Article
Biochemistry & Molecular Biology
Suthida Panwong, Methi Wathikthinnakon, Thida Kaewkod, Nunghathai Sawasdee, Yingmanee Tragoolpua, Pa-thai Yenchitsomanus, Aussara Panya
Summary: Immunotherapy utilizing immune functions is a promising strategy for cancer treatment, and the bioactive compound cordycepin has been shown to enhance the susceptibility of cancer cells to NK cell cytotoxicity, possibly through the TRAIL signaling pathway. This study provides evidence that cordycepin can sensitize cancer cells to NK cell cytotoxicity, supporting its potential as an alternative immunomodulating agent.
Article
Pharmacology & Pharmacy
Takashi Nakamura, Taisei Nakade, Yusuke Sato, Hideyoshi Harashima
Summary: This study reports the use of CL1H6-LNP, a lipid nanoparticle, for delivering mRNA to NK-92 cells. Compared to the benchmark DLin-MC3-DMA based LNP, the CL1H6-LNP showed higher mRNA expression intensity and 100% cell transfection efficiency. The efficient mRNA delivery by CL1H6-LNP is attributed to its high affinity for NK-92 cells and rapid fusion with the endosomal membrane, highlighting its potential as a non-viral vector for modifying NK-92 cell functions.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Multidisciplinary Sciences
Rebecca Kotzur, Alexandra Duev-Cohen, Inbal Kol, Adi Reches, Ofer Mandelboim, Natan Stein
Summary: NK-92 cells, a cell-based immunotherapy, are currently being tested in clinical trials for various cancer types. They are an important research tool due to their similarity to natural killer cells. This study shows that NK-92 cells can grow in the user-friendly RPMI medium supplemented with IL-2, resulting in improved metabolic activity and replication rates. These findings will facilitate more researchers to work with and manipulate this cell line.
Article
Oncology
Mohammadreza Dastouri, Nil Kilic, Humeyra Yilmaz
Summary: Research on immunotherapy in breast cancer treatment has recently gained importance. Natural killer (NK) cells, specifically the NK-92 cells stimulated with anti-CD226 antibodies (sNK-92), have been found to effectively target and kill breast cancer cells. The cytotoxic effects of sNK-92 cells on MDA-MB-231 triple-negative breast cancer cells were stronger than regular NK-92 cells. Additionally, sNK-92 cells secreted more granzyme B and induced apoptosis in breast cancer cells, while not affecting normal breast cells. These findings suggest the potential use of CD226-stimulated NK-92 cells in breast cancer immunotherapy.
Article
Oncology
Nil Kilic, Mohammadreza Dastouri, Irfan Kandemir, Erkan Yilmaz
Summary: In this study, anti-KIR2DL4 was used to stimulate NK-92 cells and enhance their cytotoxicity on breast cancer cells. sNK-92 cells showed higher cytotoxic activity on breast cancer cells through apoptosis pathways. However, their effect on normal breast cells was limited.
Article
Pharmacology & Pharmacy
Yan Peng, Wenqing Zhang, Yufeng Chen, Louqian Zhang, Hongyu Shen, Zheyue Wang, Shuning Tian, Xiaohui Yang, Daixun Cui, Yiting He, Xinxia Chang, Zhenqing Feng, Qi Tang, Yuan Mao
Summary: This study explored the potential application of CAR-modified NK cells targeting c-Met in LUAD. The expression status and prognostic role of c-Met in LUAD were investigated. Four types of c-Met-CAR structures were designed and prepared. The results showed that DAP10 is a potent stimulator for NK cell activation and CCN4-based immunotherapy could be a promising strategy for the treatment of c-Met-positive LUAD.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Oncology
Changqing Pan, You Zhai, Chen Wang, Zhiyi Liao, Di Wang, Mingchen Yu, Fan Wu, Yiyun Yin, Zhongfang Shi, Guanzhang Li, Tao Jiang, Wei Zhang
Summary: This study investigated the expression of poliovirus receptor in IDH wild-type glioblastoma patients and developed chimeric antigen receptor T cells targeting poliovirus receptor. It was found that poliovirus receptor-based chimeric antigen receptor T cells were capable of killing glioma stem cells and suppressing tumor recurrence when combined with NK-92 cells.
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
(2023)
Article
Chemistry, Analytical
Bo Hu, Qin Zhang, Xiaonan Gao, Kehua Xu, Bo Tang
Summary: By designing a multicolor fluorescent nanoprobe Cas-NP, the activation of caspases-1/3/4 during pyroptosis can be accurately monitored, revealing the pathways of pyroptosis under different stimuli. Through studying the pyroptosis process in cancer cells, it was first discovered that LPS stimulation can induce caspase-3 activation, and the activation sequence of caspases-1/3/4 was verified.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Bihui Cao, Manting Liu, Jingjun Huang, Jingwen Zhou, Junping Li, Hui Lian, Wensou Huang, Yongjian Guo, Shuo Yang, Liteng Lin, Mingyue Cai, Cheng Zhi, Jingqiang Wu, Licong Liang, Yuling Hu, Hong Hu, Jinping He, Baoxia Liang, Qi Zhao, Kangshun Zhu
Summary: The study demonstrated that MSLN-CAR NK cells exhibit strong antitumor activity and can effectively kill MSLN-positive gastric cancer cells, showing promising therapeutic potential for gastric cancer treatment. Moreover, MSLN-CAR NK cells also significantly prolonged the survival of tumor-bearing mice in both subcutaneous and intraperitoneal tumor models, indicating their potent antitumor effects in vivo. Additionally, the observations of potent antitumor effects and significant NK cell infiltration in patient-derived xenografts treated with MSLN-CAR NK cells further supported the therapeutic efficacy of MSLN-CAR NK cells for gastric cancer.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Zhepei Xie, Yan Fu, Wen-song Tan, Haibo Cai
Summary: This study successfully designed a serum-free medium EM-FA with optimized fatty acid additives to facilitate rapid expansion of NK-92 cells and enhance their cellular metabolism. Fatty acids upregulated oxidative phosphorylation levels, improving the energy metabolism of NK-92 cells.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nadia Mensali, Pierre Dillard, Artem Fayzullin, Hakan Koksal, Gustav Gaudernack, Gunnar Kvalheim, Else Marit Inderberg, Sebastien Walchli
Summary: The success of adoptive cell therapy relies on the ability of immune cells to persist and function optimally in the tumor microenvironment. Novel genetic strategies to manipulate the PD1/PD-L1 axis can improve antitumor immunity and reveal new targets through PD-L1 positivity.
Review
Immunology
Ariel Ramirez-Labrada, Cecilia Pesini, Llipsy Santiago, Sandra Hidalgo, Adanays Calvo-Perez, Carmen Onate, Alejandro Andres-Tovar, Marcela Garzon-Tituana, Iratxe Uranga-Murillo, Maykel A. Arias, Eva M. Galvez, Julian Pardo
Summary: In this article, the available experimental evidence on how NK cells kill target cells is reviewed and discussed, with a special focus on the different cell death modalities that have been found to be activated during NK cell-mediated cytotoxicity, including apoptosis and more inflammatory pathways like necroptosis and pyroptosis. Based on this new evidence, NK cell-induced cell death is proposed as a new regulatory mechanism linking innate immune response with the activation of tumor adaptive T cell responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Pengyun Li, Qiaohong Lin, Shiyang Sun, Ning Yang, Yu Xia, Shengjie Cao, Wenjuan Zhang, Qian Li, Haoxin Guo, Maoxiang Zhu, Yilong Wang, Zhibing Zheng, Song Li
Summary: This study identified the potential of dual targeting CB1 and ferroptosis as a promising therapeutic strategy for TNBC. By inhibiting CB1 and regulating lipid metabolism, the effectiveness of ferroptosis in TNBC cells can be enhanced, leading to tumor growth inhibition.
CELL DEATH & DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Paolo Sportoletti, Filomena De Falco, Beatrice Del Papa, Stefano Baldoni, Valerio Guarente, Andrea Marra, Erica Dorillo, Chiara Rompietti, Francesco Maria Adamo, Loredana Ruggeri, Mauro Di Ianni, Emanuela Rosati
Summary: Key features of CLL include immune system defects and leukemic cells’ ability to evade immune defenses, leading to increased susceptibility to infections and disease progression. The role of NK cells in CLL is less understood, but evidence suggests that NK cell dysfunctions in CLL mainly depend on escape mechanisms employed by leukemic cells. NK cells, with their preserved ADCC function and reversible dysfunctions, are an attractive source for novel immunotherapeutic strategies in CLL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Ondrej Venglar, Julio Rodriguez Bago, Benjamin Motais, Roman Hajek, Tomas Jelinek
Summary: NK cells are a subset of lymphocytes that have cytotoxic and suppressor activity against virus-infected cells and cancer cells. They have potential in targeted immunotherapy for solid and hematological malignancies, including multiple myeloma. However, NK cells are impaired by various cancer defense mechanisms, leading to deficiencies in maturation, chemotaxis, target recognition, and killing.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Thidarath Rattanaburee, Mutita Junking, Aussara Panya, Nunghathai Sawasdee, Pucharee Songprakhon, Aroonroong Suttitheptumrong, Thawornchai Limjindaporn, Guy Haegeman, Pa-thai Yenchitsomanus
ANTIVIRAL RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Aussara Panya, Nunghathai Sawasdee, Mutita Junking, Chatchawan Srisawat, Kiattawee Choowongkomon, Pa-thai Yenchitsomanus
CHEMICAL BIOLOGY & DRUG DESIGN
(2015)
Article
Nanoscience & Nanotechnology
Krongkaew Navakul, Chompunuch Warakulwit, Pa-thai Yenchitsomanus, Aussara Panya, Peter A. Lieberzeit, Chak Sangma
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2017)
Article
Biotechnology & Applied Microbiology
Chutamas Thepmalee, Aussara Panya, Mutita Junking, Thaweesak Chieochansin, Pa-thai Yenchitsomanus
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2018)
Article
Biochemistry & Molecular Biology
Aussara Panya, Kunan Bangphoomi, Kiattawee Choowongkomon, Pa-thai Yenchitsomanus
CHEMICAL BIOLOGY & DRUG DESIGN
(2014)
Article
Biotechnology & Applied Microbiology
N. Saokaew, O. Poungpair, A. Panya, M. Tarasuk, N. Sawasdee, T. Limjindaporn, W. Chaicumpa, P. Yenchitsomanus
LETTERS IN APPLIED MICROBIOLOGY
(2014)
Article
Multidisciplinary Sciences
Aussara Panya, Nunghathai Sawasdee, Chatchawan Srisawat, Pa-thai Yenchitsomanus, Chayanon Peerapittayamongkol
Article
Infectious Diseases
Kanyaluck Jantakee, Panchika Prangkio, Aussara Panya, Yingmanee Tragoolpua
Summary: HSV infections are common worldwide and can cause life-threatening diseases. Silk extracts from silkworm cocoons, particularly the non-sericin extract, show potential antiviral activity against HSV-1 and HSV-2, as well as virucidal effects. Treatment with non-sericin extract in HSV-2-infected cells can reduce cell death and inflammation.
Article
Virology
Patta Phumesin, Mutita Junking, Aussara Panya, Petlada Yongpitakwattana, Sansanee Noisakran, Thawornchai Limjindaporn, Pa-thai Yenchitsomanus
ARCHIVES OF VIROLOGY
(2018)