Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system

Prespacers formed during primed adaptation associate with the Cas1–Cas2 adaptation complex and the Cas3 interference nuclease–helicase

(2021) Olga Musharova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Two mechanisms of chromosome fragility at replication-termination sites in bacteria

(2021) Qian Mei et al.   Science Advances

Selective loading and processing of prespacers for precise CRISPR adaptation

(2020) Sungchul Kim et al.   NATURE

Primed CRISPR DNA uptake in Pyrococcus furiosus

(2020) Sandra Garrett et al.   NUCLEIC ACIDS RESEARCH

Reproducible Antigen Recognition by the Type I-F CRISPR-Cas System

(2020) Tanner Wiegand et al.   CRISPR Journal

The Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation

(2019) Hayun Lee et al.   eLife

Fidelity of prespacer capture and processing is governed by the PAM mediated interactions of Cas1-2 adaptation complex in CRISPR-Cas type I-E system

(2019) Kakimani Nagarajan Yoganand et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Detection of spacer precursors formed in vivo during primed CRISPR adaptation

(2019) Anna A. Shiriaeva et al.   Nature Communications

Genome Maintenance Proteins Modulate Autoimmunity Mediated Primed Adaptation by the Escherichia coli Type I-E CRISPR-Cas System

(2019) Elena Kurilovich et al.   Genes

Processing and integration of functionally oriented prespacers in the E. coli CRISPR system depends on bacterial host exonucleases

(2019) Anita Ramachandran et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

DnaQ exonuclease‐like domain of Cas2 promotes spacer integration in a type I‐E CRISPR‐Cas system

(2018) Gediminas Drabavicius et al.   EMBO REPORTS

Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays

(2018) Hayun Lee et al.   MOLECULAR CELL

Structure basis for RNA-guided DNA degradation by Cascade and Cas3

(2018) Yibei Xiao et al.   SCIENCE

Assembly and Translocation of a CRISPR-Cas Primed Acquisition Complex

(2018) Kaylee E. Dillard et al.   CELL

Prespacer processing and specific integration in a Type I-A CRISPR system

(2017) Clare Rollie et al.   NUCLEIC ACIDS RESEARCH

Sequential eviction of crowded nucleoprotein complexes by the exonuclease RecBCD molecular motor

(2017) Tsuyoshi Terakawa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Static and Dynamic Factors Limit Chromosomal Replication Complexity in Escherichia coli, Avoiding Dangers of Runaway Overreplication

(2016) S. R. Khan et al.   GENETICS

CRISPR Immunological Memory Requires a Host Factor for Specificity

(2016) James K. Nuñez et al.   MOLECULAR CELL

DNA Breaks and End Resection Measured Genome-wide by End Sequencing

(2016) Andres Canela et al.   MOLECULAR CELL

Molecular recordings by directed CRISPR spacer acquisition

(2016) Seth L. Shipman et al.   SCIENCE

Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome

(2016) Hafez El Sayyed et al.   PLoS Genetics

Holliday junction trap shows how cells use recombination and a junction-guardian role of RecQ helicase

(2016) J. Xia et al.   Science Advances

Methods that remove batch effects while retaining group differences may lead to exaggerated confidence in downstream analyses

(2015) Vegard Nygaard et al.   BIOSTATISTICS

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems

(2015) Jiuyu Wang et al.   CELL

Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

Foreign DNA capture during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

Foreign DNA acquisition by the I-F CRISPR–Cas system requires all components of the interference machinery

(2015) Daria Vorontsova et al.   NUCLEIC ACIDS RESEARCH

Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity

(2014) James K Nuñez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference

(2014) M. L. Hochstrasser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Completion of DNA replication inEscherichia coli

(2014) Brian M. Wendel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus

(2013) Tomas Sinkunas et al.   EMBO JOURNAL

In VitroReconstitution of anEscherichia coliRNA-guided Immune System Reveals Unidirectional, ATP-dependent Degradation of DNA Target

(2013) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3

(2012) Edze R. Westra et al.   MOLECULAR CELL

Mechanism of Foreign DNA Selection in a Bacterial Adaptive Immune System

(2012) Dipali G. Sashital et al.   MOLECULAR CELL

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli

(2012) Ido Yosef et al.   NUCLEIC ACIDS RESEARCH

CRISPR Interference Directs Strand Specific Spacer Acquisition

(2012) Daan C. Swarts et al.   PLoS One

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

(2012) Kirill A. Datsenko et al.   Nature Communications

Structural basis for CRISPR RNA-guided DNA recognition by Cascade

(2011) Matthijs M Jore et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Single-molecule imaging reveals mechanisms of protein disruption by a DNA translocase

(2010) Ilya J. Finkelstein et al.   NATURE

ShortRead: a bioconductor package for input, quality assessment and exploration of high-throughput sequence data

(2009) M. Morgan et al.   BIOINFORMATICS

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Prokaryotic silencing (psi)RNAs in Pyrococcus furiosus

(2008) C. Hale et al.   RNA

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE