad Nadeem, Rubina Nelofer, Hafiz Abdullah Shakir, Muhammad Irfan, Muhammad Idrees, Sumaira Naz, Ghulam Nabi, Sana Shah, Tariq Aziz, Metab Alharbi, Abdulrahman Alshammari, Faleh Alqahtani
Summary: This study focused on the purification, characterization, and application of alkaline proteases extracted from Bacillus cereus AUST-7. The purified enzyme showed stability and activity, making it a potential dehairing agent in the leather industry.
FERMENTATION-BASEL
(2022)
Article
Tropical Medicine
Nurul Hana Zainal Baharin, Nur Fadhilah Khairil Mokhtar, Mohd Nasir Mohd Desa, Nurul Diana Dzaraly, AbdulRahman Muthanna, Mazen M. Jamil Al-Obaidi, Mohd Hafis Yuswan, Sahar Abbasiliasi, Norasfaliza Rahmad, Wan Ahmad Kamil Wan Nur Ismah, Amalia Mohd Hashim, Shuhaimi Mustafa
Summary: This study investigated the inhibition mechanisms of secretome protein from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). The results showed that these secretome proteins reduce the growth of bacteria by damaging the cell membrane, leading to changes in morphology and internal composition.
ASIAN PACIFIC JOURNAL OF TROPICAL BIOMEDICINE
(2022)