Article
Medicine, Research & Experimental
Catielen Paula Pavi, Isabella Dai Pra, Rafael Dorighello Cadamuro, Isamu Kanzaki, Jhuly Wellen Ferreira Lacerda, Louis Pergaud Sandjo, Roberto Messias Bezerra, Jorge Federico Orellana Segovia, Gislaine Fongaro, Izabella Thais Silva
Summary: This study evaluated the activity of seventeen extracts derived from eight Amazonian medicinal plants against herpes simplex virus and chikungunya virus. Four extracts exhibited significant activity against both viruses, including the drug-resistant strains. Virucidal tests showed a higher level of virucidal activity compared to antiviral activity.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Alexander Michels, Annika M. Frank, Dorothee M. Guenther, Mehryad Mataei, Kathleen Borner, Dirk Grimm, Jessica Hartmann, Christian J. Buchholz
Summary: This study successfully improved gene delivery efficiency to CD8+ mouse lymphocytes using mCD8-LV, based on DARPin binding to murine CD8. The results suggest a potential application of DARPins in AAV vectors for targeted gene therapy.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Virology
Anita Felicitas Meier, Kurt Tobler, Kevin Michaelsen, Bernd Vogt, Els Henckaerts, Cornel Fraefel
Summary: The study shows that HSV-1 modulates AAV genome end recombination, potentially through mechanisms involving the ICP8 protein. Despite changes in the type of end recombination, no significant alteration in circular AAV genome abundance was observed.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Tajanna Stinn, Steve Kuntz, Dana Varon, Meei-Li Huang, Stacy Selke, Samuel Njikan, Emily S. Ford, Joan Dragavon, Robert W. Coombs, Christine Johnston, Marta E. Bull
Summary: This study suggests that subclinical HSV shedding in HIV/HSV-coinfected women during ART may sustain HIV tissue reservoirs through antigen exposure or HIV replication. Frequent subclinical HSV DNA shedding was associated with increased HIV DNA tissue concentrations and increased divergence from ancestral sequences, indicating a potential role of immune responses to common coinfections in sustaining HIV tissue reservoirs during suppressive ART.
JOURNAL OF VIROLOGY
(2021)
Article
Pharmacology & Pharmacy
David I. Bernstein, Nancy M. Sawtell, Fernando J. Bravo, David A. Dixon, Christian Gege, Gerald Kleymann
Summary: The study investigates a treatment method for latent neuronal herpes simplex virus infections. The use of the helicase-primase inhibitor (HPI) IM-250 reduces the reactivation frequency significantly.
ANTIVIRAL RESEARCH
(2023)
Article
Pathology
Melissa Krystel-Whittemore, May P. Chan, Sara C. Shalin, Kenan J. Sauder, Amy Hudson, Ruth K. Foreman, Mai P. Hoang, Jeoffry B. Brennick, Shaofeng Yan, Rosalynn M. Nazarian
Summary: This study presents the first known report of herpes virus infecting deep stromal cells of the dermis, highlighting the importance of considering cutaneous stromal herpes in patients with atypical clinical lesions, especially when immunocompromised. Establishing the correct diagnosis is crucial for initiating therapy.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Pharmacology & Pharmacy
Lyudmila Alimbarova, Anna Egorova, Olga Riabova, Natalia Monakhova, Vadim Makarov
Summary: Herpes simplex keratitis is a significant cause of blindness worldwide. The current antiviral treatment may face resistance issues, highlighting the urgent need for backup medications. The small molecule PDSTP has shown activity against herpes simplex type 1 strains in vitro and demonstrated preclinical efficacy in a rabbit model of herpes simplex epithelial keratitis. These findings provide new opportunities for the development of antiherpetic drugs with a novel mechanism of action.
ANTIVIRAL RESEARCH
(2022)
Article
Dermatology
Deepthi Konda, Laxmisha Chandrashekar, Rahul Dhodapkar, Rajesh Nachiappa Ganesh, Devinder Mohan Thappa
Summary: This study characterized the clinical markers of herpes simplex virus (HSV) infection among patients with pemphigus vulgaris. It was found that male sex, presence of fissures, hemorrhagic crusts, erosions with angulated margins, linear erosions, and raised erythrocyte sedimentation rate were significantly associated with HSV infection. Hemorrhagic crusts and linear erosions were identified as independent predictors of HSV infection.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2023)
Article
Immunology
Malgorzata Krzyzowska, Anders Jarneborn, Karolina Thorn, Kristina Eriksson, Tao Jin
Summary: This study provides evidence that treatment with tofacitinib may increase the risk of disease aggravation and severe encephalitis in primary herpes simplex infection by impairing antiviral response induced by monocytes and microglia.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Cooper K. Hayes, Christopher K. Villota, Fiona B. McEnany, Stacey Ceron, Sita Awasthi, Moriah L. Szpara, Harvey M. Friedman, David A. Leib, Richard Longnecker, Matthew D. Weitzman, Lisa N. Akhtar
Summary: Clinical HSV-2 isolates collected from neonates with encephalitis are more neurovirulent in human neuronal cell culture and murine models as compared to isolates from neonates with skin-limited disease, suggesting that viral factors contribute to neurologic outcome following human neonatal infection.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Virology
Bangxing Hong, Upasana Sahu, Matthew P. Mullarkey, Balveen Kaur
Summary: Oncolytic herpes simplex virus (oHSV) shows promise as a treatment for solid tumors, but there are still barriers that limit its efficacy. This review focuses on the essential viral genes that allow for virus replication and spread, with the aim of enhancing oHSV infection and replication among tumor cells.
Article
Microbiology
Kevin Danastas, Gerry Guo, Jessica Merjane, Nathan Hong, Ava Larsen, Monica Miranda-Saksena, Anthony L. Cunningham
Summary: This study explores the effects of interferons (IFNs) on herpes simplex virus-1 (HSV-1) and reveals that IFNs have the potential to block virus release from nerve endings, thereby preventing transmission into the skin. The study also highlights the potential wider antiviral effects of IFN-γ in neurons, suggesting its role in HSV-1 reactivation. These findings identify new targets for the development of immunotherapies to impede HSV-1 spread from nerves to the skin.
Article
Virology
Mashidur Rana, Mizumi Setia, Pratima K. Suvas, Anish Chakraborty, Susmit Suvas
Summary: Reactive oxygen species (ROS) play a crucial role in herpes stromal keratitis (HSK), with neutrophils being the primary cells responsible for ROS generation. NOX2 is involved in ROS production in neutrophils. However, manipulating ROS levels in neutrophils may help alleviate the severity of HSK, and NOX2 is not the main contributor to ROS generation in neutrophils.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Mette Ratzer Freytag, Sofie Eg Jorgensen, Michelle Molgaard Thomsen, Ali Al-Mousawi, Alon Schneider Hait, David Olagnier, Jakob T. Bay, Marie Helleberg, Trine H. Mogensen
Summary: This study describes a 19-year-old woman with systemic HSV-1 infection and HLH, as well as a fatal course of neonatal herpesvirus infection postpartum. Investigation showed impaired antiviral responses in the mother's cells, potentially caused by variants in CASP8 or other noncoding regions of the genome.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Chemistry, Multidisciplinary
Scott M. Stagg, Craig Yoshioka, Omar Davulcu, Michael S. Chapman
Summary: Adeno-associated virus (AAV) is a leading delivery vector in gene therapy treatments. Understanding the structure and molecular interactions of AAV with human hosts is of great interest. Electron microscopic approaches have been used to visualize the virus and its complexes, leading to important findings and new insights.
Article
Medicine, Research & Experimental
Gloriane Schnabolk, Nathaniel Parsons, Elisabeth Obert, Balasubramaniam Annamalai, Cecile Nasarre, Stephen Tomlinson, Alfred S. Lewin, Barbel Rohrer
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2018)
Article
Ophthalmology
Chulbul M. Ahmed, Michael T. Massengill, Emily E. Brown, Cristhian J. Ildefonso, Howard M. Johnson, Alfred S. Lewin
EXPERIMENTAL EYE RESEARCH
(2018)
Editorial Material
Oncology
Zachary L. Watson, Benjamin G. Bitler
Article
Oncology
Lindsay J. Wheeler, Zachary L. Watson, Lubna Qamar, Tomomi M. Yamamoto, Miriam D. Post, Amber A. Berning, Monique A. Spillman, Kian Behbakht, Benjamin G. Bitler
Article
Biochemistry & Molecular Biology
Rekha R. Garg, Cody B. Jackson, Masmudur M. Rahman, Amir R. Khan, Alfred S. Lewin, Grant McFadden
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Emily E. Brown, Alexander J. DeWeerd, Cristhian J. Ildefonso, Alfred S. Lewin, John D. Ash
Article
Oncology
Pyoung Hwa Park, Tomomi M. Yamamoto, Hua Li, Allen L. Alcivar, Bing Xia, Yifan Wang, Andrea J. Bernhardy, Kristen M. Turner, Andrew V. Kossenkov, Zachary L. Watson, Kian Behbakht, Silvia Casadei, Elizabeth M. Swisher, Paul S. Mischel, Neil Johnson, Benjamin G. Bitler
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Medicine, General & Internal
Raela B. Ridley, Brianna M. Young, Jieun Lee, Erin Walsh, Chulbul M. Ahmed, Alfred S. Lewin, Cristhian J. Ildefonso
JOURNAL OF CLINICAL MEDICINE
(2019)
Article
Multidisciplinary Sciences
Chulbul M. Ahmed, Cristhian J. Ildefonso, Howard M. Johnson, Alfred S. Lewin
Article
Oncology
Brandon T. Sawyer, Lubna Qamar, Tomomi M. Yamamoto, Alexandra McMellen, Zachary L. Watson, Jennifer K. Richer, Kian Behbakht, Isabel R. Schlaepfer, Benjamin G. Bitler
MOLECULAR CANCER RESEARCH
(2020)
Article
Ophthalmology
Neil F. Ash, Michael T. Massengill, Lindsey Harmer, Ahmed Jafri, Alfred S. Lewin
Summary: Microglia, immune cells of the central nervous system, respond to injury with phenotypic changes and migration. An automated segmentation routine is proposed for efficient processing and analysis of retinal microglia.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Manas R. Biswal, Zhaoyao Wang, Ryan J. Paulson, Rukshana R. Uddin, Yao Tong, Ping Zhu, Hong Li, Alfred S. Lewin
Summary: Modified form of EPO, EPO-R76E, attenuates erythropoietic activity but effectively inhibits apoptosis, oxidative stress, and inflammation in retinal degeneration. Long-term sustained delivery of EPO-R76E using AAV can prevent RPE oxidative stress-induced retinal degeneration, potentially providing a tool for preventing Age-Related Macular Degeneration.
Article
Medicine, Research & Experimental
Alfred S. S. Lewin, W. Clay Smith
Summary: Mutations in the RHO gene are a significant cause of autosomal-dominant retinitis pigmentosa. There are two clinical classes of patients - those with early onset and those with slowly progressive disease. Gene therapy can target the mutant gene at the DNA or RNA level, while other therapies aim to preserve photoreceptor viability.
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE
(2022)
Article
Neurosciences
Chulbul M. Ahmeda, Michael T. Massengill, Cristhian J. Ildefonso, Archana Jalligampala, Ping Zhu, Hung Li, Anil P. Patel, Maureen A. McCall, Alfred S. Lewin
Summary: This study found that AAV-RHO820-shRNA820 can slow the loss of photoreceptor cells and preserve retinal function in P23H RHO transgenic mice. Moreover, the untreated contralateral eyes also showed the same protective effect. This suggests that RNA replacement therapy may provide clinical benefit to both eyes of adRP patients.
Article
Biochemistry & Molecular Biology
Benjamin G. G. Bitler, Courtney A. A. Bailey, Tomomi M. M. Yamamoto, Alexandra McMellen, Hyunmin Kim, Zachary L. L. Watson
Summary: PARP inhibitors kill cancer cells by stalling DNA replication and preventing DNA repair. Resistance to PARP inhibitors is becoming a clinical problem in the treatment of high grade serous ovarian carcinoma (HGSOC). This study investigates the expression and activities of histone acetyltransferases (HATs) and epigenetic readers in PARP inhibitor-resistant HGSOC, and assesses the therapeutic potential of targeting H3K14ac or associated proteins.
MOLECULAR CARCINOGENESIS
(2023)