Process intensification for the production of a C-tagged antimicrobial peptide in Escherichia coli – First steps toward a platform technology

GbpA as a secretion and affinity purification tag for an antimicrobial peptide produced in Vibrio natriegens

(2022) Stefan Schwarz et al.   ELECTRONIC JOURNAL OF BIOTECHNOLOGY

Fusion Tag Design Influences Soluble Recombinant Protein Production in Escherichia coli

(2022) Christoph Köppl et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Fundamental insights in early‐stage inclusion body formation

(2022) Julian Kopp et al.   Microbial Biotechnology

Fusion tags to enhance heterologous protein expression

(2020) Mi-Ran Ki et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Recombinant protein production associated growth inhibition results mainly from transcription and not from translation

(2020) Zhaopeng Li et al.   Microbial Cell Factories

Characterization and comparison of two peptide-tag specific nanobodies for immunoaffinity chromatography

(2020) Jun Ren et al.   JOURNAL OF CHROMATOGRAPHY A

Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry

(2020) Mohammad Mahmoudi Gomari et al.   BIOTECHNOLOGY ADVANCES

The NT11, a novel fusion tag for enhancing protein expression in Escherichia coli

(2019) Thi Khoa My Nguyen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

An efficient expression tag library based on self-assembling amphipathic peptides

(2019) Weixin Zhao et al.   Microbial Cell Factories

Process Intensification for an Insect Antimicrobial Peptide Elastin-Like Polypeptide Fusion Produced in Redox-Engineered Escherichia coli

(2019) Mathias Joachim et al.   Frontiers in Bioengineering and Biotechnology

Selection of High Producers From Combinatorial Libraries for the Production of Recombinant Proteins in Escherichia coli and Vibrio natriegens

(2019) Joel Eichmann et al.   Frontiers in Bioengineering and Biotechnology

High yield recombinant expression and purification of oncogenic NSD1, NSD2, and NSD3 with human influenza hemagglutinin tag

(2019) Yunpeng Shen et al.   PROTEIN EXPRESSION AND PURIFICATION

A multifunctional tag with the ability to benefit the expression, purification, thermostability and activity of recombinant proteins

(2018) Weixin Zhao et al.   JOURNAL OF BIOTECHNOLOGY

Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags

(2018) Sarah C. Bernier et al.   PROTEIN EXPRESSION AND PURIFICATION

Production, quality control, stability, and potency of cGMP-produced Plasmodium falciparum RH5.1 protein vaccine expressed in Drosophila S2 cells

(2018) Jing Jin et al.   npj Vaccines

SKIK-zipbody-alkaline phosphatase, a novel antibody fusion protein expressed in Escherichia coli cytoplasm

(2018) Panwad Ritthisan et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

The therapeutic potential of the insect metalloproteinase inhibitor against infections caused by Pseudomonas aeruginosa

(2018) Michaela Eisenhardt et al.   JOURNAL OF PHARMACY AND PHARMACOLOGY

Downstream processing of Cry4AaCter-induced inclusion bodies containing insect-derived antimicrobial peptides produced in Escherichia coli

(2018) Daniel Hoffmann et al.   PROTEIN EXPRESSION AND PURIFICATION

Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment: A review

(2018) Yujie Ben et al.   ENVIRONMENTAL RESEARCH

Accelerating the clinical development of protein-based vaccines for malaria by efficient purification using a four amino acid C-terminal ‘C-tag’

(2017) Jing Jin et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

N-terminal SKIK peptide tag markedly improves expression of difficult-to-express proteins in Escherichia coli and Saccharomyces cerevisiae

(2017) Teruyo Ojima-Kato et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

A high-throughput expression screening platform to optimize the production of antimicrobial peptides

(2017) Christine Schreiber et al.   Microbial Cell Factories

Insect antimicrobial peptides: potential tools for the prevention of skin cancer

(2016) Miray Tonk et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of an insect metalloproteinase inhibitor drug carrier system for application in chronic wound infections

(2015) Michaela Eisenhardt et al.   JOURNAL OF PHARMACY AND PHARMACOLOGY

Challenges and recent advances in affinity purification of tag-free proteins

(2014) Dongli Guan et al.   BIOTECHNOLOGY LETTERS

Considerations for the process development of insect-derived antimicrobial peptide production

(2014) Hagen Müller et al.   BIOTECHNOLOGY PROGRESS

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system

(2014) Sofia Costa et al.   Frontiers in Microbiology

Seven Ways to Preserve the Miracle of Antibiotics

(2013) J. G. Bartlett et al.   CLINICAL INFECTIOUS DISEASES

Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation

(2013) Tony Collins et al.   Microbial Cell Factories

E6 protein of human papillomavirus 16 (HPV16) expressed in Escherichia coli sans a stretch of hydrophobic amino acids, enables purification of GST-ΔE6 in the soluble form and retains the binding ability to p53

(2013) Ravi Ranjan Verma et al.   PROTEIN EXPRESSION AND PURIFICATION

Several Affinity Tags Commonly Used in Chromatographic Purification

(2013) Xinyu Zhao et al.   Journal of Analytical Methods in Chemistry

ANTI-infective Therapeutics from the Lepidopteran Model Host Galleria mellonella

(2012) Andreas Vilcinskas   CURRENT PHARMACEUTICAL DESIGN

Optimization of an extracellular zinc-metalloprotease (SVP2) expression in Escherichia coli BL21 (DE3) using response surface methodology

(2012) Laleh Beigi et al.   PROTEIN EXPRESSION AND PURIFICATION

An overview of enzymatic reagents for the removal of affinity tags

(2011) David S. Waugh   PROTEIN EXPRESSION AND PURIFICATION

Structure and Properties of a Complex of α-Synuclein and a Single-Domain Camelid Antibody

(2010) Erwin J. De Genst et al.   JOURNAL OF MOLECULAR BIOLOGY

Augmented biosynthesis of cadmium sulfide nanoparticles by genetically engineered Escherichia coli

(2009) Yen-Lin Chen et al.   BIOTECHNOLOGY PROGRESS