SHERLOCK4HAT: A CRISPR-based tool kit for diagnosis of Human African Trypanosomiasis

CRISPR-based diagnostics

(2021) Michael M. Kaminski et al.   Nature Biomedical Engineering

Single nucleotide polymorphisms and copy-number variations in the Trypanosoma brucei repeat (TBR) sequence can be used to enhance amplification and genotyping of Trypanozoon strains

(2021) Nick Van Reet et al.   PLoS One

Monitoring the elimination of human African trypanosomiasis at continental and country level: Update to 2018

(2020) José R. Franco et al.   PLoS Neglected Tropical Diseases

Extravascular dermal trypanosomes in suspected and confirmed cases of gambiense Human African Trypanosomiasis

(2020) Mariame Camara et al.   CLINICAL INFECTIOUS DISEASES

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Ultrasensitive CRISPR-based diagnostic for field-applicable detection ofPlasmodiumspecies in symptomatic and asymptomatic malaria

(2020) Rose A. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

(2020) Jon Arizti-Sanz et al.   Nature Communications

Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection

(2019) Stephen M. Chiweshe et al.   PLoS Neglected Tropical Diseases

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

RNA Binding and HEPN-Nuclease Activation Are Decoupled in CRISPR-Cas13a

(2018) Akshay Tambe et al.   Cell Reports

Human African trypanosomiasis

(2017) Philippe Büscher et al.   LANCET

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Population genomics reveals the origin and asexual evolution of human infective trypanosomes

(2016) William Weir et al.   eLife

How can molecular diagnostics contribute to the elimination of human African trypanosomiasis?

(2015) Philippe Büscher et al.   EXPERT REVIEW OF MOLECULAR DIAGNOSTICS

Sensitivity testing of trypanosome detection by PCR from whole blood samples using manual and automated DNA extraction methods

(2014) J. Dunlop et al.   EXPERIMENTAL PARASITOLOGY

Diagnosis of Trypanosomatid Infections

(2014) Pablo González-Andrade et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Rapid Diagnostic Test for Sleeping Sickness

(2013) Philippe Büscher et al.   NEW ENGLAND JOURNAL OF MEDICINE

Diagnostic Accuracy of Loopamp Trypanosoma brucei Detection Kit for Diagnosis of Human African Trypanosomiasis in Clinical Samples

(2013) Patrick Mitashi et al.   PLoS Neglected Tropical Diseases

Recent progress in molecular diagnosis of sleeping sickness

(2012) Stijn Deborggraeve et al.   EXPERT REVIEW OF MOLECULAR DIAGNOSTICS

The Human African Trypanosomiasis Specimen Biobank: A Necessary Tool to Support Research of New Diagnostics

(2012) Jose R. Franco et al.   PLoS Neglected Tropical Diseases

Diagnostic Accuracy of Molecular Amplification Tests for Human African Trypanosomiasis—Systematic Review

(2012) Claire M. Mugasa et al.   PLoS Neglected Tropical Diseases

Phylogeography and Taxonomy of Trypanosoma brucei

(2011) Oliver Balmer et al.   PLoS Neglected Tropical Diseases

Diagnostic Accuracy of PCR in gambiense Sleeping Sickness Diagnosis, Staging and Post-Treatment Follow-Up: A 2-year Longitudinal Study

(2011) Stijn Deborggraeve et al.   PLoS Neglected Tropical Diseases

Conserved sequence of the TgsGP gene in Group 1 Trypanosoma brucei gambiense

(2010) Wendy Gibson et al.   INFECTION GENETICS AND EVOLUTION

Sleeping sickness diagnosis: use of buffy coats improves the sensitivity of the mini anion exchange centrifugation test

(2010) Mamadou Camara et al.   TROPICAL MEDICINE & INTERNATIONAL HEALTH

Improved Models of Mini Anion Exchange Centrifugation Technique (mAECT) and Modified Single Centrifugation (MSC) for Sleeping Sickness Diagnosis and Staging

(2009) Philippe Büscher et al.   PLoS Neglected Tropical Diseases

Nucleic Acid Sequence-Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples

(2008) C. M. Mugasa et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid Detection of Trypanosoma brucei rhodesiense

(2008) Zablon Kithinji Njiru et al.   PLoS Neglected Tropical Diseases