The pleiotropic roles of SPT5 in transcription

Spt5 histone binding activity preserves chromatin during transcription by RNA polymerase II

(2022) Cecile Evrin et al.   EMBO JOURNAL

Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF

(2022) Vladyslava Gorbovytska et al.   Nature Communications

RPAP2 regulates a transcription initiation checkpoint by inhibiting assembly of pre-initiation complex

(2022) Xinxin Wang et al.   Cell Reports

The PAF1 complex promotes 3′ processing of pervasive transcripts

(2022) Xinhong Liu et al.   Cell Reports

Coordinated regulation of RNA polymerase II pausing and elongation progression by PAF1

(2022) Zhenning Wang et al.   Science Advances

Integrator is a genome-wide attenuator of non-productive transcription

(2021) Søren Lykke-Andersen et al.   MOLECULAR CELL

Regulation of Promoter Proximal Pausing of RNA Polymerase II in Metazoans

(2021) Roberta Dollinger et al.   JOURNAL OF MOLECULAR BIOLOGY

Acute BAF perturbation causes immediate changes in chromatin accessibility

(2021) Sandra Schick et al.   NATURE GENETICS

Mammalian SWI/SNF continuously restores local accessibility to chromatin

(2021) Mario Iurlaro et al.   NATURE GENETICS

The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer

(2021) Stephin J. Vervoort et al.   CELL

Protein phosphatases in the RNAPII transcription cycle: erasers, sculptors, gatekeepers, and potential drug targets

(2021) Giacomo Cossa et al.   GENES & DEVELOPMENT

Structural basis of nucleosome transcription mediated by Chd1 and FACT

(2021) Lucas Farnung et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Opposite roles of transcription elongation factors Spt4/5 and Elf1 in RNA polymerase II transcription through B-form versus non-B DNA structures

(2021) Jun Xu et al.   NUCLEIC ACIDS RESEARCH

Two distinct mechanisms of RNA polymerase II elongation stimulation in vivo

(2021) Kristina Žumer et al.   MOLECULAR CELL

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape

(2021) Shibin Hu et al.   MOLECULAR CELL

SPT5 stabilization of promoter-proximal RNA polymerase II

(2021) Yuki Aoi et al.   MOLECULAR CELL

Structural basis of human transcription–DNA repair coupling

(2021) Goran Kokic et al.   NATURE

Biochemical insights into Paf1 complex–induced stimulation of Rad6/Bre1-mediated H2B monoubiquitination

(2021) Feilong Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Diversity and Emerging Roles of Enhancer RNA in Regulation of Gene Expression and Cell Fate

(2020) Preston R. Arnold et al.   Frontiers in Cell and Developmental Biology

Evaluating Enhancer Function and Transcription

(2020) Andrew Field et al.   Annual Review of Biochemistry

Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair

(2020) Yuka Nakazawa et al.   CELL

Regulation of the RNAPII Pool Is Integral to the DNA Damage Response

(2020) Ana Tufegdžić Vidaković et al.   CELL

Spt5 phosphorylation and the Rtf1 Plus3 domain promote Rtf1 function through distinct mechanisms

(2020) Jennifer J. Chen et al.   MOLECULAR AND CELLULAR BIOLOGY

NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-Release

(2020) Yuki Aoi et al.   MOLECULAR CELL

Spt5-mediated enhancer transcription directly couples enhancer activation with physical promoter interaction

(2020) Johanna Fitz et al.   NATURE GENETICS

The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II

(2020) Yana van der Weegen et al.   Nature Communications

Genome-wide role of Rad26 in promoting transcription-coupled nucleotide excision repair in yeast chromatin

(2020) Mingrui Duan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Selective inhibition of CDK7 reveals high-confidence targets and new models for TFIIH function in transcription

(2020) Jenna K. Rimel et al.   GENES & DEVELOPMENT

Mechanisms of Transcription Elongation Factor DSIF (Spt4–Spt5)

(2020) Tim-Michael Decker   JOURNAL OF MOLECULAR BIOLOGY

Integrator Recruits Protein Phosphatase 2A to Prevent Pause Release and Facilitate Transcription Termination

(2020) Kai-Lieh Huang et al.   MOLECULAR CELL

Distinct Cdk9-phosphatase switches act at the beginning and end of elongation by RNA polymerase II

(2020) Pabitra K. Parua et al.   Nature Communications

Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription

(2020) Grace A. Rosen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase

(2020) Hai Zheng et al.   SCIENCE

Structural insight into nucleosome transcription by RNA polymerase II with elongation factors

(2019) Haruhiko Ehara et al.   SCIENCE

Evidence that Moderate Eviction of Spt5 and Promotion of Error-Free Transcriptional Bypass by Rad26 Facilitates Transcription Coupled Nucleotide Excision Repair

(2019) Kathiresan Selvam et al.   JOURNAL OF MOLECULAR BIOLOGY

Promoter-proximal pausing of RNA polymerase II: a nexus of gene regulation

(2019) Leighton Core et al.   GENES & DEVELOPMENT

A unified allosteric/torpedo mechanism for transcriptional termination on human protein-coding genes

(2019) Joshua D. Eaton et al.   GENES & DEVELOPMENT

Control of RNA Pol II Speed by PNUTS-PP1 and Spt5 Dephosphorylation Facilitates Termination by a “Sitting Duck Torpedo” Mechanism

(2019) Michael A. Cortazar et al.   MOLECULAR CELL

Transcription through the nucleosome

(2019) Tomoya Kujirai et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structural Basis for Transcript Elongation Control by NusG Family Universal Regulators

(2018) Jin Young Kang et al.   CELL

Regulation of RNA polymerase II processivity by Spt5 is restricted to a narrow window during elongation

(2018) Johanna Fitz et al.   EMBO JOURNAL

Enhancer transcription: what, where, when, and why?

(2018) Nathaniel D. Tippens et al.   GENES & DEVELOPMENT

Widespread transcriptional pausing and elongation control at enhancers

(2018) Telmo Henriques et al.   GENES & DEVELOPMENT

Transcriptional Pause Sites Delineate Stable Nucleosome-Associated Premature Polyadenylation Suppressed by U1 snRNP

(2018) Anthony C. Chiu et al.   MOLECULAR CELL

Born to run: control of transcription elongation by RNA polymerase II

(2018) Fei Xavier Chen et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Enhancer Logic and Mechanics in Development and Disease

(2018) Ryan Rickels et al.   TRENDS IN CELL BIOLOGY

A Cdk9–PP1 switch regulates the elongation–termination transition of RNA polymerase II

(2018) Pabitra K. Parua et al.   NATURE

Structure of activated transcription complex Pol II–DSIF–PAF–SPT6

(2018) Seychelle M. Vos et al.   NATURE

Structure and nucleic acid binding properties of KOW domains 4 and 6–7 of human transcription elongation factor DSIF

(2018) Philipp K. Zuber et al.   Scientific Reports

TFS and Spt4/5 accelerate transcription through archaeal histone-based chromatin

(2018) Travis J. Sanders et al.   MOLECULAR MICROBIOLOGY

Elongation/Termination Factor Exchange Mediated by PP1 Phosphatase Orchestrates Transcription Termination

(2018) Tea Kecman et al.   Cell Reports

Identification of Regions in the Spt5 Subunit of DRB Sensitivity-inducing Factor (DSIF) That Are Involved in Promoter-proximal Pausing

(2017) Yijun Qiu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Genome-wide Analysis of RNA Polymerase II Termination at Protein-Coding Genes

(2017) Carlo Baejen et al.   MOLECULAR CELL

Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation

(2017) Ameet Shetty et al.   MOLECULAR CELL

Cockayne’s Syndrome A and B Proteins Regulate Transcription Arrest after Genotoxic Stress by Promoting ATF3 Degradation

(2017) Alexey Epanchintsev et al.   MOLECULAR CELL

Structural basis for the initiation of eukaryotic transcription-coupled DNA repair

(2017) Jun Xu et al.   NATURE

Structure of a transcribing RNA polymerase II–DSIF complex reveals a multidentate DNA–RNA clamp

(2017) Carrie Bernecky et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The elongation factor Spt4/5 regulates RNA polymerase II transcription through the nucleosome

(2017) John B. Crickard et al.   NUCLEIC ACIDS RESEARCH

Structure of histone-based chromatin in Archaea

(2017) Francesca Mattiroli et al.   SCIENCE

Structure of the complete elongation complex of RNA polymerase II with basal factors

