RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression

Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo

(2015) Xiaoying Bian et al.   BIOTECHNOLOGY AND BIOENGINEERING

Heterologous expression of an orphan NRPS gene cluster from Paenibacillus larvae in Escherichia coli revealed production of sevadicin

(2015) Ying Tang et al.   JOURNAL OF BIOTECHNOLOGY

Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2)

(2015) Jia Yin et al.   Scientific Reports

Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis

(2015) Yongxin Li et al.   Scientific Reports

Heterologous Production of Glidobactins/Luminmycins inEscherichia coliNissle Containing the Glidobactin Biosynthetic Gene Cluster fromBurkholderiaDSM7029

(2014) Xiaoying Bian et al.   CHEMBIOCHEM

Metagenomic small molecule discovery methods

(2014) Zachary Charlop-Powers et al.   CURRENT OPINION IN MICROBIOLOGY

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

(2014) Kazuya Yamanaka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-Titer Heterologous Production in E. coli of Lyngbyatoxin, a Protein Kinase C Activator from an Uncultured Marine Cyanobacterium

(2013) Sarah E. Ongley et al.   ACS Chemical Biology

In Vivo Evidence for a Prodrug Activation Mechanism during Colibactin Maturation

(2013) Xiaoying Bian et al.   CHEMBIOCHEM

Development of a broad-host synthetic biology toolbox for ralstonia eutropha and its application to engineering hydrocarbon biofuel production

(2013) Changhao Bi et al.   Microbial Cell Factories

Improved seamless mutagenesis by recombineering using ccdB for counterselection

(2013) Hailong Wang et al.   NUCLEIC ACIDS RESEARCH

Discovery of Cryptic Polyketide Metabolites from Dermatophytes Using Heterologous Expression in Aspergillus nidulans

(2013) Wen-Bing Yin et al.   ACS Synthetic Biology

Direct Cloning, Genetic Engineering, and Heterologous Expression of the Syringolin Biosynthetic Gene Cluster inE. colithrough Red/ET Recombineering

(2012) Xiaoying Bian et al.   CHEMBIOCHEM

Heterologous Expression and Genetic Engineering of the Tubulysin Biosynthetic Gene Cluster Using Red/ET Recombineering and Inactivation Mutagenesis

(2012) Yi Chai et al.   CHEMISTRY & BIOLOGY

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting

(2012) Jun Fu et al.   NATURE BIOTECHNOLOGY

Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting

(2011) Marta Nedelkova et al.   NUCLEIC ACIDS RESEARCH

Genetically Engineered Lipopeptide Antibiotics Related to A54145 and Daptomycin with Improved Properties

(2010) K. T. Nguyen et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Development of a Genetic System for Combinatorial Biosynthesis of Lipopeptides in Streptomyces fradiae and Heterologous Expression of the A54145 Biosynthesis Gene Cluster

(2010) D. C. Alexander et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Genomic basis for natural product biosynthetic diversity in the actinomycetes

(2009) Markus Nett et al.   NATURAL PRODUCT REPORTS

The impact of genomics on the exploitation of the myxobacterial secondary metabolome

(2009) Silke C. Wenzel et al.   NATURAL PRODUCT REPORTS

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Assembly and Heterologous Expression of the Coumermycin A1 Gene Cluster and Production of New Derivatives by Genetic Engineering

(2008) Manuel Wolpert et al.   CHEMBIOCHEM

Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

(2008) Natalay Kouprina et al.   Nature Protocols

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

(2008) Zengyi Shao et al.   NUCLEIC ACIDS RESEARCH

Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition

(2008) Jun Fu et al.   NUCLEIC ACIDS RESEARCH

Heterologous Expression And Genetic Engineering of the Phenalinolactone Biosynthetic Gene Cluster by Using Red/ET Recombineering

(2007) Tina M. Binz et al.   CHEMBIOCHEM