Low-temperature incubation improves both knock-in and knock-down efficiencies by the CRISPR/Cas9 system in Xenopus laevis as revealed by quantitative analysis
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Title
Low-temperature incubation improves both knock-in and knock-down efficiencies by the CRISPR/Cas9 system in Xenopus laevis as revealed by quantitative analysis
Authors
Keywords
Xenopus laevis, CRISPR/Cas9, Knock-down, Knock-in, ICE analysis, Flow cytometry
Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 543, Issue -, Pages 50-55
Publisher
Elsevier BV
Online
2021-01-28
DOI
10.1016/j.bbrc.2020.11.038
References
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Note: Only part of the references are listed.- Simple embryo injection of long single-stranded donor templates with the CRISPR/Cas9 system leads to homology-directed repair in Xenopus tropicalis and Xenopus laevis
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