(2017) Haruhiko Ehara et al.   SCIENCE

PAF1 regulation of promoter-proximal pause release via enhancer activation

(2017) Fei Xavier Chen et al.   SCIENCE

Structure of a transcribing RNA polymerase II–DSIF complex reveals a multidentate DNA–RNA clamp

(2017) Carrie Bernecky et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

P-TEFb regulation of transcription termination factor Xrn2 revealed by a chemical genetic screen for Cdk9 substrates

(2016) Miriam Sansó et al.   GENES & DEVELOPMENT

Divergence of a conserved elongation factor and transcription regulation in budding and fission yeast

(2016) Gregory T. Booth et al.   GENOME RESEARCH

Biochemical Analysis of Yeast Suppressor of Ty 4/5 (Spt4/5) Reveals the Importance of Nucleic Acid Interactions in the Prevention of RNA Polymerase II Arrest

(2016) J. Brooks Crickard et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

NusG Is a Sequence-specific RNA Polymerase Pause Factor That Binds to the Non-template DNA within the Paused Transcription Bubble

(2016) Alexander V. Yakhnin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Histone Modification Domain of Paf1 Complex Subunit Rtf1 Directly Stimulates H2B Ubiquitylation through an Interaction with Rad6

(2016) S. Branden Van Oss et al.   MOLECULAR CELL

Ubiquitin-Dependent Turnover of MYC Antagonizes MYC/PAF1C Complex Accumulation to Drive Transcriptional Elongation

(2016) Laura A. Jaenicke et al.   MOLECULAR CELL

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II

(2016) Xiaodong Lu et al.   NUCLEIC ACIDS RESEARCH

PAF1, a Molecular Regulator of Promoter-Proximal Pausing by RNA Polymerase II

(2015) Fei Xavier Chen et al.   CELL

Structures and Functions of the Multiple KOW Domains of Transcription Elongation Factor Spt5

(2015) Peter A. Meyer et al.   MOLECULAR AND CELLULAR BIOLOGY

Integrator mediates the biogenesis of enhancer RNAs

(2015) Fan Lai et al.   NATURE

Getting up to speed with transcription elongation by RNA polymerase II

(2015) Iris Jonkers et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II–transcribed genes

(2015) Clélia Laitem et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A unified architecture of transcriptional regulatory elements

(2015) Robin Andersson et al.   TRENDS IN GENETICS

CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II–transcribed genes

(2015) Clélia Laitem et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Enhancer RNA Facilitates NELF Release from Immediate Early Genes

(2014) Katie Schaukowitch et al.   MOLECULAR CELL

Insights into how Spt5 functions in transcription elongation and repressing transcription coupled DNA repair

(2014) Wentao Li et al.   NUCLEIC ACIDS RESEARCH

DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes

(2014) Junichi Yamamoto et al.   Nature Communications

RNA Polymerase II transcription elongation and Pol II CTD Ser2 phosphorylation

(2014) Elizabeth A Bowman et al.   Nucleus

Control of Transcriptional Elongation

(2013) Hojoong Kwak et al.   Annual Review of Genetics

The Recruitment of theSaccharomyces cerevisiaePaf1 Complex to Active Genes Requires a Domain of Rtf1 That Directly Interacts with the Spt4-Spt5 Complex

(2013) Manasi K. Mayekar et al.   MOLECULAR AND CELLULAR BIOLOGY

Structural basis for Spt5-mediated recruitment of the Paf1 complex to chromatin

(2013) A. D. Wier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Precise Maps of RNA Polymerase Reveal How Promoters Direct Initiation and Pausing

(2013) H. Kwak et al.   SCIENCE

RNA Polymerase II Elongation Control

(2012) Qiang Zhou et al.   Annual Review of Biochemistry

The Spt4–Spt5 complex: A multi-faceted regulator of transcription elongation

(2012) Grant A. Hartzog et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

The many roles of the conserved eukaryotic Paf1 complex in regulating transcription, histone modifications, and disease states

(2012) Brett N. Tomson et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

TiF1-gamma plays an essential role in murine hematopoiesis and regulates transcriptional elongation of erythroid genes

(2012) Xiaoying Bai et al.   DEVELOPMENTAL BIOLOGY

Pol II CTD kinases Bur1 and Kin28 promote Spt5 CTR-independent recruitment of Paf1 complex

(2012) Hongfang Qiu et al.   EMBO JOURNAL

A Nexus for Gene Expression—Molecular Mechanisms of Spt5 and NusG in the Three Domains of Life

