Article
Virology
Alexandra Duev-Cohen, Batya Isaacson, Orit Berhani, Yoav Charpak-Amikam, Nehemya Friedman, Yaron Drori, Michal Mandelboim, Ofer Mandelboim
Summary: The study found that NKp46 can recognize influenza B viruses, but the interaction strength is weaker compared to influenza A, and NKp46 is not crucial for eliminating influenza B.
Letter
Oncology
Shoubao Ma, Michael A. Caligiuri, Jianhua Yu
Summary: This study identifies a four-stage developmental model for mouse NK cell development and defines two distinct populations of immature NK cells.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2022)
Article
Medicine, General & Internal
Boris V. Dons'koi, Serhiy M. Baksheev, Irina O. Sudoma, Ihor E. Palyha, Ksenia G. Khazhylenko, Dariia V. Zabara, Yaroslava I. Anoshko, Viktor E. Dosenko, Evgen I. Dubrovsky
Summary: This study investigated the expression of NKp46 in NK cells in the peripheral blood of women in early pregnancy and its association with pregnancy loss. The results showed that an imbalance in the NKp46(+) NK cell subpopulations was associated with an unfavorable ongoing pregnancy. A decreased level of NKp46(high) cells was strongly associated with miscarriage, while an increased level of the double-bright subpopulation was strongly associated with a successful pregnancy course.
Article
Biochemistry & Molecular Biology
Sheila B. Powers, Nourhan G. Ahmed, Roslin Jose, Marissa Brezgiel, Subhash Aryal, W. Paul Bowman, Porunelloor A. Mathew, Stephen O. Mathew
Summary: Acute lymphoblastic leukemia (ALL) is the most common pediatric cancer, with B-cell ALL being more prevalent and T-cell ALL being more aggressive. The study showed increased expression of LLT1 in B-ALL and T-ALL patients. Surface LLT1 expression was significantly increased in T cells, monocytes, and NK cells of ALL patients. The study also observed increased expression of CS1 and NKp46 on monocytes at the diagnosis of ALL. Post-induction chemotherapy, there was a decrease in LLT1, 2B4, CS1, and NKp46 expression on T cells of ALL patients. The findings suggest that the differential expression of these receptors/ligands may play a role in T-cell- and NK-cell-mediated immune surveillance of pediatric ALL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Antonio Arulanandam, Liang Lin, Hao-Ming Chang, Martine Cerutti, Sylvie Choblet, Peng Gao, Armin Rath, Armand Bensussan, Jean Kadouche, Daniel Teper, Ofer Mandelboim, Wei Li
Summary: GPC3 is highly expressed in multiple solid tumors and is barely expressed in adult normal tissues except the placenta. NKp46 activation receptor is expressed in all-natural killer cells, including tumor-infiltrating NK cells. CYT-303 is a multifunctional antibody that targets both GPC3 and NKp46 to mediate NK cell-redirected killing of HCC tumors.
Article
Cell Biology
Dae Yoon Kim, Jae Kwon Lee
Summary: The article investigates the changes in Natural Killer (NK) cell activity under diabetic conditions. The study finds that high glucose conditions influence NK cell activity, leading to reduced cytotoxic activity against cancer cells and decreased expression of activation markers.
CELLULAR IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Britta Lipinski, Paul Arras, Lukas Pekar, Daniel Klewinghaus, Ammelie Svea Boje, Simon Krah, Jasmin Zimmermann, Katja Klausz, Matthias Peipp, Vanessa Siegmund, Andreas Evers, Stefan Zielonka
Summary: In this study, potent NK cell engagers (NKCEs) targeting NKp46 and EGFR were developed using single domain antibodies (sdAbs) specific for NKp46 harboring the humanized Fab version of Cetuximab. Various VHH domains were obtained by immunization of camelids and yeast surface display. The resulting Fc effector-silenced NKCEs showed picomolar range potency in NK cell-mediated killing of EGFR-overexpressing tumor cells, which was further enhanced by co-engagement of Fc gamma receptor IIIa (Fc gamma RIIIa). The NKp46-specific sdAbs allowed the construction of different NKCE formats with favorable biophysical and biochemical properties, leading to significantly improved killing capacities.
Article
Immunology
Nilgun Sallakci, Ilhan Tahrali, Umut Can Kucuksezer, Esin Aktas Cetin, Ahmet Gul, Gunnur Deniz
Summary: This study investigated the activation status and function of NK cells in Behcet's disease patients. The results showed that patients in active and inactive phases displayed different activation levels of NK cells, indicating their potential association with immune attacks and remissions during the course of the disease.
IMMUNOLOGIC RESEARCH
(2022)
Review
Cell Biology
Philip Rosenstock, Thomas Kaufmann
Summary: Sialic acids play a crucial role in mediating the interaction between NK cells and target cells by influencing receptor activity and modulating NK cell function. NK cells can bind sialic acids on their surface, which in turn affects the activation or inhibition of NK cells during immune responses.
Article
Immunology
Maresa Lopez-Montano, Laura Jimenez-Ortega, Teresa Rocio Cruz-Hernandez, Victor Gabriel Hernandez-Chavez, Laura Arcelia Montiel-Cervantes, Elba Reyes-Maldonado, Jorge Vela-Ojeda
Summary: This study analyzed the role of NK cells, their activating receptors NKG2D and NKp46, and their ligands MIC-A and MIC-B, as well as soluble molecules sMIC-A and sMIC-B in dogs with Non-Hodkin's lymphoma. The study found that dogs with lymphoma had higher numbers of NK cells and increased levels of sMIC-A and sMIC-B.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2023)
Article
Immunology
Andrea Scheffschick, Sina Fuchs, Vivianne Malmstrom, Iva Gunnarsson, Hanna Brauner
Summary: This exploratory study provides the first characterization of NK cells and NK-like T-cells in lupus nephritis (LN) kidney, as well as their localization during active and inactive diseases. However, the role of NK cells in the pathogenesis of LN is still unclear and requires further studies.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2022)
Review
Virology
Jasmina M. Luczo, Sydney L. Ronzulli, Stephen M. Tompkins
Summary: NK cells are part of the innate immunity and function in recognizing and destroying tumorigenic and pathogen-infected cells through activation receptors. The activation receptors on NK cells include NKp46, NKp44, NKp30, NKG2D, and CD16.
Article
Immunology
Xiangqian Guan, Yuyan Lu, Yi Zhang, Ping Zhan, Zhigao Chen, Chuanzheng Wang, Zhenyu Yin
Summary: This study showed that early removal of NK cells can significantly inhibit tumor growth in a CD8+T cell-dependent manner in a murine hepatocellular carcinoma model. The presence of abundant NKp46+ NK cells in the tumor microenvironment was positively correlated with tumor growth and these cells exhibited dysfunction. Blockade of NKp46 effectively attenuated HCC growth. This work revealed a regulatory role of tumor-associated NK cells in HCC and suggested NKp46 as a potential target for HCC treatment.
IMMUNOLOGY LETTERS
(2023)
Article
Cell & Tissue Engineering
Junli Mou, Leling Xie, Yingxi Xu, Tong Zhou, Yu Liu, Qianqian Huang, Kejing Tang, Zheng Tian, Haiyan Xing, Shaowei Qiu, Qing Rao, Min Wang, Jianxiang Wang
Summary: This study establishes the involvement of the 2B4-CD48 axis in inhibiting apoptosis of NK cells through the p-ERK/BCL2 signaling pathway, leading to an improvement in therapeutic efficiency of NK cells.
Article
Parasitology
Xiaoyan Xu, Guangmei Zheng, Yushan Ren, Xiaohua He, Biwen Peng, Xuemei Hu, Wanhong Liu
Summary: Infections, particularly Toxoplasma gondii infection, can result in adverse pregnancy outcomes. This study discovered that the downregulation of 2B4 protein expression after T. gondii infection was associated with worse pregnancy outcomes. The interaction between 2B4 and signaling molecules such as SHP-2 and Fyn regulated the production of TNF-α and IFN-γ in decidual natural killer cells during T. gondii infection.
PARASITES & VECTORS
(2022)
Article
Hematology
Shlomo Elias, Inbal Kol, Shira Kahlon, Rajaa Amore, Mariam Zeibak, Dror Mevorach, Uriel Elchalal, Orly Zelig, Ofer Mandelboim
Summary: Anti-RhD antibodies are used to prevent RhD immunization, potentially by inducing NK cell degranulation. The mechanism involves CD16 activation and glycosylation of the antibodies. Additionally, the RhD drug may enhance killing of dendritic cells.
Article
Virology
Alexandra Duev-Cohen, Batya Isaacson, Orit Berhani, Yoav Charpak-Amikam, Nehemya Friedman, Yaron Drori, Michal Mandelboim, Ofer Mandelboim
Summary: The study found that NKp46 can recognize influenza B viruses, but the interaction strength is weaker compared to influenza A, and NKp46 is not crucial for eliminating influenza B.
Review
Immunology
Ariella Glasner, George Plitas
Summary: The immune system provides strong defense mechanisms against pathogens, but inadequate regulatory control can lead to various inflammatory disorders, including cancer. Treg cells play an important role in the tumor microenvironment, promoting tumor development by suppressing anti-tumor immune responses.
SEMINARS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Einat Seidel, Liat Dassa, Shira Kahlon, Boaz Tirosh, Anne Halenius, Tal Seidel Malkinson, Ofer Mandelboim
Summary: The study reveals that the glycoprotein US9 of human cytomegalovirus downregulates the activating immune ligand MICA*008 by slow cleavage of its signal peptide, leading to retention and degradation in the endoplasmic reticulum (ER) via the ER quality control system.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Einat Seidel, Liat Dassa, Corinna Schuler, Esther Oiknine-Djian, Dana G. Wolf, Vu Thuy Khanh Le-Trilling, Ofer Mandelboim
Summary: The study reveals that the uncharacterized HCMV protein UL147A interferes with the immune response by downregulating the expression of the immune ligand MICA*008, hindering the elimination of HCMV-infected cells. This finding advances the understanding of complex HCMV immune evasion mechanisms and may facilitate the development of antiviral drugs and vaccines.
