Rationally reduced libraries for combinatorial pathway optimization minimizing experimental effort

Employing a combinatorial expression approach to characterize xylose utilization in Saccharomyces cerevisiae

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Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria

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Functional optimization of gene clusters by combinatorial design and assembly

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Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly

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Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein

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Precise and reliable gene expression via standard transcription and translation initiation elements

(2013) Vivek K Mutalik et al.   NATURE METHODS

Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay

(2013) Michael E. Lee et al.   NUCLEIC ACIDS RESEARCH

Spanning high-dimensional expression space using ribosome-binding site combinatorics

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Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites

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Predictive design of mRNA translation initiation region to control prokaryotic translation efficiency

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Customized optimization of metabolic pathways by combinatorial transcriptional engineering

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The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes

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Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca

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Scalable Plasmid Transfer using Engineered P1-based Phagemids

(2012) Joshua T. Kittleson et al.   ACS Synthetic Biology

Microbial production of the drugs violacein and deoxyviolacein: analytical development and strain comparison

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Potential applications of violacein: a microbial pigment

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Rapid optimization of gene dosage in E. coli using DIAL strains

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RBSDesigner: software for designing synthetic ribosome binding sites that yields a desired level of protein expression

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Manufacturing Molecules Through Metabolic Engineering

(2010) J. D. Keasling   SCIENCE

You're one in a googol: optimizing genes for protein expression

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Diversity-based, model-guided construction of synthetic gene networks with predicted functions

(2009) Tom Ellis et al.   NATURE BIOTECHNOLOGY

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Design Parameters to Control Synthetic Gene Expression in Escherichia coli

(2009) Mark Welch et al.   PLoS One

Coding-Sequence Determinants of Gene Expression in Escherichia coli

(2009) G. Kudla et al.   SCIENCE

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Refinement and standardization of synthetic biological parts and devices

(2008) Barry Canton et al.   NATURE BIOTECHNOLOGY