Selective loading and processing of prespacers for precise CRISPR adaptation

Detection of spacer precursors formed in vivo during primed CRISPR adaptation

(2019) Anna A. Shiriaeva et al.   Nature Communications

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

DnaQ exonuclease‐like domain of Cas2 promotes spacer integration in a type I‐E CRISPR‐Cas system

(2018) Gediminas Drabavicius et al.   EMBO REPORTS

Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays

(2018) Hayun Lee et al.   MOLECULAR CELL

Repetitive DNA Reeling by the Cascade-Cas3 Complex in Nucleotide Unwinding Steps

(2018) Luuk Loeff et al.   MOLECULAR CELL

Cas4 Nucleases Define the PAM, Length, and Orientation of DNA Fragments Integrated at CRISPR Loci

(2018) Masami Shiimori et al.   MOLECULAR CELL

Insights into a Mysterious CRISPR Adaptation Factor, Cas4

(2018) Zhonggang Hou et al.   MOLECULAR CELL

Cas4 Facilitates PAM-Compatible Spacer Selection during CRISPR Adaptation

(2018) Sebastian N. Kieper et al.   Cell Reports

DNA-based memory devices for recording cellular events

(2018) Ravi U. Sheth et al.   NATURE REVIEWS GENETICS

Molecular mechanisms of CRISPR–Cas spacer acquisition

(2018) Jon McGinn et al.   NATURE REVIEWS MICROBIOLOGY

Assembly and Translocation of a CRISPR-Cas Primed Acquisition Complex

(2018) Kaylee E. Dillard et al.   CELL

How type II CRISPR–Cas establish immunity through Cas1–Cas2-mediated spacer integration

(2017) Yibei Xiao et al.   NATURE

Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation

(2017) Olga Musharova et al.   NUCLEIC ACIDS RESEARCH

Prespacer processing and specific integration in a Type I-A CRISPR system

(2017) Clare Rollie et al.   NUCLEIC ACIDS RESEARCH

CRISPR-Cas: Adapting to change

(2017) Simon A. Jackson et al.   SCIENCE

Single-molecule visualization of fast polymerase turnover in the bacterial replisome

(2017) Jacob S Lewis et al.   eLife

CRISPR Immunological Memory Requires a Host Factor for Specificity

(2016) James K. Nuñez et al.   MOLECULAR CELL

Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation

(2016) Tim Künne et al.   MOLECULAR CELL

Highly efficient primed spacer acquisition from targets destroyed by theEscherichia colitype I-E CRISPR-Cas interfering complex

(2016) Ekaterina Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System

(2015) Sy Redding et al.   CELL

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems

(2015) Jiuyu Wang et al.   CELL

Temporal Landscape of MicroRNA-Mediated Host-Virus Crosstalk during Productive Human Cytomegalovirus Infection

(2015) Sungchul Kim et al.   Cell Host & Microbe

Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

Foreign DNA capture during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

CRISPR adaptation biases explain preference for acquisition of foreign DNA

(2015) Asaf Levy et al.   NATURE

Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity

(2014) James K Nuñez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Pervasive generation of oppositely oriented spacers during CRISPR adaptation

(2014) Sergey Shmakov et al.   NUCLEIC ACIDS RESEARCH

A direct proofreader–clamp interaction stabilizes the Pol III replicase in the polymerization mode

(2013) Slobodan Jergic et al.   EMBO JOURNAL

In VitroReconstitution of anEscherichia coliRNA-guided Immune System Reveals Unidirectional, ATP-dependent Degradation of DNA Target

(2013) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

High-throughput analysis of type I-E CRISPR/Cas spacer acquisition inE. coli

(2013) Ekaterina Savitskaya et al.   RNA Biology

The highly dynamic CRISPR1 system ofStreptococcus agalactiaecontrols the diversity of its mobilome

(2012) Maria-José Lopez-Sanchez et al.   MOLECULAR MICROBIOLOGY

Recombination Hotspots and Single-Stranded DNA Binding Proteins Couple DNA Translocation to DNA Unwinding by the AddAB Helicase-Nuclease

(2011) Joseph T.P. Yeeles et al.   MOLECULAR CELL

Quick and Clean Cloning: A Ligation-Independent Cloning Strategy for Selective Cloning of Specific PCR Products from Non-Specific Mixes

(2011) Frank Thieme et al.   PLoS One

The AddAB helicase–nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain

(2010) Joseph T.P. Yeeles et al.   NUCLEIC ACIDS RESEARCH

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE