Article
Chemistry, Analytical
Ekaterina Tsarenko, Ulrich S. Schubert, Ivo Nischang
Summary: Multifunctional nanoparticle formulations for medical purposes have been successfully developed, with a persistent challenge being the quantitative compositional analysis of the NPs with the polymer matrix and encapsulated drugs. In this study, PLGA NPs were formulated and their integrity and size were analyzed using DLS and SEM. Liquid chromatography on reversed-phase silica monolithic columns was used to quantitatively assess the amount of small molecule drugs and NP constituting PLGA polymer. The study demonstrates the viability of reversed-phase monolithic silica in chromatography, providing a valuable strategy for analysis of various precursor polymer systems and drug components in multifunctional drug formulations.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Chao Wang, Yu Liang, Xue Yang, Bowen Zhong, Xiaodan Zhang, Baofeng Zhao, Zhen Liang, Lihua Zhang, Yukui Zhang
Summary: A surface-charged ethane-bridged hybrid monolithic column was prepared for peptide separation, increasing peak capacity significantly and being compatible with MS mobile phases. This column allows efficient separation of Hela tryptic peptides and quantitative proteomic analysis in complex samples.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Applied
Ignacio Cabezudo, Mario O. Salazar, I. Ayelen Ramallo, Ricardo L. E. Furlan
Summary: Thin-layer chromatography (TLC) is commonly used for food analysis and quality control. It is compatible with various derivatization methods and can detect compounds related to food stability, contaminants, and nutrition.
Article
Chemistry, Multidisciplinary
Joon-Kyu Han, Jung-Woo Lee, Yeeun Kim, Young Bin Kim, Seong-Yun Yun, Sang-Won Lee, Ji-Man Yu, Keon Jae Lee, Hyun Myung, Yang-Kyu Choi
Summary: Neuromorphic hardware with a spiking neural network (SNN) can greatly improve the energy efficiency of artificial intelligence (AI) functions. This study demonstrates a neuromorphic module composed of synapses over neurons, achieved through monolithic vertical integration. By using techniques such as laser annealing and thermal annealing, the performance of the module is enhanced.
Article
Chemistry, Physical
S. Erkan, A. Yagmyrov, A. Altuntepe, R. Zan, M. A. Olgar
Summary: In this study, CZTS samples were produced on Mo and graphene/Mo coated glass substrates. The CZTS thin films were annealed in sulphur atmosphere using rapid thermal processing method. The results showed that using graphene as an inter-layer improved the crystalline quality and grain size of the CZTS films, leading to enhanced conversion efficiency of the solar cells.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Article
Chemistry, Multidisciplinary
Sawanta S. Mali, Jyoti V. Patil, Julian A. Steele, Mohammad Khaja Nazeeruddin, Jin Hyeok Kim, Chang Kook Hong
Summary: This study presents a monolithic hybrid tandem solar cell with an all-organic blend as the rear subcell, using metal-ion doped all-inorganic perovskite front subcells and a solution-processed ternary organic blend rear subcell. The use of dynamic hot-air deposition technology allows the fabrication and testing of the solar cells entirely in the open air, and passivation and selection layer treatments improve efficiency and reduce open-circuit voltage loss. The solar cells achieved a maximum efficiency of 23.07% and retained over 90% of the initial efficiency after 600 hours of continuous illumination.
ENERGY & ENVIRONMENTAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Xin Zhu, Xiaojie Li, Chaoming Gu, Zhi Ye, Zhen Cao, Xingye Zhang, Chuanhong Jin, Yang Liu
Summary: This study introduces and fabricates solid-state nanopore devices with solution-gated TFTs integrated inside for charge-based sensing of biomolecules. Experimental results demonstrate that TFT signals can accurately detect molecules with different charges, and the induced mirror charge can be inferred from the signal amplitudes.
Review
Chemistry, Analytical
Petr Chocholous, Katerina Matousova, Dalibor Satinsky, Lenka Kujovska Kremova, Hana Sklenarova
Summary: This article reviews the practical aspects of monolithic silica columns in HPLC and medium-pressure sequential injection chromatography (SIC) that may not be visible at first sight but are evident retrospectively.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Tawheed Hashem, Elvia P. Valadez Sanchez, Evgenia Bogdanova, Anna Ugodchikova, Alaa Mohamed, Matthias Schwotzer, Mohamed H. Alkordi, Christof Woell
Summary: Metal-organic frameworks (MOFs) are intensively explored for novel applications and as alternatives to existing materials in thin film nanotechnologies. The stability of SURMOFs in harsh aqueous environments varies, with UiO-66-NH2 SURMOFs showing stability over a large window of pH values.
Article
Multidisciplinary Sciences
Beata Polak, Emilia Pajurek
Summary: The research focused on separating water- and fat-soluble vitamins using micellar systems of HPTLC and PPEC, investigating the influence of mobile phase composition on migration distances and zone separation. Comparisons between HPLC and PPEC showed differences in system capacity, with PPEC performing better for fat-soluble vitamins and exhibiting lower variances and reproducibilities.
SCIENTIFIC REPORTS
(2021)
Article
Electrochemistry
Ortal Lidor-Shalev, Nicole Leifer, Michal Ejgenberg, Hagit Aviv, Ilana Perelshtein, Gil Goobes, Malachi Noked, Rosy
Summary: The study involves coating LiCoO2 (LCO) with hybrid organic-inorganic alucone thin films to enable high voltage operation, improve electrochemical performance, cycling stability, and specific capacity. Among the films studied, the 10nm ethylene glycol/Al coated LCO showed the best results.
BATTERIES & SUPERCAPS
(2021)
Article
Biophysics
Yu-Ling Chang, I-Chun Lai, Li-Chia Lu, Sih-Wei Chang, Aileen Y. Sun, Dehui Wan, Hsuen-Li Chen
Summary: In this study, a large-area, ultrasensitive SERS substrate was prepared using silver nanopaste. The substrate demonstrated high sensitivity, signal uniformity, and applicability for on-site detection when combined with a portable Raman spectrometer.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Integrative & Complementary Medicine
Yao Shuai, Zhang Jian-Qing, Hou Jin-Jun, Hu Xiao-Su, Wang Ling, Da Juan, Rao Wei, Wang Dan-Dan, Huang Yong, Wu Wan-Ying, Guo De-An
Summary: A simple and practical HPTLC-QDa method was developed for the robust authentication of Ganoderma lucidum and its confused species, allowing for efficient identification based on characteristic mass spectra.
CHINESE JOURNAL OF NATURAL MEDICINES
(2021)
Article
Materials Science, Multidisciplinary
Sun Kyung Hwang, Ik Jae Park, Se Won Seo, Jae Hyun Park, So Jeong Park, Jin Young Kim
Summary: We demonstrate an efficient monolithic two-terminal perovskite/CZTSSe tandem solar cell by reducing the surface roughness of the electrochemically deposited CZTSSe bottom cell. The perovskite top cell with a bandgap of 1.65 eV is prepared on the flattened CZTSSe bottom cell, resulting in efficient perovskite/CZTSSe tandem solar cells. The best performing tandem device exhibits a high conversion efficiency of 17.5% without the hysteresis effect.
ENERGY & ENVIRONMENTAL MATERIALS
(2023)
Article
Spectroscopy
M. Rizk, Zainab M. Mahmoud, Marwa M. Azab
Summary: Simple, economic, and selective spectrofluorimetric and thin layer chromatographic methods were developed for the determination of Cabergoline. Method A measured the fluorescence intensity at 338nm after excitation at 280nm, while method B used TLC silica gel plates exposed to concentrated hydrochloric acid vapor. Both methods were successfully validated according to ICH guidelines.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Oncology
Amanda M. Clark, Nancy L. Allbritton, Alan Wells
Summary: The liver is the most commonly involved organ in metastases from various solid tumors, and studying tumor cell behavior in the liver microenvironment has provided unexpected insights into the biology of metastasis. Microphysiological systems linking different organs can help examine the influence of one organ on the function of other tissues, including how the liver environment affects tumor behavior.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Biochemical Research Methods
Samuel S. Hinman, Yuli Wang, Raehyun Kim, Nancy L. Allbritton
Summary: This protocol describes the fabrication of 3D collagen scaffolds used to culture human colonic crypts derived from primary intestinal epithelial cells. The methods include microfabrication and soft lithography protocols necessary to shape hydrogels to match in vivo crypt dimensions. The platform supports the formation of chemical gradients across the crypts, including growth and differentiation factors, inflammatory compounds, bile and food metabolites and bacterial products.
Article
Engineering, Biomedical
Samuel S. Hinman, Jennifer Huling, Yuli Wang, Hao Wang, Ross C. Bretherton, Cole A. deForest, Nancy L. Allbritton
Summary: The study utilized magnetic agarose hydrogels to mimic the propulsion of solid fecal contents over colonic epithelium, without adversely affecting cell monolayer surface coverage, viability, or transepithelial electrical resistance, but impacting protein secretion and cell morphology.
Review
Biotechnology & Applied Microbiology
Cody A. LaBelle, Angelo Massaro, Belen Cortes-Llanos, Christopher E. Sims, Nancy L. Allbritton
Summary: Single-cell analysis and sorting have revolutionized biomedical research and clinical medicine, allowing for a deeper exploration of cell physiology and behavior. These technologies provide powerful tools for genetic engineering, regenerative medicine, and cancer immunotherapies by enabling more precise cell selection criteria and the creation of cell lines with unique properties.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Sebastian Mestril, Raehyun Kim, Samuel S. Hinman, Shawn M. Gomez, Nancy L. Allbritton
Summary: The study demonstrated that colonic epithelial cultures enriched for stem/proliferative cells exhibit higher frequency and shorter pulse width of Ca2+ transients, and differentiation leads to a decrease in transient frequency by 50% and an increase in pulse width by 200%. Various metabolites and growth factors can modulate Ca2+ oscillation patterns consistently, while increasing the stiffness of the supportive matrix shifts Ca2+ transient patterns toward those resembling differentiated cells.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Analytical
Matthew M. Anttila, Brianna M. Vickerman, Qunzhao Wang, David S. Lawrence, Nancy L. Allbritton
Summary: Peptide bioreporters have been developed to measure the activation of EGFR, Akt, and proteases/peptidases in single cells simultaneously, with the optimized reporter allowing for quantification of multiple enzyme activities. A photoactivatable version of the reporter has been synthesized, enabling temporally controlled measurement of kinase signaling and proteolytic degradation in single cells. This work will facilitate the development of a new generation of multiplexed activity-based reporters for light-initiated measurement of enzymatic activity in single cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Luke A. Gallion, Yuli Wang, Angelo Massaro, Ming Yao, Brae Petersen, Quanzheng Zhang, Weigang Huang, Adam J. Carr, Qisheng Zhang, Nancy L. Allbritton
Summary: Capillary electrophoresis with fluorescence detection (CE-F) is a powerful method for measuring enzyme activation in single cells. However, the reporter substrates used in cellular enzymatic assays for CE-F may affect enzyme kinetics. To address this issue, a fix and click method was developed to attach a fluorophore to an enzyme activation reporter prior to analysis by CE-F, allowing for the investigation of sphingolipid signaling in single cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Cecilia Villegas-Novoa, Yuli Wang, Christopher E. Sims, Nancy L. Allbritton
Summary: This paper describes an optimized analytical assay pipeline for screening compounds to modulate L-cell signaling. The pipeline utilizes a human intestinal tissue construct enriched in L-cells and standard antibody-based GLP-1 assays to quantify GLP-1 secretion. Additionally, by adding specific compounds and altering the culture conditions, the number of L-cells is increased to improve the signal-to-noise ratio of conventional enzyme-linked immunoassays.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yuli Wang, Christopher E. Sims, Nancy L. Allbritton
Summary: This study developed a 2D crypt model based on intestinal stem cells for effective screening of drug compounds on the intestinal epithelium. The model accurately mimics the physiological characteristics of intestinal tissue and allows for quantitative assessment of various cellular parameters using immunofluorescence-based assay. It can be used for compound screening.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Cody A. LaBelle, Raymond J. Zhang, Sally A. Hunsucker, Paul M. Armistead, Nancy L. Allbritton
Summary: Chimeric antigen receptor T (CAR-T) cell immunotherapies have achieved success in treating hematological malignancies, but their efficacy can vary significantly. This study presents an automated microraft array platform that tracks and measures the killing behavior and motility of CAR-T cells, and allows for the collection of single cells for further characterization. The results demonstrate the heterogeneity in killing behavior among CAR-T cells in a cell population.
Article
Materials Science, Biomaterials
Samuel S. Hinman, Angelo Massaro, Yuli Wang, Christopher E. Sims, Raehyun Kim, Nancy L. Allbritton
Summary: This study describes a method to create an in vitro model of human colon physiology with a collagen hydrogel scaffold, allowing co-culture of primary gastrointestinal epithelium and immune cells. The model demonstrates that it possesses a functional primary colonic epithelial layer with a recruitable immune cell compartment in response to pro-inflammatory cues.
Article
Chemistry, Analytical
Matthew DiSalvo, Belen Cortes-Llanos, Cody LaBelle, David Murdoch, Nancy Allbritton
Summary: This work introduces a tool-free technique for batch construction of composite microstructures. The method allows control over the volumes and positions of deposited materials within the microstructures. It has the potential to enable the fabrication of complex composite microelements for various applications.
Article
Chemistry, Analytical
Yuli Wang, Christopher E. Sims, Nancy L. Allbritton
Summary: A planar, self-renewing human intestinal monolayer platform was developed to screen the effects of compounds on the intestinal epithelium. Using an immunofluorescence-based assay, the model can assess cell characteristics and identify compounds that significantly impact cell proliferation and differentiation.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemical Research Methods
Belen Cortes-Llanos, Yuli Wang, Christopher E. Sims, Nancy L. Allbritton
Summary: Microraft arrays are a versatile platform designed for the analysis and isolation of biological entities, offering a wide range of applications such as single-cell RNA sequencing, high-throughput screening, and development of CRISPR gene-edited cell lines. The technology is elegant in its simplicity and allows for flexible selection criteria not possible with conventional sorting methods, garnering significant interest in the biological and pharmaceutical sciences.