Germline Editing of Drosophila Using CRISPR-Cas9-based Cytosine and Adenine Base Editors

A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene editing in Drosophila

(2023) Roman M. Doll et al.   Science Advances

CRISPR–Cas9 gRNA efficiency prediction: an overview of predictive tools and the role of deep learning

(2022) Vasileios Konstantakos et al.   NUCLEIC ACIDS RESEARCH

PERIOD Phosphoclusters Control Temperature Compensation of the Drosophila Circadian Clock

(2022) Radhika Joshi et al.   Frontiers in Physiology

A novel period mutation implicating nuclear export in temperature compensation of the Drosophila circadian clock

(2022) Astrid Giesecke et al.   CURRENT BIOLOGY

Evolution of casein kinase 1 and functional analysis of new doubletime mutants in Drosophila

(2022) Nirav Thakkar et al.   Frontiers in Physiology

In vivo base editing rescues Hutchinson–Gilford progeria syndrome in mice

(2021) Luke W. Koblan et al.   NATURE

Low-temperature incubation improves both knock-in and knock-down efficiencies by the CRISPR/Cas9 system in Xenopus laevis as revealed by quantitative analysis

(2021) Sumika Kato et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A Simple Heat Treatment Increases SpCas9-Mediated Mutation Efficiency in Arabidopsis

(2021) Shuta Kurokawa et al.   PLANT AND CELL PHYSIOLOGY

Loss of Timeless Underlies an Evolutionary Transition within the Circadian Clock

(2021) Joanna Kotwica-Rolinska et al.   MOLECULAR BIOLOGY AND EVOLUTION

Base Editing of Somatic Cells Using CRISPR–Cas9 in Drosophila

(2021) Elizabeth Marr et al.   CRISPR Journal

Treatment of a Mouse Model of ALS by In Vivo Base Editing

(2020) Colin K.W. Lim et al.   MOLECULAR THERAPY

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

BE4max and AncBE4max Are Efficient in Germline Conversion of C:G to T:A Base Pairs in Zebrafish

(2020) Blake Carrington et al.   Cells

Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor

(2019) Florian Veillet et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Molecular safeguarding of CRISPR gene drive experiments

(2019) Jackson Champer et al.   eLife

Adenine base editing in an adult mouse model of tyrosinaemia

(2019) Chun-Qing Song et al.   Nature Biomedical Engineering

CRISPR/Cas9 Genome Editing Introduction and Optimization in the Non-model Insect Pyrrhocoris apterus

(2019) Joanna Kotwica-Rolinska et al.   Frontiers in Physiology

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

New Drosophila Circadian Clock Mutants Affecting Temperature Compensation Induced by Targeted Mutagenesis of Timeless

(2019) Samarjeet Singh et al.   Frontiers in Physiology

BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity

(2018) Wen Jiang et al.   CELL RESEARCH

Optimized Target-AID system efficiently induces single base changes in zebrafish

(2018) Xiaochan Lu et al.   Journal of Genetics and Genomics

Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

(2018) Luke W Koblan et al.   NATURE BIOTECHNOLOGY

Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing

(2018) Shouwei Tian et al.   PLANT CELL REPORTS

Reducing resistance allele formation in CRISPR gene drive

(2018) Jackson Champer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Programmable Single and Multiplex Base-Editing in Bombyx mori Using RNA-Guided Cytidine Deaminases

(2018) Yufeng Li et al.   G3-Genes Genomes Genetics

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

In vivo targeted single-nucleotide editing in zebrafish

(2018) Shingo Tanaka et al.   Scientific Reports

Precise A•T to G•C base editing in the zebrafish genome

(2018) Wei Qin et al.   BMC BIOLOGY

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Temperature effect on CRISPR-Cas9 mediated genome editing

(2017) Guanghai Xiang et al.   Journal of Genetics and Genomics

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

(2017) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Highly efficient RNA-guided base editing in mouse embryos

(2017) Kyoungmi Kim et al.   NATURE BIOTECHNOLOGY

Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress

(2017) Chantal LeBlanc et al.   PLANT JOURNAL

Evolution of circadian rhythms: from bacteria to human

(2017) Utpal Bhadra et al.   SLEEP MEDICINE

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity

(2017) Alexis C. Komor et al.   Science Advances

Fruit flies on the front line: the translational impact ofDrosophila

(2016) Norbert Perrimon et al.   Disease Models & Mechanisms

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs

(2016) Fillip Port et al.   NATURE METHODS

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells

(2016) Gaelen T Hess et al.   NATURE METHODS

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

GSK-3 and CK2 Kinases Converge on Timeless to Regulate the Master Clock

(2016) Deniz Top et al.   Cell Reports

Non-Mendelian Dominant Maternal Effects Caused by CRISPR/Cas9 Transgenic Components in Drosophila melanogaster

(2016) C.-C. Lin et al.   G3-Genes Genomes Genetics

Genetics on the Fly: A Primer on the Drosophila Model System

(2015) K. G. Hales et al.   GENETICS

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

(2015) Raj Chari et al.   NATURE METHODS

Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research

(2015) Fillip Port et al.   G3-Genes Genomes Genetics

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

(2014) Xuebing Wu et al.   NATURE BIOTECHNOLOGY

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

(2014) F. Port et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in Drosophila

(2013) Shu Kondo et al.   GENETICS

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

A New ImageJ Plug-in “ActogramJ” for Chronobiological Analyses

(2011) Benjamin Schmid et al.   JOURNAL OF BIOLOGICAL RHYTHMS

Post-Translational Regulation and Nuclear Entry of TIMELESS and PERIOD Are Affected in New timeless Mutant

(2011) T. Hara et al.   JOURNAL OF NEUROSCIENCE