Article
Biochemistry & Molecular Biology
Zhiyong Liu, Zhonghua Sun, Hongyun Liu, Weipin Niu, Xin Wang, Na Liang, Yanfei Wang, Yaxin Shi, Li Xu, Wei Shi
Summary: This study used single-cell RNA sequencing to analyze gene expression patterns in endometrial tissues from patients with adenomyosis, revealing the involvement of cancer-related terms, cell motility, inflammation, cell proliferation, and angiogenesis in the progression of adenomyosis. Additionally, cell subpopulations with high copy number variation and tumour-like features were identified in ectopic lesions. The study also confirmed the epithelial-endothelial transition and formation of vasculogenic mimicry in adenomyosis, suggesting potential strategies for disease management.
CELL AND BIOSCIENCE
(2021)
Article
Cell Biology
Dimitrios Tsitsipatis, Jennifer L. L. Martindale, Krystyna Mazan-Mamczarz, Allison B. B. Herman, Yulan Piao, Nirad Banskota, Jen-Hao Yang, Linna Cui, Carlos Anerillas, Ming-Wen Chang, Mary Kaileh, Rachel Munk, Xiaoling Yang, Ceereena Ubaida-Mohien, Chee W. W. Chia, Ajoy C. C. Karikkineth, Linda Zukley, Jarod D'Agostino, Kotb Abdelmohsen, Nathan Basisty, Supriyo De, Luigi Ferrucci, Myriam Gorospe
Summary: We identified age-associated changes in the transcriptome, including coding and noncoding RNAs, of healthy human skin fibroblasts from 82 individuals across a wide age range. The differential abundance of 1437 coding and 1177 long noncoding RNAs was observed as a function of age. Transcription factors and RNA-binding proteins were implicated in regulating the expression of differentially abundant mRNAs and age-associated lncRNAs. These findings suggest that the identified transcripts may have potential as biomarkers and therapeutic targets for aging skin.
Article
Biochemistry & Molecular Biology
Zhangxiang Zhao, YingYing Guo, Yaoyao Liu, Lichun Sun, Bo Chen, Chengyu Wang, Tingting Chen, Yuquan Wang, Yawei Li, Qi Dong, Liqiang Ai, Ran Wang, Yunyan Gu, Xia Li
Summary: The study individualized lncRNA expression profiles for breast cancer using the LncRNA Individualization method, constructing an individualized differentially expressed lncRNA profile for breast cancer and investigating subtype-specific lncRNAs.
Article
Reproductive Biology
Bei-Bei Zhang, Meng-Xiao Li, Hong-Na Wang, Chao Liu, Yan-Yan Sun, Teng-He Ma
Summary: In this study, the expression of lncRNAs and mRNAs associated with ovarian follicle development in Taihang chickens was analyzed at the genome-wide level. The findings identified key pathways and regulatory factors related to follicle selection, providing a new foundation for future research on reproductive physiology in Taihang chickens and offering new opportunities to improve their laying performance.
Article
Endocrinology & Metabolism
Yi-yi Wang, Hua Duan, Sha Wang, Yong-jun Quan, Jun-hua Huang, Zheng-chen Guo
Summary: The study revealed that the overexpression of Talin1 may cooperate with beta-E-2 to stimulate ADS endometrial stromal cell proliferation and neovascularization, synergistically promoting the growth and survival of ectopic lesions.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Cell Biology
Yanli Yang, Wenshuai Xu, Ruo-Lan Xiang, Xinlun Tian, Kai-Feng Xu
Summary: Autoimmune pulmonary alveolar proteinosis (APAP) is a rare lung disease. Long noncoding RNAs (lncRNAs) play a role in the pathogenesis of APAP. Differentially expressed lncRNAs and mRNAs were identified in APAP patient samples, providing insights for future functional research.
Article
Biology
I. Ormenezi, A. Ribeiro-Silva, J. C. Rosa-e-Silva, J. Meola, F. J. Candido-dos-Reis, O. B. Poli-Neto
Summary: This study evaluated the expression of Dicer, Drosha, and Exportin-5 in the eutopic and ectopic endometrium of women with adenomyosis. The results showed lower Drosha expression in the eutopic endometrium of women with adenomyosis compared to women without the disease, and a correlation between Drosha expression in the ectopic and paired eutopic endometrium. No significant difference was observed in Dicer or Exportin expression.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2022)
Review
Immunology
Alison Maclean, Vanya Barzilova, Simran Patel, Faith Bates, Dharani K. Hapangama
Summary: Inflammation plays a role in the symptomatology and pathogenesis of adenomyosis. The invasion of endometrium into the myometrium due to injury at the endo-myometrial interface causes inflammation and leads to the formation of adenomyosis lesions. These lesions result in local inflammation, heavy menstrual bleeding, chronic pelvic pain, and subfertility. Immunological differences have been observed in the eutopic endometrium and adenomyotic lesions compared to healthy endometrium. The presence of immune cells and dysregulated inflammatory processes in adenomyosis provide insights into its pathogenesis and potential fertility-sparing treatments.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fang Xu, Liya Jiang, Qianwei Zhao, Zhibiao Zhang, Yixian Liu, Shuangshuang Yang, Mengdan Yu, Huiping Chen, Jianying Zhang, Jintao Zhang
Summary: This study analyzed HCC tumor and adjacent normal tissue samples using TMT-labeled tandem mass spectrometry and whole-transcriptome sequencing, identifying 977 differentially expressed proteins and 243 genes, and comprehensively evaluating gene-regulatory circuits in HCC. Bioinformatics analysis revealed involvement of certain miRNAs in regulating hub genes, while crucial coexpression and competing endogenous RNA interaction axes revealed potential functions of lncRNAs and circRNAs in HCC.
Article
Biotechnology & Applied Microbiology
Yanni Zhao, Ningning Zhou, Jiamin Ren, Wang Liu, Chuang Zhou, Xuefeng Chen, Jieyu Zhao, Juanjuan Cao, Jinfang Yang, Jie Han, Huan Liu
Summary: This study comprehensively investigates the regulatory roles of the new cell density-dependent sRNA Qrr4 in V. alginolyticus, revealing its significant effects on the physiology, virulence, and metabolism of the bacteria. The disturbance of multiple metabolic pathways, including phospholipid, nucleotide, carbohydrate, and amino acid metabolic pathways, provides novel insights into the potential mechanism by which mutation of qrr4 interferes with cellular energy homeostasis and modulates the characteristics of V. alginolyticus.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Valerie M. Stacey, Sulev Koks
Summary: This study identified differentially expressed long noncoding RNA (lncRNA) genes in samples from psoriasis patients through computational analysis. The differentially expressed genes were found to be associated with the pathogenesis of psoriasis and inflammatory skin diseases, as well as involved in cell cycle pathway and IFN-γ signaling pathway regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Mathematical & Computational Biology
Wenjun Lu, Hengchen Liu, Xin Zhang, Yujun Guo, Lu Liu, Tieyun Guo, Liqin Qu, Shulong Yang, Zhaozhu Li
Summary: Research identified an RNA regulatory mechanism associated with clear cell renal carcinoma (ccRCC) progression, establishing predictive survival models and a ceRNA network which may offer new avenues and potential biomarkers for clinical prognosis and treatment of ccRCC patients.
COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE
(2022)
Article
Genetics & Heredity
Jingxuan Li, Xueyan Zhao, Yanping Wang, Jiying Wang
Summary: This study sequenced the expression profiles of long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) in the longissimus dorsi (LD) muscle of Laiwu pigs to understand their role in the regulation of intramuscular fat (IMF) deposition. The results identified differentially expressed protein-coding genes and noncoding RNAs related to adipogenesis, suggesting their important roles in regulating IMF deposition. A connective network was also constructed, revealing miRNAs that may affect lipid metabolism or adipogenesis. This work provides valuable information for further research on the functions of lncRNAs and circRNAs in IMF deposition.
Article
Plant Sciences
Nan Wu, Jun Yang, Guoning Wang, Huifeng Ke, Yan Zhang, Zhengwen Liu, Zhiying Ma, Xingfen Wang
Summary: This study used transcriptome sequencing to analyze the response of cotton fiber to water deficit and identified differentially expressed genes and lncRNAs that significantly affect fiber quality during development.
Article
Plant Sciences
Yaqi Jia, Huimin Zhao, Yani Niu, Yucheng Wang
Summary: Using RNA-seq, we identified salt-responsive genes and lncRNAs in Betula platyphylla Suk. roots and leaves, and characterized their functions. The salt-responsive genes were enriched in cell wall biogenesis and wood development in roots, and in photosynthesis and response to stimulus in leaves. The target genes of salt-responsive lncRNAs were enriched in nitrogen compound metabolic process and response to stimulus in both roots and leaves. We also developed a method for quickly identifying abiotic stress tolerance of lncRNAs, and characterized 11 randomly selected salt-responsive lncRNAs using gain- and loss-of-function analysis. Among them, six lncRNAs confer salt tolerance, two lncRNAs confer salt sensitivity, and three lncRNAs are not involved in salt tolerance. Furthermore, we characterized a lncRNA, LncY1, which improves salt tolerance by regulating two transcription factors, BpMYB96 and BpCDF3.
HORTICULTURE RESEARCH
(2023)
Article
Biochemical Research Methods
Cong Shen, Mingrui Li, Pan Zhang, Yueshuai Guo, Hao Zhang, Bo Zheng, Hui Teng, Tao Zhou, Xuejiang Guo, Ran Huo
MOLECULAR & CELLULAR PROTEOMICS
(2018)
Review
Biochemistry & Molecular Biology
Chao Liu, Yanjie Ma, Yongliang Shang, Ran Huo, Wei Li
CELLULAR AND MOLECULAR LIFE SCIENCES
(2018)
Article
Immunology
Chao Chen, Sulan Zhai, Le Zhang, Jingjing Chen, Xuehui Long, Jun Qin, Jianhua Li, Ran Huo, Xiaoming Wang
JOURNAL OF EXPERIMENTAL MEDICINE
(2018)
Article
Biochemistry & Molecular Biology
Cheng Wang, Yayun Gu, Erbao Zhang, Kai Zhang, Na Qin, Juncheng Dai, Meng Zhu, Jia Liu, Kaipeng Xie, Yue Jiang, Xuejiang Guo, Mingxi Liu, Guangfu Jin, Hongxia Ma, Tao Jiang, Rong Yin, Yankai Xia, Li Liu, Shouyu Wang, Bin Shen, Ran Huo, Lin Xu, Jiahao Sha, Bin Qu, Hongbing Shen, Zhibin Hu
Article
Biochemistry & Molecular Biology
Yumeng Cao, Mingrui Li, Fei Liu, XiaoBei Ni, Shuai Wang, Hao Zhang, Xuesong Sui, Ran Huo
Article
Cell Biology
Xuesong Sui, Yue Hu, Chao Ren, Qiqi Cao, Shuai Zhou, Yumeng Cao, Mingrui Li, Wenjie Shu, Ran Huo
Article
Biochemistry & Molecular Biology
Yue Hu, Zhangyi Ouyang, Xuesong Sui, Meijie Qi, Mingrui Li, Yuanlin He, Yumeng Cao, Qiqi Cao, Qianneng Lu, Shuai Zhou, Lu Liu, Li Liu, Bin Shen, Wenjie Shu, Ran Huo
CELL DEATH AND DIFFERENTIATION
(2020)
Article
Cell Biology
Qiqi Cao, Chun Zhao, Xiaolan Zhang, Heng Zhang, Qianneng Lu, Congjing Wang, Yue Hu, Xiufeng Ling, Junqiang Zhang, Ran Huo
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2020)
Article
Cell Biology
Qiqi Cao, Chun Zhao, Congjing Wang, Lingbo Cai, Meng Xia, Xiaolan Zhang, Jian Han, Yangyang Xu, Junqiang Zhang, Xiufeng Ling, Xiang Ma, Ran Huo
Summary: Mutations in PATL2, such as PATL2(Y217N), disrupt oocyte maturation by reducing ubiquitination levels and affecting translational regulation of Mos mRNA, leading to impaired MAPK signaling pathway and meiosis in oocytes. This highlights a new mechanism of action for PATL2 in maternal mRNA translation regulation during oocyte meiotic maturation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Cheng Wang, Hong Lv, Xiufeng Ling, Hong Li, Feiyang Diao, Juncheng Dai, Jiangbo Du, Ting Chen, Qi Xi, Yang Zhao, Kun Zhou, Bo Xu, Xiumei Han, Xiaoyu Liu, Meijuan Peng, Congcong Chen, Shiyao Tao, Lei Huang, Cong Liu, Mingyang Wen, Yangqian Jiang, Tao Jiang, Chuncheng Lu, Wei Wu, Di Wu, Minjian Chen, Yuan Lin, Xuejiang Guo, Ran Huo, Jiayin Liu, Hongxia Ma, Guangfu Jin, Yankai Xia, Jiahao Sha, Hongbing Shen, Zhibin Hu
Summary: Children conceived through assisted reproductive technology (ART) carry more germline de novo mutations (gDNMs) than spontaneously conceived children, primarily originating from fathers. Infertility of the father is associated with elevated paternal DNMs in ART offspring, while the use of specific drugs triggers an increase in maternal DNMs. Therefore, evaluating the germline status of fathers in ART families is crucial.
Article
Developmental Biology
Shuai Zhou, Yueshuai Guo, Haifeng Sun, Lu Liu, Liping Yao, Chao Liu, Yuanlin He, Shanren Cao, Cheng Zhou, Mingrui Li, Yumeng Cao, Congjing Wang, Qianneng Lu, Wei Li, Xuejiang Guo, Ran Huo
Summary: The study reveals that maternal RNF114 plays a crucial role in degrading important substrates during the maternal-to-zygotic transition, regulating the activation of major ZGA in mouse embryos. Misregulation of this process may impede appropriate ZGA activation.
Article
Obstetrics & Gynecology
Qianneng Lu, Xiaolan Zhang, Qiqi Cao, Congjing Wang, Jie Ding, Chun Zhao, Junqiang Zhang, Xiufeng Ling, Qingxia Meng, Ran Huo, Hong Li
Summary: In this study, five heterozygous variants of the TUBB8 gene were identified in infertile patients, with two of the variants confirmed to be associated with infertility phenotypes through in vitro experiments. The results expand the range of TUBB8 variants and phenotypic spectrum related to female infertility.
REPRODUCTIVE SCIENCES
(2021)
Article
Cell Biology
Mingrui Li, Chao Ren, Shuai Zhou, Yuanlin He, Yueshuai Guo, Hao Zhang, Lu Liu, Qiqi Cao, Congjing Wang, Jie Huang, Yue Hu, Xue Bai, Xuejiang Guo, Wenjie Shu, Ran Huo
Summary: The study found that abnormal expression of splicing regulation proteins in aged oocytes affects the splicing of DNA repair-related genes in 2-cell embryos, reducing their developmental potential.
Article
Obstetrics & Gynecology
Lu Liu, Jie Huang, Anlan He, Shuai Zhou, Qianneng Lu, Xiaoning Yu, Xue Bai, Mingrui Li, Ran Huo
Summary: This study found that 4,4'-dimethoxychalcone (DMC) can improve the quality of postovulatory ageing (POA) oocytes by eliminating accumulated reactive oxygen species (ROS), reducing apoptosis, improving mitochondrial distribution, and restoring the actin cap formation. DMC supplementation activates autophagy to protect oocytes from the effects of POA, leading to improved fertilization and developmental potential.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Biochemistry & Molecular Biology
Cheng Wang, Yayun Gu, Jun Zhou, Jie Zang, Xiufeng Ling, Hong Li, Lingmin Hu, Bei Xu, Bo Zhang, Na Qin, Hong Lv, Weiwei Duan, Yue Jiang, Yuanlin He, Tao Jiang, Congcong Chen, Xiumei Han, Kun Zhou, Bo Xu, Xiaoyu Liu, Shiyao Tao, Yangqian Jiang, Jiangbo Du, Juncheng Dai, Feiyang Diao, Chuncheng Lu, Xuejiang Guo, Ran Huo, Jiayin Liu, Yuan Lin, Yankai Xia, Guangfu Jin, Hongxia Ma, Hongbing Shen, Zhibin Hu
Summary: This study investigates the association between assisted reproductive technology (ART) and initial leukocyte telomere length (LTL) using whole-genome sequencing (WGS) data. The study finds that one-year-old children conceived by ART have shorter LTLs, especially in those conceived through blastocyst-stage embryo transfer. Mouse experiments also confirm that blastocyst-stage embryo transfer results in shorter telomere lengths.