Article
Immunology
Batya Isaacson, Maya Baron, Rachel Yamin, Gilad Bachrach, Francesca Levi-Schaffer, Zvi Granot, Ofer Mandelboim
Summary: CD300a plays a crucial role in the neutrophil-mediated immune response to UPEC-induced urinary tract infection. Deficiency of CD300a impairs phagocytic abilities of neutrophils, leading to severe exacerbation of infection.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Review
Dentistry, Oral Surgery & Medicine
S. Chaushu, Y. Klein, O. Mandelboim, Y. Barenholz, O. Fleissig
Summary: The review critically summarizes the immune processes involved in orthodontic tooth movement (OTM) inflammation and provides insights into the relationship between classical inflammation and clinical OTM phases. Recent studies highlight the existence of a typical innate immune response in OTM, with partial resolution observed in the lag phase. Future studies are needed to further uncover cellular and molecular immune targets for developing strategies to control OTM through modulation of the inflammatory process.
JOURNAL OF DENTAL RESEARCH
(2022)
Editorial Material
Allergy
Pier Giorgio Puzzovio, Ron Eliashar, Francesca Levi-Schaffer
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Review
Immunology
Pratibha Gaur, Ilan Zaffran, Tresa George, Fidan Rahimli Alekberli, Micha Ben-Zimra, Francesca Levi-Schaffer
Summary: This review summarizes updated information on the direct and indirect interactions of eosinophils with pathogens. Eosinophils, traditionally associated with allergic and parasitic diseases, have been shown to play a role in maintaining homeostasis and fighting infectious diseases. They possess various cellular mechanisms to combat pathogens and contribute to an efficient immune response.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2022)
Review
Allergy
Francesca Levi-Schaffer, Bernhard F. Gibbs, Jenny Hallgren, Carlo Pucillo, Frank Redegeld, Frank Siebenhaar, Joana Vitte, Soraya Mezouar, Moiese Michel, Pier Giorgio Puzzovio, Marcus Maurer
Summary: With the advancement of research technologies and methodologies, there has been an increased understanding of the origin, development, function, and interactions of human mast cells. The potential contribution of mast cells to disease has led to the development of novel therapeutic approaches.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yoav Charpak-Amikam, Tom Lapidus, Batya Isaacson, Alexandra Duev-Cohen, Tal Levinson, Adi Elbaz, Francesca Levi-Schaffer, Nir Osherov, Gilad Bachrach, Lois L. Hoyer, Maya Korem, Ronen Ben-Ami, Ofer Mandelboim
Summary: This study identifies how Candida albicans evades the immune response by manipulating the immune checkpoint receptor TIGIT. The results suggest that immunotherapy using a TIGIT-blocking antibody can restore anti-Candida immunity and serve as a potential therapeutic tool.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Ilan Zaffran, Nadine Landolina, Pratibha Gaur, Tihana Lenac Rovis, Jonjic Stipan, Ofer Mandelboim, Bernhard B. Singer, Francesca Levi-Schaffer
Summary: We investigated the effect of activating CEACAM1 on melanoma cells and found that it induced apoptosis. In vivo experiments also showed a slight inhibitory effect on tumor weight.
CANCER GENE THERAPY
(2022)
Correction
Biotechnology & Applied Microbiology
Ilan Zaffran, Nadine Landolina, Pratibha Gaur, Tihana Lenac Rovis, Stipan Jonjic, Ofer Mandelboim, Bernhard B. Singer, Francesca Levi-Schaffer
CANCER GENE THERAPY
(2023)
Review
Allergy
Martin Metz, Pavel Kolkhir, Sabine Altrichter, Frank Siebenhaar, Francesca Levi-Schaffer, Bradford A. Youngblood, Martin K. Church, Marcus Maurer
Summary: Chronic urticaria is a mast cell-dependent disease with limited therapeutic options. Current strategies focus on inhibiting IgE-mediated activation of mast cells and antagonizing the effects of released mediators. MC silencers have been developed as a new strategy to selectively block mast cell activation without causing immunosuppressive adverse effects.
Article
Immunology
Pratibha Gaur, Mansour Seaf, Nirit Trabelsi, Orly Marcu, Daria Gafarov, Ora Schueler-Furman, Ofer Mandelboim, Micha Ben-Zimra, Francesca Levi-Schaffer
Summary: This study confirms that the staphylococcus aureus exotoxin SEB can bind and activate the 2B4 receptor, exacerbating inflammation. 2B4 plays a crucial role in SA-associated inflammation and may serve as an interesting therapeutic target.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2023)