E. coli “Stablelabel” S30 lysate for optimized cell-free NMR sample preparation

Biochemical Characterization of Cell-free Synthesized Human β1 Adrenergic Receptor Cotranslationally Inserted into Nanodiscs

(2022) Zoe Köck et al.   JOURNAL OF MOLECULAR BIOLOGY

Cotranslational assembly of membrane protein/nanoparticles in cell-free systems

(2022) Roman Levin et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Backbone and methyl resonances assignment of the 87 kDa prefoldin from Pyrococcus horikoshii

(2021) Ricarda Törner et al.   Biomolecular NMR Assignments

Rapid RNase inhibitor production to enable low‐cost, on‐demand cell‐free protein synthesis biosensor use in human body fluids

(2021) Mehran Soltani et al.   BIOTECHNOLOGY AND BIOENGINEERING

A high-resolution description of β1-adrenergic receptor functional dynamics and allosteric coupling from backbone NMR

(2020) Anne Grahl et al.   Nature Communications

Enabling NMR studies of high molecular weight systems without the need for deuteration: The XL‐ALSOFAST experiment with delayed decoupling

(2020) Philip Rößler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Conformational Equilibrium of the Neuropeptide Y2 Receptor in Bilayer Membranes

(2020) Ulrike Krug et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

GPCR Activation States Induced by Nanobodies and Mini-G Proteins Compared by NMR Spectroscopy

(2020) Philip Rößler et al.   MOLECULES

A simple, robust, and low-cost method to produce the PURE cell-free system.

(2019) Barbora Lavickova et al.   ACS Synthetic Biology

Towards complete polypeptide backbone NH assignment via combinatorial labeling

(2019) Frank Löhr et al.   JOURNAL OF MAGNETIC RESONANCE

Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems

(2019) Stefan Schütz et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Precursor-Based Selective Methyl Labeling of Cell-Free Synthesized Proteins

(2018) Mariya Lazarova et al.   ACS Chemical Biology

The E. coli S30 lysate proteome: A prototype for cell-free protein production

(2018) Daniel Foshag et al.   New Biotechnology

Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis

(2018) Beate Hoffmann et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR

(2017) Franz Hagn et al.   Nature Protocols

Nanodiscs and solution NMR: preparation, application and challenges

(2017) Robbins Puthenveetil et al.   Nanotechnology Reviews

Backbone NMR reveals allosteric signal transduction networks in the β1-adrenergic receptor

(2016) Shin Isogai et al.   NATURE

CRMAGE: CRISPR Optimized MAGE Recombineering

(2016) Carlotta Ronda et al.   Scientific Reports

The PURE system for the cell-free synthesis of membrane proteins

(2015) Yutetsu Kuruma et al.   Nature Protocols

Synthesis of Metabolic Amino acid Precursors: Tools for Selective Isotope Labeling in Cell-Based Protein Overexpression

(2015) Roman Lichtenecker et al.   SYNLETT

Scrambling free combinatorial labeling of alanine-β, isoleucine-δ1, leucine-proS and valine-proS methyl groups for the detection of long range NOEs

(2014) Rime Kerfah et al.   JOURNAL OF BIOMOLECULAR NMR

Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression

(2014) Rasmus Linser et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Molecular signatures of G-protein-coupled receptors

(2013) A. J. Venkatakrishnan et al.   NATURE

FRUIT, a Scar-Free System for Targeted Chromosomal Mutagenesis, Epitope Tagging, and Promoter Replacement in Escherichia coli and Salmonella enterica

(2012) Anne M. Stringer et al.   PLoS One

A practical method for cell-free protein synthesis to avoid stable isotope scrambling and dilution

(2011) Jun Yokoyama et al.   ANALYTICAL BIOCHEMISTRY

Solution NMR Structure of Proteorhodopsin

(2011) Sina Reckel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Hydrogen exchange during cell-free incorporation of deuterated amino acids and an approach to its inhibition

(2011) Marco Tonelli et al.   JOURNAL OF BIOMOLECULAR NMR

Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O

(2011) Xun-Cheng Su et al.   JOURNAL OF BIOMOLECULAR NMR

A rapid and robust method for selective isotope labeling of proteins

(2011) Myat T. Lin et al.   METHODS

Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins

(2010) Pierre Gans et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Longitudinal-Relaxation-Enhanced NMR Experiments for the Study of Nucleic Acids in Solution

(2009) Jonathan Farjon et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY