Review
Biochemistry & Molecular Biology
Dieuwertje Augustijn, Huub J. M. de Groot, A. Alia
Summary: Metabolomics is a method used to simplify the complexity of plants and understand the pathways of plant phenotypes. HR-MAS NMR can eliminate the extraction step of metabolites from samples, saving time and costs. HR-MAS NMR has important applications in plant metabolomics.
Article
Biochemistry & Molecular Biology
Carolina da Silva Canielles Caprara, Tatiane Ksyvickas Mathias, Maria de Fatima C. Santos, Marcelo G. M. D'Oca, Caroline Da R. M. D'Oca, Fabio Roselet, Paulo Cesar Abreu, Daniela Fernandes Ramos
Summary: This study aimed to characterize the main metabolites of three species of microalgae using H-1 HR-MAS NMR. Although the three species were found in the same ecosystem and had a high production of lipid compounds, significant differences were identified in the production of specialized metabolites. These unique properties make these compounds potential leaders in the development of new bioactive compounds for One Health and alternatives for aquaculture.
Article
Biochemistry & Molecular Biology
Martina Vermathen, Hendrik von Tengg-Kobligk, Martin Nils Hungerbuehler, Peter Vermathen, Nico Ruprecht
Summary: This study used high-resolution magic angle spinning (HR-MAS) NMR to analyze the metabolic profiles of non-small cell lung cancer (NSCLC) cells and their adaptations to cisplatin (cisPt) resistance. The findings suggest that characteristic metabolic patterns for cisPt resistance may serve as potential biomarkers in cancer medicine.
Article
Food Science & Technology
Jie Zhao, Wenwen Liu, Yan Chen, Xin Zhang, Xu Wang, Fuhua Wang, Yongzhong Qian, Jing Qiu
Summary: This study identified potential markers for tea authenticity assessment using non-targeted metabolomics in unique oolong tea cultivars. A classification model based on these markers was developed and successfully discriminated the oolong tea samples. The combination of non-targeted metabolomics and chemometric methods can provide high resolution markers for tea authenticity assessment.
Article
Biochemistry & Molecular Biology
Yushi Zou, Chen Tang, Xinyu Yang, Tianyang Guo, Huanlu Song
Summary: A study was conducted on the flavor characteristics of different grades of baked green tea. It was found that D-limonene and linalool were identified as odor markers for Grade A tea, while galloylglucose, digalloylglucose, trigalloyglucose, strictinin, and gallic acid were identified as taste markers for Grade A tea. This research reveals the substances responsible for the odor and taste markers of high-grade baked green tea.
Article
Plant Sciences
Ming-zhi Zhu, Fang Zhou, Li-sha Ran, Yi-long Li, Bin Tan, Kun-bo Wang, Jian-an Huang, Zhong-hua Liu
Summary: Purple-leaf tea cultivars are known for their specific chemical composition that greatly influences tea bioactivity and plant resistance. Studies have shown that the high anthocyanin and low chlorophyll content contribute to the purple-leaf phenotype, with key genes such as 4CL, ANS, UFGT, and HEME playing crucial roles in the formation of purple leaves. These findings provide insights into the mechanism of purple-leaf formation in tea cultivars.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Applied
Valeria Vanoli, Sara Delleani, Mose Casalegno, Fabio Pizzetti, Pooyan Makvandi, Havard Haugen, Andrea Mele, Filippo Rossi, Franca Castiglione
Summary: Hydrogels based on hyaluronic acid and agarose-carbomer have attracted attention as drug delivery systems. The structure of the polymer network greatly affects the release capability of these materials. Using HR-MAS NMR spectroscopy, this study investigated the motion of drugs within the hydrogels and found evidence of superdiffusive motion, which may be responsible for the faster release of sodium salicylate.
CARBOHYDRATE POLYMERS
(2023)
Article
Agriculture, Multidisciplinary
Ting Gao, Bing-hao Hou, Shu-xian Shao, Meng-ting Xu, Yu-cheng Zheng, Shan Jin, Peng-jie Wang, Nai-xing Ye
Summary: In this study, we conducted transcriptomic and metabolomic analyses on young shoots of seven tea cultivars and identified key regulatory transcription factors (TFs) for characteristic metabolites. These findings provide a theoretical reference for tea cultivar breeding and suitability.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Hind Emad Fadoul, Felix Juan Martinez Rivas, Kerstin Neumann, Salma Balazadeh, Alisdair R. Fernie, Saleh Alseekh
Summary: This study evaluated the effects of simulated long-term water deficit stress on two contrasting African wheat cultivars. Results showed that Wadielniel exhibited higher tolerance to drought stress with significant changes in metabolites compared to Condor. Metabolites such as amino acids, proline, organic acids, and lipid classes were identified as potentially playing a role in drought stress regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Valtteri Virtanen, Susanna Raikkonen, Elina Puljula, Maarit Karonen
Summary: The study revealed that ellagitannins interacting with lipids share clear structural similarities, with all interacting ellagitannins possessing glucopyranose cores. The most important structural feature affecting the interaction appeared to be the structural flexibility of the ellagitannin, as even dimeric and trimeric ellagitannins could penetrate lipid bilayers if their structures were flexible.
Article
Food Science & Technology
Litao Sun, Xue Dong, Yonglin Ren, Manjree Agarwal, Alexander Ren, Zhaotang Ding
Summary: This study investigated the impact of brewing time on the volatile features of green tea infusion using HS-SPME and DI-SPME techniques. It was found that 2-3 minutes was the optimal brewing time. The key aromatic contributors in the tea infusion tended to equilibrate with the volatile compounds in the headspace over the infusion.
Article
Food Science & Technology
Hanchen Zhou, Yaqin Liu, Jihong Yang, Hui Wang, Yong Ding, Pandeng Lei
Summary: This study analyzes the aroma compounds of Taiping Houkui green tea and suggests that the unique aroma of this tea depends on both the tea plant variety and the manufacturing process.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Yoshinori Fujimura, Mototsugu Watanabe, Tomomi Morikawa-Ichinose, Konatsu Fujino, Mao Yamamoto, Seita Nishioka, Chihiro Inoue, Fumiyo Ogawa, Madoka Yonekura, Akari Nakasone, Motofumi Kumazoe, Hirofumi Tachibana
Summary: This study examined the DPP-IV inhibitory activity of leaf extracts from different Japanese green tea cultivars using liquid chromatography-mass spectrometry. It revealed differences in inhibitory rates among tea extracts and identified new compounds as DPP-IV inhibitors. The study also found that adding these compounds in combination can enhance the bioactivity of tea extract.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Horticulture
Chang Ha Park, Wooyeal Ki, Nam Su Kim, Soo-Yun Park, Jae Kwang Kim, Sang Un Park
Summary: This study comprehensively analyzed primary and secondary metabolites in the roots of white and green radishes. The levels of amino acids and phenolic acids were higher in green radishes, while slightly higher levels of glucosinolates were observed in white radishes. Glutamic acid was positively correlated with other amino acids and chlorophylls, while phenylalanine was positively correlated with phenolic compounds. Strong positive correlations were also observed between carbohydrates and phenolics.
Article
Biochemistry & Molecular Biology
Maha Montaser, Asmaa T. Ali, Ahmed M. Sayed, Usama Ramadan Abdelmohsen, Ehab W. Zidan, Raha Orfali, Mostafa E. Rateb, Mohamed A. Zaki, Hossam M. Hassan, Rabab Mohammed, Mohamed S. Hifnawy
Summary: This study investigated the metabolite composition and antioxidant activity of citrus honey, marjoram honey, and clover honey using H-1-NMR technique. The results showed that citrus honey and marjoram honey exhibited higher antioxidant activity compared to clover honey.
Article
Biochemical Research Methods
Camila de Souza Araujo, Ana Paula de Oliveira, Alan Diego da Conceicao Santos, Amanda Leite Guimaraes, Naiane Darklei dos Santos Silva, Mario Adriano Avila Queiroz, Edigenia Cavalcante da Cruz Araujo, Jackson Roberto Guedes da Silva Almeida
PHYTOCHEMICAL ANALYSIS
(2019)
Article
Chemistry, Applied
Ana Edileia Barbosa Pereira Leal, Ana Paula de Oliveira, Raira Feitosa dos Santos, Juliana Mikaelly Dias Soares, Erica Martins de Lavor, Michelle Cruz Pontes, Julianeli Tolentino de Lima, Alan Diego da Conceicao Santos, Jose Carlos Tomaz, Gibson Gomes de Oliveira, Fausto Carnevale Neto, Norberto Peporine Lopes, Larissa Araujo Rolim, Jackson Roberto Guedes da Silva Almeida
NATURAL PRODUCT RESEARCH
(2020)
Article
Multidisciplinary Sciences
Ana Paula de Oliveira, Ivanildo Viana Borges, Emanuella Chiara Valenca Pereira, Thiala Alves Feitosa, Raira Feitosa dos Santos, Raimundo Goncalves de Oliveira-Junior, Larissa Araujo Rolim, Lucas Gustavo Ferreira Cordeiro Viana, Luciano Augusto de Araujo Ribeiro, Alan Diego da Conceicao Santos, Pedro Jose Rolim-Neto, Jackson Roberto Guedes da Silva Almeida
Article
Plant Sciences
L. L. S. Silva, I. M. S. Araujo, P. M. O. Costa, A. P. S. Oliveira, A. P. Oliveira, A. D. C. Santos, L. M. Dutra, J. R. G. S. Almeida, L. C. B. B. Coelho, C. M. Souza-Motta, T. H. Napoleao, P. M. G. Paiva
SOUTH AFRICAN JOURNAL OF BOTANY
(2020)
Review
Biochemistry & Molecular Biology
Noelly Bastos Cavalcante, Alan Diego da Conceicao Santos, Jackson Roberto Guedes da Silva Almeida
CHEMICO-BIOLOGICAL INTERACTIONS
(2020)
Article
Chemistry, Physical
Rafaely N. Lima, Alan Diego C. Santos, Adauto S. Ribeiro, Lucio Cardozo-Filho, Lisiane S. Freitas, Andersson Barison, Emmanoel Costa, Pericles B. Alves
JOURNAL OF SUPERCRITICAL FLUIDS
(2020)
Article
Chemistry, Analytical
Livia Macedo Dutra, Alan Diego da Conceicao Santos, Allan Vinicius Felix Lourenco, Noemi Nagata, Gustavo Heiden, Francinete Ramos Campos, Andersson Barison
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Chemistry, Multidisciplinary
Pedro Lucas L. Souza, Andrezza S. Ramos, Alan D. C. Dos Santos, Lucia S. Boeira, Jaqueline de A. Bezerra, Marcos B. Machado
Summary: The objective of this study was to optimize the production process of fermented alcoholic beverages of araca-boi to increase antioxidant capacity. The results showed that temperature was the most relevant factor to the antioxidant response, while the sensory attributes were determined by the interaction of all factors investigated.
Article
Food Science & Technology
Livia Macedo Dutra, Pedro Henrique Vieira Teles, Alan Diego da Conceicao Santos, Natoniel Franklin de Melo, Noemi Nagata, Jackson Roberto Guedes da Silva Almeida
Summary: In this research, 1H NMR-based metabolic profiling was used to analyze extracts obtained from different wild Passiflora species and genetic varieties. Fourteen metabolites, including fatty acids, carbohydrates, saponins, alkaloids, and C-glycosidic flavones, were identified. Principal components analysis (PCA) allowed discrimination of Passiflora extracts and revealed the relevance of specific metabolites in differentiating between species and genetic varieties.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Multidisciplinary
Henrique M. M. Cezar, Joao Vito B. de Freitas, Alan Diego C. Santos, Nagila M. P. S. Ricardo, Nilce V. V. Gramosa
Summary: Diffusion-ordered spectroscopy (DOSY) is an NMR method used to separate components in mixtures based on their diffusion coefficients. In this study, the diffusion behavior of essential oil components and eugenol derivatives was investigated using DOSY with the surfactant Brij 78 as a matrix. The results showed that the choice of solvent and surfactant concentration significantly affected the resolution of the spectra. The best separation was achieved using a mixture of DMSO-d(6):D2O (1:1) with 40 mM Brij 78.
Article
Chemistry, Multidisciplinary
Ursula A. Ribeiro, Leonardo C. Queiroz, Agide G. Marassi, Andre S. Carvalho, Gabriel A. Barros, Daniel M. Consalter, Jaqueline A. Bezerra, Alan D. C. Santos, Luiz Alberto Colnago, Marcos B. Machado
Summary: A TD-NMR method was developed and validated in this study to assess the oil content of Brazil nuts, with results showing its reliability and robustness, indicating potential adoption as an industrial reference method for monitoring Brazil nuts oil content.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Applied
Alessandra Gomes Marques Pacheco, Alexsandro Branco, Celso Amorim Camara, Tania Maria Sarmento Silva, Telma Maria Guedes Silva, Ana Paula de Oliveira, Alan Diego da Conceicao Santos, Livia Macedo Dutra, Larissa Araujo Rolim, Gibson Gomes de Oliveira, Jacqueline Nakau Mendonca, Norberto Peporine Lopes, Jackson Roberto Guedes da Silva Almeida
Summary: Through HPLC-DAD and LC-MSn analysis, this study revealed that the native tree Hymenaea martiana in Brazil, known as "jatobá," is a good source of flavonoids, especially with a high concentration of astilbin. These compounds could be important chemotaxonomic markers for the genus Hymenaea.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
C. S. C. Alves, A. P. Oliveira, R. G. Oliveira-Junior, R. F. Santos, A. D. C. Santos, G. F. Tavares, J. R. G. S. Almeida
REVISTA VIRTUAL DE QUIMICA
(2019)
Article
Chemistry, Multidisciplinary
J. A. Bezerra, R. F. Correa, K. Salome, A. D. C. Santos, E. S. Lima, F. R. Campos, A. Barison, M. L. B. Pinheiro
REVISTA VIRTUAL DE QUIMICA
(2019)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
