Advancements in the use of xenopus oocytes for modelling neurological disease for novel drug discovery

Functional Characterization of a Spectrum of Novel Romano-Ward Syndrome KCNQ1 Variants

(2023) Susanne Rinné et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies

(2023) Esther Henriet et al.   BRAIN

A draft human pangenome reference

(2023) Wen-Wei Liao et al.   NATURE

Gene variant effects across sodium channelopathies predict function and guide precision therapy

(2022) Andreas Brunklaus et al.   BRAIN

Development and characterization of functional antibodies targeting NMDA receptors

(2022) Nami Tajima et al.   Nature Communications

Trafficking of the glutamate transporter is impaired in LRRK2-related Parkinson’s disease

(2022) Ludovica Iovino et al.   ACTA NEUROPATHOLOGICA

Characterization of Aripiprazole Uptake Transporter in the Blood-Brain Barrier Model hCMEC/D3 Cells by Targeted siRNA Screening

(2022) Moeno Kadoguchi et al.   PHARMACEUTICAL RESEARCH

Novel Cell-Based Assay for Alpha-3 Nicotinic Receptor Antibodies Detects Antibodies Exclusively in Autoimmune Autonomic Ganglionopathy

(2022) Katerina Karagiorgou et al.   Neurology-Neuroimmunology & Neuroinflammation

Mammalian Brain Ca2+ Channel Activity Transplanted into Xenopus laevis Oocytes

(2022) Matthieu Rousset et al.   Membranes

Precision therapy with quinidine of KCNT1‐related epileptic disorders: a systematic review

(2022) Da Xu et al.   BRITISH JOURNAL OF CLINICAL PHARMACOLOGY

De novo KCNA6 variants with attenuated K V 1 .6 channel deactivation in patients with epilepsy

(2022) Vincenzo Salpietro et al.   EPILEPSIA

Xenopus Oocytes as a Powerful Cellular Model to Study Foreign Fully-Processed Membrane Proteins

(2022) Isabel Ivorra et al.   Membranes

Microtransplantation of Postmortem Native Synaptic mGluRs Receptors into Xenopus Oocytes for Their Functional Analysis

(2022) Brice Miller et al.   Membranes

Late onset sensory-motor axonal neuropathy, a novel SLC12A6 related phenotype

(2022) Sissel Løseth et al.   BRAIN

GRIN2A-related epilepsy and speech disorders: A comprehensive overview with a focus on the role of precision therapeutics

(2022) Debopam Samanta   EPILEPSY RESEARCH

Leucine 434 is essential for docosahexaenoic acid–induced augmentation of L-glutamate transporter current

(2022) Kanako Takahashi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

New use for an old drug: quinidine in KCNT1-related epilepsy therapy

(2022) Ru Liu et al.   NEUROLOGICAL SCIENCES

GABAergic Neurotransmission in Human Tissues Is Modulated by Cannabidiol

(2022) Gabriele Ruffolo et al.   Life-Basel

Efficient expression of concatenated α1β2δ and α1β3δ GABA A receptors, their pharmacology and stoichiometry

(2021) Vivian Wan Yu Liao et al.   BRITISH JOURNAL OF PHARMACOLOGY

Can rodent models manifest pathomechanisms of genetic epilepsy?

(2021) Motohiro Okada   BRITISH JOURNAL OF PHARMACOLOGY

An engineered membrane-bound guanylyl cyclase with light-switchable activity

(2021) Yuehui Tian et al.   BMC BIOLOGY

Cation leak underlies neuronal excitability in an HCN1 developmental and epileptic encephalopathy

(2021) Lauren E Bleakley et al.   BRAIN

Dopamine D2 Receptor Agonist Binding Kinetics—Role of a Conserved Serine Residue

(2021) Richard Ågren et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Light-regulated voltage-gated potassium channels for acute interrogation of channel function in neurons and behavior

(2021) Henry H. Jerng et al.   PLoS One

Pharmacological modulation of GABAA receptors

(2021) Frédéric Knoflach et al.   CURRENT OPINION IN PHARMACOLOGY

Biophysical analysis of an HCN1 epilepsy variant suggests a critical role for S5 helix Met-305 in voltage sensor to pore domain coupling

(2021) Andrew Hung et al.   PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

Recurrent seizure‐related GRIN1 variant: Molecular mechanism and targeted therapy

(2021) Yuchen Xu et al.   Annals of Clinical and Translational Neurology

PACmn for improved optogenetic control of intracellular cAMP

(2021) Shang Yang et al.   BMC BIOLOGY

Precision treatment with nicotine in autosomal dominant sleep-related hypermotor epilepsy (ADSHE): An observational study of clinical outcome and serum cotinine levels in 17 patients

(2021) Eylert Brodtkorb et al.   EPILEPSY RESEARCH

A missense mutation converts the Na+,K+-ATPase into an ion channel and causes therapy-resistant epilepsy

(2021) Sofia Ygberg et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Comparison of the clinical syndromes of anti-GABAa versus anti-GABAb associated autoimmune encephalitis: A systematic review

(2021) Nathalia Rossoni Ronchi et al.   JOURNAL OF NEUROIMMUNOLOGY

Praxisrelevante autoimmune Erkrankungen des zentralen Nervensystems in der Pädiatrie: frühe Diagnose und adäquater Therapiebeginn

(2021) Astrid Blaschek et al.   NERVENARZT

Nicotine Patch Improved Autosomal Dominant Sleep-Related Hypermotor Epilepsy

(2021) Sang M. Nguyen et al.   PEDIATRIC NEUROLOGY

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels

(2021) Kasper B. Hansen et al.   PHARMACOLOGICAL REVIEWS

Loss-of-function BK channel mutation causes impaired mitochondria and progressive cerebellar ataxia

(2020) Xiaofei Du et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Concatemers to re-investigate the role of α5 in α4β2 nicotinic receptors

(2020) Marie S. Prevost et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Remarkable effect of transdermal nicotine in children with CHRNA4-related autosomal dominant sleep-related hypermotor epilepsy

(2020) Kristine Lossius et al.   EPILEPSY & BEHAVIOR

Determining the correct stoichiometry of Kv2.1/Kv6.4 heterotetramers, functional in multiple stoichiometrical configurations

(2020) Lena Möller et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Heterologous expression of concatenated nicotinic ACh receptors: Pros and cons of subunit concatenation and recommendations for construct designs

(2020) Vivian Wan Yu Liao et al.   BRITISH JOURNAL OF PHARMACOLOGY

Cross-Talk between P2X and NMDA Receptors

(2020) Larry Rodriguez et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Functional modulation of the human voltage-gated sodium channel NaV1.8 by auxiliary β subunits

(2020) S. T. Nevin et al.   Channels

Nicotinic Receptors in Sleep-Related Hypermotor Epilepsy: Pathophysiology and Pharmacology

(2020) Andrea Becchetti et al.   Brain Sciences

Functional genomics of epilepsy-associated mutations in the GABAA receptor subunits reveal that one mutation impairs function and two are catastrophic

(2019) Nathan L. Absalom et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Photoswitchable Agonist for the Histamine H3 Receptor, a Prototypic Family A G-Protein-Coupled Receptor

(2019) Niels J. Hauwert et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Integration of Consortia Recommendations for Justification of Animal Use Within Current and Future Drug Development Paradigms

(2019) Helen Prior et al.   INTERNATIONAL JOURNAL OF TOXICOLOGY

Heterogeneous clinical and functional features of GRIN2D-related developmental and epileptic encephalopathy

(2019) Wenshu XiangWei et al.   BRAIN

De novo GRIN variants in NMDA receptor M2 channel pore‐forming loop are associated with neurological diseases

(2019) Jia Li et al.   HUMAN MUTATION

De novo variants in SLC12A6 cause sporadic early-onset progressive sensorimotor neuropathy

(2019) Joohyun Park et al.   JOURNAL OF MEDICAL GENETICS

Using Xenopus oocytes in neurological disease drug discovery

(2019) Steven L. Zeng et al.   Expert Opinion on Drug Discovery

The wonderland of neuronal nicotinic acetylcholine receptors

(2018) Daniel Bertrand et al.   BIOCHEMICAL PHARMACOLOGY

Pharmacogenomics of GPCR Drug Targets

(2018) Alexander S. Hauser et al.   CELL

Systematic variation of the benzoylhydrazine moiety of the GluN2A selective NMDA receptor antagonist TCN-201

(2018) Julian A. Schreiber et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

GRIN2A-related disorders: genotype and functional consequence predict phenotype

(2018) Vincent Strehlow et al.   BRAIN

Microtransplantation of human brain receptors into oocytes to tackle key questions in drug discovery

(2018) Ruud Zwart et al.   DRUG DISCOVERY TODAY

Development of Human Target Validation Classification that Predicts Future Clinical Efficacy

(2018) Cecilia Karlsson et al.   JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS

Lethal digenic mutations in the K+ channels Kir4.1 (KCNJ10) and SLACK (KCNT1) associated with severe-disabling seizures and neurodevelopmental delay

(2017) Sonia Hasan et al.   JOURNAL OF NEUROPHYSIOLOGY

Clinical and molecular characterization ofKCNT1-related severe early-onset epilepsy

(2017) Amy McTague et al.   NEUROLOGY

Molecular Mechanism of AMPA Receptor Modulation by TARP/Stargazin

(2017) Anat Ben-Yaacov et al.   NEURON

A De Novo Mutation in the Sodium-Activated Potassium Channel KCNT2 Alters Ion Selectivity and Causes Epileptic Encephalopathy

(2017) Sushmitha Gururaj et al.   Cell Reports

High and low GABA sensitivity α4β2δ GABAA receptors are expressed in Xenopus laevis oocytes with divergent stoichiometries

(2016) Leonny Y. Hartiadi et al.   BIOCHEMICAL PHARMACOLOGY

GABAA receptor-mediated neurotransmission: Not so simple after all

(2016) Frédéric Knoflach et al.   BIOCHEMICAL PHARMACOLOGY

Mutant SOD1 protein increases Nav1.3 channel excitability

(2016) Elif Kubat Öktem et al.   JOURNAL OF BIOLOGICAL PHYSICS

Reconstitution of synaptic Ion channels from rodent and human brain inXenopusoocytes: a biochemical and electrophysiological characterization

(2016) Francesca Mazzo et al.   JOURNAL OF NEUROCHEMISTRY

KCTD Hetero-oligomers Confer Unique Kinetic Properties on Hippocampal GABA B Receptor-Induced K + Currents

(2016) Thorsten Fritzius et al.   JOURNAL OF NEUROSCIENCE

All naturally occurring autoantibodies against the NMDA receptor subunit NR1 have pathogenic potential irrespective of epitope and immunoglobulin class

(2016) E Castillo-Gómez et al.   MOLECULAR PSYCHIATRY

Functional aspects of early brain development are preserved in tuberous sclerosis complex (TSC) epileptogenic lesions

(2016) Gabriele Ruffolo et al.   NEUROBIOLOGY OF DISEASE

Brain α7 Nicotinic Acetylcholine Receptor Assembly Requires NACHO

(2016) Shenyan Gu et al.   NEURON

Acetylcholine receptors from human muscle as pharmacological targets for ALS therapy

(2016) Eleonora Palma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Epilepsy-Related Slack Channel Mutants Lead to Channel Over-Activity by Two Different Mechanisms

(2016) Qiong-Yao Tang et al.   Cell Reports

When Quality Beats Quantity: Decision Theory, Drug Discovery, and the Reproducibility Crisis

(2016) Jack W. Scannell et al.   PLoS One

Disease progression and search for monogenic diabetes among children with new onset type 1 diabetes negative for ICA, GAD- and IA-2 Antibodies

(2016) Sven Pörksen et al.   BMC Endocrine Disorders

Pharmacological characterization of GABAB receptor subtypes assembled with auxiliary KCTD subunits

(2015) Mathieu Rajalu et al.   NEUROPHARMACOLOGY

A Quantitative Model of the GIRK1/2 Channel Reveals That Its Basal and Evoked Activities Are Controlled by Unequal Stoichiometry of Gα and Gβγ

(2015) Daniel Yakubovich et al.   PLoS Computational Biology

Discovery of Potent Positive Allosteric Modulators of the α3β2 Nicotinic Acetylcholine Receptor by a Chemical Space Walk in ChEMBL

(2014) Justus J. Bürgi et al.   ACS Chemical Neuroscience

KCNT1gain of function in 2 epilepsy phenotypes is reversed by quinidine

(2014) Carol J. Milligan et al.   ANNALS OF NEUROLOGY

Identification of a Point Mutation Impairing the Binding between Aquaporin-4 and Neuromyelitis Optica Autoantibodies

(2014) Francesco Pisani et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Lessons learned from the fate of AstraZeneca's drug pipeline: a five-dimensional framework

(2014) David Cook et al.   NATURE REVIEWS DRUG DISCOVERY

GRIN2Amutation and early-onset epileptic encephalopathy: personalized therapy with memantine

(2014) Tyler Mark Pierson et al.   Annals of Clinical and Translational Neurology

AMPA receptor/TARP stoichiometry visualized by single-molecule subunit counting

(2013) P. Hastie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure and activation of rhodopsin

(2012) X Edward Zhou et al.   ACTA PHARMACOLOGICA SINICA

Benzodiazepines Modulate GABAA Receptors by Regulating the Preactivation Step after GABA Binding

(2012) M. C. Gielen et al.   JOURNAL OF NEUROSCIENCE

De novo gain-of-function KCNT1 channel mutations cause malignant migrating partial seizures of infancy

(2012) Giulia Barcia et al.   NATURE GENETICS

Can the flow of medicines be improved? Fundamental pharmacokinetic and pharmacological principles toward improving Phase II survival

(2011) Paul Morgan et al.   DRUG DISCOVERY TODAY

Agonist-like autoantibodies against calcium channel in patients with dilated cardiomyopathy

(2011) Hua Xiao et al.   HEART AND VESSELS

Stargazing from a new vantage - TARP modulation of AMPA receptor pharmacology

(2011) Alexander C. Jackson et al.   JOURNAL OF PHYSIOLOGY-LONDON

Microtransplantation of acetylcholine receptors from normal or denervated rat skeletal muscles to frog oocytes

(2011) Annalisa Bernareggi et al.   JOURNAL OF PHYSIOLOGY-LONDON

Translational Control in Oocyte Development

(2011) J. D. Richter et al.   Cold Spring Harbor Perspectives in Biology

Principles of early drug discovery

(2010) JP Hughes et al.   BRITISH JOURNAL OF PHARMACOLOGY

Native GABAB receptors are heteromultimers with a family of auxiliary subunits

(2010) Jochen Schwenk et al.   NATURE

Ultrafast optogenetic control

(2010) Lisa A Gunaydin et al.   NATURE NEUROSCIENCE

Main Immunogenic Region Structure Promotes Binding of Conformation-Dependent Myasthenia Gravis Autoantibodies, Nicotinic Acetylcholine Receptor Conformation Maturation, and Agonist Sensitivity

(2009) J. Luo et al.   JOURNAL OF NEUROSCIENCE

A Functional Null Mutation of SCN1B in a Patient with Dravet Syndrome

(2009) G. A. Patino et al.   JOURNAL OF NEUROSCIENCE

Bi-stable neural state switches

(2009) André Berndt et al.   NATURE NEUROSCIENCE

Microtransplantation of ligand-gated receptor-channels from fresh or frozen nervous tissue into Xenopus oocytes: A potent tool for expanding functional information

(2009) F. Eusebi et al.   PROGRESS IN NEUROBIOLOGY

Non-Agonist-Binding Subunit Interfaces Confer Distinct Functional Signatures to the Alternate Stoichiometries of the  4 2 Nicotinic Receptor: An  4- 4 Interface Is Required for Zn2+ Potentiation

(2008) M. Moroni et al.   JOURNAL OF NEUROSCIENCE