Efficient Protein Expression and Biosynthetic Gene Cluster Regulation in Bacillus subtilis Driven by a T7-BOOST System

CRISPR–dCas12a-mediated genetic circuit cascades for multiplexed pathway optimization

(2023) Yaokang Wu et al.   Nature Chemical Biology

An operator-based expression toolkit for Bacillus subtilis enables fine-tuning of gene expression and biosynthetic pathway regulation

(2022) Gang Fu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A genetic toolkit for efficient production of secretory protein in Bacillus subtilis

(2022) Yang Li et al.   BIORESOURCE TECHNOLOGY

An enzymatic colorimetric whole-cell biosensor for high-throughput identification of lysine overproducers

(2022) Jie Liu et al.   BIOSENSORS & BIOELECTRONICS

So you want to express your protein in Escherichia coli?

(2021) Aatir A. Tungekar et al.   Essays in Biochemistry

Metabolic engineering of Bacillus subtilis for enhancing riboflavin production by alleviating dissolved oxygen limitation

(2021) Jiajia You et al.   BIORESOURCE TECHNOLOGY

Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria

(2021) George M Taylor et al.   NUCLEIC ACIDS RESEARCH

Enhancing the production of γ-aminobutyric acid in Escherichia coli BL21 by engineering the enzymes of the regeneration pathway of the coenzyme factor pyridoxal 5’-phosphate

(2021) Ping Yu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Engineering of Corynebacterium glutamicum for high-level γ-aminobutyric acid production from glycerol by dynamic metabolic control

(2021) Liang Wei et al.   METABOLIC ENGINEERING

CAMERS‐B: CRISPR/Cpf1 Assisted Multiple‐genes Editing and Regulation System for Bacillus subtilis

(2020) Yaokang Wu et al.   BIOTECHNOLOGY AND BIOENGINEERING

CRISPRi-Guided Multiplexed Fine-Tuning of Metabolic Flux for Enhanced Lacto-N-neotetraose Production in Bacillus subtilis

(2020) Xiaomin Dong et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Recent Advances in Recombinant Protein Production by Bacillus subtilis

(2020) Kang Zhang et al.   Annual Review of Food Science and Technology

A wheat bran inducible expression system for the efficient production of α-L-arabinofuranosidase in Bacillus subtilis

(2020) Minghua Ji et al.   ENZYME AND MICROBIAL TECHNOLOGY

High-Throughput Screening of T7 Promoter Mutants for Soluble Expression of Cephalosporin C Acylase in E. coli

(2019) Zihao Nie et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

An Engineered B. subtilis Inducible Promoter System with over 10 000-Fold Dynamic Range

(2019) Sebastian M. Castillo-Hair et al.   ACS Synthetic Biology

Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in Bacillus subtilis

(2019) Yaokang Wu et al.   NUCLEIC ACIDS RESEARCH

Integrating T7 RNA Polymerase and Its Cognate Transcriptional Units for a Host-Independent and Stable Expression System in Single Plasmid

(2018) Xiao Liang et al.   ACS Synthetic Biology

Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production

(2016) Jing Fu et al.   Biotechnology for Biofuels

Production of specific-molecular-weight hyaluronan by metabolically engineered Bacillus subtilis 168

(2016) Peng Jin et al.   METABOLIC ENGINEERING

Whole-cell conversion of l-glutamic acid into gamma-aminobutyric acid by metabolically engineered Escherichia coli

(2016) Chongrong Ke et al.   SpringerPlus

Enhanced C30 carotenoid production in Bacillus subtilis by systematic overexpression of MEP pathway genes

(2015) Dan Xue et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A dual control mechanism synchronizes riboflavin and sulphur metabolism inBacillus subtilis

(2015) Danielle Biscaro Pedrolli et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Construction of a Super-Competent Bacillus subtilis 168 Using the PmtlA-comKS Inducible Cassette

(2015) Regine Rahmer et al.   Frontiers in Microbiology

Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras

(2014) David L. Shis et al.   ACS Synthetic Biology

A chromosomally encoded T7 RNA polymerase-dependent gene expression system forCorynebacterium glutamicum: construction and comparative evaluation at the single-cell level

(2014) Maike Kortmann et al.   Microbial Biotechnology

Gene Position More Strongly Influences Cell-Free Protein Expression from Operons than T7 Transcriptional Promoter Strength

(2013) Fabio Chizzolini et al.   ACS Synthetic Biology

The two-step biotransformation of monosodium glutamate to GABA by Lactobacillus brevis growing and resting cells

(2012) Ying Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Ribozyme-based insulator parts buffer synthetic circuits from genetic context

(2012) Chunbo Lou et al.   NATURE BIOTECHNOLOGY

Modular control of multiple pathways using engineered orthogonal T7 polymerases

(2012) Karsten Temme et al.   NUCLEIC ACIDS RESEARCH

Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements

(2011) Trang Thi Phuong Phan et al.   JOURNAL OF BIOTECHNOLOGY

Construction of Chromosomally Located T7 Expression System for Production of Heterologous Secreted Proteins in Bacillus subtilis

(2010) Po Ting Chen et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis

(2010) Xiao-Zhou Zhang et al.   Microbial Biotechnology