(2012) Finn Werner   JOURNAL OF MOLECULAR BIOLOGY

The Spt5 C-Terminal Region Recruits Yeast 3' RNA Cleavage Factor I

(2012) A. Mayer et al.   MOLECULAR AND CELLULAR BIOLOGY

Promoter-proximal pausing of RNA polymerase II: emerging roles in metazoans

(2012) Karen Adelman et al.   NATURE REVIEWS GENETICS

The super elongation complex (SEC) family in transcriptional control

(2012) Zhuojuan Luo et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

The yeast regulator of transcription protein Rtr1 lacks an active site and phosphatase activity

(2012) Kehui Xiang et al.   Nature Communications

Defining the Status of RNA Polymerase at Promoters

(2012) Leighton J. Core et al.   Cell Reports

Human Mediator Subunit MED26 Functions as a Docking Site for Transcription Elongation Factors

(2011) Hidehisa Takahashi et al.   CELL

Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity

(2011) Fuensanta W Martinez-Rucobo et al.   EMBO JOURNAL

Yeast Transcription Elongation Factor Spt5 Associates with RNA Polymerase I and RNA Polymerase II Directly

(2011) Olga V. Viktorovskaya et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Transcription Elongation Factor Spt5 Influences Transcription by RNA Polymerase I Positively and Negatively

(2011) Susan J. Anderson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Ser7 Phosphorylation of the CTD Recruits the RPAP2 Ser5 Phosphatase to snRNA Genes

(2011) Sylvain Egloff et al.   MOLECULAR CELL

Activation-Induced Cytidine Deaminase Targets DNA at Sites of RNA Polymerase II Stalling by Interaction with Spt5

(2010) Rushad Pavri et al.   CELL

c-Myc Regulates Transcriptional Pause Release

(2010) Peter B. Rahl et al.   CELL

The RNA Polymerase-associated Factor 1 Complex (Paf1C) Directly Increases the Elongation Rate of RNA Polymerase I and Is Required for Efficient Regulation of rRNA Synthesis

(2010) Yinfeng Zhang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif

(2010) Angela Hirtreiter et al.   NUCLEIC ACIDS RESEARCH

RNA polymerase and transcription elongation factor Spt4/5 complex structure

(2010) B. J. Klein et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Histone H3K27ac separates active from poised enhancers and predicts developmental state

(2010) M. P. Creyghton et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex

(2010) A. Missra et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation

(2009) Y. Chen et al.   GENES & DEVELOPMENT

The C-terminal Repeat Domain of Spt5 Plays an Important Role in Suppression of Rad26-independent Transcription Coupled Repair

(2009) Baojin Ding et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phosphorylation of the Transcription Elongation Factor Spt5 by Yeast Bur1 Kinase Stimulates Recruitment of the PAF Complex

(2009) Y. Liu et al.   MOLECULAR AND CELLULAR BIOLOGY

Rtr1 Is a CTD Phosphatase that Regulates RNA Polymerase II during the Transition from Serine 5 to Serine 2 Phosphorylation

(2009) Amber L. Mosley et al.   MOLECULAR CELL

A capping-independent function of MePCE in stabilizing 7SK snRNA and facilitating the assembly of 7SK snRNP

(2009) Y. Xue et al.   NUCLEIC ACIDS RESEARCH

Control of transcriptional elongation and cotranscriptional histone modification by the yeast BUR kinase substrate Spt5

(2009) K. Zhou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Paf1 complex is required for efficient transcription elongation by RNA polymerase I

(2009) Y. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3′ mRNA processing factors

(2009) Orit Rozenblatt-Rosen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct Interactions between the Paf1 Complex and a Cleavage and Polyadenylation Factor Are Revealed by Dissociation of Paf1 from RNA Polymerase II

(2008) Kristen Nordick et al.   EUKARYOTIC CELL

PP2B and PP1  cooperatively disrupt 7SK snRNP to release P-TEFb for transcription in response to Ca2+ signaling

(2008) R. Chen et al.   GENES & DEVELOPMENT

Transcription-coupled DNA repair: two decades of progress and surprises

(2008) Philip C. Hanawalt et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Identification of Spt5 Target Genes in Zebrafish Development Reveals Its Dual Activity In Vivo

(2008) Keerthi Krishnan et al.   PLoS One