4.1 Article

Insecticidal activity of essential oil of Cannabis sativa against the immature and adult stages of Ctenocephalides felis felis

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BRAZILIAN COLL VETERINARY PARASITOLOGY
DOI: 10.1590/S1984-29612023003

Keywords

Flea; ectoparasite; mortality; pets; volatile oil; biocontrol

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The essential oil of Cannabis sativa was found to have insecticidal activity against fleas in all stages of development, with γ-elemene and caryophyllene oxide as the major compounds. This suggests the potential use of cannabis essential oil as an ectoparasiticide, especially for flea eggs and larvae.
Essential oil (EO) of Cannabis sativa (C. sativa) was evaluated against the egg, larval, pupal, and adult stages of the flea Ctenocephalides felis felis. The chemical composition of EO was determined by gas chromatography with flame ionization and mass spectrometry. EO mainly comprised gamma-elemene (16.2%) and caryophyllene oxide (14.2%) as major compounds. To evaluate the mortality of flea stages in vitro, filter paper tests were performed at different concentrations. EO of C. sativa showed insecticidal activity (100% mortality at the highest concentrations) for flea control at egg, larval, pupal, and adult stages, with lethal concentrations (LC50) of 32.45; 91.61; 466.41 and 927.92 mu g/cm2, respectively. EO of C. sativa indicated the potential for the development of ectoparasiticide for veterinary use, especially for fleas in egg and larval stages.

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