The Cas4-Cas1-Cas2 complex mediates precise prespacer processing during CRISPR adaptation

Cas4 nucleases can effect specific integration of CRISPR spacers

(2019) Zhufeng Zhang et al.   JOURNAL OF BACTERIOLOGY

Cas4–Cas1 fusions drive efficient PAM selection and control CRISPR adaptation

(2019) Cristóbal Almendros et al.   NUCLEIC ACIDS RESEARCH

Cas4-Dependent Prespacer Processing Ensures High-Fidelity Programming of CRISPR Arrays

(2018) Hayun Lee et al.   MOLECULAR CELL

Cas4 Nucleases Define the PAM, Length, and Orientation of DNA Fragments Integrated at CRISPR Loci

(2018) Masami Shiimori et al.   MOLECULAR CELL

Cas4 Facilitates PAM-Compatible Spacer Selection during CRISPR Adaptation

(2018) Sebastian N. Kieper et al.   Cell Reports

How type II CRISPR–Cas establish immunity through Cas1–Cas2-mediated spacer integration

(2017) Yibei Xiao et al.   NATURE

Prespacer processing and specific integration in a Type I-A CRISPR system

(2017) Clare Rollie et al.   NUCLEIC ACIDS RESEARCH

Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus

(2017) Tao Liu et al.   NUCLEIC ACIDS RESEARCH

CRISPR-Cas: Adapting to change

(2017) Simon A. Jackson et al.   SCIENCE

Scipion: A software framework toward integration, reproducibility and validation in 3D electron microscopy

(2016) J.M. de la Rosa-Trevín et al.   JOURNAL OF STRUCTURAL BIOLOGY

Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System

(2015) Sy Redding et al.   CELL

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems

(2015) Jiuyu Wang et al.   CELL

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity

(2015) Eva Nogales et al.   MOLECULAR CELL

Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

Foreign DNA capture during CRISPR–Cas adaptive immunity

(2015) James K. Nuñez et al.   NATURE

CRISPR-Cas immunity in prokaryotes

(2015) Luciano A. Marraffini   NATURE

The Phyre2 web portal for protein modeling, prediction and analysis

(2015) Lawrence A Kelley et al.   Nature Protocols

Different genome stability proteins underpin primed and naïve adaptation inE. coliCRISPR-Cas immunity

(2015) Ivana Ivančić-Baće et al.   NUCLEIC ACIDS RESEARCH

Cutting it close: CRISPR-associated endoribonuclease structure and function

(2015) Megan L. Hochstrasser et al.   TRENDS IN BIOCHEMICAL SCIENCES

Dna2 nuclease-helicase structure, mechanism and regulation by Rpa

(2015) Chun Zhou et al.   eLife

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition

(2015) Clare Rollie et al.   eLife

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity

(2014) James K Nuñez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity

(2014) Sofia Lemak et al.   NUCLEIC ACIDS RESEARCH

Detection and characterization of spacer integration intermediates in type I-E CRISPR–Cas system

(2014) Zihni Arslan et al.   NUCLEIC ACIDS RESEARCH

CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference

(2014) M. L. Hochstrasser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal structure of Cas1 from Archaeoglobus fulgidus and characterization of its nucleolytic activity

(2013) Tae-Yang Kim et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A pattern matching approach to the automatic selection of particles from low-contrast electron micrographs

(2013) V. Abrishami et al.   BIOINFORMATICS

Particle quality assessment and sorting for automatic and semiautomatic particle-picking techniques

(2013) J. Vargas et al.   JOURNAL OF STRUCTURAL BIOLOGY

Toroidal Structure and DNA Cleavage by the CRISPR-Associated [4Fe-4S] Cluster Containing Cas4 Nuclease SSO0001 from Sulfolobus solfataricus

(2013) Sofia Lemak et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Adaptation of the Haloarcula hispanica CRISPR-Cas system to a purified virus strictly requires a priming process

(2013) Ming Li et al.   NUCLEIC ACIDS RESEARCH

Characterization of the CRISPR/Cas Subtype I-A System of the Hyperthermophilic Crenarchaeon Thermoproteus tenax

(2012) A. Plagens et al.   JOURNAL OF BACTERIOLOGY

Double-stranded Endonuclease Activity inBacillus haloduransClustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated Cas2 Protein

(2012) Ki Hyun Nam et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Mechanism of Foreign DNA Selection in a Bacterial Adaptive Immune System

(2012) Dipali G. Sashital et al.   MOLECULAR CELL

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

CRISPR Interference Directs Strand Specific Spacer Acquisition

(2012) Daan C. Swarts et al.   PLoS One

The CRISPR Associated Protein Cas4 Is a 5′ to 3′ DNA Exonuclease with an Iron-Sulfur Cluster

(2012) Jing Zhang et al.   PLoS One

Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system

(2012) Kirill A. Datsenko et al.   Nature Communications

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions

(2010) Grigore D. Pintilie et al.   JOURNAL OF STRUCTURAL BIOLOGY

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE