Creation and preclinical evaluation of genetically attenuated malaria parasites arresting growth late in the liver

CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations

(2022) Ida Höijer et al.   Nature Communications

The challenges of a circumsporozoite protein-based malaria vaccine

(2021) Deepyan Chatterjee et al.   Expert Review of Vaccines

Plasmodium falciparum LipB mutants display altered redox and carbon metabolism in asexual stages and cannot complete sporogony in Anopheles mosquitoes

(2021) Marco Biddau et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Plasmodium Ape1 is a multifunctional enzyme in mitochondrial base excision repair and is required for efficient transition from liver to blood stage infection

(2021) Neetu Verma et al.   DNA REPAIR

Two chemoattenuated PfSPZ malaria vaccines induce sterile hepatic immunity

(2021) Agnes Mwakingwe-Omari et al.   NATURE

Safety, immunogenicity and efficacy of PfSPZ Vaccine against malaria in infants in western Kenya: a double-blind, randomized, placebo-controlled phase 2 trial

(2021) Martina Oneko et al.   NATURE MEDICINE

Safety and efficacy of a three-dose regimen of Plasmodium falciparum sporozoite vaccine in adults during an intense malaria transmission season in Mali: a randomised, controlled phase 1 trial

(2021) Mahamadou S Sissoko et al.   LANCET INFECTIOUS DISEASES

Strains used in whole organism Plasmodium falciparum vaccine trials differ in genome structure, sequence, and immunogenic potential

(2020) Kara A. Moser et al.   Genome Medicine

Safety Considerations for Malaria Volunteer Infection Studies: A Mini-Review

(2020) Anand Odedra et al.   AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

CRISPR gene editing in human embryos wreaks chromosomal mayhem

(2020) Heidi Ledford   NATURE

Generation of Novel Plasmodium falciparum NF135 and NF54 Lines Expressing Fluorescent Reporter Proteins Under the Control of Strong and Constitutive Promoters

(2020) Shinya Miyazaki et al.   Frontiers in Cellular and Infection Microbiology

Malaria vaccines since 2000: progress, priorities, products

(2020) Patrick E. Duffy et al.   npj Vaccines

A replication-competent late liver stage–attenuated human malaria parasite

(2020) Debashree Goswami et al.   JCI Insight

Improvement of CRISPR/Cas9 system by transfecting Cas9-expressing Plasmodium berghei with linear donor template

(2020) Naoaki Shinzawa et al.   Communications Biology

Beyond Blood Smears—Qualification of the Plasmodium 18S rRNA as a Biomarker for Controlled Human Malaria Infections

(2019) Annette M. Seilie et al.   AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

A P. falciparum NF54 Reporter Line Expressing mCherry-Luciferase in Gametocytes, Sporozoites, and Liver-Stages

(2019) Catherin Marin-Mogollon et al.   Frontiers in Cellular and Infection Microbiology

Vaccination With Sporozoites: Models and Correlates of Protection

(2019) Yun Shan Goh et al.   Frontiers in Immunology

Genome-Scale Identification of Essential Metabolic Processes for Targeting the Plasmodium Liver Stage

(2019) Rebecca R. Stanway et al.   CELL

Safety, Immunogenicity, and Protective Efficacy against Controlled Human Malaria Infection of Plasmodium falciparum Sporozoites Vaccine in Tanzanian Adults

(2018) Said A. Jongo et al.   AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

A Plasmodium Parasite with Complete Late Liver Stage Arrest Protects against Preerythrocytic and Erythrocytic Stage Infection in Mice

(2018) Ashley M. Vaughan et al.   INFECTION AND IMMUNITY

CRISPR/Cas9 system in Plasmodium falciparum using the centromere plasmid

(2018) Tongchai Payungwoung et al.   PARASITOLOGY INTERNATIONAL

Host biotin is required for liver stage development in malaria parasites

(2018) Teegan A. Dellibovi-Ragheb et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

OX40 Stimulation Enhances Protective Immune Responses Induced After Vaccination With Attenuated Malaria Parasites

(2018) Ahmad Syibli Othman et al.   Frontiers in Cellular and Infection Microbiology

Multiplex real-time PCR for diagnosing malaria in a non-endemic setting: a prospective comparison to conventional methods

(2018) R. H. T. Nijhuis et al.   EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES

Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

(2018) Catherin Marin-Mogollon et al.   MALARIA JOURNAL

Designer Parasites: Genetically Engineered Plasmodium as Vaccines To Prevent Malaria Infection

(2018) Debashree Goswami et al.   JOURNAL OF IMMUNOLOGY

Safety and efficacy of PfSPZ Vaccine against Plasmodium falciparum via direct venous inoculation in healthy malaria-exposed adults in Mali: a randomised, double-blind phase 1 trial

(2017) Mahamadou S Sissoko et al.   LANCET INFECTIOUS DISEASES

Humoral protection against mosquito bite-transmitted Plasmodium falciparum infection in humanized mice

(2017) Brandon K. Sack et al.   npj Vaccines

Development of whole sporozoite malaria vaccines

(2016) Michael R. Hollingdale et al.   Expert Review of Vaccines

A Plasmodium yoelii Mei2-Like RNA Binding Protein Is Essential for Completion of Liver Stage Schizogony

(2016) Dorender A. Dankwa et al.   INFECTION AND IMMUNITY

CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering

(2016) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

Functional profiles of orphan membrane transporters in the life cycle of the malaria parasite

(2016) Sanketha Kenthirapalan et al.   Nature Communications

Protective efficacy and safety of liver stage attenuated malaria parasites

(2016) Hirdesh Kumar et al.   Scientific Reports

Variant Exported Blood-Stage Proteins Encoded by Plasmodium Multigene Families Are Expressed in Liver Stages Where They Are Exported into the Parasitophorous Vacuole

(2016) Aurélie Fougère et al.   PLoS Pathogens

UDP-galactose and acetyl-CoA transporters as Plasmodium multidrug resistance genes

(2016) Michelle Yi-Xiu Lim et al.   Nature Microbiology

Rapid Generation of Marker-Free P. falciparum Fluorescent Reporter Lines Using Modified CRISPR/Cas9 Constructs and Selection Protocol

(2016) Catherin Marin Mogollon et al.   PLoS One

HISAT: a fast spliced aligner with low memory requirements

(2015) Daehwan Kim et al.   NATURE METHODS

PlasmoGEM, a database supporting a community resource for large-scale experimental genetics in malaria parasites

(2015) Frank Schwach et al.   NUCLEIC ACIDS RESEARCH

Progress with Plasmodium falciparum sporozoite (PfSPZ)-based malaria vaccines

(2015) Thomas L. Richie et al.   VACCINE

Novel approaches to whole sporozoite vaccination against malaria

(2015) Else M. Bijker et al.   VACCINE

A semi-automated luminescence based standard membrane feeding assay identifies novel small molecules that inhibit transmission of malaria parasites by mosquitoes

(2015) Martijn W. Vos et al.   Scientific Reports

Trimmomatic: a flexible trimmer for Illumina sequence data

(2014) Anthony M. Bolger et al.   BIOINFORMATICS

ZIPCO, a putative metal ion transporter, is crucial for Plasmodium liver-stage development

(2014) T. Sahu et al.   EMBO Molecular Medicine

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

(2014) Mehdi Ghorbal et al.   NATURE BIOTECHNOLOGY

A genetically attenuated malaria vaccine candidate based on P. falciparum b9/slarp gene-deficient sporozoites

(2014) Ben C L van Schaijk et al.   eLife

Type II Fatty Acid Biosynthesis Is Essential for Plasmodium falciparum Sporozoite Development in the Midgut of Anopheles Mosquitoes

(2013) Ben C. L. van Schaijk et al.   EUKARYOTIC CELL

A new approach to generate a safe double-attenuated Plasmodium liver stage vaccine

(2013) Andreas Nagel et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Enzymes involved in plastid-targeted phosphatidic acid synthesis are essential forPlasmodium yoeliiliver-stage development

(2013) Scott E. Lindner et al.   MOLECULAR MICROBIOLOGY

Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration

(2012) H. Thorvaldsdottir et al.   BRIEFINGS IN BIOINFORMATICS

Complete Plasmodium falciparum liver-stage development in liver-chimeric mice

(2012) Ashley M. Vaughan et al.   JOURNAL OF CLINICAL INVESTIGATION

NF135.C10: A New Plasmodium falciparum Clone for Controlled Human Malaria Infections

(2012) Anne C. Teirlinck et al.   JOURNAL OF INFECTIOUS DISEASES

Improved negative selection protocol for Plasmodium berghei in the rodent malarial model

(2012) Rachael Y Orr et al.   MALARIA JOURNAL

Liver-specific protein 2: a Plasmodium protein exported to the hepatocyte cytoplasm and required for merozoite formation

(2012) Yuki Orito et al.   MOLECULAR MICROBIOLOGY

Identification of an AP2-family Protein That Is Critical for Malaria Liver Stage Development

(2012) Shiroh Iwanaga et al.   PLoS One

Assessing the adequacy of attenuation of genetically modified malaria parasite vaccine candidates

(2012) Takeshi Annoura et al.   VACCINE

Superior Antimalarial Immunity after Vaccination with Late Liver Stage-Arresting Genetically Attenuated Parasites

(2011) Noah S. Butler et al.   Cell Host & Microbe

Inactivation of a Plasmodium apicoplast protein attenuates formation of liver merozoites

(2011) Joana M. Haussig et al.   MOLECULAR MICROBIOLOGY

Live Attenuated Malaria Vaccine Designed to Protect Through Hepatic CD8+ T Cell Immunity

(2011) J. E. Epstein et al.   SCIENCE

The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data

(2010) A. McKenna et al.   GENOME RESEARCH

Development of a metabolically active, non-replicating sporozoite vaccine to preventPlasmodium falciparummalaria

(2010) Stephen L. Hoffman et al.   Human vaccines & immunotherapeutics

Removal of Heterologous Sequences from Plasmodium falciparum Mutants Using FLPe-Recombinase

(2010) Ben C. L. van Schaijk et al.   PLoS One

Plasmepsin 4-Deficient Plasmodium berghei Are Virulence Attenuated and Induce Protective Immunity against Experimental Malaria

(2009) Roberta Spaccapelo et al.   AMERICAN JOURNAL OF PATHOLOGY

Fast and accurate short read alignment with Burrows-Wheeler transform

(2009) H. Li et al.   BIOINFORMATICS

The Plasmodium serine-type SERA proteases display distinct expression patterns and non-essential in vivo roles during life cycle progression of the malaria parasite

(2009) Elyzana D. Putrianti et al.   CELLULAR MICROBIOLOGY

LISP1 is important for the egress ofPlasmodium bergheiparasites from liver cells

(2009) Tomoko Ishino et al.   CELLULAR MICROBIOLOGY

The Fatty Acid Biosynthesis Enzyme FabI Plays a Key Role in the Development of Liver-Stage Malarial Parasites

(2008) Min Yu et al.   Cell Host & Microbe

Type II fatty acid synthesis is essential only for malaria parasite late liver stage development

(2008) Ashley M. Vaughan et al.   CELLULAR MICROBIOLOGY

Plasmodium falciparum: Food vacuole localization of nitric oxide-derived species in intraerythrocytic stages of the malaria parasite

(2008) Graciela Ostera et al.   EXPERIMENTAL PARASITOLOGY