Article
Biochemistry & Molecular Biology
Chidozie C. Anyaegbu, Harrison Szemray, Sarah C. Hellewell, Nathan G. Lawler, Kerry Leggett, Carole Bartlett, Brittney Lins, Terence McGonigle, Melissa Papini, Ryan S. Anderton, Luke Whiley, Melinda Fitzgerald
Summary: Mild traumatic brain injury (mTBI) can cause structural, cellular, and biochemical changes in the brain that are difficult to detect and may persist chronically. Lipidomic analysis of blood samples, which can easily cross the blood-brain barrier, may provide valuable insights into the neuropathological state. This study used liquid chromatography-mass spectrometry (LC-MS) to analyze plasma lipid concentrations after 11 days of sham, one (1 x), or two (2 x) mTBI in rats. They identified 18 lipid species that distinguished between different injury conditions and found that the changes in lipid concentrations varied depending on the number of mTBI, suggesting a balance of damage and reparative responses.
Article
Behavioral Sciences
Maira Gloria de Freitas Cardoso, Joao Luis Vieira Monteiro de Barros, Rafael Alves Bonfim de Queiroz, Natalia Pessoa Rocha, Carlisa Silver, Agnes Stephanie da Silva, Ewelin Wasner Machado da Silva, Isadora Goncalves Roque, Julia de Lima Carvalho, Laura Ferreira dos Santos, Leticia Bitencourt Cota, Lucas Miranda Lemos, Mariana Figueiredo Miranda, Millena Figueiredo Miranda, Pedro Parenti Vianna, Rafael Arantes Oliveira, Tiago de Oliveira Furlam, Tulio Safar Sarquis Soares, Vinicius Sousa Pietra Pedroso, Rodrigo Moreira Faleiro, Erica Leandro Marciano Vieira, Antonio Lucio Teixeira, Leonardo Cruz de Souza, Aline Silva de Miranda
Summary: Very few studies have explored the cognition and impulsivity in the general population following mild traumatic brain injury (mTBI). The neurobiological mechanisms responsible for post-TBI neurobehavioral syndromes are complex and not well understood. In this study, machine learning modeling was used to investigate potential biomarkers of mTBI-associated impulsivity. The results showed that mTBI patients exhibited significantly higher levels of impulsivity compared to healthy controls, and certain blood biomarkers were associated with impulsivity in mTBI patients, suggesting different pathophysiological pathways involved in mTBI-related impulsivity.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Review
Clinical Neurology
Emma Clark, Saurab Faruque, Cedric Mutebi, Newton V. Nagirimadugu, Alyssa Kim, Malavika Mahendran, Elaine Sullo, Rajendra Morey, Robert W. Turner
Summary: This systematic review aimed to synthesize recent literature on the relationship between mild traumatic brain injury (mTBI) and Alzheimer's disease and related dementias (ADRD). Of the eight relevant articles identified, four showed a significant relationship between mTBI and disease outcomes, highlighting the need for future research on this causal relationship. Future studies should standardize definitions and methodologies to produce more reliable and comprehensive results.
JOURNAL OF NEUROLOGY
(2022)
Review
Clinical Neurology
Shazia Malik, Omar Alnaji, Mahnoor Malik, Teresa Gambale, Forough Farrokhyar, Michel P. Rathbone
Summary: Mild traumatic brain injuries (mTBIs) result in a neuroinflammatory response characterized by perturbations in inflammatory cytokines. A systematic review and meta-analysis synthesized data on the levels of inflammatory cytokines in mTBI patients. The analysis included 26 studies and found that IL-6, IL-1RA, IFN-gamma, and MCP-1/CCL2 levels were elevated in mTBI patients compared to healthy controls. Furthermore, certain cytokines were associated with poor clinical outcomes following mTBI. This research emphasizes the need for standardized methodologies and provides guidance for future mTBI studies.
FRONTIERS IN NEUROLOGY
(2023)
Article
Biology
Hanem Ellethy, Shekhar S. Chandra, Fatima A. Nasrallah
Summary: This study utilized data-driven models for diagnosing mild traumatic brain injury (mTBI) in a pediatric population using head CT scans. The results demonstrated that deep ANN and RF-ANN models showed high accuracy of 99% in identifying mTBI patients. This suggests the potential application of machine learning in diagnosing mTBI in pediatric populations.
COMPUTERS IN BIOLOGY AND MEDICINE
(2021)
Article
Clinical Neurology
Christina Devoto, Rany Vorn, Sara Mithani, Timothy B. Meier, Chen Lai, Steven P. Broglio, Thomas Mcallister, Christopher C. Giza, Daniel Huber, Jaroslaw Harezlak, Kenneth L. Cameron, Gerald Mcginty, Jonathan Jackson, Kevin Guskiewicz, Jason P. Mihalik, Alison Brooks, Stefan Duma, Steven Rowson, Lindsay D. Nelson, Paul Pasquina, Christine Turtzo, Lawrence Latour, Michael A. Mccrea, Jessica M. Gill, CARE Consortium Investigators
Summary: This study aimed to investigate plasma phosphorylated tau (p-tau181) protein in mild traumatic brain injury (mTBI) patients and concussed athletes. The study found that plasma p-tau181 concentrations were significantly elevated within 48 hours of injury in both groups, with the highest levels observed in patients with neuroimaging evidence of injury. These findings suggest that plasma p-tau181 concentrations may be related to brain injury and could potentially serve as an early diagnostic biomarker for mTBI/concussions.
FRONTIERS IN NEUROLOGY
(2023)
Article
Health Care Sciences & Services
Marquise M. Bonn, Laura J. Graham, Stephanie Marrocco, Samantha Jeske, Becky Moran, Dalton L. Wolfe
Summary: This study evaluates the user experiences and perceptions of the Parkwood Pacing and Planning & TRADE; application for individuals with mild traumatic brain injuries (mTBI), and provides recommendations for improvement. The results show that patients and clinicians have a positive experience with the application, but there is still room for improvement to enhance user experience.
Article
Clinical Neurology
Yvonn Kraemer, Kaisa Maki, Ivan Marinkovic, Taina Nybo, Harri Isokuortti, Antti Huovinen, Antti Korvenoja, Susanna Melkas, Hanna Harno
Summary: Post-traumatic headache (PTH) is a common symptom following mild traumatic brain injury (mTBI), with some patients developing persistent PTH (pPTH). This study found that risk factors for acute PTH (aPTH) include headache at the emergency department (ED), other pain, insomnia, and vertigo, while risk factors for pPTH include older age and current insomnia. Patients with pPTH had lower return to work rates.
JOURNAL OF HEADACHE AND PAIN
(2022)
Article
Biochemistry & Molecular Biology
Marcela Konjevod, Jorge Saiz, Matea Nikolac Perkovic, Gordana Nedic Erjavec, Lucija Tudor, Suzana Uzun, Oliver Kozumplik, Coral Barbas, Neven Zarkovic, Nela Pivac, Dubravka Svob Strac
Summary: Posttraumatic stress disorder (PTSD) is a complex neuropsychiatric disorder triggered by a traumatic event. This study used lipidomic analysis to identify altered lipid compounds in PTSD patients. The identified lipids are associated with biological processes such as membrane integrity and function, mitochondrial dysfunction, inflammation, and oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Neurosciences
William T. O'Brien, Louise Pham, Rhys D. Brady, Jesse Bain, Glenn R. Yamakawa, Mujun Sun, Richelle Mychasiuk, Terence J. O'Brien, Mastura Monif, Sandy R. Shultz, Stuart J. McDonald
Summary: The study demonstrates that serum NfL levels significantly increase following mTBI in rats, with a peak at 1 day post-injury and sustained elevation at 14 days. Repeat mTBI further elevates NfL levels, which are correlated with the severity of sensorimotor impairment. These findings highlight the potential utility of NfL as a blood biomarker for assessing the severity of mTBI.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eric C. Gier, Alexis N. Pulliam, David A. Gaul, Samuel G. Moore, Michelle C. LaPlaca, Facundo M. Fernandez
Summary: This research identified lipid biomarker candidates for traumatic brain injury (TBI) by analyzing serum samples from Sprague Dawley rats. Machine learning and feature selection approaches were used to construct lipid panels capable of distinguishing injured and uninjured rats with over 90% accuracy. These biomarker candidates were associated with various metabolic pathways.
Article
Psychiatry
Alice Theadom, Lisa Meehan, Sandra McCallum, Gail Pacheco
Summary: This study aims to determine whether people who have experienced a single or multiple mild traumatic brain injury (mTBI) have an increased risk of criminal behavior 10 years after the injury compared to matched orthopedic controls. The results showed that individuals who have experienced a single mTBI had significantly higher numbers of violent charges and convictions in the 10 years after the injury, while those with multiple mTBIs had higher violent charges and convictions in males but not in females.
FRONTIERS IN PSYCHIATRY
(2023)
Article
Immunology
Katie J. Neale, Hannah M. O. Reid, Barbara Sousa, Erin Mcdonagh, Jamie Morrison, Sandy Shultz, Eric Eyolfson, Brian R. Christie
Summary: Childhood is a critical time for brain growth and development, but it is also associated with an increased risk of mild traumatic brain injuries (mTBI). Using a closed head injury model, researchers investigated the acute effects of repeated mTBI on neurological function and cellular proliferation in juvenile rats. The study found that repeated mTBI did not lead to cumulative neurological deficits but did increase cellular proliferation in the hippocampus, particularly in male animals.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Nursing
Sarah R. Martha, Kuan-Fu Chen, Yvonne Lin, Hilaire J. Thompson
Summary: Metabolomics analysis revealed differences in phospholipid metabolism between older adults with mTBI and controls, which may be associated with long-term functional outcomes.
BIOLOGICAL RESEARCH FOR NURSING
(2021)
Article
Immunology
Tyler Nguyen, Natalie Nguyen, Ashlyn G. Cochran, Jared A. Smith, Mohammed Al-Juboori, Andrew Brumett, Saahil Saxena, Sarah Talley, Edward M. Campbell, Alexander G. Obukhov, Fletcher A. White
Summary: This study investigates the role of inflammation in long-term pain after mild traumatic brain injuries (mTBIs). Using a mouse model, the researchers found that inhibiting NLRP3 inflammasome activation can significantly reduce pain caused by mTBIs. These findings are important for understanding and treating chronic pain associated with mTBIs.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Multidisciplinary Sciences
Mehdi Eshraghi, Uri Nimrod Ramirez-Jarquin, Neelam Shahani, Tommaso Nuzzo, Arianna De Rosa, Supriya Swarnkar, Nicole Galli, Oscar Rivera, George Tsaprailis, Catherina Scharager-Tapia, Gogce Crynen, Qin Li, Marie-Laure Thiolat, Erwan Bezard, Alessandro Usiello, Srinivasa Subramaniam
Article
Chemistry, Analytical
Jie Zheng, Timothy S. Strutzenberg, Adrian Reich, Venkatasubramanian Dharmarajan, Bruce D. Pascal, Gogce C. Crynen, Scott J. Novick, Ruben D. Garcia-Ordonez, Patrick R. Griffin
ANALYTICAL CHEMISTRY
(2020)
Article
Clinical Neurology
Andrew Pearson, Rosa Ajoy, Gogce Crynen, Jon M. Reed, Moustafa Algamal, Michael Mullan, Dushyant Purohit, Fiona Crawford, Joseph O. Ojo
Article
Chemistry, Multidisciplinary
Hafeez S. Haniff, Laurent Knerr, Xiaohui Liu, Gogce Crynen, Jonas Bostrom, Daniel Abegg, Alexander Adibekian, Elizabeth Lekah, Kye Won Wang, Michael D. Cameron, Ilyas Yildirim, Malin Lemurell, Matthew D. Disney
Article
Biochemical Research Methods
Kyra Duong, Jiajia Ou, Zhaoliang Li, Zhaoqing Lv, Hao Dong, Tao Hu, Yunyun Zhang, Ava Hanna, Skyler Gordon, Gogce Crynen, Steven R. Head, Phillip Ordoukhanian, Yan Wang
Summary: A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. The limit of detection was further improved with the newly developed real-time dPCR technology through removal of false-positive signals, showing a larger linear dynamic range compared to end point dPCR.
Meeting Abstract
Gastroenterology & Hepatology
Catherine Martinez, Maria Alejandra Quintero Cusguen, Judith Pignac-Kobinger, Gogce Crynen, Irina Fernandez, Ana Santander, Amber Delmas, David Kerman, Oriana Damas, Amar Deshpande, Juan Burgueno, Maria Abreu, Mark Sundrud
INFLAMMATORY BOWEL DISEASES
(2021)
Article
Neurosciences
Jenna A. Levy, Christy W. LaFlamme, George Tsaprailis, Gogce Crynen, Damon T. Page
Summary: The study reveals that deletion of Dyrk1a gene in the cortex results in decreased brain mass, reduced neuronal size, structural hypoconnectivity, and autism-relevant behaviors in mice. Phosphoproteomic screening identified dysregulated growth-associated signaling cascades upon Dyrk1a deletion, and genetic suppression of Pten or pharmacological treatment with IGF-1 rescued microcephaly and neuronal undergrowth in mutant mice. These findings highlight a previously unknown mechanism through which Dyrk1a mutations disrupt growth factor signaling in the developing brain, influencing neuronal growth and connectivity.
BIOLOGICAL PSYCHIATRY
(2021)
Article
Geriatrics & Gerontology
Joseph O. Ojo, Jon M. Reed, Gogce Crynen, Prashanthi Vallabhaneni, James Evans, Benjamin Shackleton, Maximillian Eisenbaum, Charis Ringland, Anastasia Edsell, Michael Mullan, Fiona Crawford, Corbin Bachmeier
Summary: Cerebrovascular dysfunction and cerebral amyloid angiopathy are hallmark features of Alzheimer's disease. Molecular damage to cerebrovessels can lead to alterations in vascular clearance mechanisms and amyloid deposition. Proteomic analysis revealed significant changes in protein expression in cerebrovessels with aging and AD, with dysregulation of pathways related to chemokine signaling, HIF1 alpha, and mitochondrial function. These findings provide insights into the pathogenesis of AD and suggest potential therapeutic targets for improving cerebrovascular function.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Microbiology
Huihui Mou, Brian D. Quinlan, Haiyong Peng, Guanqun Liu, Yan Guo, Shoujiao Peng, Lizhou Zhang, Meredith E. Davis-Gardner, Matthew R. Gardner, Gogce Crynen, Lindsey B. DeVaux, Zhi Xiang Voo, Charles C. Bailey, Michael D. Alpert, Christoph Rader, Michaela U. Gack, Hyeryun Choe, Michael Farzan
Summary: The research suggests that pangolins may serve as an intermediate host between humans and bats, and that bat ACE2 orthologs could provide insights useful for enhancing the potency of ACE2-based therapeutics.
Article
Medicine, Research & Experimental
Mai H. Tran, Hajeung Park, Christopher L. Nobles, Pabalu Karunadharma, Li Pan, Guocai Zhong, Haimin Wang, Wenhui He, Tianling Ou, Gogce Crynen, Kelly Sheptack, Ian Stiskin, Huihui Mou, Michael Farzan
Summary: CRISPR effector proteins, such as Cas12a/Cpf1, can introduce double-stranded breaks into the mammalian genome to facilitate gene editing. In this study, researchers observed that a novel Cas12a ortholog, Lb2Cas12a, edited mammalian genes with efficiency comparable to well-characterized Cas12a orthologs. By introducing mutations into Lb2Cas12a, the researchers created a variant, Lb2-KY, that exhibited enhanced cleavage efficiency for a range of target sequences beyond those targeted by commonly used Cas12a orthologs. This Lb2-KY variant demonstrated efficient editing of hemoglobin target regions useful for correcting sickle-cell anemia, indicating its potential for modifying clinically relevant targets in the human genome.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Oncology
Jiachen Xu, Kyra Duong, Zhenlin Yang, Kavanaugh Kaji, Jiajia Ou, Steven R. Head, Gogce Crynen, Phillip Ordoukhanian, Lauren Hanna, Ava Hanna, Yan Wang, Zhijie Wang, Jie Wang
Summary: Real-time digital PCR improves sensitivity and accuracy by utilizing amplification curves, especially performing better at very low allele frequencies. It effectively reduces false negative results from tissue biopsy, allowing more patients to receive targeted therapy for better survival.
TRANSLATIONAL LUNG CANCER RESEARCH
(2021)
Article
Cell Biology
Jingru Fang, Colette Pietzsch, George Tsaprailis, Gogce Crynen, Kelvin Frank Cho, Alice Y. Ting, Alexander Bukreyev, Juan Carlos de la Torre, Erica Ollmann Saphire
Summary: This study utilized proximity proteomics and siRNA screening to identify the functional interactome of Ebola virus polymerase. It identified two cellular mRNA decay factors and demonstrated their role in promoting viral replication. Targeting one of these factors for degradation significantly reduced viral RNA load and particle production.
Article
Multidisciplinary Sciences
Jingru Fang, Colette Pietzsch, Haydar Witwit, George Tsaprailis, Gogce Crynen, Kelvin Frank Cho, Alice Y. Ting, Alexander Bukreyev, Erica Ollmann Saphire, Juan Carlos de la Torre
Summary: In this study, proximity proteomics was used to define the interactome of Lassa virus polymerase, leading to the identification of proteins involved in the virus replication and transcription. Eukaryotic peptide chain release factor subunit 3a was characterized as a proviral factor that physically associates with Lassa virus polymerase. Targeted degradation of this factor resulted in strong inhibition of Lassa virus infection, suggesting it as a potential target for antiviral development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Blessy M. Suresh, Yoshihiro Akahori, Amirhossein Taghavi, Gogce Crynen, Quentin M. R. Gibaut, Yue Li, Matthew D. Disney
Summary: By constructing a comprehensive RNA-focused small-molecule fragment collection and studying the binding landscape of different fragments to RNA experimentally, a potent and specific drug-like fragment targeting a specific RNA target was discovered, showing promising application in alleviating oncogenic features of certain cancer cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Neurosciences
Joseph O. Ojo, Gogce Crynen, Moustafa Algamal, Prashanti Vallabhaneni, Paige Leary, Benoit Mouzon, Jon M. Reed, Michael Mullan, Fiona Crawford