Article
Biochemistry & Molecular Biology
Ghaith M. Hamza, Eric Miele, Don M. Wojchowski, Paul Toran, Camilla R. Worsfold, Tamil S. Anthonymuthu, Vladislav B. Bergo, Andrew X. Zhang, Jeffrey C. Silva
Summary: The Affi-BAMS™ epitope-directed affinity bead capture/MALDI MS platform is effective in quantitatively defining complex PTM marks of H3 and H4 histones. The platform achieves a range of >3 orders of magnitude with a technical precision CV of <5%. It can resolve heterogeneous histone N-terminal PTMs with as little as 100 μg of starting material.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Aneeqa Noor, Saima Zafar, Inga Zerr
Summary: Proteinopathy refers to disorders characterized by deposits of amyloid in tissues. Neurodegenerative proteinopathies, including Alzheimer's and Parkinson's diseases, involve proteins that may exist in multiple forms impacting disease prognosis differently. Understanding these proteoforms requires sensitive techniques like modified gel electrophoresis and mass spectrometry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Stanislav Naryzhny, Natalia Ronzhina, Elena Zorina, Fedor Kabachenko, Nikolay Klopov, Victor Zgoda
Summary: The use of tumor markers is important for early detection of cancer recurrence and prognosis. Finding ideal tumor markers that are sensitive, specific, and reliable is a crucial issue. Human plasma is a commonly used sample in clinics and provides noninvasive, rapid analysis for various diseases including cancer. Proteomics plays a major role in the search for ideal tumor markers. Classic plasma proteins, which are abundant, can be used to generate tumor biomarker profiles. These profiles should include information about protein levels and proteoform distribution. Future research involves generating profiles of these proteins in different cancers and developing test systems based on this information.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Kai Yao, Jing Zhang, Yumin Niu, Xin Zhang, Yunjia Yang, Yige Wu, Kai Wen, Bing Shao
Summary: The European Food Safety Authority (EFSA) has proposed a temporary tolerable daily intake of 0.04 ng/kg body weight/day for Bisphenol A (BPA) and its four analogues due to their potential endocrine disrupting effects. A more sensitive analytical method was urgently needed for the risk reassessment of bisphenols (BPs). The matrix effect of Chinese foods, which contain various spices, is a challenge for the analysis of ultra-trace analytes. In this study, a multi-immunoaffinity column (mIAC) combined with ultra-high-performance liquid chromatography tandem mass spectrometry detection (UHLPC-MS/MS) was used for the purification and analysis of BPA, BPB, BPF, BPS, and BPAF in Chinese foods.
Article
Chemistry, Analytical
Mojgan Sadat Shushtarian, Mohammad Hadi Givianrad, Mohammad Saber Tehrani, Parviz Aberoomand-Azar
Summary: This study developed an efficient strategy utilizing ion imprinted polymer (IIP) based mu-TLC-LA-ICP-MS for speciation analysis of inorganic arsenic. The IIP was characterized using FTIR and FESEM, and a TLC plate was successfully prepared. Under optimized conditions, the method demonstrated a low detection limit and wide linear dynamic range, with good performance in wastewater and human blood plasma samples.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Mei Han, Yunan Wang, Kevin Cook, Noor Bala, Marcus Soto, Dan A. Rock, Josh T. Pearson, Brooke M. Rock
Summary: As the pharmaceutical industry focuses more on pairing biological pathways with therapeutic intervention, the use of biologic drugs is increasing. Characterizing ADME properties is crucial for tuning the pharmacokinetic profiles of next generation biologics. The development of a robust IA-CE-MS workflow enables efficient characterization of a wide range of biotherapeutics and accelerates the drug development process.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
M. Sejalon-Cipolla, P. Bruyat, S. Bregant, C. Malgorn, L. Devel, G. Subra, S. Cantel
Summary: MALDI-MS is crucial for detecting and identifying molecules involved in biological pathways, but many biomarkers remain undetectable due to various limitations. To overcome these challenges, labeling strategies and activity-based probes have been developed. Matrix-free derivatization techniques are currently gaining attention.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Holly-May Lewis, Priyanka Gupta, Kyle D. G. Saunders, Shazneil Briones, Johanna von Gerichten, Paul A. Townsend, Eirini Velliou, Dany J. V. Beste, Olivier Cexus, Roger Webb, Melanie J. Bailey
Summary: This work presents a new approach for measuring drug levels and lipid fingerprints in individual living mammalian cells. The method combines nanocapillary sampling with liquid chromatography to improve precision in drug analysis. By optimizing the transfer of analytes from the sampling capillary, the researchers achieved efficient measurement of drug analytes in 30 living cells. They also discovered cell-to-cell heterogeneity in drug molecule uptake and identified lipid features associated with bedaquiline uptake.
Article
Chemistry, Analytical
Liang Shan, Han Gao, Jing Zhang, Wentao Li, Yue Su, Yinlong Guo
Summary: This study combined MALDI-TOF MS with EM technology to directly analyze exosomal proteins in human plasma and serum. The results showed that EM had higher sensitivity in the high-mass range and characteristic exosomal proteins were identified. Furthermore, when using EM as a detector, there was a good linear relationship between peak intensity and concentration.
Article
Biochemical Research Methods
Kai Liang, Qianqian Li, Zhijing Song, Keli Zhao, Rong Su, Shengchun Huang, Xueyan Guo, Yan Li
Summary: Non-cryopreservation temperature exposure can affect plasma quality, and changes in low-molecular-weight peptides can be used as biomarkers to predict sample quality. This study identified 10 peptides that consistently showed time-dependent changes, which can be used as biomarkers for quality control.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Medical Laboratory Technology
Lisa E. Kilpatrick, Roger Bouillon, W. Clay Davis, Clark M. Henderson, Andrew N. Hoofnagle, Steven Pauwels, Dirk Vanderschueren, Etienne Waelkens, Hans Wildiers, James H. Yen, Karen W. Phinney
Summary: This study investigated the influence of different proteoforms of vitamin D-binding protein (VDBP) on quantification using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results showed that VDBP proteoforms do not cause bias and a family of proteins can be accurately quantified using shared peptides. Assigning a reference value in a standard reference material can improve the accuracy of VDBP quantification in research and clinical samples.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Chemistry, Analytical
Zhenzhen Han, Cheng Peng, Jia Yi, Dongxue Zhang, Xiaowei Xiang, Xinyan Peng, Bin Su, Baohong Liu, Yuhui Shen, Liang Qiao
Summary: A size-dependent microfluidic chip was developed to efficiently isolate and purify exosomes from human blood, showing high protein cleaning efficiency and exosome recovery rate compared to traditional ultracentrifugation. The method uses symmetric layers of PMMA with a nanoporous PCTE membrane, allowing for quick and automated large-cohort clinical usage.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Food Science & Technology
Sandrine Livet, Sylvia Worbs, Herve Volland, Stephanie Simon, Martin B. Dorner, Francois Fenaille, Brigitte G. Dorner, Francois Becher
Summary: The study developed a MALDI-TOF mass spectrometry approach for fast and sensitive detection of abrin in food samples, providing a new method for analyzing the toxin.
Article
Biochemistry & Molecular Biology
Ilona Tornyi, Jozsef Lazar, Aladar Pettko-Szandtner, Eva Hunyadi-Gulyas, Laszlo Takacs
Summary: This article investigates the molecular heterogeneity of plasma C9 epitopes and their association with lung cancer. The study reveals three C9 epitopes with markedly different association with lung cancer and shows the lack of epitope interdependence or overlap. Epitope-specific immunoprecipitates of plasma C9 have identical peptide sequences but exhibit differences in posttranslational modification and coprecipitated protein composition with respect to control and lung cancer plasma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Chunxia Ma, Lei Xie, Xiao Wang, Kang Liang, Biao Kong
Summary: Functionalized mesoporous nanomaterials have diverse structural features and unique properties, making them widely applicable in MALDI-MS. Precisely controlling the factors in the assembly of mesoporous nanomaterials is crucial for achieving high-performance MALDI-MS. Significant progress has been made in the fabrication and properties of mesoporous nanomaterials in recent years.