Probing Ligand Binding Sites on Large Proteins by Nuclear Magnetic Resonance Spectroscopy of Genetically Encoded Non-Canonical Amino Acids

Genetic Encoding of Cyanopyridylalanine for In‐Cell Protein Macrocyclization by the Nitrile–Aminothiol Click Reaction

(2022) Elwy H. Abdelkader et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Incorporation of 7-Fluoro-L-tryptophan into Proteins by Genetic Encoding to Monitor Ligand Binding by 19F NMR Spectroscopy

(2022) Haocheng Qianzhu et al.   ACS Sensors

Main protease mutants of SARS-CoV-2 variants remain susceptible to nirmatrelvir

(2022) Sven Ullrich et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling

(2022) Haoyi Ge et al.   MOLECULES

Predicting Antiviral Resistance Mutations in SARS-CoV-2 Main Protease with Computational and Experimental Screening

(2022) Vishnu M. Sasi et al.   BIOCHEMISTRY

Genetic Encoding of N6-(((Trimethylsilyl)methoxy)carbonyl)-l-lysine for NMR Studies of Protein–Protein and Protein–Ligand Interactions

(2021) Elwy H. Abdelkader et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease

(2021) Sebastian Günther et al.   SCIENCE

The dimer-monomer equilibrium of SARS-CoV-2 main protease is affected by small molecule inhibitors

(2021) Lucia Silvestrini et al.   Scientific Reports

Advances in the study of GPCRs by 19F NMR

(2021) Louis-Philippe Picard et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Through-Space Scalar 19F–19F Couplings between Fluorinated Noncanonical Amino Acids for the Detection of Specific Contacts in Proteins

(2021) Henry W. Orton et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Genetically Encoded F-19 NMR Probe Reveals the Allosteric Modulation Mechanism of Cannabinoid Receptor 1

(2021) Xiaoyan Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structure of Mpro from SARS-CoV-2 and discovery of its inhibitors

(2020) Zhenming Jin et al.   NATURE

Deuteration of nonexchangeable protons on proteins affects their thermal stability, side‐chain dynamics, and hydrophobicity

(2020) Parker J. Nichols et al.   PROTEIN SCIENCE

Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors

(2020) Linlin Zhang et al.   SCIENCE

Allosteric Inhibition of the SARS‐CoV‐2 Main Protease: Insights from Mass Spectrometry Based Assays

(2020) Tarick J. El‐Baba et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetic Encoding of para-Pentafluorosulfanyl Phenylalanine: A Highly Hydrophobic and Strongly Electronegative Group for Stable Protein Interactions

(2020) Haocheng Qianzhu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mutant T4 DNA polymerase for easy cloning and mutagenesis

(2019) Ruhu Qi et al.   PLoS One

Exposing the Origins of Irreproducibility in Fluorine NMR Spectroscopy

(2018) Carl Philipp Rosenau et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A genetically encoded 19F NMR probe for lysine acetylation

(2018) Feng Zhang et al.   CHEMICAL COMMUNICATIONS

NMR studies of ligand binding

(2018) Christoph Nitsche et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Genetically encoded amino acids with tert-butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy

(2017) Choy Theng Loh et al.   JOURNAL OF BIOMOLECULAR NMR

O-tert-Butyltyrosine, an NMR Tag for High-Molecular-Weight Systems and Measurements of Submicromolar Ligand Binding Affinities

(2015) Wan-Na Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon

(2015) Takahito Mukai et al.   Scientific Reports

Fluorine-19 NMR of integral membrane proteins illustrated with studies of GPCRs

(2013) Tatiana Didenko et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Peptide aldehyde inhibitors challenge the substrate specificity of the SARS-coronavirus main protease

(2011) Lili Zhu et al.   ANTIVIRAL RESEARCH

Deuteration of Escherichia coli Enzyme INtr alters its stability

(2010) Grzegorz Piszczek et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS