‘Traceless’ tracing of proteins – high-affinity trans-splicing directed by a minimal interaction pair

Live-cell protein labelling with nanometre precision by cell squeezing

(2016) Alina Kollmannsperger et al.   Nature Communications

Recombinant Expression and Phenotypic Screening of a Bioactive Cyclotide Against α-Synuclein-Induced Cytotoxicity in Baker′s Yeast

(2015) Krishnappa Jagadish et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging

(2015) Ralph Wieneke et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Application of the Protein Semisynthesis Strategy to the Generation of Modified Chromatin

(2015) Matthew Holt et al.   Annual Review of Biochemistry

Photo-triggered fluorescent labelling of recombinant proteins in live cells

(2015) Deokho Jung et al.   CHEMICAL COMMUNICATIONS

Chemical tagging and customizing of cellular chromatin states using ultrafast trans-splicing inteins

(2015) Yael David et al.   Nature Chemistry

Click-Tag and Amine-Tag: Chemical Tag Approaches for Efficient Protein Labeling In Vitro and on Live Cells using the Naturally SplitNpuDnaE Intein

(2014) Vivien Schütz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Atypical Naturally Split Intein Engineered for Highly Efficient Protein Labeling

(2014) Ilka V. Thiel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Generation of a Genetically Encoded, Photoactivatable Intein for the Controlled Production of Cyclic Peptides

(2014) Jana K. Böcker et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Live-Cell Targeting of His-Tagged Proteins by Multivalent N-Nitrilotriacetic Acid Carrier Complexes

(2014) Ralph Wieneke et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structure-based engineering and comparison of novel split inteins for protein ligation

(2014) A. Sesilja Aranko et al.   Molecular BioSystems

Expression of Fluorescent Cyclotides using Protein Trans-Splicing for Easy Monitoring of Cyclotide-Protein Interactions

(2013) Krishnappa Jagadish et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Structure–activity studies on the upstream splice junction of a semisynthetic intein

(2013) Alexandra Wasmuth et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Site-specific N-terminal labeling of proteins using sortase-mediated reactions

(2013) Christopher S Theile et al.   Nature Protocols

A vector-free microfluidic platform for intracellular delivery

(2013) A. Sharei et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Inteins: nature's gift to protein chemists

(2013) Neel H. Shah et al.   Chemical Science

Recent progress in intein research: from mechanism to directed evolution and applications

(2012) Gerrit Volkmann et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

In-Cell Fluorescence Activation and Labeling of Proteins Mediated by FRET-Quenched Split Inteins

(2012) Radhika Borra et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions

(2012) Susanne A.I. Seidel et al.   METHODS

Kinetic Control of One-Pot Trans-Splicing Reactions by Using a Wild-Type and Designed Split Intein

(2011) Neel H. Shah et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Molecular Interaction Studies Using Microscale Thermophoresis

(2011) Moran Jerabek-Willemsen et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Highly Efficient and More Generalcis- andtrans-Splicing Inteins through Sequential Directed Evolution

(2011) Julia H. Appleby-Tagoe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Quantum-Yield-Optimized Fluorophores for Site-Specific Labeling and Super-Resolution Imaging

(2011) Christian Grunwald et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing

(2010) Nam Ky Chu et al.   JOURNAL OF PEPTIDE SCIENCE

A molecularly engineered split reporter for imaging protein-protein interactions with positron emission tomography

(2010) Tarik F Massoud et al.   NATURE MEDICINE

Protein-binding assays in biological liquids using microscale thermophoresis

(2010) Christoph J. Wienken et al.   Nature Communications

Segmental Isotopic Labeling of a Central Domain in a Multidomain Protein by ProteinTrans-Splicing Using Only One Robust DnaE Intein

(2009) Alena E. L. Busche et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Regulation and Cellular Roles of Ubiquitin-Specific Deubiquitinating Enzymes

(2009) Francisca E. Reyes-Turcu et al.   Annual Review of Biochemistry

Split Inteins as Versatile Tools for Protein Semisynthesis

(2009) Henning D. Mootz   CHEMBIOCHEM

Novel Split Intein fortrans-Splicing Synthetic Peptide onto C Terminus of Protein

(2009) Julia H. Appleby et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins

(2009) A. Sesilja Aranko et al.   PLoS One

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY