Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag

A flexible split prime editor using truncated reverse transcriptase improves dual-AAV delivery in mouse liver

(2022) Chunwei Zheng et al.   MOLECULAR THERAPY

A split prime editor with untethered reverse transcriptase and circular RNA template

(2022) Bin Liu et al.   NATURE BIOTECHNOLOGY

Genomic and Transcriptomic Analyses of Prime Editing Guide RNA–Independent Off-Target Effects by Prime Editors

(2022) Runze Gao et al.   CRISPR Journal

Engineered CRISPR prime editors with compact, untethered reverse transcriptases

(2022) Julian Grünewald et al.   NATURE BIOTECHNOLOGY

Human genetic diversity alters off-target outcomes of therapeutic gene editing

(2022) Samuele Cancellieri et al.   NATURE GENETICS

PEAC-seq adopts Prime Editor to detect CRISPR off-target and DNA translocation

(2022) Zhenxing Yu et al.   Nature Communications

CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells

(2021) Karl Petri et al.   NATURE BIOTECHNOLOGY

CRISPR therapies march into clinic, but genotoxicity concerns linger

(2021) Cormac Sheridan   NATURE BIOTECHNOLOGY

Prime editing progress

(2021) Lei Tang   NATURE METHODS

Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases

(2021) Hyewon Jang et al.   Nature Biomedical Engineering

Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids

(2021) Maarten H Geurts et al.   Life Science Alliance

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

(2021) Peter J. Chen et al.   CELL

Engineered pegRNAs improve prime editing efficiency

(2021) James W. Nelson et al.   NATURE BIOTECHNOLOGY

CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery by AAV Vectors

(2020) Dan Wang et al.   CELL

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR–Cas gene editing

(2020) Qiang Cheng et al.   Nature Nanotechnology

Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

(2020) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant System

(2020) Jenny Shapiro et al.   Molecular Therapy-Methods & Clinical Development

Predicting the efficiency of prime editing guide RNAs in human cells

(2020) Hui Kwon Kim et al.   NATURE BIOTECHNOLOGY

Unbiased investigation of specificities of prime editing systems in human cells

(2020) Do Yon Kim et al.   NUCLEIC ACIDS RESEARCH

Tools for experimental and computational analyses of off-target editing by programmable nucleases

(2020) X. Robert Bao et al.   Nature Protocols

Enhanced Cas12a editing in mammalian cells and zebrafish

(2019) Pengpeng Liu et al.   NUCLEIC ACIDS RESEARCH

CRISPResso2 provides accurate and rapid genome editing sequence analysis

(2019) Kendell Clement et al.   NATURE BIOTECHNOLOGY

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

UDiTaS™, a genome editing detection method for indels and genome rearrangements

(2018) Georgia Giannoukos et al.   BMC GENOMICS

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

In vivo CRISPR editing with no detectable genome-wide off-target mutations

(2018) Pinar Akcakaya et al.   NATURE

Isogenic cell models of cystic fibrosis-causing variants in natively expressing pulmonary epithelial cells

(2018) Hillary C. Valley et al.   Journal of Cystic Fibrosis

RettBASE: Rett syndrome database update

(2017) Rahul Krishnaraj et al.   HUMAN MUTATION

Mapping the genomic landscape of CRISPR–Cas9 cleavage

(2017) Peter Cameron et al.   NATURE METHODS

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases

(2014) Richard L Frock et al.   NATURE BIOTECHNOLOGY

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH