An operator-based expression toolkit for Bacillus subtilis enables fine-tuning of gene expression and biosynthetic pathway regulation

Violacein Targets the Cytoplasmic Membrane of Bacteria

(2019) Ana C. G. Cauz et al.   ACS Infectious Diseases

High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

(2019) Dandan Li et al.   Microbial Cell Factories

Bacterial gene control by DNA looping using engineered dimeric transcription activator like effector (TALE) proteins

(2018) Nicole A Becker et al.   NUCLEIC ACIDS RESEARCH

Multilevel Regulation of Bacterial Gene Expression with the Combined STAR and Antisense RNA System

(2018) Young Je Lee et al.   ACS Synthetic Biology

Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis

(2018) Dingyu Liu et al.   ACS Synthetic Biology

Engineering a Glucosamine-6-phosphate Responsive glmS Ribozyme Switch Enables Dynamic Control of Metabolic Flux in Bacillus subtilis for Overproduction of N-Acetylglucosamine

(2018) Tengfei Niu et al.   ACS Synthetic Biology

High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system

(2017) Kang Zhang et al.   Microbial Cell Factories

Emergent Properties in Complex Synthetic Bacterial Promoters

(2017) Lummy Maria Oliveira Monteiro et al.   ACS Synthetic Biology

Tunable Control of an Escherichia coli Expression System for the Overproduction of Membrane Proteins by Titrated Expression of a Mutant lac Repressor

(2017) Seong Keun Kim et al.   ACS Synthetic Biology

Tailoring Escherichia coli for the l-Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins

(2017) Anna Hjelm et al.   ACS Synthetic Biology

Maltose Utilization as a Novel Selection Strategy for Continuous Evolution of Microbes with Enhanced Metabolite Production

(2017) Shu-De Liu et al.   ACS Synthetic Biology

Construction of a novel, stable, food-grade expression system by engineering the endogenous toxin-antitoxin system in Bacillus subtilis

(2016) Sen Yang et al.   JOURNAL OF BIOTECHNOLOGY

Engineering an inducible gene expression system for Bacillus subtilis from a strong constitutive promoter and a theophylline-activated synthetic riboswitch

(2016) Wenjing Cui et al.   Microbial Cell Factories

Engineering of synthetic, stress-responsive yeast promoters

(2016) Arun S. Rajkumar et al.   NUCLEIC ACIDS RESEARCH

Enhancement of a high efficient autoinducible expression system in Bacillus subtilis by promoter engineering

(2016) Jintao Cheng et al.   PROTEIN EXPRESSION AND PURIFICATION

Development of a Synthetic Oxytetracycline-Inducible Expression System for Streptomycetes Using de Novo Characterized Genetic Parts

(2016) Weishan Wang et al.   ACS Synthetic Biology

A novel engineering tool in the Bacillus subtilis toolbox: inducer-free activation of gene expression by selection-driven promoter decryptification

(2015) Miriam Dormeyer et al.   MICROBIOLOGY-SGM

Amplification of small molecule-inducible gene expression via tuning of intracellular receptor densities

(2015) Baojun Wang et al.   NUCLEIC ACIDS RESEARCH

Dual transcriptional-translational cascade permits cellular level tuneable expression control

(2015) Rosa Morra et al.   NUCLEIC ACIDS RESEARCH

Engineering a Lysine-ON Riboswitch for Metabolic Control of Lysine Production in Corynebacterium glutamicum

(2015) Li-Bang Zhou et al.   ACS Synthetic Biology

Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota

(2015) Mark Mimee et al.   Cell Systems

Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein

(2014) Jingqi Chen et al.   BIOTECHNOLOGY LETTERS

Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin

(2014) Nobutaka Nakashima et al.   METABOLIC ENGINEERING

Auto-inducible expression system based on the SigB-dependent ohrB promoter in Bacillus subtilis

(2014) R. Panahi et al.   MOLECULAR BIOLOGY

Principles of genetic circuit design

(2014) Jennifer A N Brophy et al.   NATURE METHODS

Programmable bacteria detect and record an environmental signal in the mammalian gut

(2014) Jonathan W. Kotula et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthetic circuits integrating logic and memory in living cells

(2013) Piro Siuti et al.   NATURE BIOTECHNOLOGY

Amplifying Genetic Logic Gates

(2013) J. Bonnet et al.   SCIENCE

The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter

(2012) Anna A Toymentseva et al.   Microbial Cell Factories

Mechanism of promoter repression by Lac repressor–DNA loops

(2012) Nicole A. Becker et al.   NUCLEIC ACIDS RESEARCH

Self-Inducible Bacillus subtilis Expression System for Reliable and Inexpensive Protein Production by High-Cell-Density Fermentation

(2011) Marian Wenzel et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Development of an anhydrotetracycline-inducible gene expression system for solvent-producing Clostridium acetobutylicum: A useful tool for strain engineering

(2011) Hongjun Dong et al.   METABOLIC ENGINEERING

Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors

(2011) Kambiz Morabbi Heravi et al.   Microbial Cell Factories

Construction of Chromosomally Located T7 Expression System for Production of Heterologous Secreted Proteins in Bacillus subtilis

(2010) Po Ting Chen et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Characterization of a Mannose Utilization System in Bacillus subtilis

(2010) T. Sun et al.   JOURNAL OF BACTERIOLOGY

Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis

(2010) Su-Jin Lee et al.   JOURNAL OF BIOTECHNOLOGY

Side effects of chaperone gene co-expression in recombinant protein production

(2010) Mónica Martínez-Alonso et al.   Microbial Cell Factories

Development of a Bacillus subtilis expression system using the improved Pglv promoter

(2010) Yang M Ming et al.   Microbial Cell Factories

Identification of a Catabolite-Responsive Element Necessary for Regulation of the cry4A Gene of Bacillus thuringiensis subsp. israelensis

(2009) S. Kant et al.   JOURNAL OF BACTERIOLOGY

A fast, robust and tunable synthetic gene oscillator

(2008) Jesse Stricker et al.   NATURE